4cj2 - Revision history

OCA at 12:11, 20 December 2023

2023-12-20T12:11:03Z

←Older revision		Revision as of 12:11, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cj2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CJ2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cj2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CJ2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cj2 OCA], [https://pdbe.org/4cj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cj2 RCSB], [https://www.ebi.ac.uk/pdbsum/4cj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cj2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cj2 OCA], [https://pdbe.org/4cj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cj2 RCSB], [https://www.ebi.ac.uk/pdbsum/4cj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cj2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>	+	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:17, 14 September 2022

2022-09-14T07:17:40Z

←Older revision		Revision as of 07:17, 14 September 2022
Line 3:		Line 3:
	<StructureSection load='4cj2' size='340' side='right'caption='[[4cj2]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='4cj2' size='340' side='right'caption='[[4cj2]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cj2]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_33909 Atcc 33909~~] and [~~http~~://en.wikipedia.org/wiki/~~Gallus_gallus Gallus gallus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CJ2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4CJ2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cj2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CJ2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cj0\|4cj0]], [[4cj1\|4cj1]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cj2 OCA], [https://pdbe.org/4cj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cj2 RCSB], [https://www.ebi.ac.uk/pdbsum/4cj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cj2 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cj2 OCA], [~~http~~://pdbe.org/4cj2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cj2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cj2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cj2 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>	+	[[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 33909]]
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lysozyme~~]]	+	[[Category: Sulfolobus acidocaldarius]]
-	[[Category: Alzari~~, P M~~]]	+	[[Category: Alzari PM]]
-	[[Category: Behar, G]]	+	[[Category: Behar G]]
-	[[Category: Correa, A]]	+	[[Category: Correa A]]
-	[[Category: Mechaly~~, A E~~]]	+	[[Category: Mechaly AE]]
-	[[Category: Mouratou, B]]	+	[[Category: Mouratou B]]
-	[[Category: Obal, G]]	+	[[Category: Obal G]]
-	[[Category: Oppezzo, P]]	+	[[Category: Oppezzo P]]
-	[[Category: Pacheco, S]]	+	[[Category: Pacheco S]]
-	[[Category: Pecorari, F]]	+	[[Category: Pecorari F]]
-	~~[[Category: Glycosidase]]~~	+
-	~~[[Category: Hydrolase-de novo protein complex~~]]	+

OCA at 08:04, 19 February 2020

2020-02-19T08:04:40Z

←Older revision		Revision as of 08:04, 19 February 2020
Line 1:		Line 1:

	==Crystal structure of HEWL in complex with affitin H4==		==Crystal structure of HEWL in complex with affitin H4==
-	<StructureSection load='4cj2' size='340' side='right' caption='[[4cj2]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='4cj2' size='340' side='right'caption='[[4cj2]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cj2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CJ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CJ2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cj2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CJ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CJ2 FirstGlance]. <br>
Line 29:		Line 29:
	[[Category: Atcc 33909]]		[[Category: Atcc 33909]]
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
		+	[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Alzari, P M]]		[[Category: Alzari, P M]]

OCA at 05:41, 16 May 2018

2018-05-16T05:41:27Z

←Older revision		Revision as of 05:41, 16 May 2018
Line 3:		Line 3:
	<StructureSection load='4cj2' size='340' side='right' caption='[[4cj2]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='4cj2' size='340' side='right' caption='[[4cj2]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cj2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CJ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CJ2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cj2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CJ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CJ2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cj0\|4cj0]], [[4cj1\|4cj1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cj0\|4cj0]], [[4cj1\|4cj1]]</td></tr>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4cj2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4cj2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 33909]]
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]

OCA at 08:55, 4 August 2016

2016-08-04T08:55:31Z

←Older revision		Revision as of 08:55, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of HEWL in complex with affitin H4==		==Crystal structure of HEWL in complex with affitin H4==
	<StructureSection load='4cj2' size='340' side='right' caption='[[4cj2]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='4cj2' size='340' side='right' caption='[[4cj2]], [[Resolution\|resolution]] 1.50Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cj0\|4cj0]], [[4cj1\|4cj1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cj0\|4cj0]], [[4cj1\|4cj1]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cj2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cj2 RCSB], [http://www.ebi.ac.uk/pdbsum/4cj2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cj2 OCA], [http://pdbe.org/4cj2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cj2 RCSB], [http://www.ebi.ac.uk/pdbsum/4cj2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cj2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4cj2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:33, 25 December 2014

2014-12-25T07:33:17Z

←Older revision		Revision as of 07:33, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cj2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CJ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CJ2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cj2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CJ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CJ2 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cj0\|4cj0]], [[4cj1\|4cj1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cj0\|4cj0]], [[4cj1\|4cj1]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cj2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cj2 RCSB], [http://www.ebi.ac.uk/pdbsum/4cj2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cj2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cj2 RCSB], [http://www.ebi.ac.uk/pdbsum/4cj2 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins).,Correa A, Pacheco S, Mechaly AE, Obal G, Behar G, Mouratou B, Oppezzo P, Alzari PM, Pecorari F PLoS One. 2014 May 13;9(5):e97438. doi: 10.1371/journal.pone.0097438. eCollection, 2014. PMID:24823716<ref>PMID:24823716</ref>		Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins).,Correa A, Pacheco S, Mechaly AE, Obal G, Behar G, Mouratou B, Oppezzo P, Alzari PM, Pecorari F PLoS One. 2014 May 13;9(5):e97438. doi: 10.1371/journal.pone.0097438. eCollection, 2014. PMID:24823716<ref>PMID:24823716</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 24:
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
-	[[Category: Alzari, P M.]]	+	[[Category: Alzari, P M]]
-	[[Category: Behar, G.]]	+	[[Category: Behar, G]]
-	[[Category: Correa, A.]]	+	[[Category: Correa, A]]
-	[[Category: Mechaly, A E.]]	+	[[Category: Mechaly, A E]]
-	[[Category: Mouratou, B.]]	+	[[Category: Mouratou, B]]
-	[[Category: Obal, G.]]	+	[[Category: Obal, G]]
-	[[Category: Oppezzo, P.]]	+	[[Category: Oppezzo, P]]
-	[[Category: Pacheco, S.]]	+	[[Category: Pacheco, S]]
-	[[Category: Pecorari, F.]]	+	[[Category: Pecorari, F]]
	[[Category: Glycosidase]]		[[Category: Glycosidase]]
	[[Category: Hydrolase-de novo protein complex]]		[[Category: Hydrolase-de novo protein complex]]

