4are - Revision history

OCA at 11:33, 20 December 2023

2023-12-20T11:33:48Z

←Older revision		Revision as of 11:33, 20 December 2023
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	<StructureSection load='4are' size='340' side='right'caption='[[4are]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='4are' size='340' side='right'caption='[[4are]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4are]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ARE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4are]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hathewaya_histolytica Hathewaya histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ARE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.19Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2y3u\|2y3u]], [[2y50\|2y50]], [[2y6i\|2y6i]], [[2y72\|2y72]], [[4aqo\|4aqo]], [[4ar9\|4ar9]], [[4arf\|4arf]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4are FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4are OCA], [https://pdbe.org/4are PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4are RCSB], [https://www.ebi.ac.uk/pdbsum/4are PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4are ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4are FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4are OCA], [https://pdbe.org/4are PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4are RCSB], [https://www.ebi.ac.uk/pdbsum/4are PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4are ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/COLG_HATHI COLG_HATHI] Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen (PubMed:3002446). Active on soluble type I collagen, insoluble collagen, azocoll, soluble PZ-peptide (all collagenase substrates) and gelatin (PubMed:9922257). The full-length protein has collagenase activity, while the in vivo derived C-terminally truncated shorter versions only act on gelatin (PubMed:9922257). In vitro digestion of soluble calf skin collagen fibrils requires both ColG and ColH; ColG forms missing the second collagen-binding domain are also synergistic with ColH, although their overall efficiency is decreased (PubMed:18374061, PubMed:22099748). The activator domain (residues 119-388) and catalytic subdomain (389-670) open and close around substrate using a Gly-rich hinge (387-397), allowing digestion when the protein is closed (PubMed:21947205, PubMed:23703618). Binding of collagen requires Ca(2+) and is inhibited by EGTA; the collagen-binding domain (CBD, S3a plus S3b) specifically recognizes the triple-helical conformation made by 3 collagen protein chains in the triple-helical region (PubMed:11121400). Isolated CBD (S3a plus S3b) binds collagen fibrils and sheets of many tissues (PubMed:11913772).<ref>PMID:11121400</ref> <ref>PMID:11913772</ref> <ref>PMID:18374061</ref> <ref>PMID:18937627</ref> <ref>PMID:21947205</ref> <ref>PMID:22099748</ref> <ref>PMID:23703618</ref> <ref>PMID:24125730</ref> <ref>PMID:28820255</ref> <ref>PMID:3002446</ref> <ref>PMID:9922257</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus histolyticus weinberg and seguin 1916~~]]	+	[[Category: Hathewaya histolytica]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Microbial collagenase]]~~	+	[[Category: Brandstetter H]]
-	[[Category: Brandstetter, H]]	+	[[Category: Eckhard U]]
-	[[Category: Eckhard, U]]	+
-	~~[[Category: Collagen]]~~	+
-	~~[[Category: Collagenolysis]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Metalloprotease]]~~	+
-	~~[[Category: Peptidase~~]]	+

OCA at 05:43, 25 August 2022

2022-08-25T05:43:46Z

←Older revision		Revision as of 05:43, 25 August 2022
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	<StructureSection load='4are' size='340' side='right'caption='[[4are]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='4are' size='340' side='right'caption='[[4are]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4are]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ARE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4are]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ARE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2y3u\|2y3u]], [[2y50\|2y50]], [[2y6i\|2y6i]], [[2y72\|2y72]], [[4aqo\|4aqo]], [[4ar9\|4ar9]], [[4arf\|4arf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2y3u\|2y3u]], [[2y50\|2y50]], [[2y6i\|2y6i]], [[2y72\|2y72]], [[4aqo\|4aqo]], [[4ar9\|4ar9]], [[4arf\|4arf]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4are FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4are OCA], [~~http~~://pdbe.org/4are PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4are RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4are PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4are ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4are FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4are OCA], [https://pdbe.org/4are PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4are RCSB], [https://www.ebi.ac.uk/pdbsum/4are PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4are ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
-	*[[Collagenase ~~(non-MMP)~~\|Collagenase ~~(non-MMP)~~]]	+	*[[Collagenase 3D structures\|Collagenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus histolyticus weinberg and seguin 1916]]		[[Category: Bacillus histolyticus weinberg and seguin 1916]]
		+	[[Category: Large Structures]]
	[[Category: Microbial collagenase]]		[[Category: Microbial collagenase]]
	[[Category: Brandstetter, H]]		[[Category: Brandstetter, H]]

OCA at 08:55, 6 March 2019

2019-03-06T08:55:53Z

←Older revision		Revision as of 08:55, 6 March 2019
Line 1:		Line 1:

	==Crystal structure of the collagenase Unit of collagenase G from Clostridium histolyticum at 2.19 angstrom resolution.==		==Crystal structure of the collagenase Unit of collagenase G from Clostridium histolyticum at 2.19 angstrom resolution.==
-	<StructureSection load='4are' size='340' side='right' caption='[[4are]], [[Resolution\|resolution]] 2.19Å' scene=''>	+	<StructureSection load='4are' size='340' side='right'caption='[[4are]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4are]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4are]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARE FirstGlance]. <br>

OCA at 12:01, 5 August 2016

2016-08-05T12:01:37Z

←Older revision		Revision as of 12:01, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the collagenase Unit of collagenase G from Clostridium histolyticum at 2.19 angstrom resolution.==		==Crystal structure of the collagenase Unit of collagenase G from Clostridium histolyticum at 2.19 angstrom resolution.==
	<StructureSection load='4are' size='340' side='right' caption='[[4are]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='4are' size='340' side='right' caption='[[4are]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4are]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_histolyticum Clostridium histolyticum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4are]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_histolyticus"_weinberg_and_seguin_1916 "bacillus histolyticus" weinberg and seguin 1916]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2y3u\|2y3u]], [[2y50\|2y50]], [[2y6i\|2y6i]], [[2y72\|2y72]], [[4aqo\|4aqo]], [[4ar9\|4ar9]], [[4arf\|4arf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2y3u\|2y3u]], [[2y50\|2y50]], [[2y6i\|2y6i]], [[2y72\|2y72]], [[4aqo\|4aqo]], [[4ar9\|4ar9]], [[4arf\|4arf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4are FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4are OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4are RCSB], [http://www.ebi.ac.uk/pdbsum/4are PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4are FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4are OCA], [http://pdbe.org/4are PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4are RCSB], [http://www.ebi.ac.uk/pdbsum/4are PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4are ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4are" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Collagenase (non-MMP)\|Collagenase (non-MMP)]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium histolyticum~~]]	+	[[Category: Bacillus histolyticus weinberg and seguin 1916]]
	[[Category: Microbial collagenase]]		[[Category: Microbial collagenase]]
	[[Category: Brandstetter, H]]		[[Category: Brandstetter, H]]

OCA at 09:06, 21 December 2014

2014-12-21T09:06:16Z

←Older revision		Revision as of 09:06, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4are\| PDB=4are \| SCENE= }}~~	+	==Crystal structure of the collagenase Unit of collagenase G from Clostridium histolyticum at 2.19 angstrom resolution.==
-	===Crystal structure of the collagenase Unit of collagenase G from Clostridium histolyticum at 2.19 angstrom resolution.===	+	<StructureSection load='4are' size='340' side='right' caption='[[4are]], [[Resolution\|resolution]] 2.19Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23703618}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4are]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_histolyticum Clostridium histolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ARE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2y3u\|2y3u]], [[2y50\|2y50]], [[2y6i\|2y6i]], [[2y72\|2y72]], [[4aqo\|4aqo]], [[4ar9\|4ar9]], [[4arf\|4arf]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4are FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4are OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4are RCSB], [http://www.ebi.ac.uk/pdbsum/4are PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clostridial collagenases are among the most efficient enzymes to degrade by far the most predominant protein in the biosphere. Here we present crystal structures of the peptidases of three clostridial collagenase isoforms (ColG, ColH and ColT). The comparison of unliganded and liganded structures reveal a quaternary subdomain dynamics. In the unliganded ColH structure this globular dynamics is modulated by an aspartate switch motion that binds to the catalytic zinc. We further identified a calcium binding site in proximity to the catalytic zinc. Both ions are required for full activity, explaining why calcium critically affects the enzymatic activity of clostridial collagenases. Our studies further reveal that loops close to the active site thus serve as characteristic substrate selectivity filter. These elements explain the distinct peptidolytic and collagenolytic activities of these enzymes and provide a rational to engineer collagenases with customized substrate specificity as well as for inhibitor design.

-	~~==About this Structure==~~	+	Structural basis for activity regulation and substrate preference of clostridial collagenases G, H, and T.,Eckhard U, Schonauer E, Brandstetter H J Biol Chem. 2013 May 23. PMID:23703618<ref>PMID:23703618</ref>
-	~~[[4are]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_histolyticum Clostridium histolyticum]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4ARE OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023703618</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Clostridium histolyticum]]		[[Category: Clostridium histolyticum]]
	[[Category: Microbial collagenase]]		[[Category: Microbial collagenase]]
-	[[Category: Brandstetter, H.]]	+	[[Category: Brandstetter, H]]
-	[[Category: Eckhard, U.]]	+	[[Category: Eckhard, U]]
	[[Category: Collagen]]		[[Category: Collagen]]
	[[Category: Collagenolysis]]		[[Category: Collagenolysis]]

OCA at 15:24, 19 June 2013

2013-06-19T15:24:07Z

←Older revision		Revision as of 15:24, 19 June 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4are\| PDB=4are \| SCENE= }}
		+	===Crystal structure of the collagenase Unit of collagenase G from Clostridium histolyticum at 2.19 angstrom resolution.===
		+	{{ABSTRACT_PUBMED_23703618}}

-	~~The entry~~ 4are is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4are]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_histolyticum Clostridium histolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARE OCA].

-	~~Authors~~: ~~Eckhard, U.,~~ Brandstetter, H.	+	==Reference==
-		+	<ref group="xtra">PMID:023703618</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal structure of the collagenase Unit of collagenase G from Clostridium histolyticum at 2.19 angstrom resolution~~.	+	[[Category: Clostridium histolyticum]]
		+	[[Category: Microbial collagenase]]
		+	[[Category: Brandstetter, H.]]
		+	[[Category: Eckhard, U.]]
		+	[[Category: Collagen]]
		+	[[Category: Collagenolysis]]
		+	[[Category: Hydrolase]]
		+	[[Category: Metalloprotease]]
		+	[[Category: Peptidase]]

OCA: Protected "4are" [edit=sysop:move=sysop]

2012-05-02T18:50:15Z

Protected "4are" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:50, 2 May 2012

OCA: New page: '''Unreleased structure''' The entry 4are is ON HOLD until Paper Publication Authors: Eckhard, U., Brandstetter, H. Description: Crystal structure of the collagenase Unit of collagenas...

2012-05-02T18:50:14Z

New page: '''Unreleased structure''' The entry 4are is ON HOLD until Paper Publication Authors: Eckhard, U., Brandstetter, H. Description: Crystal structure of the collagenase Unit of collagenas...

New page

'''Unreleased structure'''

The entry 4are is ON HOLD until Paper Publication

Authors: Eckhard, U., Brandstetter, H.

Description: Crystal structure of the collagenase Unit of collagenase G from Clostridium histolyticum at 2.19 angstrom resolution.