3ze4 - Revision history

OCA at 07:47, 7 February 2024

2024-02-07T07:47:12Z

←Older revision		Revision as of 07:47, 7 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ze4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ze4 OCA], [https://pdbe.org/3ze4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ze4 RCSB], [https://www.ebi.ac.uk/pdbsum/3ze4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ze4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.702Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ze4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ze4 OCA], [https://pdbe.org/3ze4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ze4 RCSB], [https://www.ebi.ac.uk/pdbsum/3ze4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ze4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/KDGL_ECOLI KDGL_ECOLI] Recycling of diacylglycerol produced during the turnover of membrane phospholipid.		[https://www.uniprot.org/uniprot/KDGL_ECOLI KDGL_ECOLI] Recycling of diacylglycerol produced during the turnover of membrane phospholipid.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Diacylglycerol kinase catalyses the ATP-dependent phosphorylation of diacylglycerol to phosphatidic acid for use in shuttling water-soluble components to membrane-derived oligosaccharide and lipopolysaccharide in the cell envelope of Gram-negative bacteria. For half a century, this 121-residue kinase has served as a model for investigating membrane protein enzymology, folding, assembly and stability. Here we present crystal structures for three functional forms of this unique and paradigmatic kinase, one of which is wild type. These reveal a homo-trimeric enzyme with three transmembrane helices and an amino-terminal amphiphilic helix per monomer. Bound lipid substrate and docked ATP identify the putative active site that is of the composite, shared site type. The crystal structures rationalize extensive biochemical and biophysical data on the enzyme. They are, however, at variance with a published solution NMR model in that domain swapping, a key feature of the solution form, is not observed in the crystal structures.
-
-	Crystal structure of the integral membrane diacylglycerol kinase.,Li D, Lyons JA, Pye VE, Vogeley L, Aragao D, Kenyon CP, Shah ST, Doherty C, Aherne M, Caffrey M Nature. 2013 May 23;497(7450):521-4. doi: 10.1038/nature12179. Epub 2013 May 15. PMID:23676677<ref>PMID:23676677</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3ze4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Diacylglycerol kinase 3D structures\|Diacylglycerol kinase 3D structures]]		*[[Diacylglycerol kinase 3D structures\|Diacylglycerol kinase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:56, 29 March 2023

2023-03-29T06:56:14Z

←Older revision		Revision as of 06:56, 29 March 2023
Line 3:		Line 3:
	<StructureSection load='3ze4' size='340' side='right'caption='[[3ze4]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='3ze4' size='340' side='right'caption='[[3ze4]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ze3\|3ze3]], [[3ze5\|3ze5]]</div></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ze4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ze4 OCA], [https://pdbe.org/3ze4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ze4 RCSB], [https://www.ebi.ac.uk/pdbsum/3ze4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ze4 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Diacylglycerol_kinase Diacylglycerol kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.107 2.7.1.107] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ze4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ze4 OCA], [https://pdbe.org/3ze4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ze4 RCSB], [https://www.ebi.ac.uk/pdbsum/3ze4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ze4 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/KDGL_ECOLI KDGL_ECOLI]] Recycling of diacylglycerol produced during the turnover of membrane phospholipid.	+	[https://www.uniprot.org/uniprot/KDGL_ECOLI KDGL_ECOLI] Recycling of diacylglycerol produced during the turnover of membrane phospholipid.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Diacylglycerol kinase]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aragao, D]]	+	[[Category: Aragao D]]
-	[[Category: Caffrey, M]]	+	[[Category: Caffrey M]]
-	[[Category: Li, D]]	+	[[Category: Li D]]
-	[[Category: Lyons~~, J A~~]]	+	[[Category: Lyons JA]]
-	[[Category: Pye~~, V E~~]]	+	[[Category: Pye VE]]
-	[[Category: Vogeley, L]]	+	[[Category: Vogeley L]]
-	~~[[Category: 8 mag]]~~	+
-	~~[[Category: In meso crystallisation]]~~	+
-	~~[[Category: Lipid cubic phase]]~~	+
-	~~[[Category: Lipid metabolism]]~~	+
-	~~[[Category: Lipidic mesophase]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Monoacylglycerol]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 05:43, 10 August 2022

2022-08-10T05:43:44Z

←Older revision		Revision as of 05:43, 10 August 2022
Line 3:		Line 3:
	<StructureSection load='3ze4' size='340' side='right'caption='[[3ze4]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='3ze4' size='340' side='right'caption='[[3ze4]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ze3\|3ze3]], [[3ze5\|3ze5]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ze3\|3ze3]], [[3ze5\|3ze5]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Diacylglycerol_kinase Diacylglycerol kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.107 2.7.1.107] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Diacylglycerol_kinase Diacylglycerol kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.107 2.7.1.107] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ze4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ze4 OCA], [~~http~~://pdbe.org/3ze4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ze4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ze4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ze4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ze4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ze4 OCA], [https://pdbe.org/3ze4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ze4 RCSB], [https://www.ebi.ac.uk/pdbsum/3ze4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ze4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/KDGL_ECOLI KDGL_ECOLI]] Recycling of diacylglycerol produced during the turnover of membrane phospholipid.	+	[[https://www.uniprot.org/uniprot/KDGL_ECOLI KDGL_ECOLI]] Recycling of diacylglycerol produced during the turnover of membrane phospholipid.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Diacylglycerol kinase\|Diacylglycerol kinase]]	+	*[[Diacylglycerol kinase 3D structures\|Diacylglycerol kinase 3D structures]]
-	*[[NMR Ensembles of Models\|NMR Ensembles of Models]]	+
-	*[[Quality assessment for molecular models\|Quality assessment for molecular models]]	+
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:39, 3 April 2019

2019-04-03T07:39:48Z

←Older revision		Revision as of 07:39, 3 April 2019
Line 1:		Line 1:

	==Crystal structure of the integral membrane diacylglycerol kinase - wild-type==		==Crystal structure of the integral membrane diacylglycerol kinase - wild-type==
-	<StructureSection load='3ze4' size='340' side='right' caption='[[3ze4]], [[Resolution\|resolution]] 3.70Å' scene=''>	+	<StructureSection load='3ze4' size='340' side='right'caption='[[3ze4]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>
Line 30:		Line 30:
	[[Category: Diacylglycerol kinase]]		[[Category: Diacylglycerol kinase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Aragao, D]]		[[Category: Aragao, D]]
	[[Category: Caffrey, M]]		[[Category: Caffrey, M]]

OCA at 08:01, 6 March 2019

2019-03-06T08:01:02Z

←Older revision		Revision as of 08:01, 6 March 2019
Line 1:		Line 1:

	==Crystal structure of the integral membrane diacylglycerol kinase - wild-type==		==Crystal structure of the integral membrane diacylglycerol kinase - wild-type==
-	<StructureSection load='3ze4' size='340' side='right' caption='[[3ze4]], [[Resolution\|resolution]] 3.70Å' scene=''>	+	<StructureSection load='3ze4' size='340' side='right' caption='[[3ze4]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>

OCA at 22:31, 4 August 2016

2016-08-04T22:31:57Z

←Older revision		Revision as of 22:31, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the integral membrane diacylglycerol kinase - wild-type==		==Crystal structure of the integral membrane diacylglycerol kinase - wild-type==
	<StructureSection load='3ze4' size='340' side='right' caption='[[3ze4]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='3ze4' size='340' side='right' caption='[[3ze4]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ze3\|3ze3]], [[3ze5\|3ze5]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ze3\|3ze3]], [[3ze5\|3ze5]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diacylglycerol_kinase Diacylglycerol kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.107 2.7.1.107] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diacylglycerol_kinase Diacylglycerol kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.107 2.7.1.107] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ze4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ze4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ze4 RCSB], [http://www.ebi.ac.uk/pdbsum/3ze4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ze4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ze4 OCA], [http://pdbe.org/3ze4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ze4 RCSB], [http://www.ebi.ac.uk/pdbsum/3ze4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ze4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ze4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Diacylglycerol kinase\|Diacylglycerol kinase]]
		+	*[[NMR Ensembles of Models\|NMR Ensembles of Models]]
		+	*[[Quality assessment for molecular models\|Quality assessment for molecular models]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Diacylglycerol kinase]]		[[Category: Diacylglycerol kinase]]
-	[[Category: ~~Escherichia coli k-12~~]]	+	[[Category: Ecoli]]
	[[Category: Aragao, D]]		[[Category: Aragao, D]]
	[[Category: Caffrey, M]]		[[Category: Caffrey, M]]

OCA at 20:15, 24 December 2014

2014-12-24T20:15:09Z

←Older revision		Revision as of 20:15, 24 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3ze4\| PDB=3ze4 \| SCENE= }}~~	+	==Crystal structure of the integral membrane diacylglycerol kinase - wild-type==
-	===Crystal structure of the integral membrane diacylglycerol kinase - wild-type===	+	<StructureSection load='3ze4' size='340' side='right' caption='[[3ze4]], [[Resolution\|resolution]] 3.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23676677}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3ze4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZE4 FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ze3\|3ze3]], [[3ze5\|3ze5]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diacylglycerol_kinase Diacylglycerol kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.107 2.7.1.107] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ze4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ze4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ze4 RCSB], [http://www.ebi.ac.uk/pdbsum/3ze4 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/KDGL_ECOLI KDGL_ECOLI]] Recycling of diacylglycerol produced during the turnover of membrane phospholipid.		[[http://www.uniprot.org/uniprot/KDGL_ECOLI KDGL_ECOLI]] Recycling of diacylglycerol produced during the turnover of membrane phospholipid.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Diacylglycerol kinase catalyses the ATP-dependent phosphorylation of diacylglycerol to phosphatidic acid for use in shuttling water-soluble components to membrane-derived oligosaccharide and lipopolysaccharide in the cell envelope of Gram-negative bacteria. For half a century, this 121-residue kinase has served as a model for investigating membrane protein enzymology, folding, assembly and stability. Here we present crystal structures for three functional forms of this unique and paradigmatic kinase, one of which is wild type. These reveal a homo-trimeric enzyme with three transmembrane helices and an amino-terminal amphiphilic helix per monomer. Bound lipid substrate and docked ATP identify the putative active site that is of the composite, shared site type. The crystal structures rationalize extensive biochemical and biophysical data on the enzyme. They are, however, at variance with a published solution NMR model in that domain swapping, a key feature of the solution form, is not observed in the crystal structures.

-	~~==About this Structure==~~	+	Crystal structure of the integral membrane diacylglycerol kinase.,Li D, Lyons JA, Pye VE, Vogeley L, Aragao D, Kenyon CP, Shah ST, Doherty C, Aherne M, Caffrey M Nature. 2013 May 23;497(7450):521-4. doi: 10.1038/nature12179. Epub 2013 May 15. PMID:23676677<ref>PMID:23676677</ref>
-	~~[[3ze4]] is a 3 chain~~ structure ~~with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli_k~~-~~12 Escherichia coli k-12]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3ZE4 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023676677</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Diacylglycerol kinase]]		[[Category: Diacylglycerol kinase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
-	[[Category: Aragao, D.]]	+	[[Category: Aragao, D]]
-	[[Category: Caffrey, M.]]	+	[[Category: Caffrey, M]]
-	[[Category: Li, D.]]	+	[[Category: Li, D]]
-	[[Category: Lyons, J A.]]	+	[[Category: Lyons, J A]]
-	[[Category: Pye, V E.]]	+	[[Category: Pye, V E]]
-	[[Category: Vogeley, L.]]	+	[[Category: Vogeley, L]]
-	[[Category: 7 8 mag]]	+	[[Category: 8 mag]]
	[[Category: In meso crystallisation]]		[[Category: In meso crystallisation]]
	[[Category: Lipid cubic phase]]		[[Category: Lipid cubic phase]]

OCA at 15:05, 19 June 2013

2013-06-19T15:05:07Z

←Older revision		Revision as of 15:05, 19 June 2013
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-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_3ze4\| PDB=3ze4 \| SCENE= }}
		+	===Crystal structure of the integral membrane diacylglycerol kinase - wild-type===
		+	{{ABSTRACT_PUBMED_23676677}}

-	~~The entry 3ze4 is ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/KDGL_ECOLI KDGL_ECOLI]] Recycling of diacylglycerol produced during the turnover of membrane phospholipid.

-	~~Authors~~: ~~Li, D~~.~~, Lyons, J~~.A.~~, Pye, V.E~~.~~, Vogeley, L~~.~~, Aragao, D~~.~~, Caffrey, M~~.	+	==About this Structure==
		+	[[3ze4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZE4 OCA].

-	~~Description~~: ~~Crystal structure of the integral membrane diacylglycerol~~ kinase -~~wild-type~~	+	==Reference==
		+	<ref group="xtra">PMID:023676677</ref><references group="xtra"/><references/>
		+	[[Category: Diacylglycerol kinase]]
		+	[[Category: Escherichia coli k-12]]
		+	[[Category: Aragao, D.]]
		+	[[Category: Caffrey, M.]]
		+	[[Category: Li, D.]]
		+	[[Category: Lyons, J A.]]
		+	[[Category: Pye, V E.]]
		+	[[Category: Vogeley, L.]]
		+	[[Category: 7 8 mag]]
		+	[[Category: In meso crystallisation]]
		+	[[Category: Lipid cubic phase]]
		+	[[Category: Lipid metabolism]]
		+	[[Category: Lipidic mesophase]]
		+	[[Category: Membrane protein]]
		+	[[Category: Monoacylglycerol]]
		+	[[Category: Transferase]]

OCA at 10:48, 19 December 2012

2012-12-19T10:48:36Z

←Older revision		Revision as of 10:48, 19 December 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3ze4 is ON HOLD	+	The entry 3ze4 is ON HOLD until Paper Publication

	Authors: Li, D., Lyons, J.A., Pye, V.E., Vogeley, L., Aragao, D., Caffrey, M.		Authors: Li, D., Lyons, J.A., Pye, V.E., Vogeley, L., Aragao, D., Caffrey, M.

	Description: Crystal structure of the integral membrane diacylglycerol kinase -wild-type		Description: Crystal structure of the integral membrane diacylglycerol kinase -wild-type

OCA: Protected "3ze4" [edit=sysop:move=sysop]

2012-12-12T20:35:27Z

Protected "3ze4" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:35, 12 December 2012