4fls - Revision history

OCA at 15:28, 14 March 2024

2024-03-14T15:28:22Z

←Older revision		Revision as of 15:28, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fls]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FLS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fls]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FLS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fls OCA], [https://pdbe.org/4fls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fls RCSB], [https://www.ebi.ac.uk/pdbsum/4fls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fls ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fls OCA], [https://pdbe.org/4fls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fls RCSB], [https://www.ebi.ac.uk/pdbsum/4fls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fls ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>		[https://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Iterative saturation mutagenesis and combinatorial active site saturation focused on vicinal amino acids were used to alter the acceptor specificity of amylosucrase from Neisseria polysaccharea , a sucrose-utilizing alpha-transglucosidase, and sort out improved variants. From the screening of three semirational sublibraries accounting in total for 20 000 variants, we report here the isolation of three double mutants of N. polysaccharea amylosucrase displaying a spectacular specificity enhancement toward both sucrose, the donor substrate, and the allyl 2-acetamido-2-deoxy-alpha-d-glucopyranoside acceptor as compared to the wild-type enzyme. Such levels of activity improvement have never been reported before for this class of carbohydrate-active enzymes. X-ray structure of the best performing enzymes supported by molecular dynamics simulations showed local rigidity of the -1 subsite as well as flexibility of loops involved in active site topology, which both account for the enhanced catalytic performances of the mutants. The study well illustrates the importance of taking into account the local conformation of catalytic residues as well as protein dynamics during the catalytic process, when designing enzyme libraries.
-
-	Applying pairwise combinations of amino Acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides.,Champion E, Guerin F, Moulis C, Barbe S, Tran TH, Morel S, Descroix K, Monsan P, Mourey L, Mulard LA, Tranier S, Remaud-Simeon M, Andre I J Am Chem Soc. 2012 Nov 14;134(45):18677-88. doi: 10.1021/ja306845b. Epub 2012, Oct 30. PMID:23072374<ref>PMID:23072374</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4fls" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:57, 19 October 2022

2022-10-19T19:57:15Z

←Older revision		Revision as of 19:57, 19 October 2022
Line 1:		Line 1:

	==Crystal structure of Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose.==		==Crystal structure of Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose.==
-	<StructureSection load='4fls' size='340' side='right' caption='[[4fls]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4fls' size='340' side='right'caption='[[4fls]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fls]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"neisseria_polysacchareae"_riou_et_al._1983 "neisseria polysacchareae" riou et al. 1983~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FLS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fls]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FLS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=~~GOL~~:~~GLYCEROL~~'>~~GOL~~</scene>, <scene name='pdbligand=~~SUC~~:~~SUCROSE~~'>~~SUC~~</scene><~~/td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat">[[1g5a\|1g5a]]~~, ~~[[4flo\|4flo]], [[4flq\|4flq]], [[4flr\|4flr]]~~<~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fls OCA], [https://pdbe.org/4fls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fls RCSB], [https://www.ebi.ac.uk/pdbsum/4fls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fls ProSAT]</span></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">ams ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=489 "Neisseria polysacchareae" Riou et al. 1983])</td></tr>~~	+
-	~~<tr id='activity~~'>~~<td class="sblockLbl"><b>Activity:~~</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amylosucrase Amylosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.4 2.4.1.4] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fls OCA], [~~http~~://pdbe.org/4fls PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fls RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fls PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fls ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO]] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>	+	[https://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Neisseria polysacchareae riou et al. 1983~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Amylosucrase~~]]	+	[[Category: Neisseria polysaccharea]]
-	[[Category: Andre, I]]	+	[[Category: Andre I]]
-	[[Category: Barbe, S]]	+	[[Category: Barbe S]]
-	[[Category: Champion, E]]	+	[[Category: Champion E]]
-	[[Category: Descroix, K]]	+	[[Category: Descroix K]]
-	[[Category: Guerin, F]]	+	[[Category: Guerin F]]
-	[[Category: Monsan, P]]	+	[[Category: Monsan P]]
-	[[Category: Morel, S]]	+	[[Category: Morel S]]
-	[[Category: Moulis, C]]	+	[[Category: Moulis C]]
-	[[Category: Mourey, L]]	+	[[Category: Mourey L]]
-	[[Category: Mulard~~, L A~~]]	+	[[Category: Mulard LA]]
-	[[Category: Remaud-Simeon, M]]	+	[[Category: Remaud-Simeon M]]
-	[[Category: Tran~~, T H~~]]	+	[[Category: Tran TH]]
-	[[Category: Tranier, S]]	+	[[Category: Tranier S]]
-	~~[[Category: Amylose synthesis]]~~	+
-	~~[[Category: Beta/alpha-barrel]]~~	+
-	~~[[Category: Glucosyltransferase carbohydrate]]~~	+
-	~~[[Category: Glycoside hydrolase]]~~	+
-	~~[[Category: Sucrose isomerization]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 19:25, 5 August 2016

2016-08-05T19:25:24Z

←Older revision		Revision as of 19:25, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose.==		==Crystal structure of Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose.==
	<StructureSection load='4fls' size='340' side='right' caption='[[4fls]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4fls' size='340' side='right' caption='[[4fls]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fls]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Neisseria_polysaccharea Neisseria polysaccharea~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FLS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fls]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"neisseria_polysacchareae"_riou_et_al._1983 "neisseria polysacchareae" riou et al. 1983]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FLS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g5a\|1g5a]], [[4flo\|4flo]], [[4flq\|4flq]], [[4flr\|4flr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g5a\|1g5a]], [[4flo\|4flo]], [[4flq\|4flq]], [[4flr\|4flr]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ams ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=489 Neisseria ~~polysaccharea~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ams ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=489 "Neisseria polysacchareae" Riou et al. 1983])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amylosucrase Amylosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.4 2.4.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amylosucrase Amylosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.4 2.4.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fls OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fls RCSB], [http://www.ebi.ac.uk/pdbsum/4fls PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fls OCA], [http://pdbe.org/4fls PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fls RCSB], [http://www.ebi.ac.uk/pdbsum/4fls PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fls ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4fls" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Neisseria polysacchareae riou et al. 1983]]
	[[Category: Amylosucrase]]		[[Category: Amylosucrase]]
-	[[Category: Neisseria polysaccharea]]
	[[Category: Andre, I]]		[[Category: Andre, I]]
	[[Category: Barbe, S]]		[[Category: Barbe, S]]

OCA at 05:12, 25 December 2014

2014-12-25T05:12:08Z

←Older revision		Revision as of 05:12, 25 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4fls.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose.==
		+	<StructureSection load='4fls' size='340' side='right' caption='[[4fls]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4fls]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FLS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g5a\|1g5a]], [[4flo\|4flo]], [[4flq\|4flq]], [[4flr\|4flr]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ams ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=489 Neisseria polysaccharea])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amylosucrase Amylosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.4 2.4.1.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fls OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fls RCSB], [http://www.ebi.ac.uk/pdbsum/4fls PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO]] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Iterative saturation mutagenesis and combinatorial active site saturation focused on vicinal amino acids were used to alter the acceptor specificity of amylosucrase from Neisseria polysaccharea , a sucrose-utilizing alpha-transglucosidase, and sort out improved variants. From the screening of three semirational sublibraries accounting in total for 20 000 variants, we report here the isolation of three double mutants of N. polysaccharea amylosucrase displaying a spectacular specificity enhancement toward both sucrose, the donor substrate, and the allyl 2-acetamido-2-deoxy-alpha-d-glucopyranoside acceptor as compared to the wild-type enzyme. Such levels of activity improvement have never been reported before for this class of carbohydrate-active enzymes. X-ray structure of the best performing enzymes supported by molecular dynamics simulations showed local rigidity of the -1 subsite as well as flexibility of loops involved in active site topology, which both account for the enhanced catalytic performances of the mutants. The study well illustrates the importance of taking into account the local conformation of catalytic residues as well as protein dynamics during the catalytic process, when designing enzyme libraries.

-	~~{{STRUCTURE_4fls\| PDB=4fls \| SCENE= }}~~	+	Applying pairwise combinations of amino Acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides.,Champion E, Guerin F, Moulis C, Barbe S, Tran TH, Morel S, Descroix K, Monsan P, Mourey L, Mulard LA, Tranier S, Remaud-Simeon M, Andre I J Am Chem Soc. 2012 Nov 14;134(45):18677-88. doi: 10.1021/ja306845b. Epub 2012, Oct 30. PMID:23072374<ref>PMID:23072374</ref>

-	~~===Crystal structure~~ of ~~Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23072374}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4fls]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLS OCA].~~	+	</StructureSection>
	[[Category: Amylosucrase]]		[[Category: Amylosucrase]]
	[[Category: Neisseria polysaccharea]]		[[Category: Neisseria polysaccharea]]
-	[[Category: Andre, I.]]	+	[[Category: Andre, I]]
-	[[Category: Barbe, S.]]	+	[[Category: Barbe, S]]
-	[[Category: Champion, E.]]	+	[[Category: Champion, E]]
-	[[Category: Descroix, K.]]	+	[[Category: Descroix, K]]
-	[[Category: Guerin, F.]]	+	[[Category: Guerin, F]]
-	[[Category: Monsan, P.]]	+	[[Category: Monsan, P]]
-	[[Category: Morel, S.]]	+	[[Category: Morel, S]]
-	[[Category: Moulis, C.]]	+	[[Category: Moulis, C]]
-	[[Category: Mourey, L.]]	+	[[Category: Mourey, L]]
-	[[Category: Mulard, L A.]]	+	[[Category: Mulard, L A]]
-	[[Category: Remaud-Simeon, M.]]	+	[[Category: Remaud-Simeon, M]]
-	[[Category: Tran, T H.]]	+	[[Category: Tran, T H]]
-	[[Category: Tranier, S.]]	+	[[Category: Tranier, S]]
	[[Category: Amylose synthesis]]		[[Category: Amylose synthesis]]
	[[Category: Beta/alpha-barrel]]		[[Category: Beta/alpha-barrel]]

OCA at 08:18, 2 January 2013

2013-01-02T08:18:01Z

←Older revision		Revision as of 08:18, 2 January 2013
Line 1:		Line 1:
-	[[Image:4fls.~~jpg~~\|left\|200px]]	+	[[Image:4fls.png\|left\|200px]]

	{{STRUCTURE_4fls\| PDB=4fls \| SCENE= }}		{{STRUCTURE_4fls\| PDB=4fls \| SCENE= }}

OCA at 09:07, 31 October 2012

2012-10-31T09:07:27Z

←Older revision		Revision as of 09:07, 31 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4fls.jpg\|left\|200px]]

-	~~The entry~~ 4fls ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4fls\| PDB=4fls \| SCENE= }}

-	~~Authors: Guerin, F., Champion, E., Moulis, C., Barbe, S., Tran, T.H., Morel, S., Descroix, K., Monsan, P., Mulard, L.A., Remaud~~-~~Simeon, M., Andre, I., Mourey, L., Tranier, S~~.	+	===Crystal structure of Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose.===

-	~~Description: Crystal~~ structure ~~of Amylosucrase inactive double mutant F290K-E328Q~~ from Neisseria polysaccharea ~~in complex with sucrose~~.	+	{{ABSTRACT_PUBMED_23072374}}
		+
		+	==About this Structure==
		+	[[4fls]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLS OCA].
		+	[[Category: Amylosucrase]]
		+	[[Category: Neisseria polysaccharea]]
		+	[[Category: Andre, I.]]
		+	[[Category: Barbe, S.]]
		+	[[Category: Champion, E.]]
		+	[[Category: Descroix, K.]]
		+	[[Category: Guerin, F.]]
		+	[[Category: Monsan, P.]]
		+	[[Category: Morel, S.]]
		+	[[Category: Moulis, C.]]
		+	[[Category: Mourey, L.]]
		+	[[Category: Mulard, L A.]]
		+	[[Category: Remaud-Simeon, M.]]
		+	[[Category: Tran, T H.]]
		+	[[Category: Tranier, S.]]
		+	[[Category: Amylose synthesis]]
		+	[[Category: Beta/alpha-barrel]]
		+	[[Category: Glucosyltransferase carbohydrate]]
		+	[[Category: Glycoside hydrolase]]
		+	[[Category: Sucrose isomerization]]
		+	[[Category: Transferase]]

OCA: Protected "4fls" [edit=sysop:move=sysop]

2012-06-27T05:26:09Z

Protected "4fls" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:26, 27 June 2012

OCA: New page: '''Unreleased structure''' The entry 4fls is ON HOLD until Paper Publication Authors: Guerin, F., Champion, E., Moulis, C., Barbe, S., Tran, T.H., Morel, S., Descroix, K., Monsan, P., M...

2012-06-27T05:26:09Z

New page: '''Unreleased structure''' The entry 4fls is ON HOLD until Paper Publication Authors: Guerin, F., Champion, E., Moulis, C., Barbe, S., Tran, T.H., Morel, S., Descroix, K., Monsan, P., M...

New page

'''Unreleased structure'''

The entry 4fls is ON HOLD until Paper Publication

Authors: Guerin, F., Champion, E., Moulis, C., Barbe, S., Tran, T.H., Morel, S., Descroix, K., Monsan, P., Mulard, L.A., Remaud-Simeon, M., Andre, I., Mourey, L., Tranier, S.

Description: Crystal structure of Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose.