4b1m - Revision history

OCA at 11:41, 20 December 2023

2023-12-20T11:41:13Z

←Older revision		Revision as of 11:41, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4b1m' size='340' side='right'caption='[[4b1m]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='4b1m' size='340' side='right'caption='[[4b1m]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b1m]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B1M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b1m]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B1M FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.1Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[4azz\|4azz]], [[4b1l\|4b1l]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PRD_900051:levanbiose'>PRD_900051</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b1m OCA], [https://pdbe.org/4b1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b1m RCSB], [https://www.ebi.ac.uk/pdbsum/4b1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b1m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b1m OCA], [https://pdbe.org/4b1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b1m RCSB], [https://www.ebi.ac.uk/pdbsum/4b1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b1m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SACC_BACSU SACC_BACSU]] Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose.<ref>PMID:7646030</ref>	+	[https://www.uniprot.org/uniprot/SACC_BACSU SACC_BACSU] Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose.<ref>PMID:7646030</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Fructan beta-fructosidase~~]]	+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Basle, A]]	+	[[Category: Basle A]]
-	[[Category: Countinho~~, P M~~]]	+	[[Category: Countinho PM]]
-	[[Category: Cuskin, F]]	+	[[Category: Cuskin F]]
-	[[Category: Davies~~, G J~~]]	+	[[Category: Davies GJ]]
-	[[Category: Flint~~, J E~~]]	+	[[Category: Flint JE]]
-	[[Category: Gilbert~~, H J~~]]	+	[[Category: Gilbert HJ]]
-	[[Category: Gloster~~, T M~~]]	+	[[Category: Gloster TM]]
-	[[Category: Henrissat, B]]	+	[[Category: Henrissat B]]
-	[[Category: Morland, C]]	+	[[Category: Morland C]]
-	[[Category: Solzehinkin, A]]	+	[[Category: Solzehinkin A]]
-	[[Category: Strazzulli, A]]	+	[[Category: Strazzulli A]]
-	~~[[Category: Cbm66]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:56, 31 August 2022

2022-08-31T06:56:48Z

←Older revision		Revision as of 06:56, 31 August 2022
Line 3:		Line 3:
	<StructureSection load='4b1m' size='340' side='right'caption='[[4b1m]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='4b1m' size='340' side='right'caption='[[4b1m]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b1m]] is a 3 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4B1M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b1m]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B1M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~FRU~~:~~FRUCTOSE~~'>~~FRU~~</scene>, <scene name='pdbligand=NA:~~SODIUM~~+ION'>NA</scene>, <scene name='pdbligand=~~SO4~~:~~SULFATE+ION~~'>~~SO4~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azz\|4azz]], [[4b1l\|4b1l]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4azz\|4azz]], [[4b1l\|4b1l]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4b1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b1m OCA], [~~http~~://pdbe.org/4b1m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4b1m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4b1m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4b1m ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b1m OCA], [https://pdbe.org/4b1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b1m RCSB], [https://www.ebi.ac.uk/pdbsum/4b1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b1m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SACC_BACSU SACC_BACSU]] Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose.<ref>PMID:7646030</ref>	+	[[https://www.uniprot.org/uniprot/SACC_BACSU SACC_BACSU]] Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose.<ref>PMID:7646030</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Fructan beta-fructosidase]]		[[Category: Fructan beta-fructosidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 13:17, 13 March 2019

2019-03-13T13:17:39Z

←Older revision		Revision as of 13:17, 13 March 2019
Line 1:		Line 1:

	==CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS==		==CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS==
-	<StructureSection load='4b1m' size='340' side='right' caption='[[4b1m]], [[Resolution\|resolution]] 1.10Å' scene=''>	+	<StructureSection load='4b1m' size='340' side='right'caption='[[4b1m]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b1m]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B1M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b1m]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B1M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:~~SODIUM+ION~~'>NA</scene>, <scene name='pdbligand=~~SO4~~:~~SULFATE~~+ION'>~~SO4~~</scene>, <scene name='pdbligand=~~FRU~~:~~FRUCTOSE~~'>~~FRU~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azz\|4azz]], [[4b1l\|4b1l]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azz\|4azz]], [[4b1l\|4b1l]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus globigii migula 1900~~]]	+	[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Fructan beta-fructosidase]]		[[Category: Fructan beta-fructosidase]]
		+	[[Category: Large Structures]]
	[[Category: Basle, A]]		[[Category: Basle, A]]
	[[Category: Countinho, P M]]		[[Category: Countinho, P M]]

OCA at 01:42, 5 August 2016

2016-08-05T01:42:11Z

←Older revision		Revision as of 01:42, 5 August 2016
Line 1:		Line 1:
		+
	==CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS==		==CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS==
	<StructureSection load='4b1m' size='340' side='right' caption='[[4b1m]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='4b1m' size='340' side='right' caption='[[4b1m]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b1m]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B1M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b1m]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B1M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azz\|4azz]], [[4b1l\|4b1l]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azz\|4azz]], [[4b1l\|4b1l]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b1m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b1m RCSB], [http://www.ebi.ac.uk/pdbsum/4b1m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b1m OCA], [http://pdbe.org/4b1m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b1m RCSB], [http://www.ebi.ac.uk/pdbsum/4b1m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4b1m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4b1m" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~subtilis~~]]	+	[[Category: Bacillus globigii migula 1900]]
	[[Category: Fructan beta-fructosidase]]		[[Category: Fructan beta-fructosidase]]
	[[Category: Basle, A]]		[[Category: Basle, A]]

OCA at 21:41, 24 December 2014

2014-12-24T21:41:42Z

←Older revision		Revision as of 21:41, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b1m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b1m RCSB], [http://www.ebi.ac.uk/pdbsum/4b1m PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b1m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b1m RCSB], [http://www.ebi.ac.uk/pdbsum/4b1m PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SACC_BACSU SACC_BACSU]] Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose.<ref>PMID:7646030</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 16:54, 9 December 2014

2014-12-09T16:54:11Z

←Older revision		Revision as of 16:54, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4b1m.~~png~~\|~~left~~\|~~200px~~]]	+	==CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS==
		+	<StructureSection load='4b1m' size='340' side='right' caption='[[4b1m]], [[Resolution\|resolution]] 1.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4b1m]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B1M FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4azz\|4azz]], [[4b1l\|4b1l]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b1m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b1m RCSB], [http://www.ebi.ac.uk/pdbsum/4b1m PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Noncatalytic carbohydrate binding modules (CBMs) are components of glycoside hydrolases that attack generally inaccessible substrates. CBMs mediate a two- to fivefold elevation in the activity of endo-acting enzymes, likely through increasing the concentration of the appended enzymes in the vicinity of the substrate. The function of CBMs appended to exo-acting glycoside hydrolases is unclear because their typical endo-binding mode would not fulfill a targeting role. Here we show that the Bacillus subtilis exo-acting beta-fructosidase SacC, which specifically hydrolyses levan, contains the founding member of CBM family 66 (CBM66). The SacC-derived CBM66 (BsCBM66) targets the terminal fructosides of the major fructans found in nature. The crystal structure of BsCBM66 in complex with ligands reveals extensive interactions with the terminal fructose moiety (Fru-3) of levantriose but only limited hydrophobic contacts with Fru-2, explaining why the CBM displays broad specificity. Removal of BsCBM66 from SacC results in a approximately 100-fold reduction in activity against levan. The truncated enzyme functions as a nonspecific beta-fructosidase displaying similar activity against beta-2,1- and beta-2,6-linked fructans and their respective fructooligosaccharides. Conversely, appending BsCBM66 to BT3082, a nonspecific beta-fructosidase from Bacteroides thetaiotaomicron, confers exolevanase activity on the enzyme. We propose that BsCBM66 confers specificity for levan, a branched fructan, through an "avidity" mechanism in which the CBM and the catalytic module target the termini of different branches of the same polysaccharide molecule. This report identifies a unique mechanism by which CBMs modulate enzyme function, and shows how specificity can be tailored by integrating nonspecific catalytic and binding modules into a single enzyme.

-	~~{{STRUCTURE_4b1m\| PDB=4b1m \| SCENE= }}~~	+	How nature can exploit nonspecific catalytic and carbohydrate binding modules to create enzymatic specificity.,Cuskin F, Flint JE, Gloster TM, Morland C, Basle A, Henrissat B, Coutinho PM, Strazzulli A, Solovyova AS, Davies GJ, Gilbert HJ Proc Natl Acad Sci U S A. 2012 Dec 3. PMID:23213210<ref>PMID:23213210</ref>

-	~~===CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23213210}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4b1m]] is a 3 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1M OCA].~~	+	</StructureSection>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Fructan beta-fructosidase]]		[[Category: Fructan beta-fructosidase]]
-	[[Category: Basle, A.]]	+	[[Category: Basle, A]]
-	[[Category: Countinho, P M.]]	+	[[Category: Countinho, P M]]
-	[[Category: Cuskin, F.]]	+	[[Category: Cuskin, F]]
-	[[Category: Davies, G J.]]	+	[[Category: Davies, G J]]
-	[[Category: Flint, J E.]]	+	[[Category: Flint, J E]]
-	[[Category: Gilbert, H J.]]	+	[[Category: Gilbert, H J]]
-	[[Category: Gloster, T M.]]	+	[[Category: Gloster, T M]]
-	[[Category: Henrissat, B.]]	+	[[Category: Henrissat, B]]
-	[[Category: Morland, C.]]	+	[[Category: Morland, C]]
-	[[Category: Solzehinkin, A.]]	+	[[Category: Solzehinkin, A]]
-	[[Category: Strazzulli, A.]]	+	[[Category: Strazzulli, A]]
	[[Category: Cbm66]]		[[Category: Cbm66]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 10:33, 19 December 2012

2012-12-19T10:33:29Z

←Older revision		Revision as of 10:33, 19 December 2012
Line 1:		Line 1:
-	[[Image:4b1m.~~jpg~~\|left\|200px]]	+	[[Image:4b1m.png\|left\|200px]]

	{{STRUCTURE_4b1m\| PDB=4b1m \| SCENE= }}		{{STRUCTURE_4b1m\| PDB=4b1m \| SCENE= }}
Line 5:		Line 5:
	===CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS===		===CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS===

		+	{{ABSTRACT_PUBMED_23213210}}

	==About this Structure==		==About this Structure==

OCA at 21:24, 12 December 2012

2012-12-12T21:24:51Z

←Older revision		Revision as of 21:24, 12 December 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4b1m.jpg\|left\|200px]]

-	~~The entry~~ 4b1m ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4b1m\| PDB=4b1m \| SCENE= }}

-	~~Authors: CUSKIN, F., FLINT, J.E., MORLAND, C., BASLE, A., HENRISSAT, B., COUNTINHO, P.M., STRAZZULLI, A., SOLZEHINKIN, A., DAVIES, G.J., GILBERT, H.J., GLOSTER, T.M.~~	+	===CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS===

-	~~Description~~: ~~CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS~~	+
		+	==About this Structure==
		+	[[4b1m]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B1M OCA].
		+	[[Category: Bacillus subtilis]]
		+	[[Category: Fructan beta-fructosidase]]
		+	[[Category: Basle, A.]]
		+	[[Category: Countinho, P M.]]
		+	[[Category: Cuskin, F.]]
		+	[[Category: Davies, G J.]]
		+	[[Category: Flint, J E.]]
		+	[[Category: Gilbert, H J.]]
		+	[[Category: Gloster, T M.]]
		+	[[Category: Henrissat, B.]]
		+	[[Category: Morland, C.]]
		+	[[Category: Solzehinkin, A.]]
		+	[[Category: Strazzulli, A.]]
		+	[[Category: Cbm66]]
		+	[[Category: Hydrolase]]

OCA at 05:11, 15 August 2012

2012-08-15T05:11:28Z

←Older revision		Revision as of 05:11, 15 August 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4b1m is ON HOLD	+	The entry 4b1m is ON HOLD until Paper Publication

	Authors: CUSKIN, F., FLINT, J.E., MORLAND, C., BASLE, A., HENRISSAT, B., COUNTINHO, P.M., STRAZZULLI, A., SOLZEHINKIN, A., DAVIES, G.J., GILBERT, H.J., GLOSTER, T.M.		Authors: CUSKIN, F., FLINT, J.E., MORLAND, C., BASLE, A., HENRISSAT, B., COUNTINHO, P.M., STRAZZULLI, A., SOLZEHINKIN, A., DAVIES, G.J., GILBERT, H.J., GLOSTER, T.M.

	Description: CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS		Description: CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS

OCA: Protected "4b1m" [edit=sysop:move=sysop]

2012-07-18T04:54:59Z

Protected "4b1m" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:54, 18 July 2012