3zfe - Revision history

OCA at 11:00, 20 December 2023

2023-12-20T11:00:44Z

←Older revision		Revision as of 11:00, 20 December 2023
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	<StructureSection load='3zfe' size='340' side='right'caption='[[3zfe]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3zfe' size='340' side='right'caption='[[3zfe]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zfe]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human_enterovirus_71 Human enterovirus 71~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZFE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zfe]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterovirus_A71 Enterovirus A71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZFE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPH:SPHINGOSINE'>SPH</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zff\|3zff]], [[3zfg\|3zfg]]</div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPH:SPHINGOSINE'>SPH</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfe OCA], [https://pdbe.org/3zfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zfe RCSB], [https://www.ebi.ac.uk/pdbsum/3zfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfe ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfe OCA], [https://pdbe.org/3zfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zfe RCSB], [https://www.ebi.ac.uk/pdbsum/3zfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~A9X4C2_9ENTO A9X4C2_9ENTO~~]] ~~Protein 2C associates~~ with and induces ~~structural rearrangements~~ of ~~intracellular membranes~~. ~~It displays~~ RNA~~-binding~~, ~~nucleotide~~ binding and ~~NTPase activities~~ (By similarity).[~~SAAS~~:~~SAAS000199_004_016611~~] ~~Protein 3C~~ is a ~~cysteine~~ protease that ~~generates mature~~ viral proteins ~~from~~ the ~~precursor~~ polyprotein. ~~In addition~~ to ~~its proteolytic activity~~, ~~it binds~~ to ~~viral RNA~~, and ~~thus influences~~ viral genome replication. RNA and ~~substrate bind co~~-~~operatively~~ to the ~~protease~~ (~~By similarity~~).[~~SAAS~~:~~SAAS000199_004_042266~~] RNA-directed RNA polymerase 3D-~~POL replicates~~ genomic and ~~antigenomic~~ RNA ~~by recognizing replications specific signals~~ (~~By similarity~~).[~~SAAS~~:~~SAAS000199_004_010047~~]	+	[https://www.uniprot.org/uniprot/A9X4C2_HE71 A9X4C2_HE71] Acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU (By similarity). The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome (By similarity). Following genome release from the infecting virion in the cytoplasm, the VPg-RNA linkage is probably removed by host TDP2 (By similarity). During the late stage of the replication cycle, host TDP2 is excluded from sites of viral RNA synthesis and encapsidation, allowing for the generation of progeny virions.[ARBA:ARBA00024846] Capsid protein VP0: Component of immature procapsids, which is cleaved into capsid proteins VP4 and VP2 after maturation. Allows the capsid to remain inactive before the maturation step.[RuleBase:RU364118] Capsid protein VP1: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor to provide virion attachment to target host cells. This attachment induces virion internalization. Tyrosine kinases are probably involved in the entry process. After binding to its receptor, the capsid undergoes conformational changes. Capsid protein VP1 N-terminus (that contains an amphipathic alpha-helix) and capsid protein VP4 are externalized. Together, they shape a pore in the host membrane through which viral genome is translocated to host cell cytoplasm. After genome has been released, the channel shrinks.[RuleBase:RU364118] Capsid protein VP2: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome.[RuleBase:RU364118] Capsid protein VP3: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome.[RuleBase:RU364118] Capsid protein VP4: Lies on the inner surface of the capsid shell. After binding to the host receptor, the capsid undergoes conformational changes. Capsid protein VP4 is released, Capsid protein VP1 N-terminus is externalized, and together, they shape a pore in the host membrane through which the viral genome is translocated into the host cell cytoplasm.[RuleBase:RU364118] Component of immature procapsids, which is cleaved into capsid proteins VP4 and VP2 after maturation (By similarity). Allows the capsid to remain inactive before the maturation step.[ARBA:ARBA00025202] Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3 (By similarity). The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome.[ARBA:ARBA00025306] Lies on the inner surface of the capsid shell (By similarity). After binding to the host receptor, the capsid undergoes conformational changes (By similarity). Capsid protein VP4 is released, Capsid protein VP1 N-terminus is externalized, and together, they shape a pore in the host membrane through which the viral genome is translocated into the host cell cytoplasm.[ARBA:ARBA00024643] Protease 2A: Cysteine protease that cleaves viral polyprotein and specific host proteins.[RuleBase:RU364118] Protease 3C: Major viral protease that mediates proteolytic processing of the polyprotein. Cleaves host EIF5B, contributing to host translation shutoff. Cleaves also host PABPC1, contributing to host translation shutoff.[RuleBase:RU364118] Protein 2B: Plays an essential role in the virus replication cycle by acting as a viroporin. Creates a pore in the host reticulum endoplasmic and as a consequence releases Ca2+ in the cytoplasm of infected cell. In turn, high levels of cytoplasmic calcium may trigger membrane trafficking and transport of viral ER-associated proteins to viroplasms, sites of viral genome replication.[RuleBase:RU364118] Protein 2C: Induces and associates with structural rearrangements of intracellular membranes. Displays RNA-binding, nucleotide binding and NTPase activities. May play a role in virion morphogenesis and viral RNA encapsidation by interacting with the capsid protein VP3.[RuleBase:RU364118] Protein 3A: Localizes the viral replication complex to the surface of membranous vesicles. It inhibits host cell endoplasmic reticulum-to-Golgi apparatus transport and causes the disassembly of the Golgi complex, possibly through GBF1 interaction. This would result in depletion of MHC, trail receptors and IFN receptors at the host cell surface.[RuleBase:RU364118] Protein 3AB: Localizes the viral replication complex to the surface of membranous vesicles. Together with protein 3CD binds the Cis-Active RNA Element (CRE) which is involved in RNA synthesis initiation. Acts as a cofactor to stimulate the activity of 3D polymerase, maybe through a nucleid acid chaperone activity.[RuleBase:RU364118] Protein 3CD: Involved in the viral replication complex and viral polypeptide maturation. It exhibits protease activity with a specificity and catalytic efficiency that is different from protease 3C. Protein 3CD lacks polymerase activity. Protein 3CD binds to the 5'UTR of the viral genome.[RuleBase:RU364118] RNA-directed RNA polymerase: Replicates the viral genomic RNA on the surface of intracellular membranes. May form linear arrays of subunits that propagate along a strong head-to-tail interaction called interface-I. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form. This dsRNA is then used as template to synthesize positive stranded RNA genomes. ss(+)RNA genomes are either translated, replicated or encapsidated.[RuleBase:RU364118] Viral protein genome-linked: acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU. The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome.[RuleBase:RU364118]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human enterovirus 71~~]]	+	[[Category: Enterovirus A71]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cardosa, J]]	+	[[Category: Cardosa J]]
-	[[Category: Kuhn~~, R J~~]]	+	[[Category: Kuhn RJ]]
-	[[Category: Perera, R]]	+	[[Category: Perera R]]
-	[[Category: Plevka, P]]	+	[[Category: Plevka P]]
-	[[Category: Rossmann~~, M G~~]]	+	[[Category: Rossmann MG]]
-	[[Category: Yap~~, M L]]~~	+	[[Category: Yap ML]]
-	~~[[Category: Ev71]]~~	+
-	~~[[Category: Picornavirus]]~~	+
-	~~[[Category: Virus~~]]	+

OCA at 05:44, 10 August 2022

2022-08-10T05:44:47Z

←Older revision		Revision as of 05:44, 10 August 2022
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	==Human enterovirus 71 in complex with capsid binding inhibitor WIN51711==		==Human enterovirus 71 in complex with capsid binding inhibitor WIN51711==
-	<StructureSection load='3zfe' size='340' side='right' caption='[[3zfe]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='3zfe' size='340' side='right'caption='[[3zfe]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zfe]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human_enterovirus_71 Human enterovirus 71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZFE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zfe]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_enterovirus_71 Human enterovirus 71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZFE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPH:SPHINGOSINE'>SPH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPH:SPHINGOSINE'>SPH</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zff\|3zff]], [[3zfg\|3zfg]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3zff\|3zff]], [[3zfg\|3zfg]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfe OCA], [~~http~~://pdbe.org/3zfe PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zfe RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zfe PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfe ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfe OCA], [https://pdbe.org/3zfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zfe RCSB], [https://www.ebi.ac.uk/pdbsum/3zfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/A9X4C2_9ENTO A9X4C2_9ENTO]] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]	+	[[https://www.uniprot.org/uniprot/A9X4C2_9ENTO A9X4C2_9ENTO]] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 3zfe" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3zfe" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Virus coat proteins 3D structures\|Virus coat proteins 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Human enterovirus 71]]		[[Category: Human enterovirus 71]]
		+	[[Category: Large Structures]]
	[[Category: Cardosa, J]]		[[Category: Cardosa, J]]
	[[Category: Kuhn, R J]]		[[Category: Kuhn, R J]]

OCA at 16:42, 27 February 2019

2019-02-27T16:42:09Z

←Older revision		Revision as of 16:42, 27 February 2019
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	<StructureSection load='3zfe' size='340' side='right' caption='[[3zfe]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3zfe' size='340' side='right' caption='[[3zfe]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zfe]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Ev-71 Ev-~~71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZFE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zfe]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_enterovirus_71 Human enterovirus 71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZFE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPH:SPHINGOSINE'>SPH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPH:SPHINGOSINE'>SPH</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zff\|3zff]], [[3zfg\|3zfg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zff\|3zff]], [[3zfg\|3zfg]]</td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ev-~~71]]	+	[[Category: Human enterovirus 71]]
	[[Category: Cardosa, J]]		[[Category: Cardosa, J]]
	[[Category: Kuhn, R J]]		[[Category: Kuhn, R J]]

OCA at 00:30, 5 August 2016

2016-08-05T00:30:16Z

←Older revision		Revision as of 00:30, 5 August 2016
Line 1:		Line 1:
		+
	==Human enterovirus 71 in complex with capsid binding inhibitor WIN51711==		==Human enterovirus 71 in complex with capsid binding inhibitor WIN51711==
	<StructureSection load='3zfe' size='340' side='right' caption='[[3zfe]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3zfe' size='340' side='right' caption='[[3zfe]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPH:SPHINGOSINE'>SPH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPH:SPHINGOSINE'>SPH</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zff\|3zff]], [[3zfg\|3zfg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zff\|3zff]], [[3zfg\|3zfg]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zfe RCSB], [http://www.ebi.ac.uk/pdbsum/3zfe PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfe OCA], [http://pdbe.org/3zfe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zfe RCSB], [http://www.ebi.ac.uk/pdbsum/3zfe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zfe" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:11, 24 December 2014

2014-12-24T21:11:50Z

←Older revision		Revision as of 21:11, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zfe RCSB], [http://www.ebi.ac.uk/pdbsum/3zfe PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zfe RCSB], [http://www.ebi.ac.uk/pdbsum/3zfe PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A9X4C2_9ENTO A9X4C2_9ENTO]] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:31, 21 December 2014

2014-12-21T09:31:47Z

←Older revision		Revision as of 09:31, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3zfe~~\| ~~PDB~~=3zfe \| ~~SCENE~~= }}	+	==Human enterovirus 71 in complex with capsid binding inhibitor WIN51711==
-	===Human enterovirus 71 in ~~complex~~ with capsid binding inhibitor ~~WIN51711===~~	+	<StructureSection load='3zfe' size='340' side='right' caption='[[3zfe]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23509286}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zfe]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ev-71 Ev-71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZFE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPH:SPHINGOSINE'>SPH</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3zff\|3zff]], [[3zfg\|3zfg]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zfe RCSB], [http://www.ebi.ac.uk/pdbsum/3zfe PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human enterovirus 71 is a picornavirus causing hand, foot, and mouth disease that may progress to fatal encephalitis in infants and small children. As of now, no cure is available for enterovirus 71 infections. Small molecule inhibitors binding into a hydrophobic pocket within capsid viral protein 1 were previously shown to effectively limit infectivity of many picornaviruses. Here we report a 3.2-A-resolution X-ray structure of the enterovirus 71 virion complexed with the capsid-binding inhibitor WIN 51711. The inhibitor replaced the natural pocket factor within the viral protein 1 pocket without inducing any detectable rearrangements in the structure of the capsid. Furthermore, we show that the compound stabilizes enterovirus 71 virions and limits its infectivity, probably through restricting dynamics of the capsid necessary for genome release. Thus, our results provide a structural basis for development of antienterovirus 71 capsid-binding drugs.

-	~~==Function==~~	+	Structure of human enterovirus 71 in complex with a capsid-binding inhibitor.,Plevka P, Perera R, Yap ML, Cardosa J, Kuhn RJ, Rossmann MG Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5463-7. doi:, 10.1073/pnas.1222379110. Epub 2013 Mar 18. PMID:23509286<ref>PMID:23509286</ref>
-	~~[[http://www.uniprot.org/uniprot/A9X4C2_9ENTO A9X4C2_9ENTO]] Protein 2C associates~~ with ~~and induces structural rearrangements of intracellular membranes. It displays RNA~~-binding~~, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein~~. ~~In addition to its proteolytic activity~~, ~~it binds to viral RNA~~, ~~and thus influences viral genome replication~~. ~~RNA and substrate bind co-operatively to the protease~~ (~~By similarity~~)~~.[SAAS~~:~~SAAS000199_004_042266] RNA~~-~~directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity)~~.~~[SAAS~~:~~SAAS000199_004_010047]~~	+

-	==~~About this Structure~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3zfe]] is a 4 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Human_enterovirus_71 Human enterovirus 71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFE OCA].~~	+	</div>
-	[[Category: ~~Human enterovirus~~ 71]]	+	== References ==
-	[[Category: Cardosa, J.]]	+	<references/>
-	[[Category: Kuhn, R J.]]	+	__TOC__
-	[[Category: Perera, R.]]	+	</StructureSection>
-	[[Category: Plevka, P.]]	+	[[Category: Ev-71]]
-	[[Category: Rossmann, M G.]]	+	[[Category: Cardosa, J]]
-	[[Category: Yap, M L.]]	+	[[Category: Kuhn, R J]]
		+	[[Category: Perera, R]]
		+	[[Category: Plevka, P]]
		+	[[Category: Rossmann, M G]]
		+	[[Category: Yap, M L]]
	[[Category: Ev71]]		[[Category: Ev71]]
	[[Category: Picornavirus]]		[[Category: Picornavirus]]
	[[Category: Virus]]		[[Category: Virus]]

OCA at 22:00, 10 April 2013

2013-04-10T22:00:20Z

←Older revision		Revision as of 22:00, 10 April 2013
Line 1:		Line 1:
	{{STRUCTURE_3zfe\| PDB=3zfe \| SCENE= }}		{{STRUCTURE_3zfe\| PDB=3zfe \| SCENE= }}
	===Human enterovirus 71 in complex with capsid binding inhibitor WIN51711===		===Human enterovirus 71 in complex with capsid binding inhibitor WIN51711===
		+	{{ABSTRACT_PUBMED_23509286}}

	==Function==		==Function==

OCA at 08:53, 27 March 2013

2013-03-27T08:53:49Z

←Older revision		Revision as of 08:53, 27 March 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_3zfe\| PDB=3zfe \| SCENE= }}
		+	===Human enterovirus 71 in complex with capsid binding inhibitor WIN51711===

-	~~The entry 3zfe~~ is ~~ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/A9X4C2_9ENTO A9X4C2_9ENTO]] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]

-	~~Authors~~: ~~Plevka, P~~.~~, Perera, R~~.~~, Yap, M~~.L., Cardosa, J., Kuhn, R.J., Rossmann, M.G.	+	==About this Structure==
-		+	[[3zfe]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_enterovirus_71 Human enterovirus 71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFE OCA].
-	~~Description~~: ~~Human enterovirus 71 in complex with capsid binding inhibitor WIN51711~~	+	[[Category: Human enterovirus 71]]
		+	[[Category: Cardosa, J.]]
		+	[[Category: Kuhn, R J.]]
		+	[[Category: Perera, R.]]
		+	[[Category: Plevka, P.]]
		+	[[Category: Rossmann, M G.]]
		+	[[Category: Yap, M L.]]
		+	[[Category: Ev71]]
		+	[[Category: Picornavirus]]
		+	[[Category: Virus]]

OCA: Protected "3zfe" [edit=sysop:move=sysop]

2012-12-19T10:49:30Z

Protected "3zfe" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:49, 19 December 2012

OCA: New page: '''Unreleased structure''' The entry 3zfe is ON HOLD Authors: Plevka, P., Perera, R., Yap, M.L., Cardosa, J., Kuhn, R.J., Rossmann, M.G. Description: Human enterovirus 71 in complex wi...

2012-12-19T10:49:29Z

New page: '''Unreleased structure''' The entry 3zfe is ON HOLD Authors: Plevka, P., Perera, R., Yap, M.L., Cardosa, J., Kuhn, R.J., Rossmann, M.G. Description: Human enterovirus 71 in complex wi...

New page

'''Unreleased structure'''

The entry 3zfe is ON HOLD

Authors: Plevka, P., Perera, R., Yap, M.L., Cardosa, J., Kuhn, R.J., Rossmann, M.G.

Description: Human enterovirus 71 in complex with capsid binding inhibitor WIN51711