4f0e - Revision history

OCA at 19:23, 29 May 2024

2024-05-29T19:23:17Z

←Older revision		Revision as of 19:23, 29 May 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/PAR15_HUMAN PAR15_HUMAN] Transcriptional repressor. Has ADP-ribosyltransferase activity.		[https://www.uniprot.org/uniprot/PAR15_HUMAN PAR15_HUMAN] Transcriptional repressor. Has ADP-ribosyltransferase activity.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The diphtheria toxin-like ADP-ribosyltransferases (ARTDs) are an enzyme family that catalyzes the transfer of ADP-ribose units onto substrate proteins by using nicotinamide adenine dinucleotide (NAD(+)) as a cosubstrate. They have a documented role in chromatin remodelling and DNA repair, and inhibitors of ARTD1 and 2 (PARP1 and 2) are currently in clinical trials for the treatment of cancer. The detailed function of most other ARTDs is still unknown. By using virtual screening, we identified small ligands of ARTD7 (PARP15/BAL3) and ARTD8 (PARP14/BAL2). Thermal-shift assays confirmed that 16 compounds, belonging to eight structural classes, bound to ARTD7/ARTD8. Affinity measurements with isothermal titration calorimetry for two isomers of the most promising hit compound confirmed binding in the low micromolar range to ARTD8. Crystal structures showed anchoring of the hits in the nicotinamide pocket. These results form a starting point in the development of chemical tools for the study of the role and function of ARTD7 and ARTD8.
		+
		+	Discovery of Ligands for ADP-Ribosyltransferases via Docking-Based Virtual Screening.,Andersson CD, Karlberg T, Ekblad T, Lindgren AE, Thorsell AG, Spjut S, Uciechowska U, Niemiec MS, Wittung-Stafshede P, Weigelt J, Elofsson M, Schuler H, Linusson A J Med Chem. 2012 Aug 20. PMID:22823910<ref>PMID:22823910</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4f0e" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Poly(ADP-ribose) polymerase 3D structures\|Poly(ADP-ribose) polymerase 3D structures]]		*[[Poly(ADP-ribose) polymerase 3D structures\|Poly(ADP-ribose) polymerase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:12, 14 March 2024

2024-03-14T15:12:57Z

←Older revision		Revision as of 15:12, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4f0e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F0E FirstGlance]. <br>		<table><tr><td colspan='2'>[[4f0e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F0E FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0RU:8-METHYL-2-[(PYRIMIDIN-2-YLSULFANYL)METHYL]QUINAZOLIN-4(1H)-ONE'>0RU</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0RU:8-METHYL-2-[(PYRIMIDIN-2-YLSULFANYL)METHYL]QUINAZOLIN-4(1H)-ONE'>0RU</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f0e OCA], [https://pdbe.org/4f0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f0e RCSB], [https://www.ebi.ac.uk/pdbsum/4f0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f0e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f0e OCA], [https://pdbe.org/4f0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f0e RCSB], [https://www.ebi.ac.uk/pdbsum/4f0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f0e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/PAR15_HUMAN PAR15_HUMAN] Transcriptional repressor. Has ADP-ribosyltransferase activity.		[https://www.uniprot.org/uniprot/PAR15_HUMAN PAR15_HUMAN] Transcriptional repressor. Has ADP-ribosyltransferase activity.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The diphtheria toxin-like ADP-ribosyltransferases (ARTDs) are an enzyme family that catalyzes the transfer of ADP-ribose units onto substrate proteins by using nicotinamide adenine dinucleotide (NAD(+)) as a cosubstrate. They have a documented role in chromatin remodelling and DNA repair, and inhibitors of ARTD1 and 2 (PARP1 and 2) are currently in clinical trials for the treatment of cancer. The detailed function of most other ARTDs is still unknown. By using virtual screening, we identified small ligands of ARTD7 (PARP15/BAL3) and ARTD8 (PARP14/BAL2). Thermal-shift assays confirmed that 16 compounds, belonging to eight structural classes, bound to ARTD7/ARTD8. Affinity measurements with isothermal titration calorimetry for two isomers of the most promising hit compound confirmed binding in the low micromolar range to ARTD8. Crystal structures showed anchoring of the hits in the nicotinamide pocket. These results form a starting point in the development of chemical tools for the study of the role and function of ARTD7 and ARTD8.
-
-	Discovery of Ligands for ADP-Ribosyltransferases via Docking-Based Virtual Screening.,Andersson CD, Karlberg T, Ekblad T, Lindgren AE, Thorsell AG, Spjut S, Uciechowska U, Niemiec MS, Wittung-Stafshede P, Weigelt J, Elofsson M, Schuler H, Linusson A J Med Chem. 2012 Aug 20. PMID:22823910<ref>PMID:22823910</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4f0e" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Poly(ADP-ribose) polymerase 3D structures\|Poly(ADP-ribose) polymerase 3D structures]]		*[[Poly(ADP-ribose) polymerase 3D structures\|Poly(ADP-ribose) polymerase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:26, 7 October 2022

2022-10-07T04:26:01Z

←Older revision		Revision as of 04:26, 7 October 2022
Line 1:		Line 1:

	==Human ADP-RIBOSYLTRANSFERASE 7 (ARTD7/PARP15), CATALYTIC DOMAIN IN COMPLEX WITH STO1102==		==Human ADP-RIBOSYLTRANSFERASE 7 (ARTD7/PARP15), CATALYTIC DOMAIN IN COMPLEX WITH STO1102==
-	<StructureSection load='4f0e' size='340' side='right' caption='[[4f0e]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='4f0e' size='340' side='right'caption='[[4f0e]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f0e]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F0E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4F0E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f0e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F0E FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0RU:8-METHYL-2-[(PYRIMIDIN-2-YLSULFANYL)METHYL]QUINAZOLIN-4(1H)-ONE'>0RU</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0RU:8-METHYL-2-[(PYRIMIDIN-2-YLSULFANYL)METHYL]QUINAZOLIN-4(1H)-ONE'>0RU</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3blj\|3blj]], [[3gey\|3gey]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f0e OCA], [https://pdbe.org/4f0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f0e RCSB], [https://www.ebi.ac.uk/pdbsum/4f0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f0e ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAL3, PARP15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_ADP-ribosyltransferase NAD(+) ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.30 2.4.2.30] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f0e OCA], [~~http~~://pdbe.org/4f0e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4f0e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4f0e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4f0e ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PAR15_HUMAN PAR15_HUMAN]] Transcriptional repressor. Has ADP-ribosyltransferase activity.	+	[https://www.uniprot.org/uniprot/PAR15_HUMAN PAR15_HUMAN] Transcriptional repressor. Has ADP-ribosyltransferase activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 20:

	==See Also==		==See Also==
-	*[[Poly (ADP-ribose) polymerase\|Poly (ADP-ribose) polymerase]]	+	*[[Poly(ADP-ribose) polymerase 3D structures\|Poly(ADP-ribose) polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: Andersson~~, C D~~]]	+	[[Category: Large Structures]]
-	[[Category: Ekblad, T]]	+	[[Category: Andersson CD]]
-	[[Category: Elofsson, M]]	+	[[Category: Ekblad T]]
-	[[Category: Karlberg, T]]	+	[[Category: Elofsson M]]
-	[[Category: Lindgren, A]]	+	[[Category: Karlberg T]]
-	[[Category: Linusson, A]]	+	[[Category: Lindgren A]]
-	[[Category: Schuler, H]]	+	[[Category: Linusson A]]
-	[[Category: Spjut, S]]	+	[[Category: Schuler H]]
-	[[Category: Thorsell~~, A G~~]]	+	[[Category: Spjut S]]
-	[[Category: Weigelt, J]]	+	[[Category: Thorsell AG]]
-	~~[[Category: Adp-ribose]]~~	+	[[Category: Weigelt J]]
-	~~[[Category: Artd]]~~	+
-	~~[[Category: B-aggressive lymphoma protein 3]]~~	+
-	~~[[Category: Bal3]]~~	+
-	~~[[Category: Glycosyltransferase]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Parp]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulation]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Transferase-transferase inhibitor complex~~]]	+

OCA at 01:08, 5 August 2016

2016-08-05T01:08:08Z

←Older revision		Revision as of 01:08, 5 August 2016
Line 1:		Line 1:
		+
	==Human ADP-RIBOSYLTRANSFERASE 7 (ARTD7/PARP15), CATALYTIC DOMAIN IN COMPLEX WITH STO1102==		==Human ADP-RIBOSYLTRANSFERASE 7 (ARTD7/PARP15), CATALYTIC DOMAIN IN COMPLEX WITH STO1102==
	<StructureSection load='4f0e' size='340' side='right' caption='[[4f0e]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4f0e' size='340' side='right' caption='[[4f0e]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f0e]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F0E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F0E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f0e]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F0E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F0E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0RU:8-METHYL-2-[(PYRIMIDIN-2-YLSULFANYL)METHYL]QUINAZOLIN-4(1H)-ONE'>0RU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0RU:8-METHYL-2-[(PYRIMIDIN-2-YLSULFANYL)METHYL]QUINAZOLIN-4(1H)-ONE'>0RU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3blj\|3blj]], [[3gey\|3gey]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3blj\|3blj]], [[3gey\|3gey]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAL3, PARP15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAL3, PARP15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_ADP-ribosyltransferase NAD(+) ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.30 2.4.2.30] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_ADP-ribosyltransferase NAD(+) ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.30 2.4.2.30] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f0e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f0e RCSB], [http://www.ebi.ac.uk/pdbsum/4f0e PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f0e OCA], [http://pdbe.org/4f0e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f0e RCSB], [http://www.ebi.ac.uk/pdbsum/4f0e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f0e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4f0e" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Andersson, C D]]		[[Category: Andersson, C D]]
	[[Category: Ekblad, T]]		[[Category: Ekblad, T]]

OCA at 14:35, 25 December 2014

2014-12-25T14:35:31Z

←Older revision		Revision as of 14:35, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f0e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f0e RCSB], [http://www.ebi.ac.uk/pdbsum/4f0e PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f0e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f0e RCSB], [http://www.ebi.ac.uk/pdbsum/4f0e PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PAR15_HUMAN PAR15_HUMAN]] Transcriptional repressor. Has ADP-ribosyltransferase activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 16:17, 9 December 2014

2014-12-09T16:17:03Z

←Older revision		Revision as of 16:17, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4f0e.~~png~~\|~~left~~\|~~200px~~]]	+	==Human ADP-RIBOSYLTRANSFERASE 7 (ARTD7/PARP15), CATALYTIC DOMAIN IN COMPLEX WITH STO1102==
		+	<StructureSection load='4f0e' size='340' side='right' caption='[[4f0e]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4f0e]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F0E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F0E FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0RU:8-METHYL-2-[(PYRIMIDIN-2-YLSULFANYL)METHYL]QUINAZOLIN-4(1H)-ONE'>0RU</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3blj\|3blj]], [[3gey\|3gey]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAL3, PARP15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_ADP-ribosyltransferase NAD(+) ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.30 2.4.2.30] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f0e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f0e RCSB], [http://www.ebi.ac.uk/pdbsum/4f0e PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The diphtheria toxin-like ADP-ribosyltransferases (ARTDs) are an enzyme family that catalyzes the transfer of ADP-ribose units onto substrate proteins by using nicotinamide adenine dinucleotide (NAD(+)) as a cosubstrate. They have a documented role in chromatin remodelling and DNA repair, and inhibitors of ARTD1 and 2 (PARP1 and 2) are currently in clinical trials for the treatment of cancer. The detailed function of most other ARTDs is still unknown. By using virtual screening, we identified small ligands of ARTD7 (PARP15/BAL3) and ARTD8 (PARP14/BAL2). Thermal-shift assays confirmed that 16 compounds, belonging to eight structural classes, bound to ARTD7/ARTD8. Affinity measurements with isothermal titration calorimetry for two isomers of the most promising hit compound confirmed binding in the low micromolar range to ARTD8. Crystal structures showed anchoring of the hits in the nicotinamide pocket. These results form a starting point in the development of chemical tools for the study of the role and function of ARTD7 and ARTD8.

-	~~{{STRUCTURE_4f0e\| PDB=4f0e \| SCENE= }}~~	+	Discovery of Ligands for ADP-Ribosyltransferases via Docking-Based Virtual Screening.,Andersson CD, Karlberg T, Ekblad T, Lindgren AE, Thorsell AG, Spjut S, Uciechowska U, Niemiec MS, Wittung-Stafshede P, Weigelt J, Elofsson M, Schuler H, Linusson A J Med Chem. 2012 Aug 20. PMID:22823910<ref>PMID:22823910</ref>

-	~~===Human ADP-RIBOSYLTRANSFERASE 7 (ARTD7~~/~~PARP15)~~, ~~CATALYTIC DOMAIN IN COMPLEX WITH STO1102===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22823910}}~~	+	==See Also==
-		+	*[[Poly (ADP-ribose) polymerase\|Poly (ADP-ribose) polymerase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4f0e~~]] ~~is a 4 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F0E OCA].~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Andersson, C D.]]	+	[[Category: Andersson, C D]]
-	[[Category: Ekblad, T.]]	+	[[Category: Ekblad, T]]
-	[[Category: Elofsson, M.]]	+	[[Category: Elofsson, M]]
-	[[Category: Karlberg, T.]]	+	[[Category: Karlberg, T]]
-	[[Category: Lindgren, A.]]	+	[[Category: Lindgren, A]]
-	[[Category: Linusson, A.]]	+	[[Category: Linusson, A]]
-	[[Category: Schuler, H.]]	+	[[Category: Schuler, H]]
-	[[Category: Spjut, S.]]	+	[[Category: Spjut, S]]
-	[[Category: Thorsell, A G.]]	+	[[Category: Thorsell, A G]]
-	[[Category: Weigelt, J.]]	+	[[Category: Weigelt, J]]
	[[Category: Adp-ribose]]		[[Category: Adp-ribose]]
	[[Category: Artd]]		[[Category: Artd]]

OCA at 09:35, 31 October 2012

2012-10-31T09:35:42Z

←Older revision		Revision as of 09:35, 31 October 2012
Line 1:		Line 1:
-	[[Image:4f0e.~~jpg~~\|left\|200px]]	+	[[Image:4f0e.png\|left\|200px]]

	{{STRUCTURE_4f0e\| PDB=4f0e \| SCENE= }}		{{STRUCTURE_4f0e\| PDB=4f0e \| SCENE= }}

OCA at 05:25, 5 September 2012

2012-09-05T05:25:26Z

←Older revision		Revision as of 05:25, 5 September 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4f0e.jpg\|left\|200px]]

-	~~The entry~~ 4f0e ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4f0e\| PDB=4f0e \| SCENE= }}

-	~~Authors: Karlberg~~, ~~T., Andersson, C.D., Lindgren, A., Thorsell, A.G., Ekblad, T., Spjut, S., Weigelt, J., Elofsson, M., Linusson, A., Schuler, H.~~	+	===Human ADP-RIBOSYLTRANSFERASE 7 (ARTD7/PARP15), CATALYTIC DOMAIN IN COMPLEX WITH STO1102===

-	~~Description~~: ~~Human ADP~~-~~RIBOSYLTRANSFERASE 7 (ARTD7~~/~~PARP15)~~, ~~CATALYTIC DOMAIN IN COMPLEX WITH STO1102~~	+	{{ABSTRACT_PUBMED_22823910}}
		+
		+	==About this Structure==
		+	[[4f0e]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F0E OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Andersson, C D.]]
		+	[[Category: Ekblad, T.]]
		+	[[Category: Elofsson, M.]]
		+	[[Category: Karlberg, T.]]
		+	[[Category: Lindgren, A.]]
		+	[[Category: Linusson, A.]]
		+	[[Category: Schuler, H.]]
		+	[[Category: Spjut, S.]]
		+	[[Category: Thorsell, A G.]]
		+	[[Category: Weigelt, J.]]
		+	[[Category: Adp-ribose]]
		+	[[Category: Artd]]
		+	[[Category: B-aggressive lymphoma protein 3]]
		+	[[Category: Bal3]]
		+	[[Category: Glycosyltransferase]]
		+	[[Category: Nucleus]]
		+	[[Category: Parp]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription regulation]]
		+	[[Category: Transferase]]
		+	[[Category: Transferase-transferase inhibitor complex]]

OCA: Protected "4f0e" [edit=sysop:move=sysop]

2012-05-16T08:52:19Z

Protected "4f0e" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:52, 16 May 2012

OCA: New page: '''Unreleased structure''' The entry 4f0e is ON HOLD until Paper Publication Authors: Karlberg, T., Andersson, C.D., Lindgren, A., Thorsell, A.G., Ekblad, T., Spjut, S., Weigelt, J., El...

2012-05-16T08:52:18Z

New page: '''Unreleased structure''' The entry 4f0e is ON HOLD until Paper Publication Authors: Karlberg, T., Andersson, C.D., Lindgren, A., Thorsell, A.G., Ekblad, T., Spjut, S., Weigelt, J., El...

New page

'''Unreleased structure'''

The entry 4f0e is ON HOLD until Paper Publication

Authors: Karlberg, T., Andersson, C.D., Lindgren, A., Thorsell, A.G., Ekblad, T., Spjut, S., Weigelt, J., Elofsson, M., Linusson, A., Schuler, H.

Description: Human ADP-RIBOSYLTRANSFERASE 7 (ARTD7/PARP15), CATALYTIC DOMAIN IN COMPLEX WITH STO1102