4f28 - Revision history

OCA at 04:29, 7 October 2022

2022-10-07T04:29:35Z

←Older revision		Revision as of 04:29, 7 October 2022
Line 1:		Line 1:

	==The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly==		==The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly==
-	<StructureSection load='4f28' size='340' side='right' caption='[[4f28]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='4f28' size='340' side='right'caption='[[4f28]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f28]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F28 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4F28 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f28]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F28 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lpq\|3lpq]], [[3ew0\|3ew0]], [[2qh7\|2qh7]], [[4f1e\|4f1e]], [[4f2c\|4f2c]], [[4ezf\|4ezf]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f28 OCA], [https://pdbe.org/4f28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f28 RCSB], [https://www.ebi.ac.uk/pdbsum/4f28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f28 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C10orf70, CISD1, MDS029, ZCD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f28 OCA], [~~http~~://pdbe.org/4f28 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4f28 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4f28 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4f28 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CISD1_HUMAN CISD1_HUMAN]] Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions.<ref>PMID:17584744</ref> <ref>PMID:17766440</ref>	+	[https://www.uniprot.org/uniprot/CISD1_HUMAN CISD1_HUMAN] Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions.<ref>PMID:17584744</ref> <ref>PMID:17766440</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: Axelrod~~, H L~~]]	+	[[Category: Large Structures]]
-	[[Category: Baxter~~, E L~~]]	+	[[Category: Axelrod HL]]
-	[[Category: Cohen~~, A E~~]]	+	[[Category: Baxter EL]]
-	[[Category: Jennings~~, P A~~]]	+	[[Category: Cohen AE]]
-	[[Category: Nechushtai, R]]	+	[[Category: Jennings PA]]
-	[[Category: Onuchic~~, J N~~]]	+	[[Category: Nechushtai R]]
-	[[Category: Paddock~~, M L~~]]	+	[[Category: Onuchic JN]]
-	[[Category: Wang, C]]	+	[[Category: Paddock ML]]
-	[[Category: Zuris~~, J A]]~~	+	[[Category: Wang C]]
-	~~[[Category: 2fe-2s protein]]~~	+	[[Category: Zuris JA]]
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Mitochondrial outer membrane]]~~	+
-	~~[[Category: Protein frustration]]~~	+
-	~~[[Category: Signal-anchor]]~~	+
-	~~[[Category: Transmembrane metal binding protein~~]]	+

OCA at 10:30, 4 August 2016

2016-08-04T10:30:47Z

←Older revision		Revision as of 10:30, 4 August 2016
Line 1:		Line 1:
		+
	==The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly==		==The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly==
	<StructureSection load='4f28' size='340' side='right' caption='[[4f28]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='4f28' size='340' side='right' caption='[[4f28]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f28]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F28 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f28]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F28 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lpq\|3lpq]], [[3ew0\|3ew0]], [[2qh7\|2qh7]], [[4f1e\|4f1e]], [[4f2c\|4f2c]], [[4ezf\|4ezf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lpq\|3lpq]], [[3ew0\|3ew0]], [[2qh7\|2qh7]], [[4f1e\|4f1e]], [[4f2c\|4f2c]], [[4ezf\|4ezf]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C10orf70, CISD1, MDS029, ZCD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C10orf70, CISD1, MDS029, ZCD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f28 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f28 RCSB], [http://www.ebi.ac.uk/pdbsum/4f28 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f28 OCA], [http://pdbe.org/4f28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f28 RCSB], [http://www.ebi.ac.uk/pdbsum/4f28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f28 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4f28" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Axelrod, H L]]		[[Category: Axelrod, H L]]
	[[Category: Baxter, E L]]		[[Category: Baxter, E L]]

OCA at 08:21, 25 December 2014

2014-12-25T08:21:44Z

←Older revision		Revision as of 08:21, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f28 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f28 RCSB], [http://www.ebi.ac.uk/pdbsum/4f28 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f28 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f28 RCSB], [http://www.ebi.ac.uk/pdbsum/4f28 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CISD1_HUMAN CISD1_HUMAN]] Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions.<ref>PMID:17584744</ref> <ref>PMID:17766440</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:47, 9 December 2014

2014-12-09T15:47:08Z

←Older revision		Revision as of 15:47, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4f28.~~png~~\|~~left~~\|~~200px~~]]	+	==The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly==
		+	<StructureSection load='4f28' size='340' side='right' caption='[[4f28]], [[Resolution\|resolution]] 1.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4f28]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F28 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lpq\|3lpq]], [[3ew0\|3ew0]], [[2qh7\|2qh7]], [[4f1e\|4f1e]], [[4f2c\|4f2c]], [[4ezf\|4ezf]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C10orf70, CISD1, MDS029, ZCD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f28 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f28 RCSB], [http://www.ebi.ac.uk/pdbsum/4f28 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metalloproteins (MPs) comprise one-third of all known protein structures. This diverse set of proteins contain a plethora of unique inorganic moieties capable of performing chemistry that would otherwise be impossible using only the amino acids found in nature. Most of the well-studied MPs are generally viewed as being very rigid in structure, and it is widely thought that the properties of the metal centers are primarily determined by the small fraction of amino acids that make up the local environment. Here we examine both theoretically and experimentally whether distal regions can influence the metal center in the diabetes drug target mitoNEET. We demonstrate that a loop (L2) 20 A away from the metal center exerts allosteric control over the cluster binding domain and regulates multiple properties of the metal center. Mutagenesis of L2 results in significant shifts in the redox potential of the [2Fe-2S] cluster and orders of magnitude effects on the rate of [2Fe-2S] cluster transfer to an apo-acceptor protein. These surprising effects occur in the absence of any structural changes. An examination of the native basin dynamics of the protein using all-atom simulations shows that twisting in L2 controls scissoring in the cluster binding domain and results in perturbations to one of the cluster-coordinating histidines. These allosteric effects are in agreement with previous folding simulations that predicted L2 could communicate with residues surrounding the metal center. Our findings suggest that long-range dynamical changes in the protein backbone can have a significant effect on the functional properties of MPs.

