4dez - Revision history

OCA at 08:48, 20 March 2024

2024-03-20T08:48:34Z

←Older revision		Revision as of 08:48, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dez]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DEZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dez]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DEZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dez OCA], [https://pdbe.org/4dez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dez RCSB], [https://www.ebi.ac.uk/pdbsum/4dez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dez ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dez OCA], [https://pdbe.org/4dez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dez RCSB], [https://www.ebi.ac.uk/pdbsum/4dez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dez ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0QR77_MYCS2 A0QR77_MYCS2]] Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).[HAMAP-Rule:MF_01113]	+	[https://www.uniprot.org/uniprot/A0QR77_MYCS2 A0QR77_MYCS2] Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).[HAMAP-Rule:MF_01113]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Y-family DNA polymerases (dPols) have evolved to carry out translesion bypass to rescue stalled replication; prokaryotic members of this family also participate in the phenomenon of adaptive mutagenesis to relieve selection pressure imposed by a maladapted environment. In this study, the first structure of a member of this family from a prokaryote has been determined. The structure of MsPolIV, a Y-family dPol from Mycobacterium smegmatis, shows the presence of the characteristic finger, palm and thumb domains. Surprisingly, the electron-density map of the intact protein does not show density for the PAD region that is unique to members of this family. Analysis of the packing of the molecules in the crystals showed the existence of large solvent-filled voids in which the PAD region could be located in multiple conformations. In line with this observation, analytical gel-filtration and dynamic light-scattering studies showed that MsPolIV undergoes significant compaction upon DNA binding. The PAD region is known to insert into the major groove of the substrate DNA and to play a major role in shaping the active site. Comparison with structures of other Y-family dPols shows that in the absence of tertiary contacts between the PAD domain and the other domains this region has the freedom to adopt multiple orientations. This structural attribute of the PAD will allow these enzymes to accommodate the alterations in the width of the DNA double helix that are necessary to achieve translesion bypass and adaptive mutagenesis and will also allow regulation of their activity to prevent adventitious error-prone DNA synthesis.	+

-	The PAD region in the mycobacterial DinB homologue MsPolIV exhibits positional heterogeneity.,Sharma A, Subramanian V, Nair DT Acta Crystallogr D Biol Crystallogr. 2012 Aug;68(Pt 8):960-7. Epub 2012 Jul 17. PMID:22868761<ref>PMID:22868761</ref>	+	==See Also==
-		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4dez" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:15, 21 September 2022

2022-09-21T08:15:15Z

←Older revision		Revision as of 08:15, 21 September 2022
Line 1:		Line 1:

	==Structure of MsDpo4==		==Structure of MsDpo4==
-	<StructureSection load='4dez' size='340' side='right' caption='[[4dez]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='4dez' size='340' side='right'caption='[[4dez]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dez]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Mycs2 Mycs2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DEZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4DEZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dez]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DEZ FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dinB, dinB1, MSMEG_1014, MSMEG_2294 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dez OCA], [https://pdbe.org/4dez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dez RCSB], [https://www.ebi.ac.uk/pdbsum/4dez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dez ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4dez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dez OCA], [~~http~~://pdbe.org/4dez PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4dez RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4dez PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4dez ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/A0QR77_MYCS2 A0QR77_MYCS2]] Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).[HAMAP-Rule:MF_01113]	+	[[https://www.uniprot.org/uniprot/A0QR77_MYCS2 A0QR77_MYCS2]] Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).[HAMAP-Rule:MF_01113]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 4dez" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4dez" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[DNA polymerase\|DNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~DNA-directed DNA polymerase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Mycs2~~]]	+	[[Category: Mycolicibacterium smegmatis MC2 155]]
-	[[Category: Nair~~, D T~~]]	+	[[Category: Nair DT]]
-	[[Category: Sharma, A]]	+	[[Category: Sharma A]]
-	~~[[Category: Dna polymerase]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Y-family~~]]	+

OCA at 09:47, 4 August 2016

2016-08-04T09:47:30Z

←Older revision		Revision as of 09:47, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of MsDpo4==		==Structure of MsDpo4==
	<StructureSection load='4dez' size='340' side='right' caption='[[4dez]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4dez' size='340' side='right' caption='[[4dez]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dez]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_smegmatis Mycobacterium smegmatis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DEZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DEZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dez]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DEZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DEZ FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dinB, dinB1, MSMEG_1014, MSMEG_2294 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1772 Mycobacterium smegmatis~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dinB, dinB1, MSMEG_1014, MSMEG_2294 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dez OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dez RCSB], [http://www.ebi.ac.uk/pdbsum/4dez PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dez OCA], [http://pdbe.org/4dez PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dez RCSB], [http://www.ebi.ac.uk/pdbsum/4dez PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dez ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4dez" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
-	[[Category: ~~Mycobacterium smegmatis~~]]	+	[[Category: Mycs2]]
	[[Category: Nair, D T]]		[[Category: Nair, D T]]
	[[Category: Sharma, A]]		[[Category: Sharma, A]]

OCA at 08:01, 25 December 2014

2014-12-25T08:01:23Z

←Older revision		Revision as of 08:01, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dez]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DEZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DEZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dez]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DEZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DEZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dinB, dinB1, MSMEG_1014, MSMEG_2294 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1772 Mycobacterium smegmatis])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dinB, dinB1, MSMEG_1014, MSMEG_2294 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1772 Mycobacterium smegmatis])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dez OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dez RCSB], [http://www.ebi.ac.uk/pdbsum/4dez PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dez OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dez RCSB], [http://www.ebi.ac.uk/pdbsum/4dez PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0QR77_MYCS2 A0QR77_MYCS2]] Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).[HAMAP-Rule:MF_01113]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 26:
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
	[[Category: Mycobacterium smegmatis]]		[[Category: Mycobacterium smegmatis]]
-	[[Category: Nair, D T.]]	+	[[Category: Nair, D T]]
-	[[Category: Sharma, A.]]	+	[[Category: Sharma, A]]
	[[Category: Dna polymerase]]		[[Category: Dna polymerase]]
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Y-family]]		[[Category: Y-family]]

OCA at 09:10, 11 June 2014

2014-06-11T09:10:35Z

←Older revision		Revision as of 09:10, 11 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4dez.~~jpg~~\|~~left\|200px~~]]	+	==Structure of MsDpo4==
		+	<StructureSection load='4dez' size='340' side='right' caption='[[4dez]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4dez]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DEZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DEZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dinB, dinB1, MSMEG_1014, MSMEG_2294 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1772 Mycobacterium smegmatis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dez OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dez RCSB], [http://www.ebi.ac.uk/pdbsum/4dez PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Y-family DNA polymerases (dPols) have evolved to carry out translesion bypass to rescue stalled replication; prokaryotic members of this family also participate in the phenomenon of adaptive mutagenesis to relieve selection pressure imposed by a maladapted environment. In this study, the first structure of a member of this family from a prokaryote has been determined. The structure of MsPolIV, a Y-family dPol from Mycobacterium smegmatis, shows the presence of the characteristic finger, palm and thumb domains. Surprisingly, the electron-density map of the intact protein does not show density for the PAD region that is unique to members of this family. Analysis of the packing of the molecules in the crystals showed the existence of large solvent-filled voids in which the PAD region could be located in multiple conformations. In line with this observation, analytical gel-filtration and dynamic light-scattering studies showed that MsPolIV undergoes significant compaction upon DNA binding. The PAD region is known to insert into the major groove of the substrate DNA and to play a major role in shaping the active site. Comparison with structures of other Y-family dPols shows that in the absence of tertiary contacts between the PAD domain and the other domains this region has the freedom to adopt multiple orientations. This structural attribute of the PAD will allow these enzymes to accommodate the alterations in the width of the DNA double helix that are necessary to achieve translesion bypass and adaptive mutagenesis and will also allow regulation of their activity to prevent adventitious error-prone DNA synthesis.

-	~~{{STRUCTURE_4dez\| PDB=4dez \| SCENE= }}~~	+	The PAD region in the mycobacterial DinB homologue MsPolIV exhibits positional heterogeneity.,Sharma A, Subramanian V, Nair DT Acta Crystallogr D Biol Crystallogr. 2012 Aug;68(Pt 8):960-7. Epub 2012 Jul 17. PMID:22868761<ref>PMID:22868761</ref>

-	~~===Structure~~ of ~~MsDpo4===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22868761}}~~	+	==See Also==
-		+	*[[DNA polymerase\|DNA polymerase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4dez~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DEZ OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:022868761<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
	[[Category: Mycobacterium smegmatis]]		[[Category: Mycobacterium smegmatis]]

OCA at 05:55, 22 August 2012

2012-08-22T05:55:23Z

←Older revision		Revision as of 05:55, 22 August 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4dez.jpg\|left\|200px]]

-	~~The entry~~ 4dez ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4dez\| PDB=4dez \| SCENE= }}

-	~~Authors: Nair, D.T., Sharma, A.~~	+	===Structure of MsDpo4===

-	~~Description:~~ Structure ~~of MsDpo4~~	+	{{ABSTRACT_PUBMED_22868761}}
		+
		+	==About this Structure==
		+	[[4dez]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DEZ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022868761</ref><references group="xtra"/>
		+	[[Category: DNA-directed DNA polymerase]]
		+	[[Category: Mycobacterium smegmatis]]
		+	[[Category: Nair, D T.]]
		+	[[Category: Sharma, A.]]
		+	[[Category: Dna polymerase]]
		+	[[Category: Transferase]]
		+	[[Category: Y-family]]

OCA at 05:22, 30 May 2012

2012-05-30T05:22:44Z

←Older revision		Revision as of 05:22, 30 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4dez is ON HOLD	+	The entry 4dez is ON HOLD until Paper Publication

	Authors: Nair, D.T., Sharma, A.		Authors: Nair, D.T., Sharma, A.

	Description: Structure of MsDpo4		Description: Structure of MsDpo4

OCA at 06:20, 15 February 2012

2012-02-15T06:20:09Z

←Older revision		Revision as of 06:20, 15 February 2012
Line 3:		Line 3:
	The entry 4dez is ON HOLD		The entry 4dez is ON HOLD

-	Authors: Nair, D. T., Sharma, A.	+	Authors: Nair, D.T., Sharma, A.

-	Description: MsDpo4	+	Description: Structure of MsDpo4

OCA: Protected "4dez" [edit=sysop:move=sysop]

2012-02-01T07:02:06Z

Protected "4dez" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:02, 1 February 2012

OCA: New page: '''Unreleased structure''' The entry 4dez is ON HOLD Authors: Nair, D. T., Sharma, A. Description: MsDpo4

2012-02-01T07:02:05Z

New page: '''Unreleased structure''' The entry 4dez is ON HOLD Authors: Nair, D. T., Sharma, A. Description: MsDpo4

New page

'''Unreleased structure'''

The entry 4dez is ON HOLD

Authors: Nair, D. T., Sharma, A.

Description: MsDpo4