4c2u - Revision history

OCA at 12:02, 20 December 2023

2023-12-20T12:02:30Z

←Older revision		Revision as of 12:02, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4c2u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C2U FirstGlance]. <br>		<table><tr><td colspan='2'>[[4c2u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C2U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c2u OCA], [https://pdbe.org/4c2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c2u RCSB], [https://www.ebi.ac.uk/pdbsum/4c2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c2u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c2u OCA], [https://pdbe.org/4c2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c2u RCSB], [https://www.ebi.ac.uk/pdbsum/4c2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c2u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/Q9RTI9_DEIRA Q9RTI9_DEIRA]]	+	[https://www.uniprot.org/uniprot/Q9RTI9_DEIRA Q9RTI9_DEIRA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DNA helicases are responsible for unwinding the duplex DNA, a key step in many biological processes. UvrD is a DNA helicase involved in several DNA repair pathways. We report here crystal structures of Deinococcus radiodurans UvrD (drUvrD) in complex with DNA in different nucleotide-free and bound states. These structures provide us with three distinct snapshots of drUvrD in action and for the first time trap a DNA helicase undergoing a large-scale spiral movement around duplexed DNA. Our structural data also improve our understanding of the molecular mechanisms that regulate DNA unwinding by Superfamily 1A (SF1A) helicases. Our biochemical data reveal that drUvrD is a DNA-stimulated ATPase, can translocate along ssDNA in the 3'-5' direction and shows ATP-dependent 3'-5', and surprisingly also, 5'-3' helicase activity. Interestingly, we find that these translocase and helicase activities of drUvrD are modulated by the ssDNA binding protein. Analysis of drUvrD mutants indicate that the conserved beta-hairpin structure of drUvrD that functions as a separation pin is critical for both drUvrD's 3'-5' and 5'-3' helicase activities, whereas the GIG motif of drUvrD involved in binding to the DNA duplex is essential for the 5'-3' helicase activity only. These special features of drUvrD may reflect its involvement in a wide range of DNA repair processes in vivo.
		+
		+	Structural and Mechanistic Insight into DNA Unwinding by Deinococcus radiodurans UvrD.,Stelter M, Acajjaoui S, McSweeney S, Timmins J PLoS One. 2013 Oct 15;8(10):e77364. doi: 10.1371/journal.pone.0077364. PMID:24143224<ref>PMID:24143224</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4c2u" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Helicase 3D structures\|Helicase 3D structures]]		*[[Helicase 3D structures\|Helicase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:22, 7 September 2022

2022-09-07T17:22:32Z

←Older revision		Revision as of 17:22, 7 September 2022
Line 3:		Line 3:
	<StructureSection load='4c2u' size='340' side='right'caption='[[4c2u]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='4c2u' size='340' side='right'caption='[[4c2u]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4c2u]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus~~ radiodurans~~" raj et al~~. ~~1960~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4C2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4c2u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C2U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4c2t\|4c2t]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c2u OCA], [https://pdbe.org/4c2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c2u RCSB], [https://www.ebi.ac.uk/pdbsum/4c2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c2u ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4c2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c2u OCA], [~~http~~://pdbe.org/4c2u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4c2u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4c2u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4c2u ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[[https://www.uniprot.org/uniprot/Q9RTI9_DEIRA Q9RTI9_DEIRA]]
-	DNA helicases are responsible for unwinding the duplex DNA, a key step in many biological processes. UvrD is a DNA helicase involved in several DNA repair pathways. We report here crystal structures of Deinococcus radiodurans UvrD (drUvrD) in complex with DNA in different nucleotide-free and bound states. These structures provide us with three distinct snapshots of drUvrD in action and for the first time trap a DNA helicase undergoing a large-scale spiral movement around duplexed DNA. Our structural data also improve our understanding of the molecular mechanisms that regulate DNA unwinding by Superfamily 1A (SF1A) helicases. Our biochemical data reveal that drUvrD is a DNA-stimulated ATPase, can translocate along ssDNA in the 3'-5' direction and shows ATP-dependent 3'-5', and surprisingly also, 5'-3' helicase activity. Interestingly, we find that these translocase and helicase activities of drUvrD are modulated by the ssDNA binding protein. Analysis of drUvrD mutants indicate that the conserved beta-hairpin structure of drUvrD that functions as a separation pin is critical for both drUvrD's 3'-5' and 5'-3' helicase activities, whereas the GIG motif of drUvrD involved in binding to the DNA duplex is essential for the 5'-3' helicase activity only. These special features of drUvrD may reflect its involvement in a wide range of DNA repair processes in vivo.	+
-		+
-	~~Structural and Mechanistic Insight into DNA Unwinding by Deinococcus radiodurans UvrD.,Stelter M, Acajjaoui S, McSweeney S, Timmins J PLoS One. 2013 Oct 15;8(10)~~:~~e77364. doi: 10.1371~~/~~journal.pone.0077364. PMID:24143224<ref>PMID:24143224<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 4c2u" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
-	*[[Helicase\|Helicase]]	+	*[[Helicase 3D structures\|Helicase 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Micrococcus~~ radiodurans ~~raj et al. 1960]]~~	+	[[Category: Deinococcus radiodurans]]
-	~~[[Category: DNA helicase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Acajjaoui, S]]	+	[[Category: Synthetic construct]]
-	[[Category: McSweeney, S]]	+	[[Category: Acajjaoui S]]
-	[[Category: Stelter, M]]	+	[[Category: McSweeney S]]
-	[[Category: Timmins, J]]	+	[[Category: Stelter M]]
-	~~[[Category: Dna helicase]]~~	+	[[Category: Timmins J]]
-	~~[[Category: Dna repair]]~~	+
-	~~[[Category: Hydrolase-dna complex]]~~	+
-	~~[[Category: Nucleotide excision repair~~]]	+

OCA at 13:59, 13 March 2019

2019-03-13T13:59:20Z

←Older revision		Revision as of 13:59, 13 March 2019
Line 1:		Line 1:

	==Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, Form 1==		==Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, Form 1==
-	<StructureSection load='4c2u' size='340' side='right' caption='[[4c2u]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='4c2u' size='340' side='right'caption='[[4c2u]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4c2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C2U FirstGlance]. <br>		<table><tr><td colspan='2'>[[4c2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C2U FirstGlance]. <br>
Line 27:		Line 27:
	[[Category: Micrococcus radiodurans raj et al. 1960]]		[[Category: Micrococcus radiodurans raj et al. 1960]]
	[[Category: DNA helicase]]		[[Category: DNA helicase]]
		+	[[Category: Large Structures]]
	[[Category: Acajjaoui, S]]		[[Category: Acajjaoui, S]]
	[[Category: McSweeney, S]]		[[Category: McSweeney, S]]

OCA at 10:49, 4 August 2016

2016-08-04T10:49:46Z

←Older revision		Revision as of 10:49, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, Form 1==		==Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, Form 1==
	<StructureSection load='4c2u' size='340' side='right' caption='[[4c2u]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='4c2u' size='340' side='right' caption='[[4c2u]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4c2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Deinococcus_radiodurans Deinococcus~~ radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4c2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C2U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4c2t\|4c2t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4c2t\|4c2t]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c2u RCSB], [http://www.ebi.ac.uk/pdbsum/4c2u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c2u OCA], [http://pdbe.org/4c2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c2u RCSB], [http://www.ebi.ac.uk/pdbsum/4c2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c2u ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4c2u" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Micrococcus radiodurans raj et al. 1960]]
	[[Category: DNA helicase]]		[[Category: DNA helicase]]
-	[[Category: Deinococcus radiodurans]]
	[[Category: Acajjaoui, S]]		[[Category: Acajjaoui, S]]
	[[Category: McSweeney, S]]		[[Category: McSweeney, S]]

OCA at 10:07, 21 December 2014

2014-12-21T10:07:37Z

←Older revision		Revision as of 10:07, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4c2u\| PDB=4c2u \| SCENE= }}~~	+	==Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, Form 1==
-	===Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, Form 1===	+	<StructureSection load='4c2u' size='340' side='right' caption='[[4c2u]], [[Resolution\|resolution]] 2.55Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24143224}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4c2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C2U FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4c2t\|4c2t]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c2u RCSB], [http://www.ebi.ac.uk/pdbsum/4c2u PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DNA helicases are responsible for unwinding the duplex DNA, a key step in many biological processes. UvrD is a DNA helicase involved in several DNA repair pathways. We report here crystal structures of Deinococcus radiodurans UvrD (drUvrD) in complex with DNA in different nucleotide-free and bound states. These structures provide us with three distinct snapshots of drUvrD in action and for the first time trap a DNA helicase undergoing a large-scale spiral movement around duplexed DNA. Our structural data also improve our understanding of the molecular mechanisms that regulate DNA unwinding by Superfamily 1A (SF1A) helicases. Our biochemical data reveal that drUvrD is a DNA-stimulated ATPase, can translocate along ssDNA in the 3'-5' direction and shows ATP-dependent 3'-5', and surprisingly also, 5'-3' helicase activity. Interestingly, we find that these translocase and helicase activities of drUvrD are modulated by the ssDNA binding protein. Analysis of drUvrD mutants indicate that the conserved beta-hairpin structure of drUvrD that functions as a separation pin is critical for both drUvrD's 3'-5' and 5'-3' helicase activities, whereas the GIG motif of drUvrD involved in binding to the DNA duplex is essential for the 5'-3' helicase activity only. These special features of drUvrD may reflect its involvement in a wide range of DNA repair processes in vivo.

-	~~==About this Structure==~~	+	Structural and Mechanistic Insight into DNA Unwinding by Deinococcus radiodurans UvrD.,Stelter M, Acajjaoui S, McSweeney S, Timmins J PLoS One. 2013 Oct 15;8(10):e77364. doi: 10.1371/journal.pone.0077364. PMID:24143224<ref>PMID:24143224</ref>
-	~~[[4c2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans~~ Deinococcus radiodurans]. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4C2U OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:024143224</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+
		+	==See Also==
		+	*[[Helicase\|Helicase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: DNA helicase]]		[[Category: DNA helicase]]
	[[Category: Deinococcus radiodurans]]		[[Category: Deinococcus radiodurans]]
-	[[Category: Acajjaoui, S.]]	+	[[Category: Acajjaoui, S]]
-	[[Category: McSweeney, S.]]	+	[[Category: McSweeney, S]]
-	[[Category: Stelter, M.]]	+	[[Category: Stelter, M]]
-	[[Category: Timmins, J.]]	+	[[Category: Timmins, J]]
	[[Category: Dna helicase]]		[[Category: Dna helicase]]
	[[Category: Dna repair]]		[[Category: Dna repair]]
	[[Category: Hydrolase-dna complex]]		[[Category: Hydrolase-dna complex]]
	[[Category: Nucleotide excision repair]]		[[Category: Nucleotide excision repair]]

OCA at 09:42, 30 October 2013

2013-10-30T09:42:41Z

←Older revision		Revision as of 09:42, 30 October 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4c2u\| PDB=4c2u \| SCENE= }}
		+	===Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, Form 1===
		+	{{ABSTRACT_PUBMED_24143224}}

-	~~The entry~~ 4c2u is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4c2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C2U OCA].

-	~~Authors~~: ~~Stelter, M.,~~ Acajjaoui, S., McSweeney, S., Timmins, J.	+	==Reference==
-		+	<ref group="xtra">PMID:024143224</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal structure of Deinococcus radiodurans UvrD in~~ complex ~~with DNA, Form 1~~	+	[[Category: DNA helicase]]
		+	[[Category: Deinococcus radiodurans]]
		+	[[Category: Acajjaoui, S.]]
		+	[[Category: McSweeney, S.]]
		+	[[Category: Stelter, M.]]
		+	[[Category: Timmins, J.]]
		+	[[Category: Dna helicase]]
		+	[[Category: Dna repair]]
		+	[[Category: Hydrolase-dna complex]]
		+	[[Category: Nucleotide excision repair]]

OCA: Protected "4c2u" [edit=sysop:move=sysop]

2013-08-28T06:24:08Z

Protected "4c2u" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:24, 28 August 2013

OCA: New page: '''Unreleased structure''' The entry 4c2u is ON HOLD until Paper Publication Authors: Stelter, M., Acajjaoui, S., McSweeney, S., Timmins, J. Description: Crystal structure of Deinococc...

2013-08-28T06:24:06Z

New page: '''Unreleased structure''' The entry 4c2u is ON HOLD until Paper Publication Authors: Stelter, M., Acajjaoui, S., McSweeney, S., Timmins, J. Description: Crystal structure of Deinococc...

New page

'''Unreleased structure'''

The entry 4c2u is ON HOLD until Paper Publication

Authors: Stelter, M., Acajjaoui, S., McSweeney, S., Timmins, J.

Description: Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, Form 1