4fzw - Revision history

OCA at 11:24, 1 March 2024

2024-03-01T11:24:23Z

←Older revision		Revision as of 11:24, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fzw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FZW FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fzw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FZW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzw OCA], [https://pdbe.org/4fzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fzw RCSB], [https://www.ebi.ac.uk/pdbsum/4fzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fzw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzw OCA], [https://pdbe.org/4fzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fzw RCSB], [https://www.ebi.ac.uk/pdbsum/4fzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fzw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/PAAG_ECOLI PAAG_ECOLI] Catalyzes the reversible conversion of the epoxide to 2-oxepin-2(3H)-ylideneacetyl-CoA (oxepin-CoA).<ref>PMID:12846838</ref> <ref>PMID:20660314</ref> <ref>PMID:9748275</ref>		[https://www.uniprot.org/uniprot/PAAG_ECOLI PAAG_ECOLI] Catalyzes the reversible conversion of the epoxide to 2-oxepin-2(3H)-ylideneacetyl-CoA (oxepin-CoA).<ref>PMID:12846838</ref> <ref>PMID:20660314</ref> <ref>PMID:9748275</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Microbial anaerobic and so-called hybrid pathways for degradation of aromatic compounds contain beta-oxidation-like steps. These reactions convert the product of the opening of the aromatic ring to common metabolites. The hybrid phenylacetate degradation pathway is encoded in Escherichia coli by the paa operon containing genes for 10 enzymes. Previously, we have analyzed protein-protein interactions among the enzymes catalyzing the initial oxidation steps in the paa pathway (Grishin, A. M., Ajamian, E., Tao, L., Zhang, L., Menard, R., and Cygler, M. (2011) J. Biol. Chem. 286, 10735-10743). Here we report characterization of interactions between the remaining enzymes of this pathway and show another stable complex, PaaFG, an enoyl-CoA hydratase and enoyl-Coa isomerase, both belonging to the crotonase superfamily. These steps are biochemically similar to the well studied fatty acid beta-oxidation, which can be catalyzed by individual monofunctional enzymes, multifunctional enzymes comprising several domains, or enzymatic complexes such as the bacterial fatty acid beta-oxidation complex. We have determined the structure of the PaaFG complex and determined that although individually PaaF and PaaG are similar to enzymes from the fatty acid beta-oxidation pathway, the structure of the complex is dissimilar from bacterial fatty acid beta-oxidation complexes. The PaaFG complex has a four-layered structure composed of homotrimeric discs of PaaF and PaaG. The active sites of PaaF and PaaG are adapted to accept the intermediary components of the Paa pathway, different from those of the fatty acid beta-oxidation. The association of PaaF and PaaG into a stable complex might serve to speed up the steps of the pathway following the conversion of phenylacetyl-CoA to a toxic and unstable epoxide-CoA by PaaABCE monooxygenase.
-
-	Protein-protein interactions in the beta-oxidation part of the phenylacetate utilization pathway: crystal structure of the PaaF-PaaG hydratase-isomerase complex.,Grishin AM, Ajamian E, Zhang L, Rouiller I, Bostina M, Cygler M J Biol Chem. 2012 Nov 2;287(45):37986-96. doi: 10.1074/jbc.M112.388231. Epub 2012, Sep 7. PMID:22961985<ref>PMID:22961985</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4fzw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 20:17, 19 October 2022

2022-10-19T20:17:00Z

←Older revision		Revision as of 20:17, 19 October 2022
Line 1:		Line 1:

	==Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli==		==Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli==
-	<StructureSection load='4fzw' size='340' side='right' caption='[[4fzw]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='4fzw' size='340' side='right'caption='[[4fzw]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fzw]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FZW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fzw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FZW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1393, JW1388, paaF, ydbR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), paaG, ydbT, b1394, JW1389 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzw OCA], [https://pdbe.org/4fzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fzw RCSB], [https://www.ebi.ac.uk/pdbsum/4fzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fzw ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-CoA_hydratase Enoyl-CoA hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.17 4.2.1.17] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzw OCA], [~~http~~://pdbe.org/4fzw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fzw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fzw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fzw ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/PAAF_ECOLI PAAF_ECOLI]] Catalyzes the reversible conversion of enzymatically produced 2,3-dehydroadipyl-CoA into 3-hydroxyadipyl-CoA.<ref>PMID:20660314</ref> <ref>PMID:9748275</ref> [[http://www.uniprot.org/uniprot/PAAG_ECOLI PAAG_ECOLI]] Catalyzes the reversible conversion of the epoxide to 2-oxepin-2(3H)-ylideneacetyl-CoA (oxepin-CoA).<ref>PMID:12846838</ref> <ref>PMID:20660314</ref> <ref>PMID:9748275</ref>	+	[https://www.uniprot.org/uniprot/PAAG_ECOLI PAAG_ECOLI] Catalyzes the reversible conversion of the epoxide to 2-oxepin-2(3H)-ylideneacetyl-CoA (oxepin-CoA).<ref>PMID:12846838</ref> <ref>PMID:20660314</ref> <ref>PMID:9748275</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4fzw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4fzw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Enoyl-CoA hydratase 3D structures\|Enoyl-CoA hydratase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Enoyl~~-~~CoA hydratase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Structural genomic~~]]	+	[[Category: Cygler M]]
-	[[Category: Cygler, M]]	+	[[Category: Grishin AM]]
-	[[Category: Grishin~~, A M]]~~	+
-	~~[[Category: Bsgi]]~~	+
-	~~[[Category: Crotonase fold]]~~	+
-	~~[[Category: Enzymes of the phenylacetate degradation pathway]]~~	+
-	~~[[Category: Isomerase-lyase complex~~]]	+

OCA at 08:23, 15 November 2017

2017-11-15T08:23:35Z

←Older revision		Revision as of 08:23, 15 November 2017
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4fzw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4fzw" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Enoyl-CoA hydratase\|Enoyl-CoA hydratase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:12, 5 August 2016

2016-08-05T14:12:28Z

←Older revision		Revision as of 14:12, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli==		==Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli==
	<StructureSection load='4fzw' size='340' side='right' caption='[[4fzw]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='4fzw' size='340' side='right' caption='[[4fzw]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fzw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FZW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fzw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FZW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1393, JW1388, paaF, ydbR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~]), paaG, ydbT, b1394, JW1389 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1393, JW1388, paaF, ydbR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), paaG, ydbT, b1394, JW1389 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-CoA_hydratase Enoyl-CoA hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.17 4.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-CoA_hydratase Enoyl-CoA hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.17 4.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fzw RCSB], [http://www.ebi.ac.uk/pdbsum/4fzw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzw OCA], [http://pdbe.org/4fzw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fzw RCSB], [http://www.ebi.ac.uk/pdbsum/4fzw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fzw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4fzw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Enoyl-CoA hydratase]]		[[Category: Enoyl-CoA hydratase]]
-	[[Category: Escherichia coli k-12]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Cygler, M]]		[[Category: Cygler, M]]

OCA at 03:09, 25 December 2014

2014-12-25T03:09:43Z

←Older revision		Revision as of 03:09, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fzw RCSB], [http://www.ebi.ac.uk/pdbsum/4fzw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fzw RCSB], [http://www.ebi.ac.uk/pdbsum/4fzw PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PAAF_ECOLI PAAF_ECOLI]] Catalyzes the reversible conversion of enzymatically produced 2,3-dehydroadipyl-CoA into 3-hydroxyadipyl-CoA.<ref>PMID:20660314</ref> <ref>PMID:9748275</ref> [[http://www.uniprot.org/uniprot/PAAG_ECOLI PAAG_ECOLI]] Catalyzes the reversible conversion of the epoxide to 2-oxepin-2(3H)-ylideneacetyl-CoA (oxepin-CoA).<ref>PMID:12846838</ref> <ref>PMID:20660314</ref> <ref>PMID:9748275</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 16:16, 9 December 2014

2014-12-09T16:16:48Z

←Older revision		Revision as of 16:16, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4fzw.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli==
		+	<StructureSection load='4fzw' size='340' side='right' caption='[[4fzw]], [[Resolution\|resolution]] 2.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4fzw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FZW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1393, JW1388, paaF, ydbR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12]), paaG, ydbT, b1394, JW1389 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-CoA_hydratase Enoyl-CoA hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.17 4.2.1.17] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fzw RCSB], [http://www.ebi.ac.uk/pdbsum/4fzw PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microbial anaerobic and so-called hybrid pathways for degradation of aromatic compounds contain beta-oxidation-like steps. These reactions convert the product of the opening of the aromatic ring to common metabolites. The hybrid phenylacetate degradation pathway is encoded in Escherichia coli by the paa operon containing genes for 10 enzymes. Previously, we have analyzed protein-protein interactions among the enzymes catalyzing the initial oxidation steps in the paa pathway (Grishin, A. M., Ajamian, E., Tao, L., Zhang, L., Menard, R., and Cygler, M. (2011) J. Biol. Chem. 286, 10735-10743). Here we report characterization of interactions between the remaining enzymes of this pathway and show another stable complex, PaaFG, an enoyl-CoA hydratase and enoyl-Coa isomerase, both belonging to the crotonase superfamily. These steps are biochemically similar to the well studied fatty acid beta-oxidation, which can be catalyzed by individual monofunctional enzymes, multifunctional enzymes comprising several domains, or enzymatic complexes such as the bacterial fatty acid beta-oxidation complex. We have determined the structure of the PaaFG complex and determined that although individually PaaF and PaaG are similar to enzymes from the fatty acid beta-oxidation pathway, the structure of the complex is dissimilar from bacterial fatty acid beta-oxidation complexes. The PaaFG complex has a four-layered structure composed of homotrimeric discs of PaaF and PaaG. The active sites of PaaF and PaaG are adapted to accept the intermediary components of the Paa pathway, different from those of the fatty acid beta-oxidation. The association of PaaF and PaaG into a stable complex might serve to speed up the steps of the pathway following the conversion of phenylacetyl-CoA to a toxic and unstable epoxide-CoA by PaaABCE monooxygenase.

-	~~{{STRUCTURE_4fzw\| PDB=4fzw \| SCENE= }}~~	+	Protein-protein interactions in the beta-oxidation part of the phenylacetate utilization pathway: crystal structure of the PaaF-PaaG hydratase-isomerase complex.,Grishin AM, Ajamian E, Zhang L, Rouiller I, Bostina M, Cygler M J Biol Chem. 2012 Nov 2;287(45):37986-96. doi: 10.1074/jbc.M112.388231. Epub 2012, Sep 7. PMID:22961985<ref>PMID:22961985</ref>

-	~~===Crystal Structure~~ of the ~~PaaF-PaaG Hydratase-Isomerase Complex from E~~.~~coli===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22961985}}~~	+	==See Also==
-		+	*[[Enoyl-CoA hydratase\|Enoyl-CoA hydratase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4fzw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k~~-~~12 Escherichia coli k~~-12]~~. Full crystallographic information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=4FZW OCA].~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Enoyl-CoA hydratase]]		[[Category: Enoyl-CoA hydratase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
-	[[Category: ~~BSGI, Montreal-Kingston Bacterial~~ Structural ~~Genomics Initiative.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Cygler, M.]]	+	[[Category: Cygler, M]]
-	[[Category: Grishin, A M.]]	+	[[Category: Grishin, A M]]
	[[Category: Bsgi]]		[[Category: Bsgi]]
	[[Category: Crotonase fold]]		[[Category: Crotonase fold]]
	[[Category: Enzymes of the phenylacetate degradation pathway]]		[[Category: Enzymes of the phenylacetate degradation pathway]]
	[[Category: Isomerase-lyase complex]]		[[Category: Isomerase-lyase complex]]
-	[[Category: Montreal-kingston bacterial structural genomics initiative]]
-	[[Category: Structural genomic]]

OCA at 08:59, 16 January 2013

2013-01-16T08:59:43Z

←Older revision		Revision as of 08:59, 16 January 2013
Line 1:		Line 1:
-	[[Image:4fzw.~~jpg~~\|left\|200px]]	+	[[Image:4fzw.png\|left\|200px]]

	{{STRUCTURE_4fzw\| PDB=4fzw \| SCENE= }}		{{STRUCTURE_4fzw\| PDB=4fzw \| SCENE= }}
Line 5:		Line 5:
	===Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli===		===Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli===

		+	{{ABSTRACT_PUBMED_22961985}}

	==About this Structure==		==About this Structure==
-	[[4fzw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli~~ Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZW OCA].	+	[[4fzw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZW OCA].
	[[Category: Enoyl-CoA hydratase]]		[[Category: Enoyl-CoA hydratase]]
-	[[Category: Escherichia coli]]	+	[[Category: Escherichia coli k-12]]
	[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]		[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
	[[Category: Cygler, M.]]		[[Category: Cygler, M.]]

OCA at 07:22, 19 September 2012

2012-09-19T07:22:57Z

←Older revision		Revision as of 07:22, 19 September 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4fzw.jpg\|left\|200px]]

-	~~The entry~~ 4fzw ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4fzw\| PDB=4fzw \| SCENE= }}

-	~~Authors: Grishin, A~~.~~M., Cygler, M., Montreal-Kingston Bacterial Structural Genomics Initiative (BSGI)~~	+	===Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli===

-	~~Description: Crystal~~ Structure of the ~~PaaF~~-~~PaaG Hydratase~~-~~Isomerase Complex from E.coli~~	+
		+	==About this Structure==
		+	[[4fzw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZW OCA].
		+	[[Category: Enoyl-CoA hydratase]]
		+	[[Category: Escherichia coli]]
		+	[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
		+	[[Category: Cygler, M.]]
		+	[[Category: Grishin, A M.]]
		+	[[Category: Bsgi]]
		+	[[Category: Crotonase fold]]
		+	[[Category: Enzymes of the phenylacetate degradation pathway]]
		+	[[Category: Isomerase-lyase complex]]
		+	[[Category: Montreal-kingston bacterial structural genomics initiative]]
		+	[[Category: Structural genomic]]

OCA at 04:43, 29 August 2012

2012-08-29T04:43:08Z

←Older revision		Revision as of 04:43, 29 August 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4fzw is ON HOLD	+	The entry 4fzw is ON HOLD until Paper Publication

	Authors: Grishin, A.M., Cygler, M., Montreal-Kingston Bacterial Structural Genomics Initiative (BSGI)		Authors: Grishin, A.M., Cygler, M., Montreal-Kingston Bacterial Structural Genomics Initiative (BSGI)

	Description: Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli		Description: Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli

OCA: Protected "4fzw" [edit=sysop:move=sysop]

2012-07-18T05:06:29Z

Protected "4fzw" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:06, 18 July 2012