4b3b - Revision history

OCA at 11:42, 20 December 2023

2023-12-20T11:42:12Z

←Older revision		Revision as of 11:42, 20 December 2023
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	<StructureSection load='4b3b' size='340' side='right'caption='[[4b3b]], [[Resolution\|resolution]] 1.19Å' scene=''>		<StructureSection load='4b3b' size='340' side='right'caption='[[4b3b]], [[Resolution\|resolution]] 1.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b3b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_43587 Atcc 43587~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B3B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b3b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B3B FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.193Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>[[1pzn\|1pzn]], [[4a6p\|4a6p]], [[4a6x\|4a6x]], [[4a74\|4a74]], [[4a7o\|4a7o]], [[4b2i\|4b2i]], [[4b2l\|4b2l]], [[4b2p\|4b2p]], [[4b32\|4b32]], [[4b33\|4b33]], [[4b34\|4b34]], [[4b35\|4b35]], [[4b3c\|4b3c]], [[4b3d\|4b3d]]</~~div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3b OCA], [https://pdbe.org/4b3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b3b RCSB], [https://www.ebi.ac.uk/pdbsum/4b3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b3b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3b OCA], [https://pdbe.org/4b3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b3b RCSB], [https://www.ebi.ac.uk/pdbsum/4b3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b3b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RADA_PYRFU RADA_PYRFU]] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.	+	[https://www.uniprot.org/uniprot/RADA_PYRFU RADA_PYRFU] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 43587~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abell, C]]	+	[[Category: Pyrococcus furiosus]]
-	[[Category: Blundell~~, T L~~]]	+	[[Category: Abell C]]
-	[[Category: Ehebauer~~, M T~~]]	+	[[Category: Blundell TL]]
-	[[Category: Hyvonen, M]]	+	[[Category: Ehebauer MT]]
-	[[Category: Marsh, M]]	+	[[Category: Hyvonen M]]
-	[[Category: Pukala, T]]	+	[[Category: Marsh M]]
-	[[Category: Scott~~, D E~~]]	+	[[Category: Pukala T]]
-	[[Category: Venkitaraman~~, A R]]~~	+	[[Category: Scott DE]]
-	~~[[Category: Humanised]]~~	+	[[Category: Venkitaraman AR]]
-	~~[[Category: Hydrolase-peptide complex]]~~	+
-	~~[[Category: Peptide-binding]]~~	+
-	~~[[Category: Recombinase]]~~	+
-	~~[[Category: Thermostable~~]]	+

OCA at 06:58, 31 August 2022

2022-08-31T06:58:17Z

←Older revision		Revision as of 06:58, 31 August 2022
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	<StructureSection load='4b3b' size='340' side='right'caption='[[4b3b]], [[Resolution\|resolution]] 1.19Å' scene=''>		<StructureSection load='4b3b' size='340' side='right'caption='[[4b3b]], [[Resolution\|resolution]] 1.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b3b]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4B3B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b3b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B3B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pzn\|1pzn]], [[4a6p\|4a6p]], [[4a6x\|4a6x]], [[4a74\|4a74]], [[4a7o\|4a7o]], [[4b2i\|4b2i]], [[4b2l\|4b2l]], [[4b2p\|4b2p]], [[4b32\|4b32]], [[4b33\|4b33]], [[4b34\|4b34]], [[4b35\|4b35]], [[4b3c\|4b3c]], [[4b3d\|4b3d]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1pzn\|1pzn]], [[4a6p\|4a6p]], [[4a6x\|4a6x]], [[4a74\|4a74]], [[4a7o\|4a7o]], [[4b2i\|4b2i]], [[4b2l\|4b2l]], [[4b2p\|4b2p]], [[4b32\|4b32]], [[4b33\|4b33]], [[4b34\|4b34]], [[4b35\|4b35]], [[4b3c\|4b3c]], [[4b3d\|4b3d]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3b OCA], [~~http~~://pdbe.org/4b3b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4b3b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4b3b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4b3b ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3b OCA], [https://pdbe.org/4b3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b3b RCSB], [https://www.ebi.ac.uk/pdbsum/4b3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b3b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RADA_PYRFU RADA_PYRFU]] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.	+	[[https://www.uniprot.org/uniprot/RADA_PYRFU RADA_PYRFU]] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Resolvase\|Resolvase]]	+	*[[Resolvase 3D structures\|Resolvase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:59, 13 March 2019

2019-03-13T13:59:58Z

←Older revision		Revision as of 13:59, 13 March 2019
Line 1:		Line 1:

	==Humanised monomeric RadA in complex with FHTA tetrapeptide==		==Humanised monomeric RadA in complex with FHTA tetrapeptide==
-	<StructureSection load='4b3b' size='340' side='right' caption='[[4b3b]], [[Resolution\|resolution]] 1.19Å' scene=''>	+	<StructureSection load='4b3b' size='340' side='right'caption='[[4b3b]], [[Resolution\|resolution]] 1.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4b3b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3B FirstGlance]. <br>		<table><tr><td colspan='2'>[[4b3b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3B FirstGlance]. <br>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 43587]]		[[Category: Atcc 43587]]
		+	[[Category: Large Structures]]
	[[Category: Abell, C]]		[[Category: Abell, C]]
	[[Category: Blundell, T L]]		[[Category: Blundell, T L]]

OCA at 16:49, 5 August 2016

2016-08-05T16:49:13Z

←Older revision		Revision as of 16:49, 5 August 2016
Line 1:		Line 1:
		+
	==Humanised monomeric RadA in complex with FHTA tetrapeptide==		==Humanised monomeric RadA in complex with FHTA tetrapeptide==
	<StructureSection load='4b3b' size='340' side='right' caption='[[4b3b]], [[Resolution\|resolution]] 1.19Å' scene=''>		<StructureSection load='4b3b' size='340' side='right' caption='[[4b3b]], [[Resolution\|resolution]] 1.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4b3b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrococcus_furiosus Pyrococcus furiosus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4b3b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3B FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pzn\|1pzn]], [[4a6p\|4a6p]], [[4a6x\|4a6x]], [[4a74\|4a74]], [[4a7o\|4a7o]], [[4b2i\|4b2i]], [[4b2l\|4b2l]], [[4b2p\|4b2p]], [[4b32\|4b32]], [[4b33\|4b33]], [[4b34\|4b34]], [[4b35\|4b35]], [[4b3c\|4b3c]], [[4b3d\|4b3d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pzn\|1pzn]], [[4a6p\|4a6p]], [[4a6x\|4a6x]], [[4a74\|4a74]], [[4a7o\|4a7o]], [[4b2i\|4b2i]], [[4b2l\|4b2l]], [[4b2p\|4b2p]], [[4b32\|4b32]], [[4b33\|4b33]], [[4b34\|4b34]], [[4b35\|4b35]], [[4b3c\|4b3c]], [[4b3d\|4b3d]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b3b RCSB], [http://www.ebi.ac.uk/pdbsum/4b3b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3b OCA], [http://pdbe.org/4b3b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b3b RCSB], [http://www.ebi.ac.uk/pdbsum/4b3b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4b3b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4b3b" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Resolvase\|Resolvase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pyrococcus furiosus~~]]	+	[[Category: Atcc 43587]]
	[[Category: Abell, C]]		[[Category: Abell, C]]
	[[Category: Blundell, T L]]		[[Category: Blundell, T L]]

OCA at 20:25, 25 December 2014

2014-12-25T20:25:02Z

←Older revision		Revision as of 20:25, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b3b RCSB], [http://www.ebi.ac.uk/pdbsum/4b3b PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b3b RCSB], [http://www.ebi.ac.uk/pdbsum/4b3b PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RADA_PYRFU RADA_PYRFU]] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 16:14, 9 December 2014

2014-12-09T16:14:58Z

←Older revision		Revision as of 16:14, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4b3b.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Humanised monomeric RadA in complex with FHTA tetrapeptide==
		+	<StructureSection load='4b3b' size='340' side='right' caption='[[4b3b]], [[Resolution\|resolution]] 1.19Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4b3b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3B FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pzn\|1pzn]], [[4a6p\|4a6p]], [[4a6x\|4a6x]], [[4a74\|4a74]], [[4a7o\|4a7o]], [[4b2i\|4b2i]], [[4b2l\|4b2l]], [[4b2p\|4b2p]], [[4b32\|4b32]], [[4b33\|4b33]], [[4b34\|4b34]], [[4b35\|4b35]], [[4b3c\|4b3c]], [[4b3d\|4b3d]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b3b RCSB], [http://www.ebi.ac.uk/pdbsum/4b3b PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ability to identify inhibitors of protein-protein interactions represents a major challenge in modern drug discovery and in the development of tools for chemical biology. In recent years, fragment-based approaches have emerged as a new methodology in drug discovery; however, few examples of small molecules that are active against chemotherapeutic targets have been published. Herein, we describe the fragment-based approach of targeting the interaction between the tumour suppressor BRCA2 and the recombination enzyme RAD51; it makes use of a screening pipeline of biophysical techniques that we expect to be more generally applicable to similar targets. Disruption of this interaction in vivo is hypothesised to give rise to cellular hypersensitivity to radiation and genotoxic drugs. We have used protein engineering to create a monomeric form of RAD51 by humanising a thermostable archaeal orthologue, RadA, and used this protein for fragment screening. The initial fragment hits were thoroughly validated biophysically by isothermal titration calorimetry (ITC) and NMR techniques and observed by X-ray crystallography to bind in a shallow surface pocket that is occupied in the native complex by the side chain of a phenylalanine from the conserved FxxA interaction motif found in BRCA2. This represents the first report of fragments or any small molecule binding at this protein-protein interaction site.

-	~~{{STRUCTURE_4b3b\| PDB=4b3b \| SCENE= }}~~	+	Using a Fragment-Based Approach To Target Protein-Protein Interactions.,Scott DE, Ehebauer MT, Pukala T, Marsh M, Blundell TL, Venkitaraman AR, Abell C, Hyvonen M Chembiochem. 2013 Jan 23. doi: 10.1002/cbic.201200521. PMID:23344974<ref>PMID:23344974</ref>

-	~~===Humanised monomeric RadA in complex with FHTA tetrapeptide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23344974}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4b3b]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3B OCA].~~	+	</StructureSection>
	[[Category: Pyrococcus furiosus]]		[[Category: Pyrococcus furiosus]]
-	[[Category: Abell, C.]]	+	[[Category: Abell, C]]
-	[[Category: Blundell, T L.]]	+	[[Category: Blundell, T L]]
-	[[Category: Ehebauer, M T.]]	+	[[Category: Ehebauer, M T]]
-	[[Category: Hyvonen, M.]]	+	[[Category: Hyvonen, M]]
-	[[Category: Marsh, M.]]	+	[[Category: Marsh, M]]
-	[[Category: Pukala, T.]]	+	[[Category: Pukala, T]]
-	[[Category: Scott, D E.]]	+	[[Category: Scott, D E]]
-	[[Category: Venkitaraman, A R.]]	+	[[Category: Venkitaraman, A R]]
	[[Category: Humanised]]		[[Category: Humanised]]
	[[Category: Hydrolase-peptide complex]]		[[Category: Hydrolase-peptide complex]]

OCA at 14:48, 27 February 2013

2013-02-27T14:48:16Z

←Older revision		Revision as of 14:48, 27 February 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4b3b.jpg\|left\|200px]]

-	~~The entry~~ 4b3b ~~is ON HOLD~~	+	{{STRUCTURE_4b3b\| PDB=4b3b \| SCENE= }}

-	~~Authors: Scott, D.E., Ehebauer, M.T., Pukala, T., Marsh, M., Blundell, T.L., Venkitaraman, A.R., Abell, C., Hyvonen, M.~~	+	===Humanised monomeric RadA in complex with FHTA tetrapeptide===

-	~~Description~~: Humanised ~~monomeric RadA in~~ complex ~~with FHTA tetrapeptide~~	+	{{ABSTRACT_PUBMED_23344974}}
		+
		+	==About this Structure==
		+	[[4b3b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3B OCA].
		+	[[Category: Pyrococcus furiosus]]
		+	[[Category: Abell, C.]]
		+	[[Category: Blundell, T L.]]
		+	[[Category: Ehebauer, M T.]]
		+	[[Category: Hyvonen, M.]]
		+	[[Category: Marsh, M.]]
		+	[[Category: Pukala, T.]]
		+	[[Category: Scott, D E.]]
		+	[[Category: Venkitaraman, A R.]]
		+	[[Category: Humanised]]
		+	[[Category: Hydrolase-peptide complex]]
		+	[[Category: Peptide-binding]]
		+	[[Category: Recombinase]]
		+	[[Category: Thermostable]]

OCA: Protected "4b3b" [edit=sysop:move=sysop]

2012-08-01T05:18:21Z

Protected "4b3b" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 1 August 2012

OCA: New page: '''Unreleased structure''' The entry 4b3b is ON HOLD Authors: Scott, D.E., Ehebauer, M.T., Pukala, T., Marsh, M., Blundell, T.L., Venkitaraman, A.R., Abell, C., Hyvonen, M. Description...

2012-08-01T05:18:20Z

New page: '''Unreleased structure''' The entry 4b3b is ON HOLD Authors: Scott, D.E., Ehebauer, M.T., Pukala, T., Marsh, M., Blundell, T.L., Venkitaraman, A.R., Abell, C., Hyvonen, M. Description...

New page

'''Unreleased structure'''

The entry 4b3b is ON HOLD

Authors: Scott, D.E., Ehebauer, M.T., Pukala, T., Marsh, M., Blundell, T.L., Venkitaraman, A.R., Abell, C., Hyvonen, M.

Description: Humanised monomeric RadA in complex with FHTA tetrapeptide