4a02 - Revision history

OCA at 11:17, 20 December 2023

2023-12-20T11:17:09Z

←Older revision		Revision as of 11:17, 20 December 2023
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	<StructureSection load='4a02' size='340' side='right'caption='[[4a02]], [[Resolution\|resolution]] 0.95Å' scene=''>		<StructureSection load='4a02' size='340' side='right'caption='[[4a02]], [[Resolution\|resolution]] 0.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4a02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A02 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4a02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A02 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a02 OCA], [https://pdbe.org/4a02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a02 RCSB], [https://www.ebi.ac.uk/pdbsum/4a02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a02 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 0.95Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a02 OCA], [https://pdbe.org/4a02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a02 RCSB], [https://www.ebi.ac.uk/pdbsum/4a02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a02 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LCHMO_ENTFA LCHMO_ENTFA] Involved in chitin degradation. Catalyzes the oxidative cleavage of glycosidic bonds in both alpha- and beta-chitin via a copper-dependent mechanism, leading to oxidized chitooligosaccharides with a dominance of even-numbered products. Acts synergistically with the chitinase EfChi18A, and combining the two enzymes leads to rapid and complete depolymerization of crystalline chitin, especially with beta-chitin as a substrate. Is likely involved in a chitin degradation pathway that allows E.faecalis V583 to grow on chitin as a carbon source.<ref>PMID:22210154</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterococcus ~~proteiformis thiercelin and jouhaud 1903~~]]	+	[[Category: Enterococcus faecalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bjoras, M]]	+	[[Category: Bjoras M]]
-	[[Category: Bohle~~, L A~~]]	+	[[Category: Bohle LA]]
-	[[Category: Dalhus, B]]	+	[[Category: Dalhus B]]
-	[[Category: Eijsink~~, V G.H~~]]	+	[[Category: Eijsink VGH]]
-	[[Category: Gaseidnes, S]]	+	[[Category: Gaseidnes S]]
-	[[Category: Mathiesen, G]]	+	[[Category: Mathiesen G]]
-	[[Category: Vaaje-Kolstad, G]]	+	[[Category: Vaaje-Kolstad G]]
-	~~[[Category: Chitin binding protein]]~~	+
-	~~[[Category: Chitin degradation]]~~	+
-	~~[[Category: Chitin oxidation~~]]	+

OCA at 07:35, 18 August 2022

2022-08-18T07:35:54Z

←Older revision		Revision as of 07:35, 18 August 2022
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	==X-ray crystallographic structure of EfCBM33A==		==X-ray crystallographic structure of EfCBM33A==
-	<StructureSection load='4a02' size='340' side='right' caption='[[4a02]], [[Resolution\|resolution]] 0.95Å' scene=''>	+	<StructureSection load='4a02' size='340' side='right'caption='[[4a02]], [[Resolution\|resolution]] 0.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4a02]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A02 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4A02 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4a02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A02 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a02 OCA], [~~http~~://pdbe.org/4a02 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4a02 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4a02 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4a02 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a02 OCA], [https://pdbe.org/4a02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a02 RCSB], [https://www.ebi.ac.uk/pdbsum/4a02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a02 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]		[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
		+	[[Category: Large Structures]]
	[[Category: Bjoras, M]]		[[Category: Bjoras, M]]
	[[Category: Bohle, L A]]		[[Category: Bohle, L A]]

OCA at 19:03, 10 December 2016

2016-12-10T19:03:07Z

←Older revision		Revision as of 19:03, 10 December 2016
Line 1:		Line 1:
		+
	==X-ray crystallographic structure of EfCBM33A==		==X-ray crystallographic structure of EfCBM33A==
	<StructureSection load='4a02' size='340' side='right' caption='[[4a02]], [[Resolution\|resolution]] 0.95Å' scene=''>		<StructureSection load='4a02' size='340' side='right' caption='[[4a02]], [[Resolution\|resolution]] 0.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4a02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterococcus_faecalis Enterococcus faecalis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A02 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4a02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A02 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a02 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a02 RCSB], [http://www.ebi.ac.uk/pdbsum/4a02 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a02 OCA], [http://pdbe.org/4a02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a02 RCSB], [http://www.ebi.ac.uk/pdbsum/4a02 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a02 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4a02" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterococcus ~~faecalis~~]]	+	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
	[[Category: Bjoras, M]]		[[Category: Bjoras, M]]
	[[Category: Bohle, L A]]		[[Category: Bohle, L A]]