-	~~{{STRUCTURE_4f28\| PDB=4f28 \| SCENE= }}~~	+	Allosteric control in a metalloprotein dramatically alters function.,Baxter EL, Zuris JA, Wang C, Vo PL, Axelrod HL, Cohen AE, Paddock ML, Nechushtai R, Onuchic JN, Jennings PA Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):948-53. doi:, 10.1073/pnas.1208286110. Epub 2012 Dec 27. PMID:23271805<ref>PMID:23271805</ref>

-	~~===The Crystal Structure~~ of ~~a Human MitoNEET mutant with Met 62 Replaced by a Gly===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23271805}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4f28]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F28 OCA].~~	+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Axelrod, H L.]]	+	[[Category: Axelrod, H L]]
-	[[Category: Baxter, E L.]]	+	[[Category: Baxter, E L]]
-	[[Category: Cohen, A E.]]	+	[[Category: Cohen, A E]]
-	[[Category: Jennings, P A.]]	+	[[Category: Jennings, P A]]
-	[[Category: Nechushtai, R.]]	+	[[Category: Nechushtai, R]]
-	[[Category: Onuchic, J N.]]	+	[[Category: Onuchic, J N]]
-	[[Category: Paddock, M L.]]	+	[[Category: Paddock, M L]]
-	[[Category: Wang, C.]]	+	[[Category: Wang, C]]
-	[[Category: Zuris, J A.]]	+	[[Category: Zuris, J A]]
	[[Category: 2fe-2s protein]]		[[Category: 2fe-2s protein]]
	[[Category: Membrane]]		[[Category: Membrane]]

OCA at 12:30, 30 January 2013

2013-01-30T12:30:35Z

←Older revision		Revision as of 12:30, 30 January 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4f28.png\|left\|200px]]

-	~~The entry~~ 4f28 ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4f28\| PDB=4f28 \| SCENE= }}

-	~~Authors: Baxter, E.L., Zuris, J.A., Wang, C., Axelrod, H.L., Cohen, A.E., Paddock, M.L., Nechushtai, R., Onuchic, J.N., Jennings, P.A.~~	+	===The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly===

-	~~Description: The Crystal~~ Structure of a ~~Human MitoNEET mutant~~ with ~~Met 62 Replaced by a Gly~~	+	{{ABSTRACT_PUBMED_23271805}}
		+
		+	==About this Structure==
		+	[[4f28]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F28 OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Axelrod, H L.]]
		+	[[Category: Baxter, E L.]]
		+	[[Category: Cohen, A E.]]
		+	[[Category: Jennings, P A.]]
		+	[[Category: Nechushtai, R.]]
		+	[[Category: Onuchic, J N.]]
		+	[[Category: Paddock, M L.]]
		+	[[Category: Wang, C.]]
		+	[[Category: Zuris, J A.]]
		+	[[Category: 2fe-2s protein]]
		+	[[Category: Membrane]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Mitochondrial outer membrane]]
		+	[[Category: Protein frustration]]
		+	[[Category: Signal-anchor]]
		+	[[Category: Transmembrane metal binding protein]]

OCA at 06:43, 25 July 2012

2012-07-25T06:43:49Z

←Older revision		Revision as of 06:43, 25 July 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4f28 is ON HOLD	+	The entry 4f28 is ON HOLD until Paper Publication

	Authors: Baxter, E.L., Zuris, J.A., Wang, C., Axelrod, H.L., Cohen, A.E., Paddock, M.L., Nechushtai, R., Onuchic, J.N., Jennings, P.A.		Authors: Baxter, E.L., Zuris, J.A., Wang, C., Axelrod, H.L., Cohen, A.E., Paddock, M.L., Nechushtai, R., Onuchic, J.N., Jennings, P.A.

	Description: The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly		Description: The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly

OCA: Protected "4f28" [edit=sysop:move=sysop]

2012-05-16T08:54:10Z

Protected "4f28" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:54, 16 May 2012

OCA: New page: '''Unreleased structure''' The entry 4f28 is ON HOLD Authors: Baxter, E.L., Zuris, J.A., Wang, C., Axelrod, H.L., Cohen, A.E., Paddock, M.L., Nechushtai, R., Onuchic, J.N., Jennings, P....

2012-05-16T08:54:09Z

New page: '''Unreleased structure''' The entry 4f28 is ON HOLD Authors: Baxter, E.L., Zuris, J.A., Wang, C., Axelrod, H.L., Cohen, A.E., Paddock, M.L., Nechushtai, R., Onuchic, J.N., Jennings, P....

New page

'''Unreleased structure'''

The entry 4f28 is ON HOLD

Authors: Baxter, E.L., Zuris, J.A., Wang, C., Axelrod, H.L., Cohen, A.E., Paddock, M.L., Nechushtai, R., Onuchic, J.N., Jennings, P.A.

Description: The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly