4bpt - Revision history

OCA at 11:56, 20 December 2023

2023-12-20T11:56:26Z

←Older revision		Revision as of 11:56, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='4bpt' size='340' side='right'caption='[[4bpt]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4bpt' size='340' side='right'caption='[[4bpt]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bpt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_33152 Atcc 33152~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BPT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bpt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BPT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bpt OCA], [https://pdbe.org/4bpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bpt RCSB], [https://www.ebi.ac.uk/pdbsum/4bpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bpt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bpt OCA], [https://pdbe.org/4bpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bpt RCSB], [https://www.ebi.ac.uk/pdbsum/4bpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bpt ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5ZS72_LEGPH Q5ZS72_LEGPH]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 33152]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Phenylalanine 4-monooxygenase~~]]	+	[[Category: Legionella pneumophila]]
-	[[Category: Flydal~~, M I~~]]	+	[[Category: Flydal MI]]
-	[[Category: Leiros, H ~~K.S~~]]	+	[[Category: Leiros H-KS]]
-	[[Category: Martinez, A]]	+	[[Category: Martinez A]]
-	~~[[Category: Aggregation]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Pyomelanin synthesis]]~~	+
-	~~[[Category: Thermostability~~]]	+

OCA at 07:21, 31 August 2022

2022-08-31T07:21:23Z

←Older revision		Revision as of 07:21, 31 August 2022
Line 3:		Line 3:
	<StructureSection load='4bpt' size='340' side='right'caption='[[4bpt]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4bpt' size='340' side='right'caption='[[4bpt]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bpt]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BPT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bpt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BPT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bpt OCA], [~~http~~://pdbe.org/4bpt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bpt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bpt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bpt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bpt OCA], [https://pdbe.org/4bpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bpt RCSB], [https://www.ebi.ac.uk/pdbsum/4bpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bpt ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:

	==See Also==		==See Also==
-	*[[~~Hydroxylase~~\|~~Hydroxylase~~]]	+	*[[Hydroxylases 3D structures\|Hydroxylases 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:29, 13 March 2019

2019-03-13T13:29:32Z

←Older revision		Revision as of 13:29, 13 March 2019
Line 1:		Line 1:
		+
	==Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila==		==Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila==
-	<StructureSection load='4bpt' size='340' side='right' caption='[[4bpt]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4bpt' size='340' side='right'caption='[[4bpt]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bpt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BPT FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bpt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BPT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bpt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bpt RCSB], [http://www.ebi.ac.uk/pdbsum/4bpt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bpt OCA], [http://pdbe.org/4bpt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bpt RCSB], [http://www.ebi.ac.uk/pdbsum/4bpt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bpt ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4bpt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[~~Phenylalanine hydroxylase~~\|~~Phenylalanine hydroxylase~~]]	+	*[[Hydroxylase\|Hydroxylase]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 33152]]		[[Category: Atcc 33152]]
		+	[[Category: Large Structures]]
	[[Category: Phenylalanine 4-monooxygenase]]		[[Category: Phenylalanine 4-monooxygenase]]
	[[Category: Flydal, M I]]		[[Category: Flydal, M I]]

OCA at 14:05, 4 January 2015

2015-01-04T14:05:31Z

←Older revision		Revision as of 14:05, 4 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4bpt\| PDB=4bpt \| SCENE= }}~~	+	==Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila==
-	===Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila===	+	<StructureSection load='4bpt' size='340' side='right' caption='[[4bpt]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24251098}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4bpt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BPT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bpt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bpt RCSB], [http://www.ebi.ac.uk/pdbsum/4bpt PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phenylalanine hydroxylase from Legionella pneumophila (lpPAH) has a major functional role in the synthesis of the pigment pyomelanin, which is a potential virulence factor. We present here the crystal structure of lpPAH, which is a dimeric enzyme that shows high thermostability, with a midpoint denaturation temperature of 79 degrees C, and low substrate affinity. The structure revealed a dimerization motif that includes ionic interactions and a hydrophobic core, composed of both beta-structure and a C-terminal region, with the specific residues (P255, P256, Y257 and F258) interacting with the same residues from the adjacent subunit within the dimer. This unique dimerization interface, together with a number of aromatic clusters, appears to contribute to the high thermal stability of lpPAH. The crystal structure also explains the increased aggregation of the enzyme in the presence of salt. Moreover, the low affinity for substrate l-Phe could be explained from three consecutive glycine residues (G181, 182, 183) located at the substrate-binding site. This is the first structure of a dimeric bacterial PAH and provides a framework for interpreting the molecular and kinetic properties of lpPAH and for further investigating the regulation of the enzyme.

-	~~==About this Structure==~~	+	Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila.,Leiros HK, Flydal MI, Martinez A FEBS Open Bio. 2013 Aug 19;3:370-8. doi: 10.1016/j.fob.2013.08.006. eCollection, 2013. PMID:24251098<ref>PMID:24251098</ref>
-	~~[[4bpt]] is a 4 chain structure with sequence~~ from ~~[http://en~~.~~wikipedia~~.~~org/wiki/Atcc_33152 Atcc 33152]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4BPT OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:024251098</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+
		+	==See Also==
		+	*[[Phenylalanine hydroxylase\|Phenylalanine hydroxylase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Atcc 33152]]		[[Category: Atcc 33152]]
	[[Category: Phenylalanine 4-monooxygenase]]		[[Category: Phenylalanine 4-monooxygenase]]
-	[[Category: Flydal, M I.]]	+	[[Category: Flydal, M I]]
-	[[Category: Leiros, H K.S.]]	+	[[Category: Leiros, H K.S]]
-	[[Category: Martinez, A.]]	+	[[Category: Martinez, A]]
	[[Category: Aggregation]]		[[Category: Aggregation]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Pyomelanin synthesis]]		[[Category: Pyomelanin synthesis]]
	[[Category: Thermostability]]		[[Category: Thermostability]]

OCA at 05:41, 29 January 2014

2014-01-29T05:41:03Z

←Older revision		Revision as of 05:41, 29 January 2014
Line 1:		Line 1:
	{{STRUCTURE_4bpt\| PDB=4bpt \| SCENE= }}		{{STRUCTURE_4bpt\| PDB=4bpt \| SCENE= }}
	===Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila===		===Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila===
		+	{{ABSTRACT_PUBMED_24251098}}

	==About this Structure==		==About this Structure==
-	[[4bpt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Legionella_pneumophila Legionella pneumophila~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPT OCA].	+	[[4bpt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPT OCA].
-	[[Category: ~~Legionella pneumophila~~]]	+
		+	==Reference==
		+	<ref group="xtra">PMID:024251098</ref><references group="xtra"/><references/>
		+	[[Category: Atcc 33152]]
	[[Category: Phenylalanine 4-monooxygenase]]		[[Category: Phenylalanine 4-monooxygenase]]
	[[Category: Flydal, M I.]]		[[Category: Flydal, M I.]]

OCA at 08:31, 21 August 2013

2013-08-21T08:31:50Z

←Older revision		Revision as of 08:31, 21 August 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4bpt\| PDB=4bpt \| SCENE= }}
		+	===Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila===

-	~~The entry~~ 4bpt is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4bpt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPT OCA].
-	~~Authors~~: ~~Leiros, H~~.-K.S., Flydal, M.I., Martinez, A.	+	[[Category: Legionella pneumophila]]
-		+	[[Category: Phenylalanine 4-monooxygenase]]
-	~~Description~~: ~~Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila~~	+	[[Category: Flydal, M I.]]
		+	[[Category: Leiros, H K.S.]]
		+	[[Category: Martinez, A.]]
		+	[[Category: Aggregation]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Pyomelanin synthesis]]
		+	[[Category: Thermostability]]

OCA: Protected "4bpt" [edit=sysop:move=sysop]

2013-06-19T10:53:10Z

Protected "4bpt" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:53, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4bpt is ON HOLD Authors: Leiros, H.-K.S., Flydal, M.I., Martinez, A. Description: Structural and thermodynamic insight into phenylalanine hydroxyla...

2013-06-19T10:53:09Z

New page: '''Unreleased structure''' The entry 4bpt is ON HOLD Authors: Leiros, H.-K.S., Flydal, M.I., Martinez, A. Description: Structural and thermodynamic insight into phenylalanine hydroxyla...

New page

'''Unreleased structure'''

The entry 4bpt is ON HOLD

Authors: Leiros, H.-K.S., Flydal, M.I., Martinez, A.

Description: Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila