3zzj - Revision history

OCA at 11:16, 20 December 2023

2023-12-20T11:16:39Z

←Older revision		Revision as of 11:16, 20 December 2023
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	<StructureSection load='3zzj' size='340' side='right'caption='[[3zzj]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3zzj' size='340' side='right'caption='[[3zzj]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zzj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZZJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zzj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZZJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1arg\|1arg]], [[1aam\|1aam]], [[1toe\|1toe]], [[1ix7\|1ix7]], [[1cq6\|1cq6]], [[1asn\|1asn]], [[1g7x\|1g7x]], [[1ahx\|1ahx]], [[1asg\|1asg]], [[1ahy\|1ahy]], [[1qis\|1qis]], [[1g7w\|1g7w]], [[1qir\|1qir]], [[1ahe\|1ahe]], [[1ase\|1ase]], [[1ams\|1ams]], [[2aat\|2aat]], [[1arh\|1arh]], [[1ari\|1ari]], [[1ix8\|1ix8]], [[1bqd\|1bqd]], [[1cq8\|1cq8]], [[1asb\|1asb]], [[1asd\|1asd]], [[1asa\|1asa]], [[1ahf\|1ahf]], [[1art\|1art]], [[1toi\|1toi]], [[5eaa\|5eaa]], [[1czc\|1czc]], [[1aib\|1aib]], [[1ix6\|1ix6]], [[1asm\|1asm]], [[1yoo\|1yoo]], [[1asf\|1asf]], [[1g4x\|1g4x]], [[1c9c\|1c9c]], [[1b4x\|1b4x]], [[1bqa\|1bqa]], [[1spa\|1spa]], [[1tok\|1tok]], [[1toj\|1toj]], [[1aic\|1aic]], [[1cq7\|1cq7]], [[1aaw\|1aaw]], [[1ars\|1ars]], [[1g4v\|1g4v]], [[1aia\|1aia]], [[1asl\|1asl]], [[3aat\|3aat]], [[1asc\|1asc]], [[1tog\|1tog]], [[1amr\|1amr]], [[1cze\|1cze]], [[1qit\|1qit]], [[1ahg\|1ahg]], [[1amq\|1amq]], [[3zzk\|3zzk]], [[4a00\|4a00]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzj OCA], [https://pdbe.org/3zzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zzj RCSB], [https://www.ebi.ac.uk/pdbsum/3zzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzj OCA], [https://pdbe.org/3zzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zzj RCSB], [https://www.ebi.ac.uk/pdbsum/3zzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AAT_ECOLI AAT_ECOLI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Aspartate transaminase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Christen, P]]	+	[[Category: Christen P]]
-	[[Category: Coll, M]]	+	[[Category: Coll M]]
-	[[Category: Fernandez~~, F J~~]]	+	[[Category: Fernandez FJ]]
-	[[Category: Gehring, H]]	+	[[Category: Gehring H]]
-	[[Category: Pena-Soler, E]]	+	[[Category: Pena-Soler E]]
-	[[Category: Vega~~, M C~~]]	+	[[Category: Vega MC]]
-	[[Category: DeVries, D]]	+	[[Category: DeVries D]]
-	~~[[Category: Transferase~~]]	+

OCA at 07:30, 18 August 2022

2022-08-18T07:30:21Z

←Older revision		Revision as of 07:30, 18 August 2022
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	==Structure of an engineered aspartate aminotransferase==		==Structure of an engineered aspartate aminotransferase==
-	<StructureSection load='3zzj' size='340' side='right' caption='[[3zzj]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3zzj' size='340' side='right'caption='[[3zzj]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zzj]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ZZJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zzj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZZJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1arg\|1arg]], [[1aam\|1aam]], [[1toe\|1toe]], [[1ix7\|1ix7]], [[1cq6\|1cq6]], [[1asn\|1asn]], [[1g7x\|1g7x]], [[1ahx\|1ahx]], [[1asg\|1asg]], [[1ahy\|1ahy]], [[1qis\|1qis]], [[1g7w\|1g7w]], [[1qir\|1qir]], [[1ahe\|1ahe]], [[1ase\|1ase]], [[1ams\|1ams]], [[2aat\|2aat]], [[1arh\|1arh]], [[1ari\|1ari]], [[1ix8\|1ix8]], [[1bqd\|1bqd]], [[1cq8\|1cq8]], [[1asb\|1asb]], [[1asd\|1asd]], [[1asa\|1asa]], [[1ahf\|1ahf]], [[1art\|1art]], [[1toi\|1toi]], [[5eaa\|5eaa]], [[1czc\|1czc]], [[1aib\|1aib]], [[1ix6\|1ix6]], [[1asm\|1asm]], [[1yoo\|1yoo]], [[1asf\|1asf]], [[1g4x\|1g4x]], [[1c9c\|1c9c]], [[1b4x\|1b4x]], [[1bqa\|1bqa]], [[1spa\|1spa]], [[1tok\|1tok]], [[1toj\|1toj]], [[1aic\|1aic]], [[1cq7\|1cq7]], [[1aaw\|1aaw]], [[1ars\|1ars]], [[1g4v\|1g4v]], [[1aia\|1aia]], [[1asl\|1asl]], [[3aat\|3aat]], [[1asc\|1asc]], [[1tog\|1tog]], [[1amr\|1amr]], [[1cze\|1cze]], [[1qit\|1qit]], [[1ahg\|1ahg]], [[1amq\|1amq]], [[3zzk\|3zzk]], [[4a00\|4a00]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1arg\|1arg]], [[1aam\|1aam]], [[1toe\|1toe]], [[1ix7\|1ix7]], [[1cq6\|1cq6]], [[1asn\|1asn]], [[1g7x\|1g7x]], [[1ahx\|1ahx]], [[1asg\|1asg]], [[1ahy\|1ahy]], [[1qis\|1qis]], [[1g7w\|1g7w]], [[1qir\|1qir]], [[1ahe\|1ahe]], [[1ase\|1ase]], [[1ams\|1ams]], [[2aat\|2aat]], [[1arh\|1arh]], [[1ari\|1ari]], [[1ix8\|1ix8]], [[1bqd\|1bqd]], [[1cq8\|1cq8]], [[1asb\|1asb]], [[1asd\|1asd]], [[1asa\|1asa]], [[1ahf\|1ahf]], [[1art\|1art]], [[1toi\|1toi]], [[5eaa\|5eaa]], [[1czc\|1czc]], [[1aib\|1aib]], [[1ix6\|1ix6]], [[1asm\|1asm]], [[1yoo\|1yoo]], [[1asf\|1asf]], [[1g4x\|1g4x]], [[1c9c\|1c9c]], [[1b4x\|1b4x]], [[1bqa\|1bqa]], [[1spa\|1spa]], [[1tok\|1tok]], [[1toj\|1toj]], [[1aic\|1aic]], [[1cq7\|1cq7]], [[1aaw\|1aaw]], [[1ars\|1ars]], [[1g4v\|1g4v]], [[1aia\|1aia]], [[1asl\|1asl]], [[3aat\|3aat]], [[1asc\|1asc]], [[1tog\|1tog]], [[1amr\|1amr]], [[1cze\|1cze]], [[1qit\|1qit]], [[1ahg\|1ahg]], [[1amq\|1amq]], [[3zzk\|3zzk]], [[4a00\|4a00]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3zzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzj OCA], [~~http~~://pdbe.org/3zzj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3zzj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3zzj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzj OCA], [https://pdbe.org/3zzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zzj RCSB], [https://www.ebi.ac.uk/pdbsum/3zzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
-	*[[Aspartate ~~Aminotransferase~~\|Aspartate ~~Aminotransferase~~]]	+	*[[Aspartate aminotransferase 3D structures\|Aspartate aminotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Aspartate transaminase]]		[[Category: Aspartate transaminase]]
		+	[[Category: Large Structures]]
	[[Category: Christen, P]]		[[Category: Christen, P]]
	[[Category: Coll, M]]		[[Category: Coll, M]]

OCA at 16:43, 27 February 2019

2019-02-27T16:43:32Z

←Older revision		Revision as of 16:43, 27 February 2019
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	[[Category: Christen, P]]		[[Category: Christen, P]]
	[[Category: Coll, M]]		[[Category: Coll, M]]
-	[[Category: Devries, D]]
	[[Category: Fernandez, F J]]		[[Category: Fernandez, F J]]
	[[Category: Gehring, H]]		[[Category: Gehring, H]]
	[[Category: Pena-Soler, E]]		[[Category: Pena-Soler, E]]
	[[Category: Vega, M C]]		[[Category: Vega, M C]]
		+	[[Category: DeVries, D]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 18:54, 10 December 2016

2016-12-10T18:54:07Z

←Older revision		Revision as of 18:54, 10 December 2016
Line 1:		Line 1:
		+
	==Structure of an engineered aspartate aminotransferase==		==Structure of an engineered aspartate aminotransferase==
	<StructureSection load='3zzj' size='340' side='right' caption='[[3zzj]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3zzj' size='340' side='right' caption='[[3zzj]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3zzj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3zzj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1arg\|1arg]], [[1aam\|1aam]], [[1toe\|1toe]], [[1ix7\|1ix7]], [[1cq6\|1cq6]], [[1asn\|1asn]], [[1g7x\|1g7x]], [[1ahx\|1ahx]], [[1asg\|1asg]], [[1ahy\|1ahy]], [[1qis\|1qis]], [[1g7w\|1g7w]], [[1qir\|1qir]], [[1ahe\|1ahe]], [[1ase\|1ase]], [[1ams\|1ams]], [[2aat\|2aat]], [[1arh\|1arh]], [[1ari\|1ari]], [[1ix8\|1ix8]], [[1bqd\|1bqd]], [[1cq8\|1cq8]], [[1asb\|1asb]], [[1asd\|1asd]], [[1asa\|1asa]], [[1ahf\|1ahf]], [[1art\|1art]], [[1toi\|1toi]], [[5eaa\|5eaa]], [[1czc\|1czc]], [[1aib\|1aib]], [[1ix6\|1ix6]], [[1asm\|1asm]], [[1yoo\|1yoo]], [[1asf\|1asf]], [[1g4x\|1g4x]], [[1c9c\|1c9c]], [[1b4x\|1b4x]], [[1bqa\|1bqa]], [[1spa\|1spa]], [[1tok\|1tok]], [[1toj\|1toj]], [[1aic\|1aic]], [[1cq7\|1cq7]], [[1aaw\|1aaw]], [[1ars\|1ars]], [[1g4v\|1g4v]], [[1aia\|1aia]], [[1asl\|1asl]], [[3aat\|3aat]], [[1asc\|1asc]], [[1tog\|1tog]], [[1amr\|1amr]], [[1cze\|1cze]], [[1qit\|1qit]], [[1ahg\|1ahg]], [[1amq\|1amq]], [[3zzk\|3zzk]], [[4a00\|4a00]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1arg\|1arg]], [[1aam\|1aam]], [[1toe\|1toe]], [[1ix7\|1ix7]], [[1cq6\|1cq6]], [[1asn\|1asn]], [[1g7x\|1g7x]], [[1ahx\|1ahx]], [[1asg\|1asg]], [[1ahy\|1ahy]], [[1qis\|1qis]], [[1g7w\|1g7w]], [[1qir\|1qir]], [[1ahe\|1ahe]], [[1ase\|1ase]], [[1ams\|1ams]], [[2aat\|2aat]], [[1arh\|1arh]], [[1ari\|1ari]], [[1ix8\|1ix8]], [[1bqd\|1bqd]], [[1cq8\|1cq8]], [[1asb\|1asb]], [[1asd\|1asd]], [[1asa\|1asa]], [[1ahf\|1ahf]], [[1art\|1art]], [[1toi\|1toi]], [[5eaa\|5eaa]], [[1czc\|1czc]], [[1aib\|1aib]], [[1ix6\|1ix6]], [[1asm\|1asm]], [[1yoo\|1yoo]], [[1asf\|1asf]], [[1g4x\|1g4x]], [[1c9c\|1c9c]], [[1b4x\|1b4x]], [[1bqa\|1bqa]], [[1spa\|1spa]], [[1tok\|1tok]], [[1toj\|1toj]], [[1aic\|1aic]], [[1cq7\|1cq7]], [[1aaw\|1aaw]], [[1ars\|1ars]], [[1g4v\|1g4v]], [[1aia\|1aia]], [[1asl\|1asl]], [[3aat\|3aat]], [[1asc\|1asc]], [[1tog\|1tog]], [[1amr\|1amr]], [[1cze\|1cze]], [[1qit\|1qit]], [[1ahg\|1ahg]], [[1amq\|1amq]], [[3zzk\|3zzk]], [[4a00\|4a00]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zzj RCSB], [http://www.ebi.ac.uk/pdbsum/3zzj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzj OCA], [http://pdbe.org/3zzj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zzj RCSB], [http://www.ebi.ac.uk/pdbsum/3zzj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3zzj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Aspartate transaminase]]		[[Category: Aspartate transaminase]]
-	[[Category: Escherichia coli]]
	[[Category: Christen, P]]		[[Category: Christen, P]]
	[[Category: Coll, M]]		[[Category: Coll, M]]

OCA at 17:09, 9 December 2014

2014-12-09T17:09:14Z

←Older revision		Revision as of 17:09, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3zzj.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of an engineered aspartate aminotransferase==
		+	<StructureSection load='3zzj' size='340' side='right' caption='[[3zzj]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3zzj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZZJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1arg\|1arg]], [[1aam\|1aam]], [[1toe\|1toe]], [[1ix7\|1ix7]], [[1cq6\|1cq6]], [[1asn\|1asn]], [[1g7x\|1g7x]], [[1ahx\|1ahx]], [[1asg\|1asg]], [[1ahy\|1ahy]], [[1qis\|1qis]], [[1g7w\|1g7w]], [[1qir\|1qir]], [[1ahe\|1ahe]], [[1ase\|1ase]], [[1ams\|1ams]], [[2aat\|2aat]], [[1arh\|1arh]], [[1ari\|1ari]], [[1ix8\|1ix8]], [[1bqd\|1bqd]], [[1cq8\|1cq8]], [[1asb\|1asb]], [[1asd\|1asd]], [[1asa\|1asa]], [[1ahf\|1ahf]], [[1art\|1art]], [[1toi\|1toi]], [[5eaa\|5eaa]], [[1czc\|1czc]], [[1aib\|1aib]], [[1ix6\|1ix6]], [[1asm\|1asm]], [[1yoo\|1yoo]], [[1asf\|1asf]], [[1g4x\|1g4x]], [[1c9c\|1c9c]], [[1b4x\|1b4x]], [[1bqa\|1bqa]], [[1spa\|1spa]], [[1tok\|1tok]], [[1toj\|1toj]], [[1aic\|1aic]], [[1cq7\|1cq7]], [[1aaw\|1aaw]], [[1ars\|1ars]], [[1g4v\|1g4v]], [[1aia\|1aia]], [[1asl\|1asl]], [[3aat\|3aat]], [[1asc\|1asc]], [[1tog\|1tog]], [[1amr\|1amr]], [[1cze\|1cze]], [[1qit\|1qit]], [[1ahg\|1ahg]], [[1amq\|1amq]], [[3zzk\|3zzk]], [[4a00\|4a00]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zzj RCSB], [http://www.ebi.ac.uk/pdbsum/3zzj PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The joint substitution of three active-site residues in Escherichia colil-aspartate aminotransferase increases the ratio of l-cysteine sulfinate desulfinase to transaminase activity 10(5)-fold. This change in reaction specificity results from combining a tyrosine-shift double mutation (Y214Q/R280Y) with a non-conservative substitution of a substrate-binding residue (I33Q). Tyr214 hydrogen bonds with O3 of the cofactor and is close to Arg374 which binds the alpha-carboxylate group of the substrate; Arg280 interacts with the distal carboxylate group of the substrate; and Ile33 is part of the hydrophobic patch near the entrance to the active site, presumably participating in the domain closure essential for the transamination reaction. In the triple-mutant enzyme, k(cat)' for desulfination of l-cysteine sulfinate increased to 0.5s(-1) (from 0.05s(-1) in wild-type enzyme), whereas k(cat)' for transamination of the same substrate was reduced from 510s(-1) to 0.05s(-1). Similarly, k(cat)' for beta-decarboxylation of l-aspartate increased from<0.0001s(-1) to 0.07s(-1), whereas k(cat)' for transamination was reduced from 530s(-1) to 0.13s(-1). l-Aspartate aminotransferase had thus been converted into an l-cysteine sulfinate desulfinase that catalyzes transamination and l-aspartate beta-decarboxylation as side reactions. The X-ray structures of the engineered l-cysteine sulfinate desulfinase in its pyridoxal-5'-phosphate and pyridoxamine-5'-phosphate form or liganded with a covalent coenzyme-substrate adduct identified the subtle structural changes that suffice for generating desulfinase activity and concomitantly abolishing transaminase activity toward dicarboxylic amino acids. Apparently, the triple mutation impairs the domain closure thus favoring reprotonation of alternative acceptor sites in coenzyme-substrate intermediates by bulk water.

-	~~{{STRUCTURE_3zzj\| PDB=3zzj \| SCENE= }}~~	+	Structure and mechanism of a cysteine sulfinate desulfinase engineered on the aspartate aminotransferase scaffold.,Fernandez FJ, de Vries D, Pena-Soler E, Coll M, Christen P, Gehring H, Vega MC Biochim Biophys Acta. 2011 Nov 23;1824(2):339-349. PMID:22138634<ref>PMID:22138634</ref>

-	~~===Structure~~ of ~~an engineered aspartate aminotransferase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22138634}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3zzj]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA].~~	+

	==See Also==		==See Also==
	*[[Aspartate Aminotransferase\|Aspartate Aminotransferase]]		*[[Aspartate Aminotransferase\|Aspartate Aminotransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022138634<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Aspartate transaminase]]		[[Category: Aspartate transaminase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Christen, P.]]	+	[[Category: Christen, P]]
-	[[Category: Coll, M.]]	+	[[Category: Coll, M]]
-	[[Category: Devries, D.]]	+	[[Category: Devries, D]]
-	[[Category: Fernandez, F J.]]	+	[[Category: Fernandez, F J]]
-	[[Category: Gehring, H.]]	+	[[Category: Gehring, H]]
-	[[Category: Pena-Soler, E.]]	+	[[Category: Pena-Soler, E]]
-	[[Category: Vega, M C.]]	+	[[Category: Vega, M C]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 09:59, 20 October 2012

2012-10-20T09:59:55Z

←Older revision		Revision as of 09:59, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3zzj]] is a 1 chain structure ~~of [[Aspartate Aminotransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA].	+	[[3zzj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA].

	==See Also==		==See Also==

OCA at 00:39, 26 July 2012

2012-07-26T00:39:13Z

←Older revision		Revision as of 00:39, 26 July 2012
Line 1:		Line 1:
-	[[Image:3zzj.~~jpg~~\|left\|200px]]	+	[[Image:3zzj.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3zzj", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3zzj\| PDB=3zzj \| SCENE= }}		{{STRUCTURE_3zzj\| PDB=3zzj \| SCENE= }}

	===Structure of an engineered aspartate aminotransferase===		===Structure of an engineered aspartate aminotransferase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22138634}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22138634 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22138634}}		{{ABSTRACT_PUBMED_22138634}}

	==About this Structure==		==About this Structure==
-	[[3zzj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA].	+	[[3zzj]] is a 1 chain structure of [[Aspartate Aminotransferase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA].
		+
		+	==See Also==
		+	*[[Aspartate Aminotransferase\|Aspartate Aminotransferase]]

	==Reference==		==Reference==

OCA at 07:14, 28 December 2011

2011-12-28T07:14:52Z

←Older revision		Revision as of 07:14, 28 December 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3zzj.jpg\|left\|200px]]

-	The ~~entry 3zzj~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3zzj", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3zzj\| PDB=3zzj \| SCENE= }}

-	~~Authors: Fernandez, F.J., deVries, D., Pena-Soler, E., Coll, M., Christen, P., Gehring, H., Vega, M.C.~~	+	===Structure of an engineered aspartate aminotransferase===

-	~~Description~~: Structure ~~of an engineered aspartate aminotransferase~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22138634}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22138634 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22138634}}
		+
		+	==About this Structure==
		+	[[3zzj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZJ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022138634</ref><references group="xtra"/>
		+	[[Category: Aspartate transaminase]]
		+	[[Category: Escherichia coli]]
		+	[[Category: Christen, P.]]
		+	[[Category: Coll, M.]]
		+	[[Category: Devries, D.]]
		+	[[Category: Fernandez, F J.]]
		+	[[Category: Gehring, H.]]
		+	[[Category: Pena-Soler, E.]]
		+	[[Category: Vega, M C.]]
		+	[[Category: Transferase]]

OCA at 06:09, 14 September 2011

2011-09-14T06:09:42Z

←Older revision		Revision as of 06:09, 14 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3zzj is ON HOLD ~~until sometime in the future~~	+	The entry 3zzj is ON HOLD

	Authors: Fernandez, F.J., deVries, D., Pena-Soler, E., Coll, M., Christen, P., Gehring, H., Vega, M.C.		Authors: Fernandez, F.J., deVries, D., Pena-Soler, E., Coll, M., Christen, P., Gehring, H., Vega, M.C.

	Description: Structure of an engineered aspartate aminotransferase		Description: Structure of an engineered aspartate aminotransferase

OCA: Protected "3zzj" [edit=sysop:move=sysop]

2011-09-07T05:54:59Z

Protected "3zzj" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:54, 7 September 2011