OCA at 07:11, 28 May 2014

2014-05-28T07:11:30Z

←Older revision		Revision as of 07:11, 28 May 2014
Line 8:		Line 8:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cj2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cj2 RCSB], [http://www.ebi.ac.uk/pdbsum/4cj2 PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cj2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cj2 RCSB], [http://www.ebi.ac.uk/pdbsum/4cj2 PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glycosidases are associated with various human diseases. The development of efficient and specific inhibitors may provide powerful tools to modulate their activity. However, achieving high selectivity is a major challenge given that glycosidases with different functions can have similar enzymatic mechanisms and active-site architectures. As an alternative approach to small-chemical compounds, proteinaceous inhibitors might provide a better specificity by involving a larger surface area of interaction. We report here the design and characterization of proteinaceous inhibitors that specifically target endoglycosidases representative of the two major mechanistic classes; retaining and inverting glycosidases. These inhibitors consist of artificial affinity proteins, Affitins, selected against the thermophilic CelD from Clostridium thermocellum and lysozyme from hen egg. They were obtained from libraries of Sac7d variants, which involve either the randomization of a surface or the randomization of a surface and an artificially-extended loop. Glycosidase binders exhibited affinities in the nanomolar range with no cross-recognition, with efficient inhibition of lysozyme (Ki = 45 nM) and CelD (Ki = 95 and 111 nM), high expression yields in Escherichia coli, solubility, and thermal stabilities up to 81.1 degrees C. The crystal structures of glycosidase-Affitin complexes validate our library designs. We observed that Affitins prevented substrate access by two modes of binding; covering or penetrating the catalytic site via the extended loop. In addition, Affitins formed salt-bridges with residues essential for enzymatic activity. These results lead us to propose the use of Affitins as versatile selective glycosidase inhibitors and, potentially, as enzymatic inhibitors in general.
		+
		+	Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins).,Correa A, Pacheco S, Mechaly AE, Obal G, Behar G, Mouratou B, Oppezzo P, Alzari PM, Pecorari F PLoS One. 2014 May 13;9(5):e97438. doi: 10.1371/journal.pone.0097438. eCollection, 2014. PMID:24823716<ref>PMID:24823716</ref>
		+
		+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:13, 21 May 2014

2014-05-21T09:13:44Z

←Older revision		Revision as of 09:13, 21 May 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of HEWL in complex with affitin H4==
-		+	<StructureSection load='4cj2' size='340' side='right' caption='[[4cj2]], [[Resolution\|resolution]] 1.50Å' scene=''>
-	~~The entry~~ 4cj2 is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4cj2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CJ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CJ2 FirstGlance]. <br>
-	~~Authors~~: ~~Correa~~, A., ~~Pacheco~~, S., ~~Mechaly, A~~.E., ~~Obal, G~~., Behar, G., Mouratou, B., Oppezzo, P., ~~Alzari, P~~.~~M.,~~ Pecorari, F.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cj0\|4cj0]], [[4cj1\|4cj1]]</td></tr>
-	~~Description~~: ~~Crystal structure of HEWL in~~ complex ~~with affitin H4~~	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cj2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cj2 RCSB], [http://www.ebi.ac.uk/pdbsum/4cj2 PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gallus gallus]]
		+	[[Category: Lysozyme]]
		+	[[Category: Alzari, P M.]]
		+	[[Category: Behar, G.]]
		+	[[Category: Correa, A.]]
		+	[[Category: Mechaly, A E.]]
		+	[[Category: Mouratou, B.]]
		+	[[Category: Obal, G.]]
		+	[[Category: Oppezzo, P.]]
		+	[[Category: Pacheco, S.]]
		+	[[Category: Pecorari, F.]]
		+	[[Category: Glycosidase]]
		+	[[Category: Hydrolase-de novo protein complex]]

OCA at 08:46, 8 January 2014

2014-01-08T08:46:40Z

←Older revision		Revision as of 08:46, 8 January 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4cj2 is ON HOLD ~~until sometime in the future~~	+	The entry 4cj2 is ON HOLD

	Authors: Correa, A., Pacheco, S., Mechaly, A.E., Obal, G., Behar, G., Mouratou, B., Oppezzo, P., Alzari, P.M., Pecorari, F.		Authors: Correa, A., Pacheco, S., Mechaly, A.E., Obal, G., Behar, G., Mouratou, B., Oppezzo, P., Alzari, P.M., Pecorari, F.

	Description: Crystal structure of HEWL in complex with affitin H4		Description: Crystal structure of HEWL in complex with affitin H4

OCA: Protected "4cj2" [edit=sysop:move=sysop]

2013-12-25T09:08:41Z

Protected "4cj2" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:08, 25 December 2013