4bx5 - Revision history

OCA at 11:59, 20 December 2023

2023-12-20T11:59:51Z

←Older revision		Revision as of 11:59, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bx5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BX5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bx5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BX5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.431Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx5 OCA], [https://pdbe.org/4bx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bx5 RCSB], [https://www.ebi.ac.uk/pdbsum/4bx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bx5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx5 OCA], [https://pdbe.org/4bx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bx5 RCSB], [https://www.ebi.ac.uk/pdbsum/4bx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bx5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Streptavidin is one of the most important hubs for molecular biology, either multimerizing biomolecules, bridging one molecule to another, or anchoring to a biotinylated surface/nanoparticle. Streptavidin has the advantage of rapid ultra-stable binding to biotin. However, the ability of streptavidin to bind four biotinylated molecules in a heterogeneous manner is often limiting. Here, we present an efficient approach to isolate streptavidin tetramers with two biotin-binding sites in a precise arrangement, cis or trans. We genetically modified specific subunits with negatively charged tags, refolded a mixture of monomers, and used ion-exchange chromatography to resolve tetramers according to the number and orientation of tags. We solved the crystal structures of cis-divalent streptavidin to 1.4A resolution and trans-divalent streptavidin to 1.6A resolution, validating the isolation strategy and explaining the behavior of the Dead streptavidin variant. cis- and trans-divalent streptavidins retained tetravalent streptavidin's high thermostability and low off-rate. These defined divalent streptavidins enabled us to uncover how streptavidin binding depends on the nature of the biotin ligand. Biotinylated DNA showed strong negative cooperativity of binding to cis-divalent but not trans-divalent streptavidin. A small biotinylated protein bound readily to cis and trans binding sites. We also solved the structure of trans-divalent streptavidin bound to biotin-4-fluorescein, showing how one ligand obstructs binding to an adjacent biotin-binding site. Using a hexaglutamate tag proved a more powerful way to isolate monovalent streptavidin, for ultra-stable labeling without undesired clustering. These forms of streptavidin allow this key hub to be used with a new level of precision, for homogeneous molecular assembly.
		+
		+	Plug-and-Play Pairing via Defined Divalent Streptavidins.,Fairhead M, Krndija D, Lowe ED, Howarth M J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi:, 10.1016/j.jmb.2013.09.016. PMID:24056174<ref>PMID:24056174</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4bx5" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Avidin 3D structures\|Avidin 3D structures]]		*[[Avidin 3D structures\|Avidin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:14, 7 September 2022

2022-09-07T17:14:33Z

←Older revision		Revision as of 17:14, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4bx5' size='340' side='right'caption='[[4bx5]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='4bx5' size='340' side='right'caption='[[4bx5]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4bx5]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1583 As 4.1583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4BX5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4bx5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BX5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bx6\|4bx6]], [[4bx7\|4bx7]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx5 OCA], [https://pdbe.org/4bx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bx5 RCSB], [https://www.ebi.ac.uk/pdbsum/4bx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bx5 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4bx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx5 OCA], [~~http~~://pdbe.org/4bx5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4bx5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4bx5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4bx5 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Streptavidin is one of the most important hubs for molecular biology, either multimerizing biomolecules, bridging one molecule to another, or anchoring to a biotinylated surface/nanoparticle. Streptavidin has the advantage of rapid ultra-stable binding to biotin. However, the ability of streptavidin to bind four biotinylated molecules in a heterogeneous manner is often limiting. Here, we present an efficient approach to isolate streptavidin tetramers with two biotin-binding sites in a precise arrangement, cis or trans. We genetically modified specific subunits with negatively charged tags, refolded a mixture of monomers, and used ion-exchange chromatography to resolve tetramers according to the number and orientation of tags. We solved the crystal structures of cis-divalent streptavidin to 1.4A resolution and trans-divalent streptavidin to 1.6A resolution, validating the isolation strategy and explaining the behavior of the Dead streptavidin variant. cis- and trans-divalent streptavidins retained tetravalent streptavidin's high thermostability and low off-rate. These defined divalent streptavidins enabled us to uncover how streptavidin binding depends on the nature of the biotin ligand. Biotinylated DNA showed strong negative cooperativity of binding to cis-divalent but not trans-divalent streptavidin. A small biotinylated protein bound readily to cis and trans binding sites. We also solved the structure of trans-divalent streptavidin bound to biotin-4-fluorescein, showing how one ligand obstructs binding to an adjacent biotin-binding site. Using a hexaglutamate tag proved a more powerful way to isolate monovalent streptavidin, for ultra-stable labeling without undesired clustering. These forms of streptavidin allow this key hub to be used with a new level of precision, for homogeneous molecular assembly.	+

-	Plug-and-Play Pairing via Defined Divalent Streptavidins.,Fairhead M, Krndija D, Lowe ED, Howarth M J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi:, 10.1016/j.jmb.2013.09.016. PMID:24056174<ref>PMID:24056174</ref>	+	==See Also==
-		+	*[[Avidin 3D structures\|Avidin 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4bx5" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Fairhead, M~~]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: ~~Howarth,~~ M]]	+	[[Category: Fairhead M]]
-	[[Category: ~~Krndija, D~~]]	+	[[Category: Howarth M]]
-	[[Category: ~~Lowe, E~~ D]]	+	[[Category: Krndija D]]
-	[[Category: ~~Avidin]]~~	+	[[Category: Lowe ED]]
-	~~[[Category: Biotin]]~~	+
-	~~[[Category: Biotin-binding protein~~]]	+

OCA at 13:41, 13 March 2019

2019-03-13T13:41:20Z

←Older revision		Revision as of 13:41, 13 March 2019
Line 1:		Line 1:

	==cis-divalent streptavidin==		==cis-divalent streptavidin==
-	<StructureSection load='4bx5' size='340' side='right' caption='[[4bx5]], [[Resolution\|resolution]] 1.43Å' scene=''>	+	<StructureSection load='4bx5' size='340' side='right'caption='[[4bx5]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4bx5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BX5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4bx5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BX5 FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1583]]		[[Category: As 4 1583]]
		+	[[Category: Large Structures]]
	[[Category: Fairhead, M]]		[[Category: Fairhead, M]]
	[[Category: Howarth, M]]		[[Category: Howarth, M]]

OCA at 12:55, 4 August 2016

2016-08-04T12:55:09Z

←Older revision		Revision as of 12:55, 4 August 2016
Line 1:		Line 1:
		+
	==cis-divalent streptavidin==		==cis-divalent streptavidin==
	<StructureSection load='4bx5' size='340' side='right' caption='[[4bx5]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='4bx5' size='340' side='right' caption='[[4bx5]], [[Resolution\|resolution]] 1.43Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bx6\|4bx6]], [[4bx7\|4bx7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bx6\|4bx6]], [[4bx7\|4bx7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bx5 RCSB], [http://www.ebi.ac.uk/pdbsum/4bx5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx5 OCA], [http://pdbe.org/4bx5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bx5 RCSB], [http://www.ebi.ac.uk/pdbsum/4bx5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bx5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4bx5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:45, 24 December 2014

2014-12-24T14:45:31Z

←Older revision		Revision as of 14:45, 24 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4bx5\| PDB~~=4bx5 \| ~~SCENE~~= }}	+	==cis-divalent streptavidin==
-	===~~cis~~-~~divalent streptavidin~~===	+	<StructureSection load='4bx5' size='340' side='right' caption='[[4bx5]], [[Resolution\|resolution]] 1.43Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24056174}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4bx5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BX5 FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4bx6\|4bx6]], [[4bx7\|4bx7]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bx5 RCSB], [http://www.ebi.ac.uk/pdbsum/4bx5 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).		[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Streptavidin is one of the most important hubs for molecular biology, either multimerizing biomolecules, bridging one molecule to another, or anchoring to a biotinylated surface/nanoparticle. Streptavidin has the advantage of rapid ultra-stable binding to biotin. However, the ability of streptavidin to bind four biotinylated molecules in a heterogeneous manner is often limiting. Here, we present an efficient approach to isolate streptavidin tetramers with two biotin-binding sites in a precise arrangement, cis or trans. We genetically modified specific subunits with negatively charged tags, refolded a mixture of monomers, and used ion-exchange chromatography to resolve tetramers according to the number and orientation of tags. We solved the crystal structures of cis-divalent streptavidin to 1.4A resolution and trans-divalent streptavidin to 1.6A resolution, validating the isolation strategy and explaining the behavior of the Dead streptavidin variant. cis- and trans-divalent streptavidins retained tetravalent streptavidin's high thermostability and low off-rate. These defined divalent streptavidins enabled us to uncover how streptavidin binding depends on the nature of the biotin ligand. Biotinylated DNA showed strong negative cooperativity of binding to cis-divalent but not trans-divalent streptavidin. A small biotinylated protein bound readily to cis and trans binding sites. We also solved the structure of trans-divalent streptavidin bound to biotin-4-fluorescein, showing how one ligand obstructs binding to an adjacent biotin-binding site. Using a hexaglutamate tag proved a more powerful way to isolate monovalent streptavidin, for ultra-stable labeling without undesired clustering. These forms of streptavidin allow this key hub to be used with a new level of precision, for homogeneous molecular assembly.

-	~~==About this Structure==~~	+	Plug-and-Play Pairing via Defined Divalent Streptavidins.,Fairhead M, Krndija D, Lowe ED, Howarth M J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi:, 10.1016/j.jmb.2013.09.016. PMID:24056174<ref>PMID:24056174</ref>
-	~~[[4bx5]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/As_4~~.~~1583 As 4.1583]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.~~il/oca-bin/ocashort?id=4BX5 OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024056174</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: As 4 1583]]		[[Category: As 4 1583]]
-	[[Category: Fairhead, M.]]	+	[[Category: Fairhead, M]]
-	[[Category: Howarth, M.]]	+	[[Category: Howarth, M]]
-	[[Category: Krndija, D.]]	+	[[Category: Krndija, D]]
-	[[Category: Lowe, E D.]]	+	[[Category: Lowe, E D]]
	[[Category: Avidin]]		[[Category: Avidin]]
	[[Category: Biotin]]		[[Category: Biotin]]
	[[Category: Biotin-binding protein]]		[[Category: Biotin-binding protein]]

OCA at 06:57, 15 January 2014

2014-01-15T06:57:08Z

←Older revision		Revision as of 06:57, 15 January 2014
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[4bx5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_avidinii Streptomyces avidinii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA].	+	[[4bx5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:024056174</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:024056174</ref><references group="xtra"/><references/>
-	[[Category: ~~Streptomyces avidinii~~]]	+	[[Category: As 4 1583]]
	[[Category: Fairhead, M.]]		[[Category: Fairhead, M.]]
	[[Category: Howarth, M.]]		[[Category: Howarth, M.]]

OCA at 05:14, 10 October 2013

2013-10-10T05:14:00Z

←Older revision		Revision as of 05:14, 10 October 2013
Line 1:		Line 1:
	{{STRUCTURE_4bx5\| PDB=4bx5 \| SCENE= }}		{{STRUCTURE_4bx5\| PDB=4bx5 \| SCENE= }}
	===cis-divalent streptavidin===		===cis-divalent streptavidin===
		+	{{ABSTRACT_PUBMED_24056174}}

	==Function==		==Function==
Line 7:		Line 8:
	==About this Structure==		==About this Structure==
	[[4bx5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA].		[[4bx5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024056174</ref><references group="xtra"/><references/>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: Fairhead, M.]]		[[Category: Fairhead, M.]]

OCA at 08:15, 29 September 2013

2013-09-29T08:15:59Z

←Older revision		Revision as of 08:15, 29 September 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4bx5\| PDB=4bx5 \| SCENE= }}
		+	===cis-divalent streptavidin===

-	The ~~entry 4bx5~~ is ~~ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

-	~~Authors~~: Fairhead, M., Krndija, D., Lowe, E.D.~~, Howarth, M.~~	+	==About this Structure==
-		+	[[4bx5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX5 OCA].
-	~~Description~~: ~~cis~~-~~divalent streptavidin~~	+	[[Category: Streptomyces avidinii]]
		+	[[Category: Fairhead, M.]]
		+	[[Category: Howarth, M.]]
		+	[[Category: Krndija, D.]]
		+	[[Category: Lowe, E D.]]
		+	[[Category: Avidin]]
		+	[[Category: Biotin]]
		+	[[Category: Biotin-binding protein]]

OCA: Protected "4bx5" [edit=sysop:move=sysop]

2013-07-18T04:41:23Z

Protected "4bx5" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:41, 18 July 2013

OCA: New page: '''Unreleased structure''' The entry 4bx5 is ON HOLD until Paper Publication Authors: Fairhead, M., Krndija, D., Lowe, E.D., Howarth, M. Description: cis-divalent streptavidin

2013-07-18T04:41:22Z

New page: '''Unreleased structure''' The entry 4bx5 is ON HOLD until Paper Publication Authors: Fairhead, M., Krndija, D., Lowe, E.D., Howarth, M. Description: cis-divalent streptavidin

New page

'''Unreleased structure'''

The entry 4bx5 is ON HOLD until Paper Publication

Authors: Fairhead, M., Krndija, D., Lowe, E.D., Howarth, M.

Description: cis-divalent streptavidin