4dkw - Revision history

OCA at 14:37, 14 March 2024

2024-03-14T14:37:53Z

←Older revision		Revision as of 14:37, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dkw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DKW FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dkw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DKW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.02Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dkw OCA], [https://pdbe.org/4dkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dkw RCSB], [https://www.ebi.ac.uk/pdbsum/4dkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dkw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dkw OCA], [https://pdbe.org/4dkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dkw RCSB], [https://www.ebi.ac.uk/pdbsum/4dkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dkw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TERL_BPP22 TERL_BPP22]] Component of the molecular motor that translocates genomic DNA in empty capsid during DNA packaging. Heterodimerizes with small terminase protein to be docked on capsid portal protein. The latter forms a ring in which genomic DNA in translocated into the capsid. May have or induce an endonuclease activity to cleave the genome concatemer after encapsidation (By similarity).	+	[https://www.uniprot.org/uniprot/TERL_BPP22 TERL_BPP22] Component of the molecular motor that translocates genomic DNA in empty capsid during DNA packaging. Heterodimerizes with small terminase protein to be docked on capsid portal protein. The latter forms a ring in which genomic DNA in translocated into the capsid. May have or induce an endonuclease activity to cleave the genome concatemer after encapsidation (By similarity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Packaging of viral genomes into preformed procapsids requires the controlled and synchronized activity of an ATPase and a genome-processing nuclease, both located in the large (L) terminase subunit. In this paper, we have characterized the structure and regulation of bacteriophage P22 L-terminase (gp2). Limited proteolysis reveals a bipartite organization, consisting of an N-terminal ATPase core flexibly connected to a C-terminal nuclease domain. The 2.02 A crystal structure of P22 headful nuclease obtained by in drop proteolysis of full length L-terminase (FL-L-terminase) reveals a central seven-stranded beta-sheet core that harbors two magnesium ions. Modeling studies with DNA suggest the two ions are poised for two-metal ion-dependent catalysis, but the nuclease DNA-binding surface is sterically hindered by a loop-helix (L1-alpha2) motif, which is incompatible with catalysis. Accordingly, the isolated nuclease is completely inactive in vitro, while it exhibits endonucleolytic activity in the context of FL-L-terminase. Deleting the auto-inhibitory L1-alpha2 motif (or just the loop L1) restores nuclease activity to a level comparable to FL-L-terminase. Together, these results suggest that the activity of P22 headful nuclease is regulated by an intramolecular cross-talk with the N-terminal ATPase domain. This cross-talk allows for precise and controlled cleavage of DNA that is essential for genome-packaging.	+
-		+
-	~~Structure of p22 headful packaging nuclease.,Roy A, Cingolani G J Biol Chem. 2012 Jun 19. PMID:22715098<ref>PMID:22715098</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4dkw" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Terminase 3D Structures\|Terminase 3D Structures]]		*[[Terminase 3D Structures\|Terminase 3D Structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:23, 21 September 2022

2022-09-21T08:23:40Z

←Older revision		Revision as of 08:23, 21 September 2022
Line 1:		Line 1:

	==Structure of P22 Large terminase nuclease domain==		==Structure of P22 Large terminase nuclease domain==
-	<StructureSection load='4dkw' size='340' side='right' caption='[[4dkw]], [[Resolution\|resolution]] 2.02Å' scene=''>	+	<StructureSection load='4dkw' size='340' side='right'caption='[[4dkw]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dkw]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bpp22 Bpp22~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4DKW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dkw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DKW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">2, gene 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 BPP22])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dkw OCA], [https://pdbe.org/4dkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dkw RCSB], [https://www.ebi.ac.uk/pdbsum/4dkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dkw ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4dkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dkw OCA], [~~http~~://pdbe.org/4dkw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4dkw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4dkw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4dkw ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TERL_BPP22 TERL_BPP22]] Component of the molecular motor that translocates genomic DNA in empty capsid during DNA packaging. Heterodimerizes with small terminase protein to be docked on capsid portal protein. The latter forms a ring in which genomic DNA in translocated into the capsid. May have or induce an endonuclease activity to cleave the genome concatemer after encapsidation (By similarity).	+	[[https://www.uniprot.org/uniprot/TERL_BPP22 TERL_BPP22]] Component of the molecular motor that translocates genomic DNA in empty capsid during DNA packaging. Heterodimerizes with small terminase protein to be docked on capsid portal protein. The latter forms a ring in which genomic DNA in translocated into the capsid. May have or induce an endonuclease activity to cleave the genome concatemer after encapsidation (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4dkw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4dkw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Terminase 3D Structures\|Terminase 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bpp22~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Cingolani, G~~]]	+	[[Category: Salmonella virus P22]]
-	[[Category: ~~Roy, A~~]]	+	[[Category: Cingolani G]]
-	[[Category: ~~Dna]]~~	+	[[Category: Roy A]]
-	~~[[Category: Dna-packaging]]~~	+
-	~~[[Category: Dna-packaging motor]]~~	+
-	~~[[Category: Endonuclease]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Large terminase]]~~	+
-	~~[[Category: Nuclease fold]]~~	+
-	~~[[Category: Small terminase~~]]	+

OCA at 18:10, 5 August 2016

2016-08-05T18:10:03Z

←Older revision		Revision as of 18:10, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of P22 Large terminase nuclease domain==		==Structure of P22 Large terminase nuclease domain==
	<StructureSection load='4dkw' size='340' side='right' caption='[[4dkw]], [[Resolution\|resolution]] 2.02Å' scene=''>		<StructureSection load='4dkw' size='340' side='right' caption='[[4dkw]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_p22 Enterobacteria phage p22~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DKW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DKW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">2, gene 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 ~~Enterobacteria phage P22~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">2, gene 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 BPP22])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dkw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dkw RCSB], [http://www.ebi.ac.uk/pdbsum/4dkw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dkw OCA], [http://pdbe.org/4dkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dkw RCSB], [http://www.ebi.ac.uk/pdbsum/4dkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dkw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4dkw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage p22~~]]	+	[[Category: Bpp22]]
	[[Category: Cingolani, G]]		[[Category: Cingolani, G]]
	[[Category: Roy, A]]		[[Category: Roy, A]]

OCA at 20:52, 25 December 2014

2014-12-25T20:52:18Z

←Older revision		Revision as of 20:52, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DKW FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DKW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">2, gene 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 Enterobacteria phage P22])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">2, gene 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 Enterobacteria phage P22])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dkw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dkw RCSB], [http://www.ebi.ac.uk/pdbsum/4dkw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dkw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dkw RCSB], [http://www.ebi.ac.uk/pdbsum/4dkw PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TERL_BPP22 TERL_BPP22]] Component of the molecular motor that translocates genomic DNA in empty capsid during DNA packaging. Heterodimerizes with small terminase protein to be docked on capsid portal protein. The latter forms a ring in which genomic DNA in translocated into the capsid. May have or induce an endonuclease activity to cleave the genome concatemer after encapsidation (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage p22]]		[[Category: Enterobacteria phage p22]]
-	[[Category: Cingolani, G.]]	+	[[Category: Cingolani, G]]
-	[[Category: Roy, A.]]	+	[[Category: Roy, A]]
	[[Category: Dna]]		[[Category: Dna]]
	[[Category: Dna-packaging]]		[[Category: Dna-packaging]]

OCA at 08:37, 5 June 2014

2014-06-05T08:37:04Z

←Older revision		Revision as of 08:37, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4dkw.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of P22 Large terminase nuclease domain==
		+	<StructureSection load='4dkw' size='340' side='right' caption='[[4dkw]], [[Resolution\|resolution]] 2.02Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4dkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DKW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">2, gene 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 Enterobacteria phage P22])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dkw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dkw RCSB], [http://www.ebi.ac.uk/pdbsum/4dkw PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Packaging of viral genomes into preformed procapsids requires the controlled and synchronized activity of an ATPase and a genome-processing nuclease, both located in the large (L) terminase subunit. In this paper, we have characterized the structure and regulation of bacteriophage P22 L-terminase (gp2). Limited proteolysis reveals a bipartite organization, consisting of an N-terminal ATPase core flexibly connected to a C-terminal nuclease domain. The 2.02 A crystal structure of P22 headful nuclease obtained by in drop proteolysis of full length L-terminase (FL-L-terminase) reveals a central seven-stranded beta-sheet core that harbors two magnesium ions. Modeling studies with DNA suggest the two ions are poised for two-metal ion-dependent catalysis, but the nuclease DNA-binding surface is sterically hindered by a loop-helix (L1-alpha2) motif, which is incompatible with catalysis. Accordingly, the isolated nuclease is completely inactive in vitro, while it exhibits endonucleolytic activity in the context of FL-L-terminase. Deleting the auto-inhibitory L1-alpha2 motif (or just the loop L1) restores nuclease activity to a level comparable to FL-L-terminase. Together, these results suggest that the activity of P22 headful nuclease is regulated by an intramolecular cross-talk with the N-terminal ATPase domain. This cross-talk allows for precise and controlled cleavage of DNA that is essential for genome-packaging.

-	~~{{STRUCTURE_4dkw\| PDB=4dkw \| SCENE= }}~~	+	Structure of p22 headful packaging nuclease.,Roy A, Cingolani G J Biol Chem. 2012 Jun 19. PMID:22715098<ref>PMID:22715098</ref>

-	~~===Structure~~ of ~~P22 Large terminase nuclease domain===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22715098}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[4dkw]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022715098<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Enterobacteria phage p22]]		[[Category: Enterobacteria phage p22]]
	[[Category: Cingolani, G.]]		[[Category: Cingolani, G.]]

OCA at 06:56, 4 July 2012

2012-07-04T06:56:58Z

←Older revision		Revision as of 06:56, 4 July 2012
Line 1:		Line 1:
-	[[Image:4dkw.~~jpg~~\|left\|200px]]	+	[[Image:4dkw.png\|left\|200px]]

	{{STRUCTURE_4dkw\| PDB=4dkw \| SCENE= }}		{{STRUCTURE_4dkw\| PDB=4dkw \| SCENE= }}
Line 5:		Line 5:
	===Structure of P22 Large terminase nuclease domain===		===Structure of P22 Large terminase nuclease domain===

		+	{{ABSTRACT_PUBMED_22715098}}

	==About this Structure==		==About this Structure==
	[[4dkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA].		[[4dkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022715098</ref><references group="xtra"/>
	[[Category: Enterobacteria phage p22]]		[[Category: Enterobacteria phage p22]]
	[[Category: Cingolani, G.]]		[[Category: Cingolani, G.]]

OCA at 05:56, 27 June 2012

2012-06-27T05:56:48Z

←Older revision		Revision as of 05:56, 27 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4dkw.jpg\|left\|200px]]

-	~~The entry~~ 4dkw ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4dkw\| PDB=4dkw \| SCENE= }}

-	~~Authors: Roy, A., Cingolani, G.~~	+	===Structure of P22 Large terminase nuclease domain===

-	~~Description:~~ Structure ~~of P22~~ Large terminase ~~nuclease domain~~	+
		+	==About this Structure==
		+	[[4dkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKW OCA].
		+	[[Category: Enterobacteria phage p22]]
		+	[[Category: Cingolani, G.]]
		+	[[Category: Roy, A.]]
		+	[[Category: Dna]]
		+	[[Category: Dna-packaging]]
		+	[[Category: Dna-packaging motor]]
		+	[[Category: Endonuclease]]
		+	[[Category: Hydrolase]]
		+	[[Category: Large terminase]]
		+	[[Category: Nuclease fold]]
		+	[[Category: Small terminase]]

OCA at 05:24, 8 March 2012

2012-03-08T05:24:22Z

←Older revision		Revision as of 05:24, 8 March 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4dkw is ON HOLD	+	The entry 4dkw is ON HOLD until Paper Publication

	Authors: Roy, A., Cingolani, G.		Authors: Roy, A., Cingolani, G.

	Description: Structure of P22 Large terminase nuclease domain		Description: Structure of P22 Large terminase nuclease domain

OCA: Protected "4dkw" [edit=sysop:move=sysop]

2012-02-15T06:22:44Z

Protected "4dkw" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:22, 15 February 2012

OCA: New page: '''Unreleased structure''' The entry 4dkw is ON HOLD Authors: Roy, A., Cingolani, G. Description: Structure of P22 Large terminase nuclease domain

2012-02-15T06:22:43Z

New page: '''Unreleased structure''' The entry 4dkw is ON HOLD Authors: Roy, A., Cingolani, G. Description: Structure of P22 Large terminase nuclease domain

New page

'''Unreleased structure'''

The entry 4dkw is ON HOLD

Authors: Roy, A., Cingolani, G.

Description: Structure of P22 Large terminase nuclease domain