3x0g - Revision history

OCA at 13:37, 8 November 2023

2023-11-08T13:37:50Z

←Older revision		Revision as of 13:37, 8 November 2023
Line 1:		Line 1:

	==Crystal structure of the ectodomain of African green monkey CD81 large extracellular loop (agmCD81-LEL)==		==Crystal structure of the ectodomain of African green monkey CD81 large extracellular loop (agmCD81-LEL)==
-	<StructureSection load='3x0g' size='340' side='right'caption='[[3x0g]]' scene=''>	+	<StructureSection load='3x0g' size='340' side='right'caption='[[3x0g]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3x0g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlorocebus_sabaeus Chlorocebus sabaeus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [https://pdbe.org/3x0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [https://www.ebi.ac.uk/pdbsum/3x0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0g ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.899Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [https://pdbe.org/3x0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [https://www.ebi.ac.uk/pdbsum/3x0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0g ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0D9RBI8_CHLSB A0A0D9RBI8_CHLSB]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hepatitis C virus (HCV) infection is one of the leading causes of chronic liver diseases; however, HCV vaccine remains unavailable to date. One main obstacle is the lack of an efficient small animal model. Cluster of differentiation 81 (CD81) is an essential entry coreceptor for HCV species specificity to human, though the underlying mechanisms are yet to be fully elucidated. We performed structural, biophysical, and virologic studies on HCV nonpermissive CD81s from mouse and African green monkey [mouse cluster of differentiation 81 (mCD81) and African green monkey cluster of differentiation 81 (agmCD81)] and compared with human cluster of differentiation 81 (hCD81). We discovered an intramolecular hydrogen bond (Gln188-Nepsilon2-H: Glu196-Oepsilon2, 2 A, 124 degrees ) within the large extracellular loop (LEL) of mCD81 and a salt bridge (Lys188-Nzeta: Asp196-Odelta2, 2.4 A) within agmCD81-LEL between 188-196 residues. This structural feature is missing in hCD81. We demonstrated that the introduction of a single 188-196 bond to hCD81 impaired its binding affinity to HCV envelope glycoprotein 2 (HCV E2) and significantly decreased HCV pseudoviral particle (HCVpp) entry efficiency (4.92- to 8.42-fold) and cell culture-grown HCV (HCVcc) infectivity (4.55-fold), despite the availability of Phe186. For HCV nonpermissive CD81s, the introduction of Phe186 by Leu186F substitution alone was insufficient to confer HCV permissiveness. The disruption of the original 188-196 bond and Leu186F substitution were both required for potent binding to HCV E2 HCVpp entry efficiency and HCVcc infectivity. Our structural and biophysical analyses suggest that the intramolecular 188-196 bond restricts the intrinsic conformational dynamics of D-helix of CD81-LEL, which is essential for HCV entry, thus impairs HCV permissiveness. Our findings reveal a novel molecular determinant for HCV entry in addition to the well-characterized Phe186 and provide further guideline for selecting an HCV small animal model.-Yang, W., Zhang, M., Chi, X., Liu, X., Qin, B., Cui, S. An intramolecular bond at cluster of differentiation 81 ectodomain is important for hepatitis C virus entry.
		+
		+	An intramolecular bond at cluster of differentiation 81 ectodomain is important for hepatitis C virus entry.,Yang W, Zhang M, Chi X, Liu X, Qin B, Cui S FASEB J. 2015 Jun 26. pii: fj.15-272880. PMID:26116703<ref>PMID:26116703</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3x0g" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[CD81\|CD81]]		*[[CD81\|CD81]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chlorocebus sabaeus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cui S]]		[[Category: Cui S]]
	[[Category: Zhang M]]		[[Category: Zhang M]]

OCA at 10:40, 31 August 2022

2022-08-31T10:40:16Z

←Older revision		Revision as of 10:40, 31 August 2022
Line 1:		Line 1:

	==Crystal structure of the ectodomain of African green monkey CD81 large extracellular loop (agmCD81-LEL)==		==Crystal structure of the ectodomain of African green monkey CD81 large extracellular loop (agmCD81-LEL)==
-	<StructureSection load='3x0g' size='340' side='right'caption='[[3x0g]]~~, [[Resolution\|resolution]] 1.90Å~~' scene=''>	+	<StructureSection load='3x0g' size='340' side='right'caption='[[3x0g]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[3x0g]] is a 1 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0G FirstGlance]. <br>
-	</td></tr>~~<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [https://pdbe.org/3x0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [https://www.ebi.ac.uk/pdbsum/3x0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0g ProSAT]</span></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g8q\|1g8q]], [[1iv5\|1iv5]], [[3x0e\|3x0e]], [[3x0f\|3x0f]]</div></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [https://pdbe.org/3x0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [https://www.ebi.ac.uk/pdbsum/3x0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0g ProSAT]</span></td></tr>	+
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Hepatitis C virus (HCV) infection is one of the leading causes of chronic liver diseases; however, HCV vaccine remains unavailable to date. One main obstacle is the lack of an efficient small animal model. Cluster of differentiation 81 (CD81) is an essential entry coreceptor for HCV species specificity to human, though the underlying mechanisms are yet to be fully elucidated. We performed structural, biophysical, and virologic studies on HCV nonpermissive CD81s from mouse and African green monkey [mouse cluster of differentiation 81 (mCD81) and African green monkey cluster of differentiation 81 (agmCD81)] and compared with human cluster of differentiation 81 (hCD81). We discovered an intramolecular hydrogen bond (Gln188-Nepsilon2-H: Glu196-Oepsilon2, 2 A, 124 degrees ) within the large extracellular loop (LEL) of mCD81 and a salt bridge (Lys188-Nzeta: Asp196-Odelta2, 2.4 A) within agmCD81-LEL between 188-196 residues. This structural feature is missing in hCD81. We demonstrated that the introduction of a single 188-196 bond to hCD81 impaired its binding affinity to HCV envelope glycoprotein 2 (HCV E2) and significantly decreased HCV pseudoviral particle (HCVpp) entry efficiency (4.92- to 8.42-fold) and cell culture-grown HCV (HCVcc) infectivity (4.55-fold), despite the availability of Phe186. For HCV nonpermissive CD81s, the introduction of Phe186 by Leu186F substitution alone was insufficient to confer HCV permissiveness. The disruption of the original 188-196 bond and Leu186F substitution were both required for potent binding to HCV E2 HCVpp entry efficiency and HCVcc infectivity. Our structural and biophysical analyses suggest that the intramolecular 188-196 bond restricts the intrinsic conformational dynamics of D-helix of CD81-LEL, which is essential for HCV entry, thus impairs HCV permissiveness. Our findings reveal a novel molecular determinant for HCV entry in addition to the well-characterized Phe186 and provide further guideline for selecting an HCV small animal model.-Yang, W., Zhang, M., Chi, X., Liu, X., Qin, B., Cui, S. An intramolecular bond at cluster of differentiation 81 ectodomain is important for hepatitis C virus entry.
-
-	An intramolecular bond at cluster of differentiation 81 ectodomain is important for hepatitis C virus entry.,Yang W, Zhang M, Chi X, Liu X, Qin B, Cui S FASEB J. 2015 Jun 26. pii: fj.15-272880. PMID:26116703<ref>PMID:26116703</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3x0g" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[CD81\|CD81]]		*[[CD81\|CD81]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cui, S]]	+	[[Category: Cui S]]
-	[[Category: Zhang, M]]	+	[[Category: Zhang M]]
-	~~[[Category: Activation]]~~	+
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Differentiation]]~~	+
-	~~[[Category: Disulfide bond]]~~	+
-	~~[[Category: Helical bundle]]~~	+
-	~~[[Category: Immune cell adhesion]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Morphology]]~~	+
-	~~[[Category: Proliferation~~]]	+

OCA at 05:00, 25 August 2022

2022-08-25T05:00:56Z

←Older revision		Revision as of 05:00, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='3x0g' size='340' side='right'caption='[[3x0g]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3x0g' size='340' side='right'caption='[[3x0g]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3x0g]] is a 1 chain structure ~~with sequence from [https://en.wikipedia.org/wiki/Chlsb Chlsb]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3x0g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g8q\|1g8q]], [[1iv5\|1iv5]], [[3x0e\|3x0e]], [[3x0f\|3x0f]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g8q\|1g8q]], [[1iv5\|1iv5]], [[3x0e\|3x0e]], [[3x0f\|3x0f]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CD81 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=60711 CHLSB])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [https://pdbe.org/3x0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [https://www.ebi.ac.uk/pdbsum/3x0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [https://pdbe.org/3x0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [https://www.ebi.ac.uk/pdbsum/3x0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0g ProSAT]</span></td></tr>
	</table>		</table>
Line 25:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chlsb]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cui, S]]		[[Category: Cui, S]]

OCA at 05:37, 10 August 2022

2022-08-10T05:37:43Z

←Older revision		Revision as of 05:37, 10 August 2022
Line 1:		Line 1:

	==Crystal structure of the ectodomain of African green monkey CD81 large extracellular loop (agmCD81-LEL)==		==Crystal structure of the ectodomain of African green monkey CD81 large extracellular loop (agmCD81-LEL)==
-	<StructureSection load='3x0g' size='340' side='right' caption='[[3x0g]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3x0g' size='340' side='right'caption='[[3x0g]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3x0g]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chlsb Chlsb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3X0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3x0g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlsb Chlsb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g8q\|1g8q]], [[1iv5\|1iv5]], [[3x0e\|3x0e]], [[3x0f\|3x0f]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g8q\|1g8q]], [[1iv5\|1iv5]], [[3x0e\|3x0e]], [[3x0f\|3x0f]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CD81 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=60711 CHLSB])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CD81 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=60711 CHLSB])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [~~http~~://pdbe.org/3x0g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3x0g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0g ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [https://pdbe.org/3x0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [https://www.ebi.ac.uk/pdbsum/3x0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0g ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 3x0g" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3x0g" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[CD81\|CD81]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Chlsb]]		[[Category: Chlsb]]
		+	[[Category: Large Structures]]
	[[Category: Cui, S]]		[[Category: Cui, S]]
	[[Category: Zhang, M]]		[[Category: Zhang, M]]

OCA at 16:31, 27 February 2019

2019-02-27T16:31:20Z

←Older revision		Revision as of 16:31, 27 February 2019
Line 3:		Line 3:
	<StructureSection load='3x0g' size='340' side='right' caption='[[3x0g]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3x0g' size='340' side='right' caption='[[3x0g]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3x0g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3x0g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlsb Chlsb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X0G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g8q\|1g8q]], [[1iv5\|1iv5]], [[3x0e\|3x0e]], [[3x0f\|3x0f]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g8q\|1g8q]], [[1iv5\|1iv5]], [[3x0e\|3x0e]], [[3x0f\|3x0f]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CD81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=60711 CHLSB])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [http://pdbe.org/3x0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [http://www.ebi.ac.uk/pdbsum/3x0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [http://pdbe.org/3x0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [http://www.ebi.ac.uk/pdbsum/3x0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0g ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chlsb]]
	[[Category: Cui, S]]		[[Category: Cui, S]]
	[[Category: Zhang, M]]		[[Category: Zhang, M]]

OCA at 12:09, 12 April 2017

2017-04-12T12:09:09Z

←Older revision		Revision as of 12:09, 12 April 2017
Line 1:		Line 1:
		+
	==Crystal structure of the ectodomain of African green monkey CD81 large extracellular loop (agmCD81-LEL)==		==Crystal structure of the ectodomain of African green monkey CD81 large extracellular loop (agmCD81-LEL)==
	<StructureSection load='3x0g' size='340' side='right' caption='[[3x0g]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3x0g' size='340' side='right' caption='[[3x0g]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g8q\|1g8q]], [[1iv5\|1iv5]], [[3x0e\|3x0e]], [[3x0f\|3x0f]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g8q\|1g8q]], [[1iv5\|1iv5]], [[3x0e\|3x0e]], [[3x0f\|3x0f]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [http://www.ebi.ac.uk/pdbsum/3x0g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [http://pdbe.org/3x0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [http://www.ebi.ac.uk/pdbsum/3x0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0g ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3x0g" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:43, 8 July 2015

2015-07-08T08:43:26Z

←Older revision		Revision as of 08:43, 8 July 2015
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [http://www.ebi.ac.uk/pdbsum/3x0g PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [http://www.ebi.ac.uk/pdbsum/3x0g PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hepatitis C virus (HCV) infection is one of the leading causes of chronic liver diseases; however, HCV vaccine remains unavailable to date. One main obstacle is the lack of an efficient small animal model. Cluster of differentiation 81 (CD81) is an essential entry coreceptor for HCV species specificity to human, though the underlying mechanisms are yet to be fully elucidated. We performed structural, biophysical, and virologic studies on HCV nonpermissive CD81s from mouse and African green monkey [mouse cluster of differentiation 81 (mCD81) and African green monkey cluster of differentiation 81 (agmCD81)] and compared with human cluster of differentiation 81 (hCD81). We discovered an intramolecular hydrogen bond (Gln188-Nepsilon2-H: Glu196-Oepsilon2, 2 A, 124 degrees ) within the large extracellular loop (LEL) of mCD81 and a salt bridge (Lys188-Nzeta: Asp196-Odelta2, 2.4 A) within agmCD81-LEL between 188-196 residues. This structural feature is missing in hCD81. We demonstrated that the introduction of a single 188-196 bond to hCD81 impaired its binding affinity to HCV envelope glycoprotein 2 (HCV E2) and significantly decreased HCV pseudoviral particle (HCVpp) entry efficiency (4.92- to 8.42-fold) and cell culture-grown HCV (HCVcc) infectivity (4.55-fold), despite the availability of Phe186. For HCV nonpermissive CD81s, the introduction of Phe186 by Leu186F substitution alone was insufficient to confer HCV permissiveness. The disruption of the original 188-196 bond and Leu186F substitution were both required for potent binding to HCV E2 HCVpp entry efficiency and HCVcc infectivity. Our structural and biophysical analyses suggest that the intramolecular 188-196 bond restricts the intrinsic conformational dynamics of D-helix of CD81-LEL, which is essential for HCV entry, thus impairs HCV permissiveness. Our findings reveal a novel molecular determinant for HCV entry in addition to the well-characterized Phe186 and provide further guideline for selecting an HCV small animal model.-Yang, W., Zhang, M., Chi, X., Liu, X., Qin, B., Cui, S. An intramolecular bond at cluster of differentiation 81 ectodomain is important for hepatitis C virus entry.
		+
		+	An intramolecular bond at cluster of differentiation 81 ectodomain is important for hepatitis C virus entry.,Yang W, Zhang M, Chi X, Liu X, Qin B, Cui S FASEB J. 2015 Jun 26. pii: fj.15-272880. PMID:26116703<ref>PMID:26116703</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:16, 1 July 2015

2015-07-01T12:16:40Z

←Older revision		Revision as of 12:16, 1 July 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of the ectodomain of African green monkey CD81 large extracellular loop (agmCD81-LEL)==
-		+	<StructureSection load='3x0g' size='340' side='right' caption='[[3x0g]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~The entry~~ 3x0g is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3x0g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X0G FirstGlance]. <br>
-	~~Authors~~: ~~Zhang, M~~.~~, Cui, S~~.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g8q\|1g8q]], [[1iv5\|1iv5]], [[3x0e\|3x0e]], [[3x0f\|3x0f]]</td></tr>
-	~~Description~~: ~~Structure of the ectodomain of a tetraspanin~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3x0g RCSB], [http://www.ebi.ac.uk/pdbsum/3x0g PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Zhang~~, M]]	+	__TOC__
		+	</StructureSection>
	[[Category: Cui, S]]		[[Category: Cui, S]]
		+	[[Category: Zhang, M]]
		+	[[Category: Activation]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Differentiation]]
		+	[[Category: Disulfide bond]]
		+	[[Category: Helical bundle]]
		+	[[Category: Immune cell adhesion]]
		+	[[Category: Membrane]]
		+	[[Category: Morphology]]
		+	[[Category: Proliferation]]

OCA at 13:11, 24 December 2014

2014-12-24T13:11:14Z

←Older revision		Revision as of 13:11, 24 December 2014
Line 6:		Line 6:

	Description: Structure of the ectodomain of a tetraspanin		Description: Structure of the ectodomain of a tetraspanin
		+	[[Category: Unreleased Structures]]
		+	[[Category: Zhang, M]]
		+	[[Category: Cui, S]]

OCA at 11:03, 19 November 2014

2014-11-19T11:03:25Z

←Older revision		Revision as of 11:03, 19 November 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3x0g is ON HOLD	+	The entry 3x0g is ON HOLD until Paper Publication

	Authors: Zhang, M., Cui, S.		Authors: Zhang, M., Cui, S.

	Description: Structure of the ectodomain of a tetraspanin		Description: Structure of the ectodomain of a tetraspanin