4exb - Revision history

OCA at 15:11, 14 March 2024

2024-03-14T15:11:14Z

←Older revision		Revision as of 15:11, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4exb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EXB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4exb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EXB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4exb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4exb OCA], [https://pdbe.org/4exb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4exb RCSB], [https://www.ebi.ac.uk/pdbsum/4exb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4exb ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.75Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4exb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4exb OCA], [https://pdbe.org/4exb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4exb RCSB], [https://www.ebi.ac.uk/pdbsum/4exb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4exb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q9HUH3_PSEAE Q9HUH3_PSEAE]		[https://www.uniprot.org/uniprot/Q9HUH3_PSEAE Q9HUH3_PSEAE]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Bacterial infections are increasingly difficult to treat owing to the spread of antibiotic resistance. A major concern is Gram-negative bacteria, for which the discovery of new antimicrobial drugs has been particularly scarce. In an effort to accelerate early steps in drug discovery, the EU-funded AEROPATH project aims to identify novel targets in the opportunistic pathogen Pseudomonas aeruginosa by applying a multidisciplinary approach encompassing target validation, structural characterization, assay development and hit identification from small-molecule libraries. Here, the strategies used for target selection are described and progress in protein production and structure analysis is reported. Of the 102 selected targets, 84 could be produced in soluble form and the de novo structures of 39 proteins have been determined. The crystal structures of eight of these targets, ranging from hypothetical unknown proteins to metabolic enzymes from different functional classes (PA1645, PA1648, PA2169, PA3770, PA4098, PA4485, PA4992 and PA5259), are reported here. The structural information is expected to provide a firm basis for the improvement of hit compounds identified from fragment-based and high-throughput screening campaigns.
-
-	The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery.,Moynie L, Schnell R, McMahon SA, Sandalova T, Boulkerou WA, Schmidberger JW, Alphey M, Cukier C, Duthie F, Kopec J, Liu H, Jacewicz A, Hunter WN, Naismith JH, Schneider G Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jan 1;69(Pt 1):25-34. doi:, 10.1107/S1744309112044739. Epub 2012 Dec 25. PMID:23295481<ref>PMID:23295481</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4exb" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:21, 7 October 2022

2022-10-07T04:21:25Z

←Older revision		Revision as of 04:21, 7 October 2022
Line 1:		Line 1:

	==Putative aldo-keto reductase from Pseudomona aeruginosa==		==Putative aldo-keto reductase from Pseudomona aeruginosa==
-	<StructureSection load='4exb' size='340' side='right' caption='[[4exb]], [[Resolution\|resolution]] 2.75Å' scene=''>	+	<StructureSection load='4exb' size='340' side='right'caption='[[4exb]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4exb]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EXB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4exb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EXB FirstGlance]. <br>
-	</td></tr>~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA4992 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4exb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4exb OCA], [https://pdbe.org/4exb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4exb RCSB], [https://www.ebi.ac.uk/pdbsum/4exb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4exb ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4exb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4exb OCA], [~~http~~://pdbe.org/4exb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4exb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4exb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4exb ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9HUH3_PSEAE Q9HUH3_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseae~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Sandalova, T~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: ~~Schneider, G~~]]	+	[[Category: Sandalova T]]
-	[[Category: ~~Schnell, R~~]]	+	[[Category: Schneider G]]
-	[[Category: ~~Aldo-keto reductase]]~~	+	[[Category: Schnell R]]
-	~~[[Category: Nadp+ binding]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Pseudomona~~]]	+

OCA at 02:54, 11 December 2016

2016-12-11T02:54:51Z

←Older revision		Revision as of 02:54, 11 December 2016
Line 1:		Line 1:
		+
	==Putative aldo-keto reductase from Pseudomona aeruginosa==		==Putative aldo-keto reductase from Pseudomona aeruginosa==
	<StructureSection load='4exb' size='340' side='right' caption='[[4exb]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='4exb' size='340' side='right' caption='[[4exb]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4exb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EXB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4exb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EXB FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA4992 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 ~~Pseudomonas aeruginosa PAO1~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA4992 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4exb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4exb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4exb RCSB], [http://www.ebi.ac.uk/pdbsum/4exb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4exb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4exb OCA], [http://pdbe.org/4exb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4exb RCSB], [http://www.ebi.ac.uk/pdbsum/4exb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4exb ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4exb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudomonas aeruginosa pao1~~]]	+	[[Category: Pseae]]
	[[Category: Sandalova, T]]		[[Category: Sandalova, T]]
	[[Category: Schneider, G]]		[[Category: Schneider, G]]

OCA at 17:04, 9 December 2014

2014-12-09T17:04:54Z

←Older revision		Revision as of 17:04, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4exb.~~png~~\|~~left\|200px~~]]	+	==Putative aldo-keto reductase from Pseudomona aeruginosa==
		+	<StructureSection load='4exb' size='340' side='right' caption='[[4exb]], [[Resolution\|resolution]] 2.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4exb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EXB FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA4992 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 Pseudomonas aeruginosa PAO1])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4exb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4exb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4exb RCSB], [http://www.ebi.ac.uk/pdbsum/4exb PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial infections are increasingly difficult to treat owing to the spread of antibiotic resistance. A major concern is Gram-negative bacteria, for which the discovery of new antimicrobial drugs has been particularly scarce. In an effort to accelerate early steps in drug discovery, the EU-funded AEROPATH project aims to identify novel targets in the opportunistic pathogen Pseudomonas aeruginosa by applying a multidisciplinary approach encompassing target validation, structural characterization, assay development and hit identification from small-molecule libraries. Here, the strategies used for target selection are described and progress in protein production and structure analysis is reported. Of the 102 selected targets, 84 could be produced in soluble form and the de novo structures of 39 proteins have been determined. The crystal structures of eight of these targets, ranging from hypothetical unknown proteins to metabolic enzymes from different functional classes (PA1645, PA1648, PA2169, PA3770, PA4098, PA4485, PA4992 and PA5259), are reported here. The structural information is expected to provide a firm basis for the improvement of hit compounds identified from fragment-based and high-throughput screening campaigns.

-	~~{{STRUCTURE_4exb\| PDB=4exb \| SCENE= }}~~	+	The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery.,Moynie L, Schnell R, McMahon SA, Sandalova T, Boulkerou WA, Schmidberger JW, Alphey M, Cukier C, Duthie F, Kopec J, Liu H, Jacewicz A, Hunter WN, Naismith JH, Schneider G Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jan 1;69(Pt 1):25-34. doi:, 10.1107/S1744309112044739. Epub 2012 Dec 25. PMID:23295481<ref>PMID:23295481</ref>

-	~~===Putative aldo-keto reductase from Pseudomona aeruginosa===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23295481}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4exb]] is a 6 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXB OCA].~~	+	</StructureSection>
	[[Category: Pseudomonas aeruginosa pao1]]		[[Category: Pseudomonas aeruginosa pao1]]
-	[[Category: Sandalova, T.]]	+	[[Category: Sandalova, T]]
-	[[Category: Schneider, G.]]	+	[[Category: Schneider, G]]
-	[[Category: Schnell, R.]]	+	[[Category: Schnell, R]]
	[[Category: Aldo-keto reductase]]		[[Category: Aldo-keto reductase]]
	[[Category: Nadp+ binding]]		[[Category: Nadp+ binding]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Pseudomona]]		[[Category: Pseudomona]]

OCA at 11:50, 23 January 2013

2013-01-23T11:50:18Z

←Older revision		Revision as of 11:50, 23 January 2013
Line 1:		Line 1:
-	[[Image:4exb.~~jpg~~\|left\|200px]]	+	[[Image:4exb.png\|left\|200px]]

	{{STRUCTURE_4exb\| PDB=4exb \| SCENE= }}		{{STRUCTURE_4exb\| PDB=4exb \| SCENE= }}
Line 5:		Line 5:
	===Putative aldo-keto reductase from Pseudomona aeruginosa===		===Putative aldo-keto reductase from Pseudomona aeruginosa===

		+	{{ABSTRACT_PUBMED_23295481}}

	==About this Structure==		==About this Structure==

OCA at 08:23, 9 January 2013

2013-01-09T08:23:30Z

←Older revision		Revision as of 08:23, 9 January 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4exb.jpg\|left\|200px]]

-	~~The entry~~ 4exb ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4exb\| PDB=4exb \| SCENE= }}

-	~~Authors: Schnell, R., Schneider, G., Sandalova, T.~~	+	===Putative aldo-keto reductase from Pseudomona aeruginosa===

-	~~Description~~: ~~Putative aldo~~-keto reductase ~~from~~ Pseudomona ~~aeruginosa~~	+
		+	==About this Structure==
		+	[[4exb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXB OCA].
		+	[[Category: Pseudomonas aeruginosa pao1]]
		+	[[Category: Sandalova, T.]]
		+	[[Category: Schneider, G.]]
		+	[[Category: Schnell, R.]]
		+	[[Category: Aldo-keto reductase]]
		+	[[Category: Nadp+ binding]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Pseudomona]]

OCA at 05:40, 23 May 2012

2012-05-23T05:40:10Z

←Older revision		Revision as of 05:40, 23 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4exb is ON HOLD	+	The entry 4exb is ON HOLD until Paper Publication

	Authors: Schnell, R., Schneider, G., Sandalova, T.		Authors: Schnell, R., Schneider, G., Sandalova, T.

	Description: Putative aldo-keto reductase from Pseudomona aeruginosa		Description: Putative aldo-keto reductase from Pseudomona aeruginosa

OCA at 08:51, 16 May 2012

2012-05-16T08:51:26Z

←Older revision		Revision as of 08:51, 16 May 2012
Line 3:		Line 3:
	The entry 4exb is ON HOLD		The entry 4exb is ON HOLD

-	Authors: Schnell, R, Schneider, G, Sandalova, T	+	Authors: Schnell, R., Schneider, G., Sandalova, T.

-	Description: Putative aldo-keto reductase from Pseudomona aeruginosa,	+	Description: Putative aldo-keto reductase from Pseudomona aeruginosa

OCA: Protected "4exb" [edit=sysop:move=sysop]

2012-05-09T08:52:12Z

Protected "4exb" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:52, 9 May 2012

OCA: New page: '''Unreleased structure''' The entry 4exb is ON HOLD Authors: Schnell, R, Schneider, G, Sandalova, T Description: Putative aldo-keto reductase from Pseudomona aeruginosa,

2012-05-09T08:52:12Z

New page: '''Unreleased structure''' The entry 4exb is ON HOLD Authors: Schnell, R, Schneider, G, Sandalova, T Description: Putative aldo-keto reductase from Pseudomona aeruginosa,

New page

'''Unreleased structure'''

The entry 4exb is ON HOLD

Authors: Schnell, R, Schneider, G, Sandalova, T

Description: Putative aldo-keto reductase from Pseudomona aeruginosa,