4ehq - Revision history

OCA at 15:01, 14 March 2024

2024-03-14T15:01:43Z

←Older revision		Revision as of 15:01, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ehq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EHQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ehq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EHQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GBL:GAMMA-BUTYROLACTONE'>GBL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9005Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GBL:GAMMA-BUTYROLACTONE'>GBL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ehq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehq OCA], [https://pdbe.org/4ehq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ehq RCSB], [https://www.ebi.ac.uk/pdbsum/4ehq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ehq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ehq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehq OCA], [https://pdbe.org/4ehq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ehq RCSB], [https://www.ebi.ac.uk/pdbsum/4ehq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ehq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CALM1_RAT CALM1_RAT]] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Mediates calcium-dependent inactivation of CACNA1C. Positively regulates calcium-activated potassium channel activity of KCNN2.[UniProtKB:P62158]	+	[https://www.uniprot.org/uniprot/CALM1_RAT CALM1_RAT] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Mediates calcium-dependent inactivation of CACNA1C. Positively regulates calcium-activated potassium channel activity of KCNN2.[UniProtKB:P62158]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Orai1 is a plasma membrane protein which in its tetrameric form is responsible for calcium influx from the extracellular environment into cytosol in response to interaction with the Ca2+-depletion sensor STIM1. This is followed by a fast Ca2+/calmodulin (CaM)-dependent inhibition, resulting from CaM binding to an Orai1 region called calmodulin binding domain (CMBD). The interaction between Orai1 and CaM at the atomic level remains unknown. Here, we report the crystal structure of a CaM/Orai1-CMBD complex showing one CMBD bound to the C-terminal lobe of CaM, differing from other CaM/target protein complexes, in which both N- and C-terminal lobes of CaM (CaM-N and CaM-C) are involved in target binding. Orai1-CMBD binds CaM-C mainly through hydrophobic interactions, involving primarily residue W76 of Orai1-CMBD which interacts with the hydrophobic pocket of CaM-C. However, NMR data, isothermal titration calorimetry data, and pull-down assays indicated that CaM-N and CaM-C both can bind Orai1-CMBD, with CaM-N having ~4 times weaker affinity than CaM-C. Pull-down assays of a Orai1-CMBD(W76E) mutant, gel filtration chromatography data and NOE signals indicated that CaM-N and CaM-C can each bind one Orai1-CMBD. Thus our studies support an unusual, extended 1:2 binding mode of CaM to Orai1-CMBDs, and quantify the affinity of Orai1 for CaM. We propose a two-step mechanism for CaM-dependent Orai1 inactivation initiated by binding of the C-lobe of CaM to the CMBD of one Orai1 followed by the binding of the N-lobe of CaM to the CMBD of a neighboring Orai1.	+
-		+
-	Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode.,Liu Y, Zheng X, Mueller GA, Sobhany M, Derose EF, Zhang Y, London RE, Birnbaumer L J Biol Chem. 2012 Oct 29. PMID:23109337<ref>PMID:23109337</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4ehq" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Calmodulin 3D structures\|Calmodulin 3D structures]]		*[[Calmodulin 3D structures\|Calmodulin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:11, 28 September 2022

2022-09-28T07:11:53Z

←Older revision		Revision as of 07:11, 28 September 2022
Line 1:		Line 1:

	==Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode==		==Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode==
-	<StructureSection load='4ehq' size='340' side='right' caption='[[4ehq]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='4ehq' size='340' side='right'caption='[[4ehq]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ehq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EHQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ehq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EHQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GBL:GAMMA-BUTYROLACTONE'>GBL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GBL:GAMMA-BUTYROLACTONE'>GBL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Calm1, Calm, Cam, Cam1, Calm2, Cam2, Camb, Calm3, Cam3, Camc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ehq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehq OCA], [https://pdbe.org/4ehq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ehq RCSB], [https://www.ebi.ac.uk/pdbsum/4ehq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ehq ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ehq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehq OCA], [~~http~~://pdbe.org/4ehq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ehq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ehq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ehq ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Disease ==
-	[[http://www.uniprot.org/uniprot/CRCM1_HUMAN CRCM1_HUMAN]] Combined immunodeficiency due to ORAI1 deficiency. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/~~CRCM1_HUMAN CRCM1_HUMAN~~]] ~~Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which~~ mediates ~~Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by~~ the ~~Ca(2+) sensor~~, ~~STIM1. CRAC~~ channels ~~are the main pathway for Ca(2+) influx in T-cells~~ and ~~promote~~ the ~~immune response~~ to ~~pathogens~~ by ~~activating~~ the ~~transcription factor NFAT~~.~~<ref>PMID~~:~~16766533</ref> <ref>PMID:16807233</ref> <ref>PMID:16733527</ref> <ref>PMID:16645049</ref> <ref>PMID:16582901</ref>~~	+	[[https://www.uniprot.org/uniprot/CALM1_RAT CALM1_RAT]] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Mediates calcium-dependent inactivation of CACNA1C. Positively regulates calcium-activated potassium channel activity of KCNN2.[UniProtKB:P62158]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 20:

	==See Also==		==See Also==
-	*[[Calmodulin\|Calmodulin]]	+	*[[Calmodulin 3D structures\|Calmodulin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Buffalo rat~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Birnbaumer, L~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~DeRose, E F~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: ~~Liu, Y~~]]	+	[[Category: Birnbaumer L]]
-	[[Category: ~~London, R E~~]]	+	[[Category: DeRose EF]]
-	[[Category: ~~Mueller, G A]]~~	+	[[Category: Liu Y]]
-	~~[[Category: Sobhany, M]]~~	+	[[Category: London RE]]
-	~~[[Category: Zhang,~~ Y]]	+	[[Category: Mueller GA]]
-	[[Category: ~~Zheng, X~~]]	+	[[Category: Sobhany M]]
-	[[Category: ~~Calcium binding~~]]	+	[[Category: Zhang Y]]
-	[[Category: ~~Calcium dependent inactivation~~]]	+	[[Category: Zheng X]]
-	[[Category: ~~Calcium-dependent inactivation~~]]	+
-	[[Category: ~~Calmodulin]]~~	+
-	~~[[Category: Calmodulin binding domain of orai1]]~~	+
-	~~[[Category: Cytosol]]~~	+
-	~~[[Category: Ef hand]]~~	+
-	~~[[Category: None]]~~	+
-	~~[[Category: Orai1]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 02:49, 11 December 2016

2016-12-11T02:49:32Z

←Older revision		Revision as of 02:49, 11 December 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode==		==Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode==
	<StructureSection load='4ehq' size='340' side='right' caption='[[4ehq]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4ehq' size='340' side='right' caption='[[4ehq]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ehq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rattus_norvegicus Rattus norvegicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EHQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ehq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EHQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GBL:GAMMA-BUTYROLACTONE'>GBL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GBL:GAMMA-BUTYROLACTONE'>GBL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Calm1, Calm, Cam, Cam1, Calm2, Cam2, Camb, Calm3, Cam3, Camc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 ~~Rattus norvegicus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Calm1, Calm, Cam, Cam1, Calm2, Cam2, Camb, Calm3, Cam3, Camc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ehq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ehq RCSB], [http://www.ebi.ac.uk/pdbsum/4ehq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ehq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehq OCA], [http://pdbe.org/4ehq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ehq RCSB], [http://www.ebi.ac.uk/pdbsum/4ehq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ehq ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ehq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Rattus norvegicus~~]]	+	[[Category: Buffalo rat]]
	[[Category: Birnbaumer, L]]		[[Category: Birnbaumer, L]]
	[[Category: DeRose, E F]]		[[Category: DeRose, E F]]

OCA at 16:03, 9 December 2014

2014-12-09T16:03:25Z

←Older revision		Revision as of 16:03, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4ehq.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode==
		+	<StructureSection load='4ehq' size='340' side='right' caption='[[4ehq]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ehq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EHQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GBL:GAMMA-BUTYROLACTONE'>GBL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Calm1, Calm, Cam, Cam1, Calm2, Cam2, Camb, Calm3, Cam3, Camc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ehq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ehq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ehq RCSB], [http://www.ebi.ac.uk/pdbsum/4ehq PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/CRCM1_HUMAN CRCM1_HUMAN]] Combined immunodeficiency due to ORAI1 deficiency. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CRCM1_HUMAN CRCM1_HUMAN]] Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.<ref>PMID:16766533</ref> <ref>PMID:16807233</ref> <ref>PMID:16733527</ref> <ref>PMID:16645049</ref> <ref>PMID:16582901</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Orai1 is a plasma membrane protein which in its tetrameric form is responsible for calcium influx from the extracellular environment into cytosol in response to interaction with the Ca2+-depletion sensor STIM1. This is followed by a fast Ca2+/calmodulin (CaM)-dependent inhibition, resulting from CaM binding to an Orai1 region called calmodulin binding domain (CMBD). The interaction between Orai1 and CaM at the atomic level remains unknown. Here, we report the crystal structure of a CaM/Orai1-CMBD complex showing one CMBD bound to the C-terminal lobe of CaM, differing from other CaM/target protein complexes, in which both N- and C-terminal lobes of CaM (CaM-N and CaM-C) are involved in target binding. Orai1-CMBD binds CaM-C mainly through hydrophobic interactions, involving primarily residue W76 of Orai1-CMBD which interacts with the hydrophobic pocket of CaM-C. However, NMR data, isothermal titration calorimetry data, and pull-down assays indicated that CaM-N and CaM-C both can bind Orai1-CMBD, with CaM-N having ~4 times weaker affinity than CaM-C. Pull-down assays of a Orai1-CMBD(W76E) mutant, gel filtration chromatography data and NOE signals indicated that CaM-N and CaM-C can each bind one Orai1-CMBD. Thus our studies support an unusual, extended 1:2 binding mode of CaM to Orai1-CMBDs, and quantify the affinity of Orai1 for CaM. We propose a two-step mechanism for CaM-dependent Orai1 inactivation initiated by binding of the C-lobe of CaM to the CMBD of one Orai1 followed by the binding of the N-lobe of CaM to the CMBD of a neighboring Orai1.

-	~~{{STRUCTURE_4ehq\| PDB=4ehq \| SCENE= }}~~	+	Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode.,Liu Y, Zheng X, Mueller GA, Sobhany M, Derose EF, Zhang Y, London RE, Birnbaumer L J Biol Chem. 2012 Oct 29. PMID:23109337<ref>PMID:23109337</ref>

-	~~===Crystal Structure~~ of ~~Calmodulin Binding Domain~~ of ~~Orai1 in Complex with Ca2+~~/~~Calmodulin Displays a Unique Binding Mode===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_23109337}}~~	+	==See Also==
-		+	*[[Calmodulin\|Calmodulin]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4ehq~~]] ~~is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHQ OCA].~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
-	[[Category: Birnbaumer, L.]]	+	[[Category: Birnbaumer, L]]
-	[[Category: DeRose, E F.]]	+	[[Category: DeRose, E F]]
-	[[Category: Liu, Y.]]	+	[[Category: Liu, Y]]
-	[[Category: London, R E.]]	+	[[Category: London, R E]]
-	[[Category: Mueller, G A.]]	+	[[Category: Mueller, G A]]
-	[[Category: Sobhany, M.]]	+	[[Category: Sobhany, M]]
-	[[Category: Zhang, Y.]]	+	[[Category: Zhang, Y]]
-	[[Category: Zheng, X.]]	+	[[Category: Zheng, X]]
	[[Category: Calcium binding]]		[[Category: Calcium binding]]
	[[Category: Calcium dependent inactivation]]		[[Category: Calcium dependent inactivation]]

OCA at 07:59, 2 January 2013

2013-01-02T07:59:17Z

←Older revision		Revision as of 07:59, 2 January 2013
Line 5:		Line 5:
	===Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode===		===Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode===

		+	{{ABSTRACT_PUBMED_23109337}}

	==About this Structure==		==About this Structure==

OCA at 06:38, 14 November 2012

2012-11-14T06:38:06Z

←Older revision		Revision as of 06:38, 14 November 2012
Line 1:		Line 1:
-	[[Image:4ehq.~~jpg~~\|left\|200px]]	+	[[Image:4ehq.png\|left\|200px]]

	{{STRUCTURE_4ehq\| PDB=4ehq \| SCENE= }}		{{STRUCTURE_4ehq\| PDB=4ehq \| SCENE= }}

OCA at 14:01, 8 November 2012

2012-11-08T14:01:44Z

←Older revision		Revision as of 14:01, 8 November 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:4ehq.jpg\|left\|200px]]

-	~~The entry~~ 4ehq ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4ehq\| PDB=4ehq \| SCENE= }}

-	~~Authors: Liu, Y., Zheng, X., Mueller, G.A., Sobhany, M., DeRose, E.F., Zhang, Y., London, R.E., Birnbaumer, L.~~	+	===Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode===

-	~~Description: Crystal~~ Structure ~~of Calmodulin Binding Domain of Orai1 in Complex~~ with ~~Ca2+~~/Calmodulin ~~Displays a Unique Binding Mode~~	+
		+	==About this Structure==
		+	[[4ehq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EHQ OCA].
		+	[[Category: Rattus norvegicus]]
		+	[[Category: Birnbaumer, L.]]
		+	[[Category: DeRose, E F.]]
		+	[[Category: Liu, Y.]]
		+	[[Category: London, R E.]]
		+	[[Category: Mueller, G A.]]
		+	[[Category: Sobhany, M.]]
		+	[[Category: Zhang, Y.]]
		+	[[Category: Zheng, X.]]
		+	[[Category: Calcium binding]]
		+	[[Category: Calcium dependent inactivation]]
		+	[[Category: Calcium-dependent inactivation]]
		+	[[Category: Calmodulin]]
		+	[[Category: Calmodulin binding domain of orai1]]
		+	[[Category: Cytosol]]
		+	[[Category: Ef hand]]
		+	[[Category: None]]
		+	[[Category: Orai1]]
		+	[[Category: Protein binding]]

OCA at 05:37, 23 May 2012

2012-05-23T05:37:06Z

←Older revision		Revision as of 05:37, 23 May 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ehq is ON HOLD	+	The entry 4ehq is ON HOLD until Paper Publication

-	Authors: Liu, Y., Zheng, X., Mueller, G.A., Sobhany, H, DeRose, E.F., Zhang, Y., London, R.E., Birnbaumer, L.	+	Authors: Liu, Y., Zheng, X., Mueller, G.A., Sobhany, M., DeRose, E.F., Zhang, Y., London, R.E., Birnbaumer, L.

	Description: Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode		Description: Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode

OCA: Protected "4ehq" [edit=sysop:move=sysop]

2012-04-11T06:43:34Z

Protected "4ehq" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:43, 11 April 2012

OCA: New page: '''Unreleased structure''' The entry 4ehq is ON HOLD Authors: Liu, Y., Zheng, X., Mueller, G.A., Sobhany, H, DeRose, E.F., Zhang, Y., London, R.E., Birnbaumer, L. Description: Crystal ...

2012-04-11T06:43:33Z

New page: '''Unreleased structure''' The entry 4ehq is ON HOLD Authors: Liu, Y., Zheng, X., Mueller, G.A., Sobhany, H, DeRose, E.F., Zhang, Y., London, R.E., Birnbaumer, L. Description: Crystal ...

New page

'''Unreleased structure'''

The entry 4ehq is ON HOLD

Authors: Liu, Y., Zheng, X., Mueller, G.A., Sobhany, H, DeRose, E.F., Zhang, Y., London, R.E., Birnbaumer, L.

Description: Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode