4bqn - Revision history

OCA at 07:22, 31 August 2022

2022-08-31T07:22:17Z

←Older revision		Revision as of 07:22, 31 August 2022
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	<StructureSection load='4bqn' size='340' side='right'caption='[[4bqn]], [[Resolution\|resolution]] 1.38Å' scene=''>		<StructureSection load='4bqn' size='340' side='right'caption='[[4bqn]], [[Resolution\|resolution]] 1.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bqn]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BQN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bqn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BQN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bqo\|4bqo]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4bqo\|4bqo]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bqn OCA], [~~http~~://pdbe.org/4bqn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bqn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bqn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bqn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bqn OCA], [https://pdbe.org/4bqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bqn RCSB], [https://www.ebi.ac.uk/pdbsum/4bqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bqn ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:08, 13 March 2019

2019-03-13T13:08:59Z

←Older revision		Revision as of 13:08, 13 March 2019
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	==Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein.==		==Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein.==
-	<StructureSection load='4bqn' size='340' side='right' caption='[[4bqn]], [[Resolution\|resolution]] 1.38Å' scene=''>	+	<StructureSection load='4bqn' size='340' side='right'caption='[[4bqn]], [[Resolution\|resolution]] 1.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BQN FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BQN FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Burps]]		[[Category: Burps]]
		+	[[Category: Large Structures]]
	[[Category: Ayres, E]]		[[Category: Ayres, E]]
	[[Category: Harmer, N J]]		[[Category: Harmer, N J]]

OCA at 14:20, 11 August 2016

2016-08-11T14:20:13Z

←Older revision		Revision as of 14:20, 11 August 2016
Line 1:		Line 1:
		+
	==Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein.==		==Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein.==
	<StructureSection load='4bqn' size='340' side='right' caption='[[4bqn]], [[Resolution\|resolution]] 1.38Å' scene=''>		<StructureSection load='4bqn' size='340' side='right' caption='[[4bqn]], [[Resolution\|resolution]] 1.38Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bqo\|4bqo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bqo\|4bqo]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bqn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bqn RCSB], [http://www.ebi.ac.uk/pdbsum/4bqn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bqn OCA], [http://pdbe.org/4bqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bqn RCSB], [http://www.ebi.ac.uk/pdbsum/4bqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bqn ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4bqn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:21, 5 January 2015

2015-01-05T14:21:22Z

←Older revision		Revision as of 14:21, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BQN FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BQN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bqo\|4bqo]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bqo\|4bqo]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bqn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bqn RCSB], [http://www.ebi.ac.uk/pdbsum/4bqn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bqn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bqn RCSB], [http://www.ebi.ac.uk/pdbsum/4bqn PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Burps]]		[[Category: Burps]]
-	[[Category: Ayres, E.]]	+	[[Category: Ayres, E]]
-	[[Category: Harmer, N J.]]	+	[[Category: Harmer, N J]]
-	[[Category: Isupov, M N.]]	+	[[Category: Isupov, M N]]
-	[[Category: Vivoli, M.]]	+	[[Category: Vivoli, M]]
	[[Category: Acetyltransferase]]		[[Category: Acetyltransferase]]
-	[[Category: Melioidosis.]]	+	[[Category: Melioidosis]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 06:49, 13 August 2014

