4aqu - Revision history

OCA at 11:33, 20 December 2023

2023-12-20T11:33:22Z

←Older revision		Revision as of 11:33, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4aqu' size='340' side='right'caption='[[4aqu]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4aqu' size='340' side='right'caption='[[4aqu]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4aqu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chlre Chlre~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AQU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4aqu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AQU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max-height: 3em;~~'>[[1af5\|1af5]], [[1bp7\|1bp7]], [[1g9y\|1g9y]], [[1g9z\|1g9z]], [[1mow\|1mow]], [[1n3e\|1n3e]], [[1n3f\|1n3f]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1u0c\|1u0c]], [[1u0d\|1u0d]], [[2vbj\|2vbj]], [[2vbl\|2vbl]], [[2vbn\|2vbn]], [[2vbo\|2vbo]], [[4aab\|4aab]], [[4aad\|4aad]], [[4aae\|4aae]], [[4aaf\|4aaf]], [[4aag\|4aag]]</~~div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqu OCA], [https://pdbe.org/4aqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aqu RCSB], [https://www.ebi.ac.uk/pdbsum/4aqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aqu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqu OCA], [https://pdbe.org/4aqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aqu RCSB], [https://www.ebi.ac.uk/pdbsum/4aqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aqu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.	+	[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chlre~~]]	+	[[Category: Chlamydomonas reinhardtii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Daboussi, F]]	+	[[Category: Synthetic construct]]
-	[[Category: Duchateau, P]]	+	[[Category: Daboussi F]]
-	[[Category: Leduc, S]]	+	[[Category: Duchateau P]]
-	[[Category: Macmaster, R]]	+	[[Category: Leduc S]]
-	[[Category: Molina, R]]	+	[[Category: Macmaster R]]
-	[[Category: Montoya, G]]	+	[[Category: Molina R]]
-	[[Category: Redondo, P]]	+	[[Category: Montoya G]]
-	[[Category: Valton, J]]	+	[[Category: Redondo P]]
-	~~[[Category: Gene targeting]]~~	+	[[Category: Valton J]]
-	~~[[Category: Homing endonuclease]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protein-dna interaction~~]]	+

OCA at 05:43, 25 August 2022

2022-08-25T05:43:14Z

←Older revision		Revision as of 05:43, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='4aqu' size='340' side='right'caption='[[4aqu]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4aqu' size='340' side='right'caption='[[4aqu]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4aqu]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4AQU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4aqu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AQU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af5\|1af5]], [[1bp7\|1bp7]], [[1g9y\|1g9y]], [[1g9z\|1g9z]], [[1mow\|1mow]], [[1n3e\|1n3e]], [[1n3f\|1n3f]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1u0c\|1u0c]], [[1u0d\|1u0d]], [[2vbj\|2vbj]], [[2vbl\|2vbl]], [[2vbn\|2vbn]], [[2vbo\|2vbo]], [[4aab\|4aab]], [[4aad\|4aad]], [[4aae\|4aae]], [[4aaf\|4aaf]], [[4aag\|4aag]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1af5\|1af5]], [[1bp7\|1bp7]], [[1g9y\|1g9y]], [[1g9z\|1g9z]], [[1mow\|1mow]], [[1n3e\|1n3e]], [[1n3f\|1n3f]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1u0c\|1u0c]], [[1u0d\|1u0d]], [[2vbj\|2vbj]], [[2vbl\|2vbl]], [[2vbn\|2vbn]], [[2vbo\|2vbo]], [[4aab\|4aab]], [[4aad\|4aad]], [[4aae\|4aae]], [[4aaf\|4aaf]], [[4aag\|4aag]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqu OCA], [~~http~~://pdbe.org/4aqu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4aqu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4aqu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4aqu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqu OCA], [https://pdbe.org/4aqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aqu RCSB], [https://www.ebi.ac.uk/pdbsum/4aqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aqu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.	+	[[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Endonuclease\|Endonuclease]]	+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Chlre]]		[[Category: Chlre]]
		+	[[Category: Large Structures]]
	[[Category: Daboussi, F]]		[[Category: Daboussi, F]]
	[[Category: Duchateau, P]]		[[Category: Duchateau, P]]

OCA at 09:13, 6 March 2019

2019-03-06T09:13:54Z

←Older revision		Revision as of 09:13, 6 March 2019
Line 1:		Line 1:

	==Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium==		==Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium==
-	<StructureSection load='4aqu' size='340' side='right' caption='[[4aqu]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4aqu' size='340' side='right'caption='[[4aqu]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AQU FirstGlance]. <br>		<table><tr><td colspan='2'>[[4aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AQU FirstGlance]. <br>

OCA at 18:50, 5 August 2016

2016-08-05T18:50:15Z

←Older revision		Revision as of 18:50, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium==		==Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium==
	<StructureSection load='4aqu' size='340' side='right' caption='[[4aqu]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4aqu' size='340' side='right' caption='[[4aqu]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chlamydomonas_reinhardtii Chlamydomonas reinhardtii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AQU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AQU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af5\|1af5]], [[1bp7\|1bp7]], [[1g9y\|1g9y]], [[1g9z\|1g9z]], [[1mow\|1mow]], [[1n3e\|1n3e]], [[1n3f\|1n3f]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1u0c\|1u0c]], [[1u0d\|1u0d]], [[2vbj\|2vbj]], [[2vbl\|2vbl]], [[2vbn\|2vbn]], [[2vbo\|2vbo]], [[4aab\|4aab]], [[4aad\|4aad]], [[4aae\|4aae]], [[4aaf\|4aaf]], [[4aag\|4aag]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af5\|1af5]], [[1bp7\|1bp7]], [[1g9y\|1g9y]], [[1g9z\|1g9z]], [[1mow\|1mow]], [[1n3e\|1n3e]], [[1n3f\|1n3f]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1u0c\|1u0c]], [[1u0d\|1u0d]], [[2vbj\|2vbj]], [[2vbl\|2vbl]], [[2vbn\|2vbn]], [[2vbo\|2vbo]], [[4aab\|4aab]], [[4aad\|4aad]], [[4aae\|4aae]], [[4aaf\|4aaf]], [[4aag\|4aag]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aqu RCSB], [http://www.ebi.ac.uk/pdbsum/4aqu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqu OCA], [http://pdbe.org/4aqu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4aqu RCSB], [http://www.ebi.ac.uk/pdbsum/4aqu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4aqu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4aqu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chlamydomonas reinhardtii~~]]	+	[[Category: Chlre]]
	[[Category: Daboussi, F]]		[[Category: Daboussi, F]]
	[[Category: Duchateau, P]]		[[Category: Duchateau, P]]

OCA at 04:57, 25 December 2014

2014-12-25T04:57:24Z

←Older revision		Revision as of 04:57, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AQU FirstGlance]. <br>		<table><tr><td colspan='2'>[[4aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AQU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af5\|1af5]], [[1bp7\|1bp7]], [[1g9y\|1g9y]], [[1g9z\|1g9z]], [[1mow\|1mow]], [[1n3e\|1n3e]], [[1n3f\|1n3f]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1u0c\|1u0c]], [[1u0d\|1u0d]], [[2vbj\|2vbj]], [[2vbl\|2vbl]], [[2vbn\|2vbn]], [[2vbo\|2vbo]], [[4aab\|4aab]], [[4aad\|4aad]], [[4aae\|4aae]], [[4aaf\|4aaf]], [[4aag\|4aag]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af5\|1af5]], [[1bp7\|1bp7]], [[1g9y\|1g9y]], [[1g9z\|1g9z]], [[1mow\|1mow]], [[1n3e\|1n3e]], [[1n3f\|1n3f]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1u0c\|1u0c]], [[1u0d\|1u0d]], [[2vbj\|2vbj]], [[2vbl\|2vbl]], [[2vbn\|2vbn]], [[2vbo\|2vbo]], [[4aab\|4aab]], [[4aad\|4aad]], [[4aae\|4aae]], [[4aaf\|4aaf]], [[4aag\|4aag]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aqu RCSB], [http://www.ebi.ac.uk/pdbsum/4aqu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aqu RCSB], [http://www.ebi.ac.uk/pdbsum/4aqu PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Chlamydomonas reinhardtii]]		[[Category: Chlamydomonas reinhardtii]]
-	[[Category: Daboussi, F.]]	+	[[Category: Daboussi, F]]
-	[[Category: Duchateau, P.]]	+	[[Category: Duchateau, P]]
-	[[Category: Leduc, S.]]	+	[[Category: Leduc, S]]
-	[[Category: Macmaster, R.]]	+	[[Category: Macmaster, R]]
-	[[Category: Molina, R.]]	+	[[Category: Molina, R]]
-	[[Category: Montoya, G.]]	+	[[Category: Montoya, G]]
-	[[Category: Redondo, P.]]	+	[[Category: Redondo, P]]
-	[[Category: Valton, J.]]	+	[[Category: Valton, J]]
	[[Category: Gene targeting]]		[[Category: Gene targeting]]
	[[Category: Homing endonuclease]]		[[Category: Homing endonuclease]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Protein-dna interaction]]		[[Category: Protein-dna interaction]]

OCA at 10:17, 16 June 2014

2014-06-16T10:17:54Z

←Older revision		Revision as of 10:17, 16 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4aqu.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium==
		+	<StructureSection load='4aqu' size='340' side='right' caption='[[4aqu]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AQU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1af5\|1af5]], [[1bp7\|1bp7]], [[1g9y\|1g9y]], [[1g9z\|1g9z]], [[1mow\|1mow]], [[1n3e\|1n3e]], [[1n3f\|1n3f]], [[1t9i\|1t9i]], [[1t9j\|1t9j]], [[1u0c\|1u0c]], [[1u0d\|1u0d]], [[2vbj\|2vbj]], [[2vbl\|2vbl]], [[2vbn\|2vbn]], [[2vbo\|2vbo]], [[4aab\|4aab]], [[4aad\|4aad]], [[4aae\|4aae]], [[4aaf\|4aaf]], [[4aag\|4aag]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aqu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aqu RCSB], [http://www.ebi.ac.uk/pdbsum/4aqu PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In this study, we asked whether CpG methylation could influence the DNA binding affinity and activity of meganucleases used for genome engineering applications. A combination of biochemical and structural approaches enabled us to demonstrate that CpG methylation decreases I-CreI DNA binding affinity and inhibits its endonuclease activity in vitro. This inhibition depends on the position of the methylated cytosine within the DNA target and was almost total when it is located inside the central tetrabase. Crystal structures of I-CreI bound to methylated cognate target DNA suggested a molecular basis for such inhibition, although the precise mechanism still has to be specified. Finally, we demonstrated that the efficacy of engineered meganucleases can be diminished by CpG methylation of the targeted endogenous site, and we proposed a rational design of the meganuclease DNA binding domain to alleviate such an effect. We conclude that although activity and sequence specificity of engineered meganucleases are crucial parameters, target DNA epigenetic modifications need to be considered for successful gene editions.

-	~~{{STRUCTURE_4aqu\| PDB=4aqu \| SCENE= }}~~	+	5'-Cytosine-phosphoguanine (CpG) methylation impacts the activity of natural and engineered meganucleases.,Valton J, Daboussi F, Leduc S, Molina R, Redondo P, Macmaster R, Montoya G, Duchateau P J Biol Chem. 2012 Aug 31;287(36):30139-50. Epub 2012 Jun 27. PMID:22740697<ref>PMID:22740697</ref>

-	~~===Crystal structure~~ of ~~I-CreI complexed with its target methylated at position plus 2 (in~~ the ~~b strand) in the presence~~ of ~~calcium===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22740697}}~~	+	==See Also==
-		+	*[[Endonuclease\|Endonuclease]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4aqu~~]] ~~is a 4 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA].~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Chlamydomonas reinhardtii]]		[[Category: Chlamydomonas reinhardtii]]
	[[Category: Daboussi, F.]]		[[Category: Daboussi, F.]]

OCA at 05:10, 12 September 2012

2012-09-12T05:10:24Z

←Older revision		Revision as of 05:10, 12 September 2012
Line 1:		Line 1:
-	[[Image:4aqu.~~jpg~~\|left\|200px]]	+	[[Image:4aqu.png\|left\|200px]]

	{{STRUCTURE_4aqu\| PDB=4aqu \| SCENE= }}		{{STRUCTURE_4aqu\| PDB=4aqu \| SCENE= }}
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	===Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium===		===Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium===

		+	{{ABSTRACT_PUBMED_22740697}}

	==About this Structure==		==About this Structure==

OCA at 07:46, 4 July 2012

2012-07-04T07:46:45Z

←Older revision		Revision as of 07:46, 4 July 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4aqu.jpg\|left\|200px]]

-	~~The entry~~ 4aqu ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4aqu\| PDB=4aqu \| SCENE= }}

-	~~Authors: Valton, J., Daboussi, F., Leduc, S., Redondo, P., Macmaster, R., Molina, R., Montoya, G., Duchateau, P.~~	+	===Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium===

-	~~Description: Crystal~~ structure ~~of I-CreI complexed~~ with ~~its target methylated at position plus 2 (in the b strand) in the presence of calcium~~	+
		+	==About this Structure==
		+	[[4aqu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AQU OCA].
		+	[[Category: Chlamydomonas reinhardtii]]
		+	[[Category: Daboussi, F.]]
		+	[[Category: Duchateau, P.]]
		+	[[Category: Leduc, S.]]
		+	[[Category: Macmaster, R.]]
		+	[[Category: Molina, R.]]
		+	[[Category: Montoya, G.]]
		+	[[Category: Redondo, P.]]
		+	[[Category: Valton, J.]]
		+	[[Category: Gene targeting]]
		+	[[Category: Homing endonuclease]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protein-dna interaction]]

OCA at 05:14, 27 June 2012

2012-06-27T05:14:53Z

←Older revision		Revision as of 05:14, 27 June 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4aqu is ON HOLD	+	The entry 4aqu is ON HOLD until Paper Publication

	Authors: Valton, J., Daboussi, F., Leduc, S., Redondo, P., Macmaster, R., Molina, R., Montoya, G., Duchateau, P.		Authors: Valton, J., Daboussi, F., Leduc, S., Redondo, P., Macmaster, R., Molina, R., Montoya, G., Duchateau, P.

	Description: Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium		Description: Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium

OCA: Protected "4aqu" [edit=sysop:move=sysop]

2012-04-25T08:48:09Z

Protected "4aqu" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:48, 25 April 2012