OCA at 15:53, 9 December 2014

2014-12-09T15:53:50Z

←Older revision		Revision as of 15:53, 9 December 2014
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-	[[~~Image~~:4a02.~~png\|left\|200px~~]]	+	==X-ray crystallographic structure of EfCBM33A==
		+	<StructureSection load='4a02' size='340' side='right' caption='[[4a02]], [[Resolution\|resolution]] 0.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4a02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A02 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a02 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a02 RCSB], [http://www.ebi.ac.uk/pdbsum/4a02 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Little information exists for the ability of enterococci to utilize chitin as a carbon source. We show that Enterococcus faecalis V583 can grow on chitin, and we describe two proteins, a family 18 chitinase (ef0361; EfChi18A) and a family 33 CBM (carbohydrate binding module) (ef0362; EfCBM33A) that catalyze chitin conversion in vitro. Various types of enzyme activity assays showed that EfChi18A has functional properties characteristic of an endochitinase. EfCBM33A belongs to a recently discovered family of enzymes that cleave glycosidic bonds via an oxidative mechanism and that act synergistically with classical hydrolytic enzymes (i.e., chitinases). The structure and function of this protein were probed in detail. An ultra-high-resolution crystal structure of EfCBM33A revealed details of a conserved binding surface that is optimized to interact with chitin and contains the catalytic center. Chromatography and mass spectrometry analyses of product formation showed that EfCBM33A cleaves chitin via the oxidative mechanism previously described for CBP21 from Serratia marcescens. Metal-depletion studies showed that EfCBM33A is a copper enzyme. In the presence of an external electron donor, EfCBM33A boosted the activity of EfChi18A, and combining the two enzymes led to rapid and complete conversion of beta-chitin to chitobiose. This study provides insight into the structure and function of the CBM33 family of enzymes, which, together with their fungal counterpart called GH61, currently receive considerable attention in the biomass processing field.

-	~~{{STRUCTURE_4a02\| PDB=4a02 \| SCENE= }}~~	+	Characterization of the Chitinolytic Machinery of Enterococcus faecalis V583 and High-Resolution Structure of Its Oxidative CBM33 Enzyme.,Vaaje-Kolstad G, Bohle LA, Gaseidnes S, Dalhus B, Bjoras M, Mathiesen G, Eijsink VG J Mol Biol. 2011 Dec 22. PMID:22210154<ref>PMID:22210154</ref>

-	~~===X-ray crystallographic structure~~ of ~~EfCBM33A===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22210154}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[4a02]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterococcus_faecalis Enterococcus faecalis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4A02 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022210154<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Enterococcus faecalis]]		[[Category: Enterococcus faecalis]]
-	[[Category: Bjoras, M.]]	+	[[Category: Bjoras, M]]
-	[[Category: Bohle, L A.]]	+	[[Category: Bohle, L A]]
-	[[Category: Dalhus, B.]]	+	[[Category: Dalhus, B]]
-	[[Category: Eijsink, V G.H.]]	+	[[Category: Eijsink, V G.H]]
-	[[Category: Gaseidnes, S.]]	+	[[Category: Gaseidnes, S]]
-	[[Category: Mathiesen, G.]]	+	[[Category: Mathiesen, G]]
-	[[Category: Vaaje-Kolstad, G.]]	+	[[Category: Vaaje-Kolstad, G]]
	[[Category: Chitin binding protein]]		[[Category: Chitin binding protein]]
	[[Category: Chitin degradation]]		[[Category: Chitin degradation]]
	[[Category: Chitin oxidation]]		[[Category: Chitin oxidation]]

OCA at 09:52, 21 November 2012

2012-11-21T09:52:41Z

←Older revision		Revision as of 09:52, 21 November 2012
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	[[Image:4a02.png\|left\|200px]]		[[Image:4a02.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_4a02", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_4a02\| PDB=4a02 \| SCENE= }}		{{STRUCTURE_4a02\| PDB=4a02 \| SCENE= }}

	===X-ray crystallographic structure of EfCBM33A===		===X-ray crystallographic structure of EfCBM33A===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22210154}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22210154 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22210154}}		{{ABSTRACT_PUBMED_22210154}}

OCA at 06:21, 11 January 2012

2012-01-11T06:21:31Z

←Older revision		Revision as of 06:21, 11 January 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4a02.png\|left\|200px]]

-	The ~~entry 4a02~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4a02", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4a02\| PDB=4a02 \| SCENE= }}

-	~~Authors: Vaaje~~-~~Kolstad, G., Bohle, L.A., Gaseidnes, S., Dalhus, B., Bjoras, M., Mathiesen, G., Eijsink, V.G.H.~~	+	===X-ray crystallographic structure of EfCBM33A===

-	~~Description~~: X-~~ray crystallographic~~ structure ~~of EfCBM33A~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22210154}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22210154 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22210154}}
		+
		+	==About this Structure==
		+	[[4a02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A02 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022210154</ref><references group="xtra"/>
		+	[[Category: Enterococcus faecalis]]
		+	[[Category: Bjoras, M.]]
		+	[[Category: Bohle, L A.]]
		+	[[Category: Dalhus, B.]]
		+	[[Category: Eijsink, V G.H.]]
		+	[[Category: Gaseidnes, S.]]
		+	[[Category: Mathiesen, G.]]
		+	[[Category: Vaaje-Kolstad, G.]]
		+	[[Category: Chitin binding protein]]
		+	[[Category: Chitin degradation]]
		+	[[Category: Chitin oxidation]]

OCA at 09:00, 30 October 2011

2011-10-30T09:00:00Z

←Older revision		Revision as of 09:00, 30 October 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4a02 is ON HOLD	+	The entry 4a02 is ON HOLD until Paper Publication

	Authors: Vaaje-Kolstad, G., Bohle, L.A., Gaseidnes, S., Dalhus, B., Bjoras, M., Mathiesen, G., Eijsink, V.G.H.		Authors: Vaaje-Kolstad, G., Bohle, L.A., Gaseidnes, S., Dalhus, B., Bjoras, M., Mathiesen, G., Eijsink, V.G.H.

	Description: X-ray crystallographic structure of EfCBM33A		Description: X-ray crystallographic structure of EfCBM33A

OCA: Protected "4a02" [edit=sysop:move=sysop]

2011-09-14T06:10:13Z

Protected "4a02" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:10, 14 September 2011

OCA: New page: '''Unreleased structure''' The entry 4a02 is ON HOLD Authors: Vaaje-Kolstad, G., Bohle, L.A., Gaseidnes, S., Dalhus, B., Bjoras, M., Mathiesen, G., Eijsink, V.G.H. Description: X-ray c...

2011-09-14T06:10:12Z

New page: '''Unreleased structure''' The entry 4a02 is ON HOLD Authors: Vaaje-Kolstad, G., Bohle, L.A., Gaseidnes, S., Dalhus, B., Bjoras, M., Mathiesen, G., Eijsink, V.G.H. Description: X-ray c...

New page

'''Unreleased structure'''

The entry 4a02 is ON HOLD

Authors: Vaaje-Kolstad, G., Bohle, L.A., Gaseidnes, S., Dalhus, B., Bjoras, M., Mathiesen, G., Eijsink, V.G.H.

Description: X-ray crystallographic structure of EfCBM33A