4d96 - Revision history

OCA at 13:42, 8 November 2023

2023-11-08T13:42:00Z

←Older revision		Revision as of 13:42, 8 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4d96]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D96 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4d96]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D96 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5PA:N-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-Y-LMETHYL]-1-AMINO-CYCLOPROPANECARBOXYLIC+ACID'>5PA</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.09Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5PA:N-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-Y-LMETHYL]-1-AMINO-CYCLOPROPANECARBOXYLIC+ACID'>5PA</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d96 OCA], [https://pdbe.org/4d96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d96 RCSB], [https://www.ebi.ac.uk/pdbsum/4d96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d96 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d96 OCA], [https://pdbe.org/4d96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d96 RCSB], [https://www.ebi.ac.uk/pdbsum/4d96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d96 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DCYD_SALTY DCYD_SALTY]] Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine.[HAMAP-Rule:MF_01045]	+	[https://www.uniprot.org/uniprot/DCYD_SALTY DCYD_SALTY] Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine.[HAMAP-Rule:MF_01045]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:53, 14 September 2022

2022-09-14T07:53:16Z

←Older revision		Revision as of 07:53, 14 September 2022
Line 1:		Line 1:

	==D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)==		==D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)==
-	<StructureSection load='4d96' size='340' side='right' caption='[[4d96]], [[Resolution\|resolution]] 2.09Å' scene=''>	+	<StructureSection load='4d96' size='340' side='right'caption='[[4d96]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4d96]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D96 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4D96 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4d96]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D96 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5PA:N-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-Y-LMETHYL]-1-AMINO-CYCLOPROPANECARBOXYLIC+ACID'>5PA</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5PA:N-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-Y-LMETHYL]-1-AMINO-CYCLOPROPANECARBOXYLIC+ACID'>5PA</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d8t\|4d8t]], [[4d8u\|4d8u]], [[4d8w\|4d8w]], [[4d92\|4d92]], [[4d97\|4d97]], [[4d99\|4d99]], [[4d9b\|4d9b]], [[4d9c\|4d9c]], [[4d9e\|4d9e]], [[4d9f\|4d9f]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d96 OCA], [https://pdbe.org/4d96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d96 RCSB], [https://www.ebi.ac.uk/pdbsum/4d96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d96 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dcyD, STM1953 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/D-cysteine_desulfhydrase D-cysteine desulfhydrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.15 4.4.1.15] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4d96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d96 OCA], [~~http~~://pdbe.org/4d96 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4d96 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4d96 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4d96 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DCYD_SALTY DCYD_SALTY]] Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine.[HAMAP-Rule:MF_01045]	+	[[https://www.uniprot.org/uniprot/DCYD_SALTY DCYD_SALTY]] Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine.[HAMAP-Rule:MF_01045]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus typhimurium loeffler 1892~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~D-cysteine desulfhydrase~~]]	+	[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-	[[Category: Bharath~~, S R~~]]	+	[[Category: Bharath SR]]
-	[[Category: Murthy~~, M R.N~~]]	+	[[Category: Murthy MRN]]
-	[[Category: Rajesh~~, K H~~]]	+	[[Category: Rajesh KH]]
-	[[Category: Savithri~~, H S~~]]	+	[[Category: Savithri HS]]
-	[[Category: Shveta, B]]	+	[[Category: Shveta B]]
-	~~[[Category: Fold type ii plp-dependent enzyme]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Plp dependent enzyme]]~~	+
-	~~[[Category: Tryptophan synthase beta subunit like family~~]]	+

OCA at 13:36, 11 August 2016

2016-08-11T13:36:06Z

←Older revision		Revision as of 13:36, 11 August 2016
Line 1:		Line 1:
		+
	==D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)==		==D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)==
	<StructureSection load='4d96' size='340' side='right' caption='[[4d96]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='4d96' size='340' side='right' caption='[[4d96]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4d96]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar~~ typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D96 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4d96]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D96 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5PA:N-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-Y-LMETHYL]-1-AMINO-CYCLOPROPANECARBOXYLIC+ACID'>5PA</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5PA:N-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-Y-LMETHYL]-1-AMINO-CYCLOPROPANECARBOXYLIC+ACID'>5PA</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d8t\|4d8t]], [[4d8u\|4d8u]], [[4d8w\|4d8w]], [[4d92\|4d92]], [[4d97\|4d97]], [[4d99\|4d99]], [[4d9b\|4d9b]], [[4d9c\|4d9c]], [[4d9e\|4d9e]], [[4d9f\|4d9f]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d8t\|4d8t]], [[4d8u\|4d8u]], [[4d8w\|4d8w]], [[4d92\|4d92]], [[4d97\|4d97]], [[4d99\|4d99]], [[4d9b\|4d9b]], [[4d9c\|4d9c]], [[4d9e\|4d9e]], [[4d9f\|4d9f]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dcyD, STM1953 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 ~~Salmonella enterica subsp. enterica serovar Typhimurium~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dcyD, STM1953 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/D-cysteine_desulfhydrase D-cysteine desulfhydrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.15 4.4.1.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/D-cysteine_desulfhydrase D-cysteine desulfhydrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.15 4.4.1.15] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d96 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d96 RCSB], [http://www.ebi.ac.uk/pdbsum/4d96 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d96 OCA], [http://pdbe.org/4d96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d96 RCSB], [http://www.ebi.ac.uk/pdbsum/4d96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d96 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DCYD_SALTY DCYD_SALTY]] Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine.[HAMAP-Rule:MF_01045]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4d96" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus typhimurium loeffler 1892]]
	[[Category: D-cysteine desulfhydrase]]		[[Category: D-cysteine desulfhydrase]]
-	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Bharath, S R]]		[[Category: Bharath, S R]]
	[[Category: Murthy, M R.N]]		[[Category: Murthy, M R.N]]

OCA at 13:55, 4 January 2015

2015-01-04T13:55:28Z

←Older revision		Revision as of 13:55, 4 January 2015
Line 1:		Line 1:
-	[[~~Image:~~4d96.~~jpg~~\|~~left~~\|~~200px~~]]	+	==D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)==
		+	<StructureSection load='4d96' size='340' side='right' caption='[[4d96]], [[Resolution\|resolution]] 2.09Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4d96]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D96 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5PA:N-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-Y-LMETHYL]-1-AMINO-CYCLOPROPANECARBOXYLIC+ACID'>5PA</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d8t\|4d8t]], [[4d8u\|4d8u]], [[4d8w\|4d8w]], [[4d92\|4d92]], [[4d97\|4d97]], [[4d99\|4d99]], [[4d9b\|4d9b]], [[4d9c\|4d9c]], [[4d9e\|4d9e]], [[4d9f\|4d9f]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dcyD, STM1953 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 Salmonella enterica subsp. enterica serovar Typhimurium])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/D-cysteine_desulfhydrase D-cysteine desulfhydrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.15 4.4.1.15] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d96 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d96 RCSB], [http://www.ebi.ac.uk/pdbsum/4d96 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Salmonella typhimurium DCyD (StDCyD) is a fold type II pyridoxal 5' phosphate (PLP)-dependent enzyme that catalyzes the degradation of D-Cys to H(2)S and pyruvate. It also efficiently degrades beta-chloro-D-alanine (betaCDA). D-Ser is a poor substrate while the enzyme is inactive with respect to L-Ser and 1-amino-1-carboxy cyclopropane (ACC). Here, we report the X-ray crystal structures of StDCyD and of crystals obtained in the presence of D-Cys, betaCDA, ACC, D-Ser, L-Ser, D-cycloserine (DCS) and L-cycloserine (LCS) at resolutions ranging from 1.7 to 2.6 A. The polypeptide fold of StDCyD consisting of a small domain (residues 48-161) and a large domain (residues 1-47 and 162-328) resembles other fold type II PLP dependent enzymes. The structures obtained in the presence of D-Cys and betaCDA show the product, pyruvate, bound at a site 4.0-6.0 A away from the active site. ACC forms an external aldimine complex while D- and L-Ser bind non-covalently suggesting that the reaction with these ligands is arrested at Calpha proton abstraction and transimination steps, respectively. In the active site of StDCyD cocrystallized with DCS or LCS, electron density for a pyridoxamine phosphate (PMP) was observed. Crystals soaked in cocktail containing these ligands show density for PLP-cycloserine. Spectroscopic observations also suggest formation of PMP by the hydrolysis of cycloserines. Mutational studies suggest that Ser78 and Gln77 are key determinants of enzyme specificity and the phenolate of Tyr287 is responsible for Calpha proton abstraction from D-Cys. Based on these studies, a probable mechanism for the degradation of D-Cys by StDCyD is proposed.

-	<!--	+	Structural and Mutational Studies on Substrate Specificity and Catalysis of Salmonella typhimurium D-Cysteine Desulfhydrase.,Bharath SR, Bisht S, Harijan RK, Savithri HS, Murthy MR PLoS One. 2012;7(5):e36267. Epub 2012 May 4. PMID:22574144<ref>PMID:22574144</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_4d96"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_4d96\| PDB=4d96 \| SCENE= }}~~	+

-	~~===D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_22574144}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22574144 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22574144}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[4d96]] is a 4 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D96 OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022574144<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: D-cysteine desulfhydrase]]		[[Category: D-cysteine desulfhydrase]]
	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
-	[[Category: Bharath, S R.]]	+	[[Category: Bharath, S R]]
-	[[Category: Murthy, M R.N.]]	+	[[Category: Murthy, M R.N]]
-	[[Category: Rajesh, K H.]]	+	[[Category: Rajesh, K H]]
-	[[Category: Savithri, H S.]]	+	[[Category: Savithri, H S]]
-	[[Category: Shveta, B.]]	+	[[Category: Shveta, B]]
	[[Category: Fold type ii plp-dependent enzyme]]		[[Category: Fold type ii plp-dependent enzyme]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Plp dependent enzyme]]		[[Category: Plp dependent enzyme]]
	[[Category: Tryptophan synthase beta subunit like family]]		[[Category: Tryptophan synthase beta subunit like family]]

OCA at 05:53, 30 May 2012

2012-05-30T05:53:03Z

←Older revision		Revision as of 05:53, 30 May 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4d96.jpg\|left\|200px]]

-	The ~~entry 4d96~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4d96", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4d96\| PDB=4d96 \| SCENE= }}

-	~~Authors: Bharath, S.R., Shveta, B., Rajesh, K.H., Savithri, H.S., Murthy, M.R.N.~~	+	===D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)===

-	~~Description~~: D-~~Cysteine desulfhydrase~~ from Salmonella typhimurium ~~complexed with 1~~-~~amino~~-1-~~carboxycyclopropane (ACC)~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22574144}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22574144 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22574144}}
		+
		+	==About this Structure==
		+	[[4d96]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D96 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022574144</ref><references group="xtra"/>
		+	[[Category: D-cysteine desulfhydrase]]
		+	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
		+	[[Category: Bharath, S R.]]
		+	[[Category: Murthy, M R.N.]]
		+	[[Category: Rajesh, K H.]]
		+	[[Category: Savithri, H S.]]
		+	[[Category: Shveta, B.]]
		+	[[Category: Fold type ii plp-dependent enzyme]]
		+	[[Category: Lyase]]
		+	[[Category: Plp dependent enzyme]]
		+	[[Category: Tryptophan synthase beta subunit like family]]

OCA at 06:19, 15 February 2012

2012-02-15T06:19:23Z

←Older revision		Revision as of 06:19, 15 February 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4d96 is ON HOLD	+	The entry 4d96 is ON HOLD until Paper Publication

	Authors: Bharath, S.R., Shveta, B., Rajesh, K.H., Savithri, H.S., Murthy, M.R.N.		Authors: Bharath, S.R., Shveta, B., Rajesh, K.H., Savithri, H.S., Murthy, M.R.N.

	Description: D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)		Description: D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)

OCA at 06:58, 1 February 2012

2012-02-01T06:58:28Z

←Older revision		Revision as of 06:58, 1 February 2012
Line 3:		Line 3:
	The entry 4d96 is ON HOLD		The entry 4d96 is ON HOLD

-	Authors: S.R., ~~Bharath~~ , ~~Bisht~~, ~~Shveta~~ , K.H., ~~Rajesh~~ , H.S.~~, Savithri~~ , Murthy, M.R.N.	+	Authors: Bharath, S.R., Shveta, B., Rajesh, K.H., Savithri, H.S., Murthy, M.R.N.

	Description: D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)		Description: D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)

OCA: Protected "4d96" [edit=sysop:move=sysop]

2012-01-18T10:27:26Z

Protected "4d96" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:27, 18 January 2012

OCA: New page: '''Unreleased structure''' The entry 4d96 is ON HOLD Authors: S.R., Bharath , Bisht, Shveta , K.H., Rajesh , H.S., Savithri , Murthy, M.R.N. Description: D-Cysteine desulfhydrase from ...

2012-01-18T10:27:25Z

New page: '''Unreleased structure''' The entry 4d96 is ON HOLD Authors: S.R., Bharath , Bisht, Shveta , K.H., Rajesh , H.S., Savithri , Murthy, M.R.N. Description: D-Cysteine desulfhydrase from ...

New page

'''Unreleased structure'''

The entry 4d96 is ON HOLD

Authors: S.R., Bharath , Bisht, Shveta , K.H., Rajesh , H.S., Savithri , Murthy, M.R.N.

Description: D-Cysteine desulfhydrase from Salmonella typhimurium complexed with 1-amino-1-carboxycyclopropane (ACC)