4ala - Revision history

OCA at 05:37, 25 August 2022

2022-08-25T05:37:54Z

←Older revision		Revision as of 05:37, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='4ala' size='340' side='right'caption='[[4ala]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='4ala' size='340' side='right'caption='[[4ala]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ala]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [~~http~~://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ALA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ala]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ALA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4al8\|4al8]], [[4am0\|4am0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4al8\|4al8]], [[4am0\|4am0]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ala FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ala OCA], [~~http~~://pdbe.org/4ala PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ala RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ala PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ala ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ala FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ala OCA], [https://pdbe.org/4ala PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ala RCSB], [https://www.ebi.ac.uk/pdbsum/4ala PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ala ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/Q7TGD1_9FLAV Q7TGD1_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774]	+	[[https://www.uniprot.org/uniprot/Q7TGD1_9FLAV Q7TGD1_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Dengue virus 3]]		[[Category: Dengue virus 3]]
		+	[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Flanagan, A]]		[[Category: Flanagan, A]]

OCA at 09:37, 6 March 2019

2019-03-06T09:37:20Z

←Older revision		Revision as of 09:37, 6 March 2019
Line 1:		Line 1:

	==Structure of Dengue virus DIII in complex with Fab 2H12==		==Structure of Dengue virus DIII in complex with Fab 2H12==
-	<StructureSection load='4ala' size='340' side='right' caption='[[4ala]], [[Resolution\|resolution]] 1.84Å' scene=''>	+	<StructureSection load='4ala' size='340' side='right'caption='[[4ala]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ala]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ALA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ala]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ALA FirstGlance]. <br>

OCA at 16:42, 5 August 2016

2016-08-05T16:42:23Z

←Older revision		Revision as of 16:42, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of Dengue virus DIII in complex with Fab 2H12==		==Structure of Dengue virus DIII in complex with Fab 2H12==
	<StructureSection load='4ala' size='340' side='right' caption='[[4ala]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='4ala' size='340' side='right' caption='[[4ala]], [[Resolution\|resolution]] 1.84Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4al8\|4al8]], [[4am0\|4am0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4al8\|4al8]], [[4am0\|4am0]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ala FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ala OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ala RCSB], [http://www.ebi.ac.uk/pdbsum/4ala PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ala FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ala OCA], [http://pdbe.org/4ala PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ala RCSB], [http://www.ebi.ac.uk/pdbsum/4ala PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ala ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ala" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:05, 25 December 2014

2014-12-25T04:05:55Z

←Older revision		Revision as of 04:05, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ala]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ALA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ala]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ALA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4al8\|4al8]], [[4am0\|4am0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4al8\|4al8]], [[4am0\|4am0]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ala FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ala OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ala RCSB], [http://www.ebi.ac.uk/pdbsum/4ala PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ala FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ala OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ala RCSB], [http://www.ebi.ac.uk/pdbsum/4ala PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q7TGD1_9FLAV Q7TGD1_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	[[Category: Dengue virus 3]]		[[Category: Dengue virus 3]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Flanagan, A.]]	+	[[Category: Flanagan, A]]
-	[[Category: Grimes, J M.]]	+	[[Category: Grimes, J M]]
-	[[Category: Midgley, C M.]]	+	[[Category: Midgley, C M]]
-	[[Category: Mongkolsapaya, J.]]	+	[[Category: Mongkolsapaya, J]]
-	[[Category: Screaton, G R.]]	+	[[Category: Screaton, G R]]
	[[Category: Antibody]]		[[Category: Antibody]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Neutralisation]]		[[Category: Neutralisation]]

OCA at 08:28, 5 June 2014

2014-06-05T08:28:12Z

←Older revision		Revision as of 08:28, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4ala.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Structure of Dengue virus DIII in complex with Fab 2H12==
		+	<StructureSection load='4ala' size='340' side='right' caption='[[4ala]], [[Resolution\|resolution]] 1.84Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ala]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ALA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4al8\|4al8]], [[4am0\|4am0]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ala FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ala OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ala RCSB], [http://www.ebi.ac.uk/pdbsum/4ala PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dengue virus infections are still increasing at an alarming rate in tropical and subtropical countries, underlying the need for a dengue vaccine. Although it is relatively easy to generate Ab responses to dengue virus, low avidity or low concentrations of Ab may enhance infection of FcR-bearing cells with clinical impact, posing a challenge to vaccine production. In this article, we report the characterization of a mAb, 2H12, which is cross-reactive to all four serotypes in the dengue virus group. Crystal structures of 2H12-Fab in complex with domain III of the envelope protein from three dengue serotypes have been determined. 2H12 binds to the highly conserved AB loop of domain III of the envelope protein that is poorly accessible in the mature virion. 2H12 neutralization varied between dengue serotypes and strains; in particular, dengue serotype 2 was not neutralized. Because the 2H12-binding epitope was conserved, this variation in neutralization highlights differences between dengue serotypes and suggests that significant conformational changes in the virus must take place for Ab binding. Surprisingly, 2H12 facilitated little or no enhancement of infection. These data provide a structural basis for understanding Ab neutralization and enhancement of infection, which is crucial for the development of future dengue vaccines.

-	<!--	+	Structural Analysis of a Dengue Cross-Reactive Antibody Complexed with Envelope Domain III Reveals the Molecular Basis of Cross-Reactivity.,Midgley CM, Flanagan A, Tran HB, Dejnirattisai W, Chawansuntati K, Jumnainsong A, Wongwiwat W, Duangchinda T, Mongkolsapaya J, Grimes JM, Screaton GR J Immunol. 2012 May 15;188(10):4971-9. Epub 2012 Apr 9. PMID:22491255<ref>PMID:22491255</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_4ala"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_4ala\| PDB=4ala \| SCENE= }}~~	+

-	~~===Structure~~ of ~~Dengue virus DIII in complex with Fab 2H12===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22491255}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22491255 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22491255}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[4ala]] is a 3 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALA OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022491255<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Dengue virus 3]]		[[Category: Dengue virus 3]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 07:41, 6 June 2012

2012-06-06T07:41:36Z

←Older revision		Revision as of 07:41, 6 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4ala.jpg\|left\|200px]]

-	The ~~entry 4ala~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4ala", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4ala\| PDB=4ala \| SCENE= }}

-	~~Authors: Midgley, C.M., Flanagan, A., Mongkolsapaya, J., Grimes, J.M., Screaton, G.R.~~	+	===Structure of Dengue virus DIII in complex with Fab 2H12===

-	~~Description~~: Structure of Dengue virus ~~DIII in complex with Fab 2H12~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22491255}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22491255 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22491255}}
		+
		+	==About this Structure==
		+	[[4ala]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ALA OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022491255</ref><references group="xtra"/>
		+	[[Category: Dengue virus 3]]
		+	[[Category: Mus musculus]]
		+	[[Category: Flanagan, A.]]
		+	[[Category: Grimes, J M.]]
		+	[[Category: Midgley, C M.]]
		+	[[Category: Mongkolsapaya, J.]]
		+	[[Category: Screaton, G R.]]
		+	[[Category: Antibody]]
		+	[[Category: Immune system]]
		+	[[Category: Neutralisation]]

OCA at 08:45, 25 April 2012

2012-04-25T08:45:03Z

←Older revision		Revision as of 08:45, 25 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ala is ON HOLD	+	The entry 4ala is ON HOLD until Paper Publication

	Authors: Midgley, C.M., Flanagan, A., Mongkolsapaya, J., Grimes, J.M., Screaton, G.R.		Authors: Midgley, C.M., Flanagan, A., Mongkolsapaya, J., Grimes, J.M., Screaton, G.R.

	Description: Structure of Dengue virus DIII in complex with Fab 2H12		Description: Structure of Dengue virus DIII in complex with Fab 2H12

OCA: Protected "4ala" [edit=sysop:move=sysop]

2012-03-14T06:07:44Z

Protected "4ala" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:07, 14 March 2012

OCA: New page: '''Unreleased structure''' The entry 4ala is ON HOLD Authors: Midgley, C.M., Flanagan, A., Mongkolsapaya, J., Grimes, J.M., Screaton, G.R. Description: Structure of Dengue virus DIII i...

2012-03-14T06:07:43Z

New page: '''Unreleased structure''' The entry 4ala is ON HOLD Authors: Midgley, C.M., Flanagan, A., Mongkolsapaya, J., Grimes, J.M., Screaton, G.R. Description: Structure of Dengue virus DIII i...

New page

'''Unreleased structure'''

The entry 4ala is ON HOLD

Authors: Midgley, C.M., Flanagan, A., Mongkolsapaya, J., Grimes, J.M., Screaton, G.R.

Description: Structure of Dengue virus DIII in complex with Fab 2H12