2014-08-13T06:49:14Z

←Older revision		Revision as of 06:49, 13 August 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4bqn\| PDB=4bqn \| SCENE= }}~~	+	==Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein.==
-	===Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein.===	+	<StructureSection load='4bqn' size='340' side='right' caption='[[4bqn]], [[Resolution\|resolution]] 1.38Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4bqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BQN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bqo\|4bqo]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bqn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bqn RCSB], [http://www.ebi.ac.uk/pdbsum/4bqn PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Capsular polysaccharides (CPSs) are protective structures on the surfaces of many Gram-negative bacteria. The principal CPS of the human pathogen and Tier 1 select agent Burkholderia pseudomallei consists of a linear repeat of --3)--2-O-acetyl-6-deoxy-beta-d-manno-heptopyranose-(1-. This CPS is critical to the virulence of this emerging pathogen and represents a key target for the development of novel therapeutics. wcbI is one of several genes in the CPS biosynthetic cluster whose deletion leads to significant attenuation of the pathogen; unlike most others, it has no homologues of known function and no detectable sequence similarity to any protein with an extant structure. Here, the crystal structure of WcbI bound to its proposed product, coenzyme A, is reported at 1.38 A resolution, solved using the halide-soak method with multiple anomalous dispersion. This structure reveals that WcbI incorporates a previously described 100-amino-acid subdomain into a novel, principally helical fold (310 amino acids). This fold adopts a cradle-like structure, with a deep binding pocket for CoA in the loop-rich cradle. Structural analysis and biophysical assays suggest that WcbI functions as an acetyltransferase enzyme, whilst biochemical tests suggest that another functional module might be required to assist its activity in forming the mature B. pseudomallei capsule.

-	~~==About this Structure==~~	+	Structural insights into WcbI, a novel polysaccharide-biosynthesis enzyme.,Vivoli M, Ayres E, Beaumont E, Isupov MN, Harmer NJ IUCrJ. 2013 Oct 18;1(Pt 1):28-38. doi: 10.1107/S205225251302695X. eCollection, 2014 Jan 1. PMID:25075317<ref>PMID:25075317</ref>
-	~~[[4bqn]] is~~ a ~~2 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki~~/~~Burps Burps]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id~~=~~4BQN OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Burps]]		[[Category: Burps]]
	[[Category: Ayres, E.]]		[[Category: Ayres, E.]]

OCA at 15:02, 3 March 2014

2014-03-03T15:02:02Z

←Older revision		Revision as of 15:02, 3 March 2014
Line 3:		Line 3:

	==About this Structure==		==About this Structure==
-	[[4bqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Burkholderia_pseudomallei_k96243 Burkholderia pseudomallei k96243~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQN OCA].	+	[[4bqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQN OCA].
-	[[Category: ~~Burkholderia pseudomallei k96243~~]]	+	[[Category: Burps]]
	[[Category: Ayres, E.]]		[[Category: Ayres, E.]]
	[[Category: Harmer, N J.]]		[[Category: Harmer, N J.]]

OCA at 07:37, 6 November 2013

2013-11-06T07:37:41Z

←Older revision		Revision as of 07:37, 6 November 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4bqn\| PDB=4bqn \| SCENE= }}
		+	===Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein.===

-	~~The entry~~ 4bqn is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4bqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei_k96243 Burkholderia pseudomallei k96243]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQN OCA].
-	~~Authors~~: ~~Vivoli, M.,~~ Ayres, E., Isupov, M., ~~Harmer, N.J~~.	+	[[Category: Burkholderia pseudomallei k96243]]
-		+	[[Category: Ayres, E.]]
-	~~Description~~: ~~Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein~~.	+	[[Category: Harmer, N J.]]
		+	[[Category: Isupov, M N.]]
		+	[[Category: Vivoli, M.]]
		+	[[Category: Acetyltransferase]]
		+	[[Category: Melioidosis.]]
		+	[[Category: Transferase]]

OCA: Protected "4bqn" [edit=sysop:move=sysop]

2013-06-19T10:54:15Z

Protected "4bqn" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:54, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4bqn is ON HOLD until Paper Publication Authors: Vivoli, M., Ayres, E., Isupov, M., Harmer, N.J. Description: Structural insights into WcbI, a nove...

2013-06-19T10:54:12Z

New page: '''Unreleased structure''' The entry 4bqn is ON HOLD until Paper Publication Authors: Vivoli, M., Ayres, E., Isupov, M., Harmer, N.J. Description: Structural insights into WcbI, a nove...

New page

'''Unreleased structure'''

The entry 4bqn is ON HOLD until Paper Publication

Authors: Vivoli, M., Ayres, E., Isupov, M., Harmer, N.J.

Description: Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein.