4fyg - Revision history

OCA at 15:36, 14 March 2024

2024-03-14T15:36:12Z

←Older revision		Revision as of 15:36, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fyg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FYG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fyg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FYG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PT:(2R)-3-{[(S)-HYDROXY{[(1S,2R,3R,4S,5S,6S)-2,3,6-TRIHYDROXY-4,5-BIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}PROPANE-1,2-DIYL+DIBUTANOATE'>3PT</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.822Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PT:(2R)-3-{[(S)-HYDROXY{[(1S,2R,3R,4S,5S,6S)-2,3,6-TRIHYDROXY-4,5-BIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}PROPANE-1,2-DIYL+DIBUTANOATE'>3PT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyg OCA], [https://pdbe.org/4fyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fyg RCSB], [https://www.ebi.ac.uk/pdbsum/4fyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyg OCA], [https://pdbe.org/4fyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fyg RCSB], [https://www.ebi.ac.uk/pdbsum/4fyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q5ZSD5_LEGPH Q5ZSD5_LEGPH]		[https://www.uniprot.org/uniprot/Q5ZSD5_LEGPH Q5ZSD5_LEGPH]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Legionella pneumophila is an opportunistic intracellular pathogen that causes sporadic and epidemic cases of Legionnaires' disease. Emerging data suggest that Legionella infection involves the subversion of host phosphoinositide (PI) metabolism. However, how this bacterium actively manipulates PI lipids to benefit its infection is still an enigma. Here, we report that the L. pneumophila virulence factor SidF is a phosphatidylinositol polyphosphate 3-phosphatase that specifically hydrolyzes the D3 phosphate of PI(3,4)P(2) and PI(3,4,5)P(3). This activity is necessary for anchoring of PI(4)P-binding effectors to bacterial phagosomes. Crystal structures of SidF and its complex with its substrate PI(3,4)P(2) reveal striking conformational rearrangement of residues at the catalytic site to form a cationic pocket that specifically accommodates the D4 phosphate group of the substrate. Thus, our findings unveil a unique Legionella PI phosphatase essential for the establishment of lipid identity of bacterial phagosomes.
-
-	Structural basis for substrate recognition by a unique Legionella phosphoinositide phosphatase.,Hsu F, Zhu W, Brennan L, Tao L, Luo ZQ, Mao Y Proc Natl Acad Sci U S A. 2012 Aug 7. PMID:22872863<ref>PMID:22872863</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4fyg" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 20:15, 19 October 2022

2022-10-19T20:15:23Z

←Older revision		Revision as of 20:15, 19 October 2022
Line 1:		Line 1:

	==Structural basis for substrate recognition by a novel Legionella phosphoinositide phosphatase==		==Structural basis for substrate recognition by a novel Legionella phosphoinositide phosphatase==
-	<StructureSection load='4fyg' size='340' side='right' caption='[[4fyg]], [[Resolution\|resolution]] 2.82Å' scene=''>	+	<StructureSection load='4fyg' size='340' side='right'caption='[[4fyg]], [[Resolution\|resolution]] 2.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fyg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Legph Legph~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FYG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fyg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FYG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PT:(2R)-3-{[(S)-HYDROXY{[(1S,2R,3R,4S,5S,6S)-2,3,6-TRIHYDROXY-4,5-BIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}PROPANE-1,2-DIYL+DIBUTANOATE'>3PT</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PT:(2R)-3-{[(S)-HYDROXY{[(1S,2R,3R,4S,5S,6S)-2,3,6-TRIHYDROXY-4,5-BIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}PROPANE-1,2-DIYL+DIBUTANOATE'>3PT</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fye\|4fye]], [[4fyf\|4fyf]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyg OCA], [https://pdbe.org/4fyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fyg RCSB], [https://www.ebi.ac.uk/pdbsum/4fyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyg ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg2584, sidF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol-3,4,5-trisphosphate_3-phosphatase Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.67 3.1.3.67] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyg OCA], [~~http~~://pdbe.org/4fyg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fyg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fyg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyg ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5ZSD5_LEGPH Q5ZSD5_LEGPH]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Legph~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase~~]]	+	[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
-	[[Category: Brennan, L]]	+	[[Category: Brennan L]]
-	[[Category: Hsu~~, F S~~]]	+	[[Category: Hsu FS]]
-	[[Category: Luo~~, Z Q~~]]	+	[[Category: Luo ZQ]]
-	[[Category: Mao, Y]]	+	[[Category: Mao Y]]
-	[[Category: Tao, L]]	+	[[Category: Tao L]]
-	[[Category: Zhu, W]]	+	[[Category: Zhu W]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Mixed alpha-beta]]~~	+
-	~~[[Category: Phosphoinositide phosphatase]]~~	+
-	~~[[Category: Phosphoinositide~~]]	+

OCA at 12:39, 11 August 2016

2016-08-11T12:39:01Z

←Older revision		Revision as of 12:39, 11 August 2016
Line 1:		Line 1:
		+
	==Structural basis for substrate recognition by a novel Legionella phosphoinositide phosphatase==		==Structural basis for substrate recognition by a novel Legionella phosphoinositide phosphatase==
	<StructureSection load='4fyg' size='340' side='right' caption='[[4fyg]], [[Resolution\|resolution]] 2.82Å' scene=''>		<StructureSection load='4fyg' size='340' side='right' caption='[[4fyg]], [[Resolution\|resolution]] 2.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fyg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Legionella_pneumophila_subsp._pneumophila Legionella pneumophila subsp. pneumophila~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FYG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fyg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FYG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PT:(2R)-3-{[(S)-HYDROXY{[(1S,2R,3R,4S,5S,6S)-2,3,6-TRIHYDROXY-4,5-BIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}PROPANE-1,2-DIYL+DIBUTANOATE'>3PT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PT:(2R)-3-{[(S)-HYDROXY{[(1S,2R,3R,4S,5S,6S)-2,3,6-TRIHYDROXY-4,5-BIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}PROPANE-1,2-DIYL+DIBUTANOATE'>3PT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fye\|4fye]], [[4fyf\|4fyf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fye\|4fye]], [[4fyf\|4fyf]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg2584, sidF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~91891 Legionella pneumophila subsp. pneumophila~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg2584, sidF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol-3,4,5-trisphosphate_3-phosphatase Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.67 3.1.3.67] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol-3,4,5-trisphosphate_3-phosphatase Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.67 3.1.3.67] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fyg RCSB], [http://www.ebi.ac.uk/pdbsum/4fyg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyg OCA], [http://pdbe.org/4fyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fyg RCSB], [http://www.ebi.ac.uk/pdbsum/4fyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4fyg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Legionella pneumophila subsp. pneumophila~~]]	+	[[Category: Legph]]
	[[Category: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase]]		[[Category: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase]]
	[[Category: Brennan, L]]		[[Category: Brennan, L]]

OCA at 12:37, 4 January 2015

2015-01-04T12:37:07Z

←Older revision		Revision as of 12:37, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fyg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila Legionella pneumophila subsp. pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FYG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fyg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila Legionella pneumophila subsp. pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FYG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PT:(2R)-3-{[(S)-HYDROXY{[(1S,2R,3R,4S,5S,6S)-2,3,6-TRIHYDROXY-4,5-BIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}PROPANE-1,2-DIYL+DIBUTANOATE'>3PT</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PT:(2R)-3-{[(S)-HYDROXY{[(1S,2R,3R,4S,5S,6S)-2,3,6-TRIHYDROXY-4,5-BIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}PROPANE-1,2-DIYL+DIBUTANOATE'>3PT</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fye\|4fye]], [[4fyf\|4fyf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fye\|4fye]], [[4fyf\|4fyf]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg2584, sidF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=91891 Legionella pneumophila subsp. pneumophila])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg2584, sidF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=91891 Legionella pneumophila subsp. pneumophila])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol-3,4,5-trisphosphate_3-phosphatase Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.67 3.1.3.67] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol-3,4,5-trisphosphate_3-phosphatase Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.67 3.1.3.67] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fyg RCSB], [http://www.ebi.ac.uk/pdbsum/4fyg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fyg RCSB], [http://www.ebi.ac.uk/pdbsum/4fyg PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	[[Category: Legionella pneumophila subsp. pneumophila]]		[[Category: Legionella pneumophila subsp. pneumophila]]
	[[Category: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase]]		[[Category: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase]]
-	[[Category: Brennan, L.]]	+	[[Category: Brennan, L]]
-	[[Category: Hsu, F S.]]	+	[[Category: Hsu, F S]]
-	[[Category: Luo, Z Q.]]	+	[[Category: Luo, Z Q]]
-	[[Category: Mao, Y.]]	+	[[Category: Mao, Y]]
-	[[Category: Tao, L.]]	+	[[Category: Tao, L]]
-	[[Category: Zhu, W.]]	+	[[Category: Zhu, W]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Membrane]]		[[Category: Membrane]]

OCA at 09:18, 11 June 2014

2014-06-11T09:18:55Z

←Older revision		Revision as of 09:18, 11 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4fyg.~~png~~\|~~left~~\|~~200px~~]]	+	==Structural basis for substrate recognition by a novel Legionella phosphoinositide phosphatase==
		+	<StructureSection load='4fyg' size='340' side='right' caption='[[4fyg]], [[Resolution\|resolution]] 2.82Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4fyg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila Legionella pneumophila subsp. pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FYG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PT:(2R)-3-{[(S)-HYDROXY{[(1S,2R,3R,4S,5S,6S)-2,3,6-TRIHYDROXY-4,5-BIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}PROPANE-1,2-DIYL+DIBUTANOATE'>3PT</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fye\|4fye]], [[4fyf\|4fyf]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg2584, sidF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=91891 Legionella pneumophila subsp. pneumophila])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol-3,4,5-trisphosphate_3-phosphatase Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.67 3.1.3.67] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fyg RCSB], [http://www.ebi.ac.uk/pdbsum/4fyg PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Legionella pneumophila is an opportunistic intracellular pathogen that causes sporadic and epidemic cases of Legionnaires' disease. Emerging data suggest that Legionella infection involves the subversion of host phosphoinositide (PI) metabolism. However, how this bacterium actively manipulates PI lipids to benefit its infection is still an enigma. Here, we report that the L. pneumophila virulence factor SidF is a phosphatidylinositol polyphosphate 3-phosphatase that specifically hydrolyzes the D3 phosphate of PI(3,4)P(2) and PI(3,4,5)P(3). This activity is necessary for anchoring of PI(4)P-binding effectors to bacterial phagosomes. Crystal structures of SidF and its complex with its substrate PI(3,4)P(2) reveal striking conformational rearrangement of residues at the catalytic site to form a cationic pocket that specifically accommodates the D4 phosphate group of the substrate. Thus, our findings unveil a unique Legionella PI phosphatase essential for the establishment of lipid identity of bacterial phagosomes.

-	~~{{STRUCTURE_4fyg\| PDB=4fyg \| SCENE= }}~~	+	Structural basis for substrate recognition by a unique Legionella phosphoinositide phosphatase.,Hsu F, Zhu W, Brennan L, Tao L, Luo ZQ, Mao Y Proc Natl Acad Sci U S A. 2012 Aug 7. PMID:22872863<ref>PMID:22872863</ref>

-	~~===Structural basis for substrate recognition by~~ a ~~novel Legionella phosphoinositide phosphatase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22872863}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[4fyg]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Legionella_pneumophila_subsp~~.~~_pneumophila Legionella pneumophila subsp. pneumophila]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4FYG OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022872863<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Legionella pneumophila subsp. pneumophila]]		[[Category: Legionella pneumophila subsp. pneumophila]]
	[[Category: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase]]		[[Category: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase]]

