4d1b - Revision history

OCA at 12:19, 20 December 2023

2023-12-20T12:19:11Z

←Older revision		Revision as of 12:19, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4d1b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbacterium_liquefaciens Microbacterium liquefaciens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2jlo 2jlo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D1B FirstGlance]. <br>		<table><tr><td colspan='2'>[[4d1b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbacterium_liquefaciens Microbacterium liquefaciens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2jlo 2jlo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D1B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5FH:(5S)-5-BENZYLIMIDAZOLIDINE-2,4-DIONE'>5FH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5FH:(5S)-5-BENZYLIMIDAZOLIDINE-2,4-DIONE'>5FH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d1b OCA], [https://pdbe.org/4d1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d1b RCSB], [https://www.ebi.ac.uk/pdbsum/4d1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d1b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d1b OCA], [https://pdbe.org/4d1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d1b RCSB], [https://www.ebi.ac.uk/pdbsum/4d1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d1b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HYUP_MICLQ HYUP_MICLQ]] Nucleobase-proton symporter that mediates the sodium-dependent binding and uptake of 5-aryl-substituted hydantoin compounds (PubMed:16621827, PubMed:24952894). 5-indolyl methyl hydantoin and 5-benzyl hydantoin are the preferred substrates, with selectivity for a hydrophobic substituent in position 5 of hydantoin and for the L isomer over the D isomer (PubMed:16621827, PubMed:24952894).<ref>PMID:16621827</ref> <ref>PMID:24952894</ref> <ref>PMID:16116274</ref>	+	[https://www.uniprot.org/uniprot/HYUP_MICLQ HYUP_MICLQ] Nucleobase-proton symporter that mediates the sodium-dependent binding and uptake of 5-aryl-substituted hydantoin compounds (PubMed:16621827, PubMed:24952894). 5-indolyl methyl hydantoin and 5-benzyl hydantoin are the preferred substrates, with selectivity for a hydrophobic substituent in position 5 of hydantoin and for the L isomer over the D isomer (PubMed:16621827, PubMed:24952894).<ref>PMID:16621827</ref> <ref>PMID:24952894</ref> <ref>PMID:16116274</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:42, 14 September 2022

2022-09-14T07:42:35Z

←Older revision		Revision as of 07:42, 14 September 2022
Line 3:		Line 3:
	<StructureSection load='4d1b' size='340' side='right'caption='[[4d1b]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='4d1b' size='340' side='right'caption='[[4d1b]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4d1b]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"microbacterium_liquefaciens"_orla-jensen_1919 "microbacterium~~ liquefaciens~~" orla-jensen 1919~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2jlo 2jlo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D1B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4D1B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4d1b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbacterium_liquefaciens Microbacterium liquefaciens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2jlo 2jlo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D1B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5FH:(5S)-5-BENZYLIMIDAZOLIDINE-2,4-DIONE'>5FH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5FH:(5S)-5-BENZYLIMIDAZOLIDINE-2,4-DIONE'>5FH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d1a\|4d1a]], [[4d1c\|4d1c]], [[4d1d\|4d1d]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d1b OCA], [https://pdbe.org/4d1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d1b RCSB], [https://www.ebi.ac.uk/pdbsum/4d1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d1b ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4d1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d1b OCA], [~~http~~://pdbe.org/4d1b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4d1b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4d1b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4d1b ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/HYUP_MICLQ HYUP_MICLQ]] Nucleobase-proton symporter that mediates the sodium-dependent binding and uptake of 5-aryl-substituted hydantoin compounds (PubMed:16621827, PubMed:24952894). 5-indolyl methyl hydantoin and 5-benzyl hydantoin are the preferred substrates, with selectivity for a hydrophobic substituent in position 5 of hydantoin and for the L isomer over the D isomer (PubMed:16621827, PubMed:24952894).<ref>PMID:16621827</ref> <ref>PMID:24952894</ref> <ref>PMID:16116274</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Microbacterium liquefaciens orla-jensen 1919]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brueckner, F]]	+	[[Category: Microbacterium liquefaciens]]
-	[[Category: Cameron~~, A D~~]]	+	[[Category: Brueckner F]]
-	[[Category: Drew, D]]	+	[[Category: Cameron AD]]
-	[[Category: Geng, T]]	+	[[Category: Drew D]]
-	[[Category: Henderson~~, P J.F~~]]	+	[[Category: Geng T]]
-	[[Category: Iwata, S]]	+	[[Category: Henderson PJF]]
-	[[Category: Weyand, S]]	+	[[Category: Iwata S]]
-	~~[[Category: Membrane protein transporter]]~~	+	[[Category: Weyand S]]
-	~~[[Category: Occluded state]]~~	+
-	~~[[Category: Substrate-bound]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 08:51, 20 March 2019

2019-03-20T08:51:30Z

←Older revision		Revision as of 08:51, 20 March 2019
Line 1:		Line 1:

	==STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER, IN A CLOSED CONFORMATION WITH BENZYL-HYDANTOIN==		==STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER, IN A CLOSED CONFORMATION WITH BENZYL-HYDANTOIN==
-	<StructureSection load='4d1b' size='340' side='right' caption='[[4d1b]], [[Resolution\|resolution]] 3.80Å' scene=''>	+	<StructureSection load='4d1b' size='340' side='right'caption='[[4d1b]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4d1b]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2jlo 2jlo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D1B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D1B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4d1b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"microbacterium_liquefaciens"_orla-jensen_1919 "microbacterium liquefaciens" orla-jensen 1919]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2jlo 2jlo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D1B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D1B FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5FH:(5S)-5-BENZYLIMIDAZOLIDINE-2,4-DIONE'>5FH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5FH:(5S)-5-BENZYLIMIDAZOLIDINE-2,4-DIONE'>5FH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d1a\|4d1a]], [[4d1c\|4d1c]], [[4d1d\|4d1d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d1a\|4d1a]], [[4d1c\|4d1c]], [[4d1d\|4d1d]]</td></tr>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Microbacterium liquefaciens orla-jensen 1919]]
		+	[[Category: Large Structures]]
	[[Category: Brueckner, F]]		[[Category: Brueckner, F]]
	[[Category: Cameron, A D]]		[[Category: Cameron, A D]]

OCA at 09:01, 11 August 2016

2016-08-11T09:01:54Z

←Older revision		Revision as of 09:01, 11 August 2016
Line 1:		Line 1:
		+
	==STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER, IN A CLOSED CONFORMATION WITH BENZYL-HYDANTOIN==		==STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER, IN A CLOSED CONFORMATION WITH BENZYL-HYDANTOIN==
	<StructureSection load='4d1b' size='340' side='right' caption='[[4d1b]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='4d1b' size='340' side='right' caption='[[4d1b]], [[Resolution\|resolution]] 3.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5FH:(5S)-5-BENZYLIMIDAZOLIDINE-2,4-DIONE'>5FH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5FH:(5S)-5-BENZYLIMIDAZOLIDINE-2,4-DIONE'>5FH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d1a\|4d1a]], [[4d1c\|4d1c]], [[4d1d\|4d1d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d1a\|4d1a]], [[4d1c\|4d1c]], [[4d1d\|4d1d]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d1b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d1b RCSB], [http://www.ebi.ac.uk/pdbsum/4d1b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d1b OCA], [http://pdbe.org/4d1b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d1b RCSB], [http://www.ebi.ac.uk/pdbsum/4d1b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d1b ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4d1b" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:15, 5 January 2015

2015-01-05T14:15:21Z

←Older revision		Revision as of 14:15, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4d1b]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2jlo 2jlo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D1B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D1B FirstGlance]. <br>		<table><tr><td colspan='2'>[[4d1b]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2jlo 2jlo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D1B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D1B FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5FH:(5S)-5-BENZYLIMIDAZOLIDINE-2,4-DIONE'>5FH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5FH:(5S)-5-BENZYLIMIDAZOLIDINE-2,4-DIONE'>5FH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d1a\|4d1a]], [[4d1c\|4d1c]], [[4d1d\|4d1d]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d1a\|4d1a]], [[4d1c\|4d1c]], [[4d1d\|4d1d]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d1b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d1b RCSB], [http://www.ebi.ac.uk/pdbsum/4d1b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d1b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d1b RCSB], [http://www.ebi.ac.uk/pdbsum/4d1b PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Brueckner, F.]]	+	[[Category: Brueckner, F]]
-	[[Category: Cameron, A D.]]	+	[[Category: Cameron, A D]]
-	[[Category: Drew, D.]]	+	[[Category: Drew, D]]
-	[[Category: Geng, T.]]	+	[[Category: Geng, T]]
-	[[Category: Henderson, P J.F.]]	+	[[Category: Henderson, P J.F]]
-	[[Category: Iwata, S.]]	+	[[Category: Iwata, S]]
-	[[Category: Weyand, S.]]	+	[[Category: Weyand, S]]
	[[Category: Membrane protein transporter]]		[[Category: Membrane protein transporter]]
	[[Category: Occluded state]]		[[Category: Occluded state]]
	[[Category: Substrate-bound]]		[[Category: Substrate-bound]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 08:20, 2 July 2014

2014-07-02T08:20:23Z

←Older revision		Revision as of 08:20, 2 July 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER, IN A CLOSED CONFORMATION WITH BENZYL-HYDANTOIN==
		+	<StructureSection load='4d1b' size='340' side='right' caption='[[4d1b]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4d1b]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2jlo 2jlo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D1B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D1B FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5FH:(5S)-5-BENZYLIMIDAZOLIDINE-2,4-DIONE'>5FH</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d1a\|4d1a]], [[4d1c\|4d1c]], [[4d1d\|4d1d]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d1b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d1b RCSB], [http://www.ebi.ac.uk/pdbsum/4d1b PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The hydantoin transporter Mhp1 is a sodium-coupled secondary active transport protein of the nucleobase-cation-symport family and a member of the widespread 5-helix inverted repeat superfamily of transporters. The structure of Mhp1 was previously solved in three different conformations providing insight into the molecular basis of the alternating access mechanism. Here, we elucidate detailed events of substrate binding, through a combination of crystallography, molecular dynamics, site-directed mutagenesis, biochemical/biophysical assays, and the design and synthesis of novel ligands. We show precisely where 5-substituted hydantoin substrates bind in an extended configuration at the interface of the bundle and hash domains. They are recognised through hydrogen bonds to the hydantoin moiety and the complementarity of the 5-substituent for a hydrophobic pocket in the protein. Furthermore, we describe a novel structure of an intermediate state of the protein with the external thin gate locked open by an inhibitor, 5-(2-naphthylmethyl)-L-hydantoin, which becomes a substrate when leucine 363 is changed to an alanine. We deduce the molecular events that underlie acquisition and transport of a ligand by Mhp1.

-	~~The entry 4d1b is ON HOLD until Paper Publication~~	+	Molecular mechanism of ligand recognition by membrane transport protein, Mhp1.,Simmons KJ, Jackson SM, Brueckner F, Patching SG, Beckstein O, Ivanova E, Geng T, Weyand S, Drew D, Lanigan J, Sharples DJ, Sansom MS, Iwata S, Fishwick CW, Johnson AP, Cameron AD, Henderson PJ EMBO J. 2014 Jun 21. pii: e201387557. PMID:24952894<ref>PMID:24952894</ref>

-	~~Authors~~: Brueckner, F., ~~Geng, T~~.~~, Weyand, S.,~~ Drew, D., ~~Iwata, S~~., Henderson, P.J.F., ~~Cameron~~, ~~A.D~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~STRUCTURE OF MHP1, A NUCLEOBASE~~-~~CATION-SYMPORT-1 FAMILY TRANSPORTER, IN A CLOSED CONFORMATION WITH BENZYL-HYDANTOIN~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Brueckner, F.]]
		+	[[Category: Cameron, A D.]]
		+	[[Category: Drew, D.]]
		+	[[Category: Geng, T.]]
		+	[[Category: Henderson, P J.F.]]
		+	[[Category: Iwata, S.]]
		+	[[Category: Weyand, S.]]
		+	[[Category: Membrane protein transporter]]
		+	[[Category: Occluded state]]
		+	[[Category: Substrate-bound]]
		+	[[Category: Transport protein]]

OCA at 07:31, 21 May 2014

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	Authors: Brueckner, F., Geng, T., Weyand, S., Drew, D., Iwata, S., Henderson, P.J.F., Cameron, A.D.		Authors: Brueckner, F., Geng, T., Weyand, S., Drew, D., Iwata, S., Henderson, P.J.F., Cameron, A.D.

	Description: STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER, IN A CLOSED CONFORMATION WITH BENZYL-HYDANTOIN		Description: STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER, IN A CLOSED CONFORMATION WITH BENZYL-HYDANTOIN

OCA: Protected "4d1b" [edit=sysop:move=sysop]

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Protected "4d1b" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:25, 7 May 2014

OCA: New page: '''Unreleased structure''' The entry 4d1b is ON HOLD Authors: Brueckner, F., Geng, T., Weyand, S., Drew, D., Iwata, S., Henderson, P.J.F., Cameron, A.D. Description: STRUCTURE OF MHP1,...

2014-05-07T07:25:09Z

New page: '''Unreleased structure''' The entry 4d1b is ON HOLD Authors: Brueckner, F., Geng, T., Weyand, S., Drew, D., Iwata, S., Henderson, P.J.F., Cameron, A.D. Description: STRUCTURE OF MHP1,...

New page

'''Unreleased structure'''

The entry 4d1b is ON HOLD

Authors: Brueckner, F., Geng, T., Weyand, S., Drew, D., Iwata, S., Henderson, P.J.F., Cameron, A.D.

Description: STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER, IN A CLOSED CONFORMATION WITH BENZYL-HYDANTOIN