4ee7 - Revision history

OCA at 07:07, 28 September 2022

2022-09-28T07:07:20Z

←Older revision		Revision as of 07:07, 28 September 2022
Line 1:		Line 1:

	==Crystal Structure of the Novel Phenazine Prenyltransferase EpzP in complex with S-thiolodiphosphate (methylated)==		==Crystal Structure of the Novel Phenazine Prenyltransferase EpzP in complex with S-thiolodiphosphate (methylated)==
-	<StructureSection load='4ee7' size='340' side='right' caption='[[4ee7]], [[Resolution\|resolution]] 1.67Å' scene=''>	+	<StructureSection load='4ee7' size='340' side='right'caption='[[4ee7]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ee7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1619 As 4.1619~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EE7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ee7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cinnamonensis Streptomyces cinnamonensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EE7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=~~PG4~~:~~TETRAETHYLENE+GLYCOL~~'>~~PG4~~</scene>, <scene name='pdbligand=~~PIS~~:~~TRIHYDROGEN~~+~~THIODIPHOSPHATE~~'>~~PIS~~</scene>, <scene name='pdbligand=~~SO4~~:~~SULFATE~~+~~ION~~'>~~SO4~~</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PIS:TRIHYDROGEN+THIODIPHOSPHATE'>PIS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~MLY~~:~~N-DIMETHYL-LYSINE~~'>~~MLY~~</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ee7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee7 OCA], [https://pdbe.org/4ee7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ee7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ee7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ee7 ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ee8\|4ee8]], [[4ee6\|4ee6]]</td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">epzP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1900 AS 4.1619])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ee7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee7 OCA], [~~http~~://pdbe.org/4ee7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ee7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ee7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ee7 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/E5KWG9_STRCM E5KWG9_STRCM]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~As 4 1619~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Stehle, T~~]]	+	[[Category: Streptomyces cinnamonensis]]
-	[[Category: ~~Zocher, G~~]]	+	[[Category: Stehle T]]
-	[[Category: ~~Dihydrophenazine carboxylate prenyltransferase]]~~	+	[[Category: Zocher G]]
-	~~[[Category: Pt fold]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 02:52, 11 December 2016

2016-12-11T02:52:42Z

←Older revision		Revision as of 02:52, 11 December 2016
Line 1:		Line 1:
		+
	==Crystal Structure of the Novel Phenazine Prenyltransferase EpzP in complex with S-thiolodiphosphate (methylated)==		==Crystal Structure of the Novel Phenazine Prenyltransferase EpzP in complex with S-thiolodiphosphate (methylated)==
	<StructureSection load='4ee7' size='340' side='right' caption='[[4ee7]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='4ee7' size='340' side='right' caption='[[4ee7]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ee7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_cinnamonensis Streptomyces cinnamonensis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EE7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ee7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1619 As 4.1619]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EE7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PIS:TRIHYDROGEN+THIODIPHOSPHATE'>PIS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PIS:TRIHYDROGEN+THIODIPHOSPHATE'>PIS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ee8\|4ee8]], [[4ee6\|4ee6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ee8\|4ee8]], [[4ee6\|4ee6]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">epzP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1900 ~~Streptomyces cinnamonensis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">epzP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1900 AS 4.1619])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ee7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ee7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ee7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ee7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee7 OCA], [http://pdbe.org/4ee7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ee7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ee7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ee7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ee7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces cinnamonensis~~]]	+	[[Category: As 4 1619]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]
	[[Category: Zocher, G]]		[[Category: Zocher, G]]

OCA at 16:59, 9 December 2014

2014-12-09T16:59:34Z

←Older revision		Revision as of 16:59, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4ee7.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of the Novel Phenazine Prenyltransferase EpzP in complex with S-thiolodiphosphate (methylated)==
		+	<StructureSection load='4ee7' size='340' side='right' caption='[[4ee7]], [[Resolution\|resolution]] 1.67Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ee7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cinnamonensis Streptomyces cinnamonensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EE7 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PIS:TRIHYDROGEN+THIODIPHOSPHATE'>PIS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ee8\|4ee8]], [[4ee6\|4ee6]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">epzP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1900 Streptomyces cinnamonensis])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ee7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ee7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ee7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ee7 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Prenyltransferases (PTs) catalyze the regioselective transfer of prenyl moieties onto aromatic substrates in biosynthetic pathways of microbial secondary metabolites. Therefore, these enzymes contribute to the chemical diversity of natural products. Prenylation is frequently essential for the pharmacological properties of these metabolites, including their antibiotic and antitumor activities. Recently, the first phenazine PTs, termed EpzP and PpzP, were isolated and biochemically characterized. The two enzymes play a central role in the biosynthesis of endophenazines by catalyzing the regiospecific prenylation of 5,10-dihydrophenazine-1-carboxylic acid (dhPCA) in the secondary metabolism of two different Streptomyces strains. Here we report crystal structures of EpzP in its unliganded state as well as bound to S-thiolodiphosphate (SPP), thus defining the first three-dimensional structures for any phenazine PT. A model of a ternary complex resulted from in silico modeling of dhPCA and site-directed mutagenesis. The structural analysis provides detailed insight into the likely mechanism of phenazine prenylation. The catalytic mechanism suggested by the structure identifies amino acids that are required for catalysis. Inspection of the structures and the model of the ternary complex furthermore allowed us to rationally engineer EpzP variants with up to 14-fold higher catalytic reaction rate compared to the wild-type enzyme. This study therefore provides a solid foundation for additional enzyme modifications that should result in efficient, tailor-made biocatalysts for phenazines production.

-	~~{{STRUCTURE_4ee7\| PDB=4ee7 \| SCENE= }}~~	+	Structure-based engineering increased the catalytic turnover rate of a novel phenazine prenyltransferase.,Zocher G, Saleh O, Heim JB, Herbst DA, Heide L, Stehle T PLoS One. 2012;7(10):e48427. doi: 10.1371/journal.pone.0048427. Epub 2012 Oct 31. PMID:23119011<ref>PMID:23119011</ref>

-	~~===Crystal Structure~~ of the ~~Novel Phenazine Prenyltransferase EpzP in complex with~~ S~~-thiolodiphosphate (methylated)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	==~~About this Structure~~==	+	<references/>
-	~~[[4ee7]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Streptomyces_cinnamonensis Streptomyces cinnamonensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE7 OCA].~~	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces cinnamonensis]]		[[Category: Streptomyces cinnamonensis]]
-	[[Category: Stehle, T.]]	+	[[Category: Stehle, T]]
-	[[Category: Zocher, G.]]	+	[[Category: Zocher, G]]
	[[Category: Dihydrophenazine carboxylate prenyltransferase]]		[[Category: Dihydrophenazine carboxylate prenyltransferase]]
	[[Category: Pt fold]]		[[Category: Pt fold]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 06:44, 14 November 2012

2012-11-14T06:44:32Z

←Older revision		Revision as of 06:44, 14 November 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4ee7.jpg\|left\|200px]]

-	~~The entry~~ 4ee7 ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4ee7\| PDB=4ee7 \| SCENE= }}

-	~~Authors: Zocher, G., Stehle, T.~~	+	===Crystal Structure of the Novel Phenazine Prenyltransferase EpzP in complex with S-thiolodiphosphate (methylated)===

-	~~Description~~:	+
		+	==About this Structure==
		+	[[4ee7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cinnamonensis Streptomyces cinnamonensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EE7 OCA].
		+	[[Category: Streptomyces cinnamonensis]]
		+	[[Category: Stehle, T.]]
		+	[[Category: Zocher, G.]]
		+	[[Category: Dihydrophenazine carboxylate prenyltransferase]]
		+	[[Category: Pt fold]]
		+	[[Category: Transferase]]

OCA at 05:25, 30 May 2012

2012-05-30T05:25:37Z

←Older revision		Revision as of 05:25, 30 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ee7 is ON HOLD	+	The entry 4ee7 is ON HOLD until Paper Publication

	Authors: Zocher, G., Stehle, T.		Authors: Zocher, G., Stehle, T.

	Description:		Description:

OCA at 07:30, 18 April 2012

2012-04-18T07:30:44Z

←Older revision		Revision as of 07:30, 18 April 2012
Line 5:		Line 5:
	Authors: Zocher, G., Stehle, T.		Authors: Zocher, G., Stehle, T.

-	Description: ~~Crystal Structure of~~	+	Description:

OCA: Protected "4ee7" [edit=sysop:move=sysop]

2012-04-04T05:50:09Z

Protected "4ee7" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:50, 4 April 2012

OCA: New page: '''Unreleased structure''' The entry 4ee7 is ON HOLD Authors: Zocher, G., Stehle, T. Description: Crystal Structure of

2012-04-04T05:50:08Z

New page: '''Unreleased structure''' The entry 4ee7 is ON HOLD Authors: Zocher, G., Stehle, T. Description: Crystal Structure of

New page

'''Unreleased structure'''

The entry 4ee7 is ON HOLD

Authors: Zocher, G., Stehle, T.

Description: Crystal Structure of