4eri - Revision history

OCA at 15:07, 14 March 2024

2024-03-14T15:07:54Z

←Older revision		Revision as of 15:07, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eri]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ERI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ERI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eri]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ERI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ERI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.0006Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eri OCA], [https://pdbe.org/4eri PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eri RCSB], [https://www.ebi.ac.uk/pdbsum/4eri PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eri ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eri OCA], [https://pdbe.org/4eri PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eri RCSB], [https://www.ebi.ac.uk/pdbsum/4eri PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eri ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q83V25_PSEFL Q83V25_PSEFL]		[https://www.uniprot.org/uniprot/Q83V25_PSEFL Q83V25_PSEFL]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The previously reported crystal structures of alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (ACMSD) show a five-coordinate Zn(II)(His)(3)(Asp)(OH(2)) active site. The water ligand is H-bonded to a conserved His228 residue adjacent to the metal center in ACMSD from Pseudomonas fluorescens (PfACMSD). Site-directed mutagenesis of His228 to tyrosine and glycine in this study results in a complete or significant loss of activity. Metal analysis shows that H228Y and H228G contain iron rather than zinc, indicating that this residue plays a role in the metal selectivity of the protein. As-isolated H228Y displays a blue color, which is not seen in wild-type ACMSD. Quinone staining and resonance Raman analyses indicate that the blue color originates from Fe(III)-tyrosinate ligand-to-metal charge transfer. Co(II)-substituted H228Y ACMSD is brown in color and exhibits an electron paramagnetic resonance spectrum showing a high-spin Co(II) center with a well-resolved (59)Co (I = (7)/(2)) eight-line hyperfine splitting pattern. The X-ray crystal structures of as-isolated Fe-H228Y (2.8 A) and Co-substituted (2.4 A) and Zn-substituted H228Y (2.0 A resolution) support the spectroscopic assignment of metal ligation of the Tyr228 residue. The crystal structure of Zn-H228G (2.6 A) was also determined. These four structures show that the water ligand present in WT Zn-ACMSD is either missing (Fe-H228Y, Co-H228Y, and Zn-H228G) or disrupted (Zn-H228Y) in response to the His228 mutation. Together, these results highlight the importance of His228 for PfACMSD's metal specificity as well as maintaining a water molecule as a ligand of the metal center. His228 is thus proposed to play a role in activating the metal-bound water ligand for subsequent nucleophilic attack on the substrate.
-
-	Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-carboxymuconate-epsilon-semialdehyde Decarboxylase.,Huo L, Fielding AJ, Chen Y, Li T, Iwaki H, Hosler JP, Chen L, Hasegawa Y, Que L Jr, Liu A Biochemistry. 2012 Jul 24;51(29):5811-21. Epub 2012 Jul 12. PMID:22746257<ref>PMID:22746257</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4eri" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:10, 7 October 2022

2022-10-07T04:10:34Z

←Older revision		Revision as of 04:10, 7 October 2022
Line 1:		Line 1:

	==Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase==		==Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase==
-	<StructureSection load='4eri' size='340' side='right' caption='[[4eri]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4eri' size='340' side='right'caption='[[4eri]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4eri]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus~~ fluorescens ~~liquefaciens" flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ERI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ERI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4eri]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ERI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ERI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epk\|4epk]], [[4era\|4era]], [[4erg\|4erg]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eri OCA], [https://pdbe.org/4eri PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eri RCSB], [https://www.ebi.ac.uk/pdbsum/4eri PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eri ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nbaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 "Bacillus fluorescens liquefaciens" Flugge 1886])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4eri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eri OCA], [~~http~~://pdbe.org/4eri PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4eri RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4eri PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4eri ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q83V25_PSEFL Q83V25_PSEFL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ fluorescens ~~liquefaciens flugge 1886~~]]	+	[[Category: Large Structures]]
-	[[Category: Chen, L]]	+	[[Category: Pseudomonas fluorescens]]
-	[[Category: Chen, Y]]	+	[[Category: Chen L]]
-	[[Category: Fielding~~, A J~~]]	+	[[Category: Chen Y]]
-	[[Category: Hasegawa, Y]]	+	[[Category: Fielding AJ]]
-	[[Category: Hosler~~, J P~~]]	+	[[Category: Hasegawa Y]]
-	[[Category: Huo, L]]	+	[[Category: Hosler JP]]
-	[[Category: Iwaki, H]]	+	[[Category: Huo L]]
-	[[Category: Li, T]]	+	[[Category: Iwaki H]]
-	[[Category: Liu, A]]	+	[[Category: Li T]]
-	[[Category: Que, L]]	+	[[Category: Liu A]]
-	~~[[Category: Decarboxylase]]~~	+	[[Category: Que Jr L]]
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Tim barrel]]~~	+
-	~~[[Category: Zn~~]]	+

OCA at 15:38, 11 August 2016

2016-08-11T15:38:05Z

←Older revision		Revision as of 15:38, 11 August 2016
Line 1:		Line 1:
		+
	==Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase==		==Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase==
	<StructureSection load='4eri' size='340' side='right' caption='[[4eri]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4eri' size='340' side='right' caption='[[4eri]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4eri]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_fluorescens Pseudomonas~~ fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ERI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ERI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4eri]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus fluorescens liquefaciens" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ERI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ERI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epk\|4epk]], [[4era\|4era]], [[4erg\|4erg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epk\|4epk]], [[4era\|4era]], [[4erg\|4erg]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nbaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 ~~Pseudomonas~~ fluorescens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nbaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 "Bacillus fluorescens liquefaciens" Flugge 1886])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eri OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eri RCSB], [http://www.ebi.ac.uk/pdbsum/4eri PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eri OCA], [http://pdbe.org/4eri PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eri RCSB], [http://www.ebi.ac.uk/pdbsum/4eri PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eri ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4eri" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudomonas~~ fluorescens]]	+	[[Category: Bacillus fluorescens liquefaciens flugge 1886]]
	[[Category: Chen, L]]		[[Category: Chen, L]]
	[[Category: Chen, Y]]		[[Category: Chen, Y]]

OCA at 14:15, 4 January 2015

2015-01-04T14:15:31Z

←Older revision		Revision as of 14:15, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eri]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ERI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ERI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eri]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ERI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ERI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epk\|4epk]], [[4era\|4era]], [[4erg\|4erg]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epk\|4epk]], [[4era\|4era]], [[4erg\|4erg]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nbaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nbaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eri OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eri RCSB], [http://www.ebi.ac.uk/pdbsum/4eri PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eri OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eri RCSB], [http://www.ebi.ac.uk/pdbsum/4eri PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Pseudomonas fluorescens]]		[[Category: Pseudomonas fluorescens]]
-	[[Category: Chen, L.]]	+	[[Category: Chen, L]]
-	[[Category: Chen, Y.]]	+	[[Category: Chen, Y]]
-	[[Category: Fielding, A J.]]	+	[[Category: Fielding, A J]]
-	[[Category: Hasegawa, Y.]]	+	[[Category: Hasegawa, Y]]
-	[[Category: Hosler, J P.]]	+	[[Category: Hosler, J P]]
-	[[Category: Huo, L.]]	+	[[Category: Huo, L]]
-	[[Category: Iwaki, H.]]	+	[[Category: Iwaki, H]]
-	[[Category: Li, T.]]	+	[[Category: Li, T]]
-	[[Category: Liu, A.]]	+	[[Category: Liu, A]]
-	[[Category: Que, L.]]	+	[[Category: Que, L]]
	[[Category: Decarboxylase]]		[[Category: Decarboxylase]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 09:15, 11 June 2014

2014-06-11T09:15:52Z

←Older revision		Revision as of 09:15, 11 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4eri.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase==
		+	<StructureSection load='4eri' size='340' side='right' caption='[[4eri]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4eri]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ERI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ERI FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epk\|4epk]], [[4era\|4era]], [[4erg\|4erg]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nbaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eri OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eri RCSB], [http://www.ebi.ac.uk/pdbsum/4eri PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The previously reported crystal structures of alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (ACMSD) show a five-coordinate Zn(II)(His)(3)(Asp)(OH(2)) active site. The water ligand is H-bonded to a conserved His228 residue adjacent to the metal center in ACMSD from Pseudomonas fluorescens (PfACMSD). Site-directed mutagenesis of His228 to tyrosine and glycine in this study results in a complete or significant loss of activity. Metal analysis shows that H228Y and H228G contain iron rather than zinc, indicating that this residue plays a role in the metal selectivity of the protein. As-isolated H228Y displays a blue color, which is not seen in wild-type ACMSD. Quinone staining and resonance Raman analyses indicate that the blue color originates from Fe(III)-tyrosinate ligand-to-metal charge transfer. Co(II)-substituted H228Y ACMSD is brown in color and exhibits an electron paramagnetic resonance spectrum showing a high-spin Co(II) center with a well-resolved (59)Co (I = (7)/(2)) eight-line hyperfine splitting pattern. The X-ray crystal structures of as-isolated Fe-H228Y (2.8 A) and Co-substituted (2.4 A) and Zn-substituted H228Y (2.0 A resolution) support the spectroscopic assignment of metal ligation of the Tyr228 residue. The crystal structure of Zn-H228G (2.6 A) was also determined. These four structures show that the water ligand present in WT Zn-ACMSD is either missing (Fe-H228Y, Co-H228Y, and Zn-H228G) or disrupted (Zn-H228Y) in response to the His228 mutation. Together, these results highlight the importance of His228 for PfACMSD's metal specificity as well as maintaining a water molecule as a ligand of the metal center. His228 is thus proposed to play a role in activating the metal-bound water ligand for subsequent nucleophilic attack on the substrate.

-	~~{{STRUCTURE_4eri\| PDB=4eri \| SCENE= }}~~	+	Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-carboxymuconate-epsilon-semialdehyde Decarboxylase.,Huo L, Fielding AJ, Chen Y, Li T, Iwaki H, Hosler JP, Chen L, Hasegawa Y, Que L Jr, Liu A Biochemistry. 2012 Jul 24;51(29):5811-21. Epub 2012 Jul 12. PMID:22746257<ref>PMID:22746257</ref>

-	~~===Evidence for~~ a ~~Dual Role~~ of ~~an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22746257}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[4eri]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4ERI OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022746257<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Pseudomonas fluorescens]]		[[Category: Pseudomonas fluorescens]]
	[[Category: Chen, L.]]		[[Category: Chen, L.]]

OCA at 05:58, 22 August 2012

2012-08-22T05:58:20Z

←Older revision		Revision as of 05:58, 22 August 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4eri.jpg\|left\|200px]]

-	~~The entry~~ 4eri ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4eri\| PDB=4eri \| SCENE= }}

-	~~Authors: Huo, L., Fielding, A.J., Chen, Y., Li, T., Iwaki, H., Hosler, J.P., Chen, L., Hasegawa, Y., Que Jr., L., Liu, A.~~	+	===Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase===

-	~~Description: Evidence for~~ a ~~Dual Role of an Active Site Histidine in alpha~~-~~Amino-beta-Carboxymuconate-epsilon-Semialdehyde~~ Decarboxylase	+	{{ABSTRACT_PUBMED_22746257}}
		+
		+	==About this Structure==
		+	[[4eri]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ERI OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022746257</ref><references group="xtra"/>
		+	[[Category: Pseudomonas fluorescens]]
		+	[[Category: Chen, L.]]
		+	[[Category: Chen, Y.]]
		+	[[Category: Fielding, A J.]]
		+	[[Category: Hasegawa, Y.]]
		+	[[Category: Hosler, J P.]]
		+	[[Category: Huo, L.]]
		+	[[Category: Iwaki, H.]]
		+	[[Category: Li, T.]]
		+	[[Category: Liu, A.]]
		+	[[Category: Que, L.]]
		+	[[Category: Decarboxylase]]
		+	[[Category: Lyase]]
		+	[[Category: Metal-binding]]
		+	[[Category: Tim barrel]]
		+	[[Category: Zn]]

OCA at 05:28, 30 May 2012

2012-05-30T05:28:16Z

←Older revision		Revision as of 05:28, 30 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4eri is ON HOLD	+	The entry 4eri is ON HOLD until Paper Publication

	Authors: Huo, L., Fielding, A.J., Chen, Y., Li, T., Iwaki, H., Hosler, J.P., Chen, L., Hasegawa, Y., Que Jr., L., Liu, A.		Authors: Huo, L., Fielding, A.J., Chen, Y., Li, T., Iwaki, H., Hosler, J.P., Chen, L., Hasegawa, Y., Que Jr., L., Liu, A.

	Description: Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase		Description: Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase

OCA at 08:49, 9 May 2012

2012-05-09T08:49:21Z

←Older revision		Revision as of 08:49, 9 May 2012
Line 3:		Line 3:
	The entry 4eri is ON HOLD		The entry 4eri is ON HOLD

-	Authors: Huo, L., Fielding, A. J. , Chen, Y. , Li, T., Iwaki, H., Hosler, J.P., Chen, L., Hasegawa, Y., Que Jr., L., Liu, A.	+	Authors: Huo, L., Fielding, A.J., Chen, Y., Li, T., Iwaki, H., Hosler, J.P., Chen, L., Hasegawa, Y., Que Jr., L., Liu, A.

	Description: Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase		Description: Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase

OCA: Protected "4eri" [edit=sysop:move=sysop]

2012-05-02T18:57:13Z

Protected "4eri" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:57, 2 May 2012

OCA: New page: '''Unreleased structure''' The entry 4eri is ON HOLD Authors: Huo, L., Fielding, A. J. , Chen, Y. , Li, T., Iwaki, H., Hosler, J.P., Chen, L., Hasegawa, Y., Que Jr., L., Liu, A. Descri...

2012-05-02T18:57:12Z

New page: '''Unreleased structure''' The entry 4eri is ON HOLD Authors: Huo, L., Fielding, A. J. , Chen, Y. , Li, T., Iwaki, H., Hosler, J.P., Chen, L., Hasegawa, Y., Que Jr., L., Liu, A. Descri...

New page

'''Unreleased structure'''

The entry 4eri is ON HOLD

Authors: Huo, L., Fielding, A. J. , Chen, Y. , Li, T., Iwaki, H., Hosler, J.P., Chen, L., Hasegawa, Y., Que Jr., L., Liu, A.

Description: Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase