4bgd - Revision history

OCA at 11:50, 20 December 2023

2023-12-20T11:50:51Z

←Older revision		Revision as of 11:50, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4bgd' size='340' side='right'caption='[[4bgd]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='4bgd' size='340' side='right'caption='[[4bgd]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bgd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BGD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bgd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BGD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bgd OCA], [https://pdbe.org/4bgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bgd RCSB], [https://www.ebi.ac.uk/pdbsum/4bgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bgd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bgd OCA], [https://pdbe.org/4bgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bgd RCSB], [https://www.ebi.ac.uk/pdbsum/4bgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bgd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BRR2_YEAST BRR2_YEAST]] RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.<ref>PMID:19098916</ref> <ref>PMID:23124065</ref> <ref>PMID:19716790</ref> <ref>PMID:19525970</ref> [[https://www.uniprot.org/uniprot/PRP8_YEAST PRP8_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.<ref>PMID:2835658</ref> <ref>PMID:9150140</ref> <ref>PMID:12773561</ref> <ref>PMID:18779563</ref>	+	[https://www.uniprot.org/uniprot/BRR2_YEAST BRR2_YEAST] RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.<ref>PMID:19098916</ref> <ref>PMID:23124065</ref> <ref>PMID:19716790</ref> <ref>PMID:19525970</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~RNA helicase~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Li, J]]	+	[[Category: Li J]]
-	[[Category: Nagai, K]]	+	[[Category: Nagai K]]
-	[[Category: Nguyen~~, T H.D]]~~	+	[[Category: Nguyen THD]]
-	~~[[Category: Retinitis pigmentosa]]~~	+
-	~~[[Category: Rna helicase]]~~	+
-	~~[[Category: Spliceosome]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: U5 snrnp~~]]	+

OCA at 07:12, 31 August 2022

2022-08-31T07:12:45Z

←Older revision		Revision as of 07:12, 31 August 2022
Line 3:		Line 3:
	<StructureSection load='4bgd' size='340' side='right'caption='[[4bgd]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='4bgd' size='340' side='right'caption='[[4bgd]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bgd]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BGD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bgd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BGD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bgd OCA], [~~http~~://pdbe.org/4bgd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bgd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bgd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bgd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bgd OCA], [https://pdbe.org/4bgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bgd RCSB], [https://www.ebi.ac.uk/pdbsum/4bgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bgd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BRR2_YEAST BRR2_YEAST]] RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.<ref>PMID:19098916</ref> <ref>PMID:23124065</ref> <ref>PMID:19716790</ref> <ref>PMID:19525970</ref> [[~~http~~://www.uniprot.org/uniprot/PRP8_YEAST PRP8_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.<ref>PMID:2835658</ref> <ref>PMID:9150140</ref> <ref>PMID:12773561</ref> <ref>PMID:18779563</ref>	+	[[https://www.uniprot.org/uniprot/BRR2_YEAST BRR2_YEAST]] RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.<ref>PMID:19098916</ref> <ref>PMID:23124065</ref> <ref>PMID:19716790</ref> <ref>PMID:19525970</ref> [[https://www.uniprot.org/uniprot/PRP8_YEAST PRP8_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.<ref>PMID:2835658</ref> <ref>PMID:9150140</ref> <ref>PMID:12773561</ref> <ref>PMID:18779563</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Brr2\|Brr2]]	+	*[[Helicase 3D structures\|Helicase 3D structures]]
-	*[[Helicase\|Helicase]]	+	*[[Pre-mRNA splicing factors 3D structures\|Pre-mRNA splicing factors 3D structures]]
-	*[[Pre-mRNA-splicing ~~factor~~\|Pre-mRNA-splicing ~~factor~~]]	+
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:44, 13 March 2019

2019-03-13T13:44:21Z

←Older revision		Revision as of 13:44, 13 March 2019
Line 1:		Line 1:

	==Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8==		==Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8==
-	<StructureSection load='4bgd' size='340' side='right' caption='[[4bgd]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<StructureSection load='4bgd' size='340' side='right'caption='[[4bgd]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bgd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BGD FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bgd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BGD FirstGlance]. <br>
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: RNA helicase]]		[[Category: RNA helicase]]
	[[Category: Li, J]]		[[Category: Li, J]]

OCA at 15:18, 4 August 2016

2016-08-04T15:18:42Z

←Older revision		Revision as of 15:18, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8==		==Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8==
	<StructureSection load='4bgd' size='340' side='right' caption='[[4bgd]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='4bgd' size='340' side='right' caption='[[4bgd]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bgd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BGD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bgd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BGD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bgd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bgd RCSB], [http://www.ebi.ac.uk/pdbsum/4bgd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bgd OCA], [http://pdbe.org/4bgd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bgd RCSB], [http://www.ebi.ac.uk/pdbsum/4bgd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bgd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/PRP8_YEAST PRP8_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.<ref>PMID:2835658</ref> <ref>PMID:9150140</ref> <ref>PMID:12773561</ref> <ref>PMID:18779563</ref>	+	[[http://www.uniprot.org/uniprot/BRR2_YEAST BRR2_YEAST]] RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.<ref>PMID:19098916</ref> <ref>PMID:23124065</ref> <ref>PMID:19716790</ref> <ref>PMID:19525970</ref> [[http://www.uniprot.org/uniprot/PRP8_YEAST PRP8_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.<ref>PMID:2835658</ref> <ref>PMID:9150140</ref> <ref>PMID:12773561</ref> <ref>PMID:18779563</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4bgd" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
	[[Category: RNA helicase]]		[[Category: RNA helicase]]
-	[[Category: Saccharomyces cerevisiae]]
	[[Category: Li, J]]		[[Category: Li, J]]
	[[Category: Nagai, K]]		[[Category: Nagai, K]]

OCA at 16:19, 24 December 2014

2014-12-24T16:19:03Z

←Older revision		Revision as of 16:19, 24 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4bgd\| PDB=4bgd \| SCENE= }}~~	+	==Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8==
-	===Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8===	+	<StructureSection load='4bgd' size='340' side='right' caption='[[4bgd]], [[Resolution\|resolution]] 3.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23727230}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4bgd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BGD FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bgd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bgd RCSB], [http://www.ebi.ac.uk/pdbsum/4bgd PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/PRP8_YEAST PRP8_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.<ref>PMID:2835658</ref> <ref>PMID:9150140</ref> <ref>PMID:12773561</ref> <ref>PMID:18779563</ref>		[[http://www.uniprot.org/uniprot/PRP8_YEAST PRP8_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.<ref>PMID:2835658</ref> <ref>PMID:9150140</ref> <ref>PMID:12773561</ref> <ref>PMID:18779563</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The U5 small nuclear ribonucleoprotein particle (snRNP) helicase Brr2 disrupts the U4/U6 small nuclear RNA (snRNA) duplex and allows U6 snRNA to engage in an intricate RNA network at the active center of the spliceosome. Here, we present the structure of yeast Brr2 in complex with the Jab1/MPN domain of Prp8, which stimulates Brr2 activity. Contrary to previous reports, our crystal structure and mutagenesis data show that the Jab1/MPN domain binds exclusively to the N-terminal helicase cassette. The residues in the Jab1/MPN domain, whose mutations in human Prp8 cause the degenerative eye disease retinitis pigmentosa, are found at or near the interface with Brr2, clarifying its molecular pathology. In the cytoplasm, Prp8 forms a precursor complex with U5 snRNA, seven Sm proteins, Snu114, and Aar2, but after nuclear import, Brr2 replaces Aar2 to form mature U5 snRNP. Our structure explains why Aar2 and Brr2 are mutually exclusive and provides important insights into the assembly of U5 snRNP.
		+
		+	Structural Basis of Brr2-Prp8 Interactions and Implications for U5 snRNP Biogenesis and the Spliceosome Active Site.,Nguyen TH, Li J, Galej WP, Oshikane H, Newman AJ, Nagai K Structure. 2013 May 29. pii: S0969-2126(13)00129-9. doi:, 10.1016/j.str.2013.04.017. PMID:23727230<ref>PMID:23727230</ref>

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4bgd]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4BGD OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:023727230</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Brr2\|Brr2]]
		+	*[[Helicase\|Helicase]]
		+	*[[Pre-mRNA-splicing factor\|Pre-mRNA-splicing factor]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: RNA helicase]]		[[Category: RNA helicase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Li, J.]]	+	[[Category: Li, J]]
-	[[Category: Nagai, K.]]	+	[[Category: Nagai, K]]
-	[[Category: Nguyen, T H.D.]]	+	[[Category: Nguyen, T H.D]]
	[[Category: Retinitis pigmentosa]]		[[Category: Retinitis pigmentosa]]
	[[Category: Rna helicase]]		[[Category: Rna helicase]]

OCA at 16:17, 19 June 2013

2013-06-19T16:17:11Z

←Older revision		Revision as of 16:17, 19 June 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4bgd\| PDB=4bgd \| SCENE= }}
		+	===Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8===
		+	{{ABSTRACT_PUBMED_23727230}}

-	~~The entry 4bgd~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/PRP8_YEAST PRP8_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.<ref>PMID:2835658</ref> <ref>PMID:9150140</ref> <ref>PMID:12773561</ref> <ref>PMID:18779563</ref>

-	~~Authors~~: ~~Nguyen, T~~.H.D.~~, Li, J~~.~~, Nagai, K~~.	+	==About this Structure==
		+	[[4bgd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGD OCA].

-	~~Description~~: ~~Crystal structure of a splicing complex~~	+	==Reference==
		+	<ref group="xtra">PMID:023727230</ref><references group="xtra"/><references/>
		+	[[Category: RNA helicase]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Li, J.]]
		+	[[Category: Nagai, K.]]
		+	[[Category: Nguyen, T H.D.]]
		+	[[Category: Retinitis pigmentosa]]
		+	[[Category: Rna helicase]]
		+	[[Category: Spliceosome]]
		+	[[Category: Transcription]]
		+	[[Category: U5 snrnp]]

OCA at 19:41, 17 April 2013

2013-04-17T19:41:31Z

←Older revision		Revision as of 19:41, 17 April 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4bgd is ON HOLD	+	The entry 4bgd is ON HOLD until Paper Publication

	Authors: Nguyen, T.H.D., Li, J., Nagai, K.		Authors: Nguyen, T.H.D., Li, J., Nagai, K.

	Description: Crystal structure of a splicing complex		Description: Crystal structure of a splicing complex

OCA: Protected "4bgd" [edit=sysop:move=sysop]

2013-04-04T00:38:20Z

Protected "4bgd" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:38, 4 April 2013

OCA: New page: '''Unreleased structure''' The entry 4bgd is ON HOLD Authors: Nguyen, T.H.D., Li, J., Nagai, K. Description: Crystal structure of a splicing complex

2013-04-04T00:38:19Z

New page: '''Unreleased structure''' The entry 4bgd is ON HOLD Authors: Nguyen, T.H.D., Li, J., Nagai, K. Description: Crystal structure of a splicing complex

New page

'''Unreleased structure'''

The entry 4bgd is ON HOLD

Authors: Nguyen, T.H.D., Li, J., Nagai, K.

Description: Crystal structure of a splicing complex