4eql - Revision history

OCA at 15:07, 14 March 2024

2024-03-14T15:07:32Z

←Older revision		Revision as of 15:07, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eql]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EQL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eql]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EQL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eql OCA], [https://pdbe.org/4eql PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eql RCSB], [https://www.ebi.ac.uk/pdbsum/4eql PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eql ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eql OCA], [https://pdbe.org/4eql PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eql RCSB], [https://www.ebi.ac.uk/pdbsum/4eql PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eql ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GH312_ARATH GH312_ARATH] Catalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR).<ref>PMID:18266921</ref> <ref>PMID:10224270</ref> <ref>PMID:11846877</ref> <ref>PMID:16353557</ref> <ref>PMID:17918621</ref> <ref>PMID:17521413</ref> <ref>PMID:17468220</ref> <ref>PMID:19189963</ref>		[https://www.uniprot.org/uniprot/GH312_ARATH GH312_ARATH] Catalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR).<ref>PMID:18266921</ref> <ref>PMID:10224270</ref> <ref>PMID:11846877</ref> <ref>PMID:16353557</ref> <ref>PMID:17918621</ref> <ref>PMID:17521413</ref> <ref>PMID:17468220</ref> <ref>PMID:19189963</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Acyl acid amido synthetases of the GH3 family act as critical prereceptor modulators of plant hormone action; however, the molecular basis for their hormone selectivity is unclear. Here, we report the crystal structures of benzoate-specific Arabidopsis thaliana AtGH3.12/PBS3 and jasmonic acid (JA)-specific AtGH3.11/JAR1. These structures, combined with biochemical analysis, define features for the conjugation of amino acids to diverse acyl acid substrates and highlight the importance of conformational changes in the C-terminal domain for catalysis. We also identify residues forming the acyl acid binding site across the GH3 family and residues critical for amino acid recognition. Our results demonstrate how a highly adaptable three-dimensional scaffold is used for the evolution of promiscuous activity across an enzyme family for modulation of plant signaling molecules.
-
-	Structural Basis for Prereceptor Modulation of Plant Hormones by GH3 Proteins.,Westfall CS, Zubieta C, Herrmann J, Kapp U, Nanao MH, Jez JM Science. 2012 May 24. PMID:22628555<ref>PMID:22628555</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4eql" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:09, 7 October 2022

2022-10-07T04:09:24Z

←Older revision		Revision as of 04:09, 7 October 2022
Line 1:		Line 1:

	==Crystal Structure of GH3.12 in complex with AMP and salicylate==		==Crystal Structure of GH3.12 in complex with AMP and salicylate==
-	<StructureSection load='4eql' size='340' side='right' caption='[[4eql]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='4eql' size='340' side='right'caption='[[4eql]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4eql]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EQL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4eql]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EQL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eq4\|4eq4]], [[4epl\|4epl]], [[4epm\|4epm]], [[4ewv\|4ewv]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eql OCA], [https://pdbe.org/4eql PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eql RCSB], [https://www.ebi.ac.uk/pdbsum/4eql PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eql ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GH3.12, GDG1, PBS3, WIN3, At5g13320, T22N19.5, T31B5.140 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4eql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eql OCA], [~~http~~://pdbe.org/4eql PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4eql RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4eql PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4eql ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GH312_ARATH GH312_ARATH]] Catalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR).<ref>PMID:18266921</ref> <ref>PMID:10224270</ref> <ref>PMID:11846877</ref> <ref>PMID:16353557</ref> <ref>PMID:17918621</ref> <ref>PMID:17521413</ref> <ref>PMID:17468220</ref> <ref>PMID:19189963</ref>	+	[https://www.uniprot.org/uniprot/GH312_ARATH GH312_ARATH] Catalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR).<ref>PMID:18266921</ref> <ref>PMID:10224270</ref> <ref>PMID:11846877</ref> <ref>PMID:16353557</ref> <ref>PMID:17918621</ref> <ref>PMID:17521413</ref> <ref>PMID:17468220</ref> <ref>PMID:19189963</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
-	[[Category: Herrmann, J]]	+	[[Category: Large Structures]]
-	[[Category: Jez, J]]	+	[[Category: Herrmann J]]
-	[[Category: Nanao, M]]	+	[[Category: Jez J]]
-	[[Category: Westfall, C]]	+	[[Category: Nanao M]]
-	[[Category: Zubieta, C]]	+	[[Category: Westfall C]]
-	~~[[Category: Acyl adenylase]]~~	+	[[Category: Zubieta C]]
-	~~[[Category: Amino acid conjugation]]~~	+
-	~~[[Category: Firefly luciferase family]]~~	+
-	~~[[Category: Ligase~~]]	+

OCA at 00:35, 5 August 2016

2016-08-05T00:35:22Z

←Older revision		Revision as of 00:35, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of GH3.12 in complex with AMP and salicylate==		==Crystal Structure of GH3.12 in complex with AMP and salicylate==
	<StructureSection load='4eql' size='340' side='right' caption='[[4eql]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4eql' size='340' side='right' caption='[[4eql]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4eql]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arabidopsis_thaliana Arabidopsis thaliana~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EQL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4eql]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EQL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eq4\|4eq4]], [[4epl\|4epl]], [[4epm\|4epm]], [[4ewv\|4ewv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eq4\|4eq4]], [[4epl\|4epl]], [[4epm\|4epm]], [[4ewv\|4ewv]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GH3.12, GDG1, PBS3, WIN3, At5g13320, T22N19.5, T31B5.140 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ~~Arabidopsis thaliana~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GH3.12, GDG1, PBS3, WIN3, At5g13320, T22N19.5, T31B5.140 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eql OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eql RCSB], [http://www.ebi.ac.uk/pdbsum/4eql PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eql OCA], [http://pdbe.org/4eql PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eql RCSB], [http://www.ebi.ac.uk/pdbsum/4eql PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eql ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4eql" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arabidopsis thaliana~~]]	+	[[Category: Arath]]
	[[Category: Herrmann, J]]		[[Category: Herrmann, J]]
	[[Category: Jez, J]]		[[Category: Jez, J]]

OCA at 21:14, 24 December 2014

2014-12-24T21:14:03Z

←Older revision		Revision as of 21:14, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eql]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EQL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eql]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EQL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eq4\|4eq4]], [[4epl\|4epl]], [[4epm\|4epm]], [[4ewv\|4ewv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eq4\|4eq4]], [[4epl\|4epl]], [[4epm\|4epm]], [[4ewv\|4ewv]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GH3.12, GDG1, PBS3, WIN3, At5g13320, T22N19.5, T31B5.140 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GH3.12, GDG1, PBS3, WIN3, At5g13320, T22N19.5, T31B5.140 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eql OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eql RCSB], [http://www.ebi.ac.uk/pdbsum/4eql PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eql OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eql RCSB], [http://www.ebi.ac.uk/pdbsum/4eql PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GH312_ARATH GH312_ARATH]] Catalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR).<ref>PMID:18266921</ref> <ref>PMID:10224270</ref> <ref>PMID:11846877</ref> <ref>PMID:16353557</ref> <ref>PMID:17918621</ref> <ref>PMID:17521413</ref> <ref>PMID:17468220</ref> <ref>PMID:19189963</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
-	[[Category: Herrmann, J.]]	+	[[Category: Herrmann, J]]
-	[[Category: Jez, J.]]	+	[[Category: Jez, J]]
-	[[Category: Nanao, M.]]	+	[[Category: Nanao, M]]
-	[[Category: Westfall, C.]]	+	[[Category: Westfall, C]]
-	[[Category: Zubieta, C.]]	+	[[Category: Zubieta, C]]
	[[Category: Acyl adenylase]]		[[Category: Acyl adenylase]]
	[[Category: Amino acid conjugation]]		[[Category: Amino acid conjugation]]
	[[Category: Firefly luciferase family]]		[[Category: Firefly luciferase family]]
	[[Category: Ligase]]		[[Category: Ligase]]

OCA at 07:56, 5 June 2014

2014-06-05T07:56:52Z

←Older revision		Revision as of 07:56, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4eql.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of GH3.12 in complex with AMP and salicylate==
		+	<StructureSection load='4eql' size='340' side='right' caption='[[4eql]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4eql]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EQL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eq4\|4eq4]], [[4epl\|4epl]], [[4epm\|4epm]], [[4ewv\|4ewv]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GH3.12, GDG1, PBS3, WIN3, At5g13320, T22N19.5, T31B5.140 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eql OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eql RCSB], [http://www.ebi.ac.uk/pdbsum/4eql PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acyl acid amido synthetases of the GH3 family act as critical prereceptor modulators of plant hormone action; however, the molecular basis for their hormone selectivity is unclear. Here, we report the crystal structures of benzoate-specific Arabidopsis thaliana AtGH3.12/PBS3 and jasmonic acid (JA)-specific AtGH3.11/JAR1. These structures, combined with biochemical analysis, define features for the conjugation of amino acids to diverse acyl acid substrates and highlight the importance of conformational changes in the C-terminal domain for catalysis. We also identify residues forming the acyl acid binding site across the GH3 family and residues critical for amino acid recognition. Our results demonstrate how a highly adaptable three-dimensional scaffold is used for the evolution of promiscuous activity across an enzyme family for modulation of plant signaling molecules.

-	~~{{STRUCTURE_4eql\| PDB=4eql \| SCENE= }}~~	+	Structural Basis for Prereceptor Modulation of Plant Hormones by GH3 Proteins.,Westfall CS, Zubieta C, Herrmann J, Kapp U, Nanao MH, Jez JM Science. 2012 May 24. PMID:22628555<ref>PMID:22628555</ref>

-	~~===Crystal Structure~~ of ~~GH3~~.~~12 in complex with AMP and salicylate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22628555}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[4eql]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQL OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022628555<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Herrmann, J.]]		[[Category: Herrmann, J.]]

OCA at 06:04, 25 July 2012

2012-07-25T06:04:01Z

←Older revision		Revision as of 06:04, 25 July 2012
Line 1:		Line 1:
-	[[Image:4eql.~~jpg~~\|left\|200px]]	+	[[Image:4eql.png\|left\|200px]]

	{{STRUCTURE_4eql\| PDB=4eql \| SCENE= }}		{{STRUCTURE_4eql\| PDB=4eql \| SCENE= }}

OCA at 07:44, 20 June 2012

2012-06-20T07:44:09Z

←Older revision		Revision as of 07:44, 20 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4eql.jpg\|left\|200px]]

-	~~The entry~~ 4eql ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4eql\| PDB=4eql \| SCENE= }}

-	~~Authors: Westfall, C., Zubieta, C., Nanao, M., Herrmann, J., Jez, J~~.	+	===Crystal Structure of GH3.12 in complex with AMP and salicylate===

-	~~Description: Crystal~~ Structure ~~of GH3.12 in complex~~ with ~~AMP and salicylate~~	+	{{ABSTRACT_PUBMED_22628555}}
		+
		+	==About this Structure==
		+	[[4eql]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQL OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022628555</ref><references group="xtra"/>
		+	[[Category: Arabidopsis thaliana]]
		+	[[Category: Herrmann, J.]]
		+	[[Category: Jez, J.]]
		+	[[Category: Nanao, M.]]
		+	[[Category: Westfall, C.]]
		+	[[Category: Zubieta, C.]]
		+	[[Category: Acyl adenylase]]
		+	[[Category: Amino acid conjugation]]
		+	[[Category: Firefly luciferase family]]
		+	[[Category: Ligase]]

OCA at 07:16, 6 June 2012

2012-06-06T07:16:42Z

←Older revision		Revision as of 07:16, 6 June 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4eql is ON HOLD	+	The entry 4eql is ON HOLD until Paper Publication

	Authors: Westfall, C., Zubieta, C., Nanao, M., Herrmann, J., Jez, J.		Authors: Westfall, C., Zubieta, C., Nanao, M., Herrmann, J., Jez, J.

	Description: Crystal Structure of GH3.12 in complex with AMP and salicylate		Description: Crystal Structure of GH3.12 in complex with AMP and salicylate

OCA: Protected "4eql" [edit=sysop:move=sysop]

2012-04-25T08:57:31Z

Protected "4eql" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:57, 25 April 2012

OCA: New page: '''Unreleased structure''' The entry 4eql is ON HOLD Authors: Westfall, C., Zubieta, C., Nanao, M., Herrmann, J., Jez, J. Description: Crystal Structure of GH3.12 in complex with AMP a...

2012-04-25T08:57:31Z

New page: '''Unreleased structure''' The entry 4eql is ON HOLD Authors: Westfall, C., Zubieta, C., Nanao, M., Herrmann, J., Jez, J. Description: Crystal Structure of GH3.12 in complex with AMP a...

New page

'''Unreleased structure'''

The entry 4eql is ON HOLD

Authors: Westfall, C., Zubieta, C., Nanao, M., Herrmann, J., Jez, J.

Description: Crystal Structure of GH3.12 in complex with AMP and salicylate