OCA at 05:50, 5 September 2012

2012-09-05T05:50:57Z

←Older revision		Revision as of 05:50, 5 September 2012
Line 1:		Line 1:
-	[[Image:4fyg.~~jpg~~\|left\|200px]]	+	[[Image:4fyg.png\|left\|200px]]

	{{STRUCTURE_4fyg\| PDB=4fyg \| SCENE= }}		{{STRUCTURE_4fyg\| PDB=4fyg \| SCENE= }}

OCA at 05:53, 22 August 2012

2012-08-22T05:53:31Z

←Older revision		Revision as of 05:53, 22 August 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4fyg.jpg\|left\|200px]]

-	~~The entry~~ 4fyg ~~is ON HOLD~~	+	{{STRUCTURE_4fyg\| PDB=4fyg \| SCENE= }}

-	~~Authors: Hsu, F.S., Zhu, W., Brennan, L., Tao, L., Luo, Z.Q., Mao, Y.~~	+	===Structural basis for substrate recognition by a novel Legionella phosphoinositide phosphatase===

-	~~Description: Structural basis for substrate recognition by~~ a ~~novel~~ Legionella ~~phosphoinositide~~ phosphatase	+	{{ABSTRACT_PUBMED_22872863}}
		+
		+	==About this Structure==
		+	[[4fyg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila Legionella pneumophila subsp. pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYG OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022872863</ref><references group="xtra"/>
		+	[[Category: Legionella pneumophila subsp. pneumophila]]
		+	[[Category: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase]]
		+	[[Category: Brennan, L.]]
		+	[[Category: Hsu, F S.]]
		+	[[Category: Luo, Z Q.]]
		+	[[Category: Mao, Y.]]
		+	[[Category: Tao, L.]]
		+	[[Category: Zhu, W.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Membrane]]
		+	[[Category: Mixed alpha-beta]]
		+	[[Category: Phosphoinositide phosphatase]]
		+	[[Category: Phosphoinositide]]

OCA at 05:05, 18 July 2012

2012-07-18T05:05:32Z

←Older revision		Revision as of 05:05, 18 July 2012
Line 3:		Line 3:
	The entry 4fyg is ON HOLD		The entry 4fyg is ON HOLD

-	Authors: Hsu, F.S., Zhu, W ., Brennan, L. , Tao, L. , Luo, Z.Q. , Mao, Y.	+	Authors: Hsu, F.S., Zhu, W., Brennan, L., Tao, L., Luo, Z.Q., Mao, Y.

	Description: Structural basis for substrate recognition by a novel Legionella phosphoinositide phosphatase		Description: Structural basis for substrate recognition by a novel Legionella phosphoinositide phosphatase

OCA: Protected "4fyg" [edit=sysop:move=sysop]

2012-07-11T07:53:39Z

Protected "4fyg" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:53, 11 July 2012

OCA: New page: '''Unreleased structure''' The entry 4fyg is ON HOLD Authors: Hsu, F.S., Zhu, W ., Brennan, L. , Tao, L. , Luo, Z.Q. , Mao, Y. Description: Structural basis for substrate recognition b...

2012-07-11T07:53:38Z

New page: '''Unreleased structure''' The entry 4fyg is ON HOLD Authors: Hsu, F.S., Zhu, W ., Brennan, L. , Tao, L. , Luo, Z.Q. , Mao, Y. Description: Structural basis for substrate recognition b...

New page

'''Unreleased structure'''

The entry 4fyg is ON HOLD

Authors: Hsu, F.S., Zhu, W ., Brennan, L. , Tao, L. , Luo, Z.Q. , Mao, Y.

Description: Structural basis for substrate recognition by a novel Legionella phosphoinositide phosphatase