3wug - Revision history

OCA at 13:29, 8 November 2023

2023-11-08T13:29:36Z

←Older revision		Revision as of 13:29, 8 November 2023
Line 3:		Line 3:
	<StructureSection load='3wug' size='340' side='right'caption='[[3wug]], [[Resolution\|resolution]] 1.88Å' scene=''>		<StructureSection load='3wug' size='340' side='right'caption='[[3wug]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wug]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chainia_sp~~. ~~Chainia~~ sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wug]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WUG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~, ~~<scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.88Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3wub\|3wub]], [[3wue\|3wue]], [[3wuf\|3wuf]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PRD_900116:4beta-beta-xylobiose'>PRD_900116</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><~~td class="sblockDat">xynAS9 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Chainia sp.])</td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wug OCA], [https://pdbe.org/3wug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wug RCSB], [https://www.ebi.ac.uk/pdbsum/3wug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wug ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wug OCA], [https://pdbe.org/3wug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wug RCSB], [https://www.ebi.ac.uk/pdbsum/3wug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wug ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/XYNA_STRSQ XYNA_STRSQ]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chainia sp]]
-	[[Category: Endo-1,4-beta-xylanase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen~~, C C~~]]	+	[[Category: Streptomyces sp]]
-	[[Category: Gao, J]]	+	[[Category: Chen CC]]
-	[[Category: Guo~~, R T~~]]	+	[[Category: Gao J]]
-	[[Category: Han, X]]	+	[[Category: Guo RT]]
-	[[Category: Huang~~, C H~~]]	+	[[Category: Han X]]
-	[[Category: Ko~~, T P~~]]	+	[[Category: Huang CH]]
-	[[Category: Lv, P]]	+	[[Category: Ko TP]]
-	[[Category: Peng, W]]	+	[[Category: Lv P]]
-	[[Category: Yang~~, Y Y~~]]	+	[[Category: Peng W]]
-	[[Category: Zheng, Y]]	+	[[Category: Yang YY]]
-	~~[[Category: 4-xylanase]]~~	+	[[Category: Zheng Y]]
-	~~[[Category: Beta-1]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protein engineering]]~~	+
-	~~[[Category: Protein rigidity]]~~	+
-	~~[[Category: Thermozyme~~]]	+

OCA at 05:31, 10 August 2022

2022-08-10T05:31:58Z

←Older revision		Revision as of 05:31, 10 August 2022
Line 1:		Line 1:

	==The mutant crystal structure of b-1,4-Xylanase (XynAS9_V43P/G44E) with xylobiose from Streptomyces sp. 9==		==The mutant crystal structure of b-1,4-Xylanase (XynAS9_V43P/G44E) with xylobiose from Streptomyces sp. 9==
-	<StructureSection load='3wug' size='340' side='right' caption='[[3wug]], [[Resolution\|resolution]] 1.88Å' scene=''>	+	<StructureSection load='3wug' size='340' side='right'caption='[[3wug]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wug]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chainia_sp. Chainia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wug]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chainia_sp. Chainia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WUG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~XYP~~:~~BETA-D-XYLOPYRANOSE~~'>~~XYP~~</scene>, <scene name='pdbligand=ZN:~~ZINC+ION~~'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wub\|3wub]], [[3wue\|3wue]], [[3wuf\|3wuf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wub\|3wub]], [[3wue\|3wue]], [[3wuf\|3wuf]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xynAS9 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Chainia sp.])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xynAS9 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Chainia sp.])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3wug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wug OCA], [~~http~~://pdbe.org/3wug PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3wug RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3wug PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3wug ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wug OCA], [https://pdbe.org/3wug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wug RCSB], [https://www.ebi.ac.uk/pdbsum/3wug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wug ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	[[Category: Chainia sp]]		[[Category: Chainia sp]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
		+	[[Category: Large Structures]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]
	[[Category: Gao, J]]		[[Category: Gao, J]]

OCA at 09:08, 21 February 2019

2019-02-21T09:08:18Z

←Older revision		Revision as of 09:08, 21 February 2019
Line 3:		Line 3:
	<StructureSection load='3wug' size='340' side='right' caption='[[3wug]], [[Resolution\|resolution]] 1.88Å' scene=''>		<StructureSection load='3wug' size='340' side='right' caption='[[3wug]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wug]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chainia_sp. Chainia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WUG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:~~ZINC+ION~~'>ZN</scene>, <scene name='pdbligand=~~XYP~~:~~BETA-D-XYLOPYRANOSE~~'>~~XYP~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wub\|3wub]], [[3wue\|3wue]], [[3wuf\|3wuf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wub\|3wub]], [[3wue\|3wue]], [[3wuf\|3wuf]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xynAS9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Chainia sp.])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wug OCA], [http://pdbe.org/3wug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wug RCSB], [http://www.ebi.ac.uk/pdbsum/3wug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wug ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wug OCA], [http://pdbe.org/3wug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wug RCSB], [http://www.ebi.ac.uk/pdbsum/3wug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wug ProSAT]</span></td></tr>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chainia sp]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]

OCA at 09:09, 11 August 2016

2016-08-11T09:09:20Z

←Older revision		Revision as of 09:09, 11 August 2016
Line 1:		Line 1:
		+
	==The mutant crystal structure of b-1,4-Xylanase (XynAS9_V43P/G44E) with xylobiose from Streptomyces sp. 9==		==The mutant crystal structure of b-1,4-Xylanase (XynAS9_V43P/G44E) with xylobiose from Streptomyces sp. 9==
	<StructureSection load='3wug' size='340' side='right' caption='[[3wug]], [[Resolution\|resolution]] 1.88Å' scene=''>		<StructureSection load='3wug' size='340' side='right' caption='[[3wug]], [[Resolution\|resolution]] 1.88Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wub\|3wub]], [[3wue\|3wue]], [[3wuf\|3wuf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wub\|3wub]], [[3wue\|3wue]], [[3wuf\|3wuf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wug RCSB], [http://www.ebi.ac.uk/pdbsum/3wug PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wug OCA], [http://pdbe.org/3wug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wug RCSB], [http://www.ebi.ac.uk/pdbsum/3wug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wug ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3wug" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:17, 25 January 2015

2015-01-25T10:17:37Z

←Older revision		Revision as of 10:17, 25 January 2015
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
-	[[Category: Chen, C C.]]	+	[[Category: Chen, C C]]
-	[[Category: Gao, J.]]	+	[[Category: Gao, J]]
-	[[Category: Guo, R T.]]	+	[[Category: Guo, R T]]
-	[[Category: Han, X.]]	+	[[Category: Han, X]]
-	[[Category: Huang, C H.]]	+	[[Category: Huang, C H]]
-	[[Category: Ko, T P.]]	+	[[Category: Ko, T P]]
-	[[Category: Lv, P.]]	+	[[Category: Lv, P]]
-	[[Category: Peng, W.]]	+	[[Category: Peng, W]]
-	[[Category: Yang, Y Y.]]	+	[[Category: Yang, Y Y]]
-	[[Category: Zheng, Y.]]	+	[[Category: Zheng, Y]]
	[[Category: 4-xylanase]]		[[Category: 4-xylanase]]
	[[Category: Beta-1]]		[[Category: Beta-1]]

OCA at 11:21, 29 October 2014

2014-10-29T11:21:39Z

←Older revision		Revision as of 11:21, 29 October 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==The mutant crystal structure of b-1,4-Xylanase (XynAS9_V43P/G44E) with xylobiose from Streptomyces sp. 9==
		+	<StructureSection load='3wug' size='340' side='right' caption='[[3wug]], [[Resolution\|resolution]] 1.88Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3wug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WUG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wub\|3wub]], [[3wue\|3wue]], [[3wuf\|3wuf]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wug RCSB], [http://www.ebi.ac.uk/pdbsum/3wug PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The glycoside hydrolase 10 (GH10) xylanase from Streptomyces sp. 9 (XynAS9) can operate in a broad range of pH and temperature, and thus is a potential candidate for commercial applications. Recently, we engineered XynAS9 via mutating several residues in accordance with the consensus sequences of GH10 thermophilic xylanases in an attempt to improve the enzyme thermostability and thermotolerance. The most promising effects were observed in the double mutant V81P/G82E. In order to investigate the molecular mechanism of the improved thermal profile of XynAS9, complex crystal structures of the wild type (WT) and mutant (MT) enzyme were solved at 1.88-2.05A resolution. The structures reveal a classical GH10 (beta/alpha)8 TIM-barrel fold. In MT XynAS9, E82 forms several interactions to its neighboring residues, which might aid in stabilizing the local structure. Furthermore, the MT structure showed lower B factors for individual residues compared to the WT structure, reflecting the increased MT protein rigidity. Analyses of the XynAS9 structures also delineate the detailed enzyme-substrate interaction network. More importantly, possible explanations for the enhanced thermal profiles of MT XynAS9 are proposed, which may be a useful strategy for enzyme engineering in the future.

-	~~The entry 3wug is ON HOLD until Paper Publication~~	+	Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability.,Chen CC, Luo H, Han X, Lv P, Ko TP, Peng W, Huang CH, Wang K, Gao J, Zheng Y, Yang Y, Zhang J, Yao B, Guo RT J Biotechnol. 2014 Sep 3;189C:175-182. doi: 10.1016/j.jbiotec.2014.08.030. PMID:25193708<ref>PMID:25193708</ref>

-	~~Authors~~: Chen, C.C., ~~Han, X., Lv, P~~., ~~Ko,~~ T.~~P., Peng~~, W., Huang, C.H., ~~Zheng, Y~~., ~~Gao, J~~., Yang, Y.Y., ~~Guo, R.T~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~The mutant crystal structure of b~~-1~~,4-Xylanase (XynAS9_V43P/G44E) with xylobiose from Streptomyces sp. 9~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Endo-1,4-beta-xylanase]]
		+	[[Category: Chen, C C.]]
		+	[[Category: Gao, J.]]
		+	[[Category: Guo, R T.]]
		+	[[Category: Han, X.]]
		+	[[Category: Huang, C H.]]
		+	[[Category: Ko, T P.]]
		+	[[Category: Lv, P.]]
		+	[[Category: Peng, W.]]
		+	[[Category: Yang, Y Y.]]
		+	[[Category: Zheng, Y.]]
		+	[[Category: 4-xylanase]]
		+	[[Category: Beta-1]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protein engineering]]
		+	[[Category: Protein rigidity]]
		+	[[Category: Thermozyme]]

OCA at 07:35, 24 September 2014

2014-09-24T07:35:09Z

←Older revision		Revision as of 07:35, 24 September 2014
Line 5:		Line 5:
	Authors: Chen, C.C., Han, X., Lv, P., Ko, T.P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.		Authors: Chen, C.C., Han, X., Lv, P., Ko, T.P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.

-	Description: ~~Mutant~~ crystal ~~structural~~ of ~~a glycoside hydrolase~~ with ~~ligands~~	+	Description: The mutant crystal structure of b-1,4-Xylanase (XynAS9_V43P/G44E) with xylobiose from Streptomyces sp. 9

OCA at 05:32, 25 June 2014

2014-06-25T05:32:39Z

←Older revision		Revision as of 05:32, 25 June 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3wug is ON HOLD	+	The entry 3wug is ON HOLD until Paper Publication

	Authors: Chen, C.C., Han, X., Lv, P., Ko, T.P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.		Authors: Chen, C.C., Han, X., Lv, P., Ko, T.P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.

	Description: Mutant crystal structural of a glycoside hydrolase with ligands		Description: Mutant crystal structural of a glycoside hydrolase with ligands

OCA at 07:17, 7 May 2014

2014-05-07T07:17:19Z

←Older revision		Revision as of 07:17, 7 May 2014
Line 3:		Line 3:
	The entry 3wug is ON HOLD		The entry 3wug is ON HOLD

-	Authors: Chen, C.C, Han, X., Lv, P., Ko, T,P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.	+	Authors: Chen, C.C., Han, X., Lv, P., Ko, T.P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.

-	Description: ~~mutant~~ crystal structural of a glycoside hydrolase with ligands	+	Description: Mutant crystal structural of a glycoside hydrolase with ligands

OCA: Protected "3wug" [edit=sysop:move=sysop]

2014-04-30T05:43:19Z

Protected "3wug" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:43, 30 April 2014