4d47 - Revision history

OCA at 12:20, 20 December 2023

2023-12-20T12:20:32Z

←Older revision		Revision as of 12:20, 20 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4d47]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Erwinia_amylovora_ATCC_49946 Erwinia amylovora ATCC 49946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D47 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4d47]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Erwinia_amylovora_ATCC_49946 Erwinia amylovora ATCC 49946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D47 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.77Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d47 OCA], [https://pdbe.org/4d47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d47 RCSB], [https://www.ebi.ac.uk/pdbsum/4d47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d47 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d47 OCA], [https://pdbe.org/4d47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d47 RCSB], [https://www.ebi.ac.uk/pdbsum/4d47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d47 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A0M3KKU6_ERWAE A0A0M3KKU6_ERWAE]]	+	[https://www.uniprot.org/uniprot/A0A0M3KKU6_ERWAE A0A0M3KKU6_ERWAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:46, 14 September 2022

2022-09-14T07:46:18Z

←Older revision		Revision as of 07:46, 14 September 2022
Line 3:		Line 3:
	<StructureSection load='4d47' size='340' side='right'caption='[[4d47]], [[Resolution\|resolution]] 2.77Å' scene=''>		<StructureSection load='4d47' size='340' side='right'caption='[[4d47]], [[Resolution\|resolution]] 2.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4d47]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Erwae Erwae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D47 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4D47 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4d47]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Erwinia_amylovora_ATCC_49946 Erwinia amylovora ATCC 49946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D47 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d48\|4d48]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d47 OCA], [https://pdbe.org/4d47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d47 RCSB], [https://www.ebi.ac.uk/pdbsum/4d47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d47 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4d47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d47 OCA], [~~http~~://pdbe.org/4d47 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4d47 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4d47 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4d47 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A0M3KKU6_ERWAE A0A0M3KKU6_ERWAE]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Erwae~~]]	+	[[Category: Erwinia amylovora ATCC 49946]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Levansucrase]]~~	+	[[Category: Benini S]]
-	[[Category: Benini, S]]	+	[[Category: Caputi L]]
-	[[Category: Caputi, L]]	+	[[Category: Cianci M]]
-	[[Category: Cianci, M]]	+	[[Category: Wuerges J]]
-	[[Category: Wuerges, J]]	+
-	~~[[Category: Fire blight]]~~	+
-	~~[[Category: Five- bladed beta-propeller]]~~	+
-	~~[[Category: Glycoside hydrolase family 68]]~~	+
-	~~[[Category: Sucrose hydrolysis]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:52, 5 February 2020

2020-02-05T09:52:57Z

←Older revision		Revision as of 09:52, 5 February 2020
Line 1:		Line 1:

	==X-ray structure of the levansucrase from Erwinia amylovora==		==X-ray structure of the levansucrase from Erwinia amylovora==
-	<StructureSection load='4d47' size='340' side='right' caption='[[4d47]], [[Resolution\|resolution]] 2.77Å' scene=''>	+	<StructureSection load='4d47' size='340' side='right'caption='[[4d47]], [[Resolution\|resolution]] 2.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4d47]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwae Erwae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D47 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4d47]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwae Erwae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D47 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Erwae]]		[[Category: Erwae]]
		+	[[Category: Large Structures]]
	[[Category: Levansucrase]]		[[Category: Levansucrase]]
	[[Category: Benini, S]]		[[Category: Benini, S]]

OCA at 05:47, 8 March 2018

2018-03-08T05:47:55Z

←Older revision		Revision as of 05:47, 8 March 2018
Line 1:		Line 1:
		+
	==X-ray structure of the levansucrase from Erwinia amylovora==		==X-ray structure of the levansucrase from Erwinia amylovora==
	<StructureSection load='4d47' size='340' side='right' caption='[[4d47]], [[Resolution\|resolution]] 2.77Å' scene=''>		<StructureSection load='4d47' size='340' side='right' caption='[[4d47]], [[Resolution\|resolution]] 2.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4d47]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D47 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4d47]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwae Erwae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D47 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d48\|4d48]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d48\|4d48]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d47 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d47 RCSB], [http://www.ebi.ac.uk/pdbsum/4d47 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d47 OCA], [http://pdbe.org/4d47 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d47 RCSB], [http://www.ebi.ac.uk/pdbsum/4d47 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d47 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4d47" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Erwae]]
	[[Category: Levansucrase]]		[[Category: Levansucrase]]
	[[Category: Benini, S]]		[[Category: Benini, S]]

OCA at 19:58, 5 August 2015

2015-08-05T19:58:40Z

←Older revision		Revision as of 19:58, 5 August 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==X-ray structure of the levansucrase from Erwinia amylovora==
		+	<StructureSection load='4d47' size='340' side='right' caption='[[4d47]], [[Resolution\|resolution]] 2.77Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4d47]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D47 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d48\|4d48]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d47 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d47 RCSB], [http://www.ebi.ac.uk/pdbsum/4d47 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Levansucrases are members of the glycoside hydrolase family and catalyse both the hydrolysis of the substrate sucrose and the transfer of fructosyl units to acceptor molecules. In the presence of sufficient sucrose, this may either lead to the production of fructooligosaccharides or fructose polymers. Aim of this study is to rationalise the differences in the polymerisation properties of bacterial levansucrases and in particular to identify structural features that determine different product spectrum in the levansucrase of the Gram-negative bacterium Erwinia amylovora (Ea Lsc, EC 2.4.1.10) as compared to Gram-positive bacteria such as Bacillus subtilis levansucrase. Ea is an enterobacterial pathogen responsible for the Fire Blight disease in rosaceous plants (e.g., apple and pear) with considerable interest for the agricultural industry. The crystal structure of Ea Lsc was solved at 2.77A resolution and compared to those of other fructosyltransferases from Gram-positive and Gram-negative bacteria. We propose the structural features, determining the different reaction products, to reside in just a few loops at the rim of the active site funnel. Moreover we propose that loop 8 may have a role in product length determination in Gluconacetobacter diazotrophicus LsdA and Microbacterium saccharophilum FFase. The Ea Lsc structure shows for the first time the products of sucrose hydrolysis still bound in the active site.

-	The ~~entry 4d47 is ON HOLD until Paper Publication~~	+	The crystal structure of Erwinia amylovora levansucrase provides a snapshot of the products of sucrose hydrolysis trapped into the active site.,Wuerges J, Caputi L, Cianci M, Boivin S, Meijers R, Benini S J Struct Biol. 2015 Jul 21. pii: S1047-8477(15)30035-6. doi:, 10.1016/j.jsb.2015.07.010. PMID:26208466<ref>PMID:26208466</ref>

-	~~Authors: Wuerges~~, J.~~, Caputi, L~~.~~, Cianci, M., Benini, S~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: X-ray structure of the levansucrase from Erwinia amylovora~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
-	[[Category: ~~Wuerges, J~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Levansucrase]]
	[[Category: Benini, S]]		[[Category: Benini, S]]
		+	[[Category: Caputi, L]]
	[[Category: Cianci, M]]		[[Category: Cianci, M]]
-	[[Category: ~~Caputi~~, L]]	+	[[Category: Wuerges, J]]
		+	[[Category: Fire blight]]
		+	[[Category: Five- bladed beta-propeller]]
		+	[[Category: Glycoside hydrolase family 68]]
		+	[[Category: Sucrose hydrolysis]]
		+	[[Category: Transferase]]

OCA at 13:45, 24 December 2014

2014-12-24T13:45:25Z

←Older revision		Revision as of 13:45, 24 December 2014
Line 6:		Line 6:

	Description: X-ray structure of the levansucrase from Erwinia amylovora		Description: X-ray structure of the levansucrase from Erwinia amylovora
		+	[[Category: Unreleased Structures]]
		+	[[Category: Wuerges, J]]
		+	[[Category: Benini, S]]
		+	[[Category: Cianci, M]]
		+	[[Category: Caputi, L]]

OCA: Protected "4d47" [edit=sysop:move=sysop]

2014-11-05T07:19:59Z

Protected "4d47" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:19, 5 November 2014

OCA: New page: '''Unreleased structure''' The entry 4d47 is ON HOLD until Paper Publication Authors: Wuerges, J., Caputi, L., Cianci, M., Benini, S. Description: X-ray structure of the levansucrase f...

2014-11-05T07:19:57Z

New page: '''Unreleased structure''' The entry 4d47 is ON HOLD until Paper Publication Authors: Wuerges, J., Caputi, L., Cianci, M., Benini, S. Description: X-ray structure of the levansucrase f...

New page

'''Unreleased structure'''

The entry 4d47 is ON HOLD until Paper Publication

Authors: Wuerges, J., Caputi, L., Cianci, M., Benini, S.

Description: X-ray structure of the levansucrase from Erwinia amylovora