4g7a - Revision history

OCA at 20:28, 19 October 2022

2022-10-19T20:28:51Z

←Older revision		Revision as of 20:28, 19 October 2022
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	==The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1==		==The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1==
-	<StructureSection load='4g7a' size='340' side='right' caption='[[4g7a]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='4g7a' size='340' side='right'caption='[[4g7a]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g7a]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Sulsy Sulsy~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4G7A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g7a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurihydrogenibium_sp._YO3AOP1 Sulfurihydrogenibium sp. YO3AOP1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G7A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kop\|1kop]], [[1ca2\|1ca2]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7a OCA], [https://pdbe.org/4g7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g7a RCSB], [https://www.ebi.ac.uk/pdbsum/4g7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g7a ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYO3AOP1_0578 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=436114 SULSY])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4g7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7a OCA], [~~http~~://pdbe.org/4g7a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4g7a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4g7a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4g7a ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B2V8E3_SULSY B2V8E3_SULSY]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[Carbonic anhydrase\|Carbonic anhydrase]]	+	*[[Carbonic anhydrase 3D structures\|Carbonic anhydrase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Carbonate dehydratase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Sulsy]]~~	+	[[Category: Sulfurihydrogenibium sp. YO3AOP1]]
-	~~[[Category: Fiore, A Di~~]]	+	[[Category: De Simone G]]
-	[[Category: Simone, G De]]	+	[[Category: Di Fiore A]]
-	[[Category: ~~Lyase~~]]	+

OCA at 01:32, 5 August 2016

2016-08-05T01:32:29Z

←Older revision		Revision as of 01:32, 5 August 2016
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		+
	==The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1==		==The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1==
	<StructureSection load='4g7a' size='340' side='right' caption='[[4g7a]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4g7a' size='340' side='right' caption='[[4g7a]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g7a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfurihydrogenibium_sp._yo3aop1 Sulfurihydrogenibium sp. yo3aop1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G7A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g7a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulsy Sulsy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G7A FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kop\|1kop]], [[1ca2\|1ca2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kop\|1kop]], [[1ca2\|1ca2]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYO3AOP1_0578 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=436114 ~~Sulfurihydrogenibium sp. YO3AOP1~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYO3AOP1_0578 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=436114 SULSY])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g7a RCSB], [http://www.ebi.ac.uk/pdbsum/4g7a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7a OCA], [http://pdbe.org/4g7a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g7a RCSB], [http://www.ebi.ac.uk/pdbsum/4g7a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g7a ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4g7a" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Carbonate dehydratase]]		[[Category: Carbonate dehydratase]]
-	[[Category: ~~Sulfurihydrogenibium sp. yo3aop1~~]]	+	[[Category: Sulsy]]
	[[Category: Fiore, A Di]]		[[Category: Fiore, A Di]]
	[[Category: Simone, G De]]		[[Category: Simone, G De]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 10:57, 21 December 2014

2014-12-21T10:57:40Z

←Older revision		Revision as of 10:57, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4g7a\| PDB=4g7a \| SCENE= }}~~	+	==The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1==
-	===The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1===	+	<StructureSection load='4g7a' size='340' side='right' caption='[[4g7a]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23695259}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4g7a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfurihydrogenibium_sp._yo3aop1 Sulfurihydrogenibium sp. yo3aop1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G7A FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kop\|1kop]], [[1ca2\|1ca2]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYO3AOP1_0578 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=436114 Sulfurihydrogenibium sp. YO3AOP1])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g7a RCSB], [http://www.ebi.ac.uk/pdbsum/4g7a PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SspCA, a novel `extremo-alpha-carbonic anhydrase' isolated from the thermophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1, is an efficient catalyst for the hydration of CO2 and presents exceptional thermostability. Indeed, SspCA retains a high catalytic activity even after being heated to 343-373 K for several hours. Here, the crystallographic structure of this alpha-carbonic anhydrase (alpha-CA) is reported and the factors responsible for its function at high temperature are elucidated. In particular, the study suggests that increased structural compactness, together with an increased number of charged residues on the protein surface and a greater number of ionic networks, seem to be the key factors involved in the higher thermostability of this enzyme with respect to its mesophilic homologues. These findings are of extreme importance, since they provide a structural basis for the understanding of the mechanisms responsible for thermal stability in the alpha-CA family for the first time. The data obtained offer a tool that can be exploited to engineer alpha-CAs in order to obtain enzymes with enhanced thermostability for use in the harsh conditions of the CO2 capture and sequestration processes.

-	~~==About this Structure==~~	+	X-ray structure of the first `extremo-alpha-carbonic anhydrase', a dimeric enzyme from the thermophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1.,Di Fiore A, Capasso C, De Luca V, Monti SM, Carginale V, Supuran CT, Scozzafava A, Pedone C, Rossi M, De Simone G Acta Crystallogr D Biol Crystallogr. 2013 Jun;69(Pt 6):1150-9. doi:, 10.1107/S0907444913007208. Epub 2013 May 16. PMID:23695259<ref>PMID:23695259</ref>
-	~~[[4g7a]] is~~ a ~~2 chain structure with sequence~~ from ~~[http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Sulfurihydrogenibium_sp~~.~~_yo3aop1 Sulfurihydrogenibium sp~~. ~~yo3aop1]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4G7A OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:023695259</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+
		+	==See Also==
		+	*[[Carbonic anhydrase\|Carbonic anhydrase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Carbonate dehydratase]]		[[Category: Carbonate dehydratase]]
	[[Category: Sulfurihydrogenibium sp. yo3aop1]]		[[Category: Sulfurihydrogenibium sp. yo3aop1]]
-	[[Category: Fiore, A Di.]]	+	[[Category: Fiore, A Di]]
-	[[Category: Simone, G De.]]	+	[[Category: Simone, G De]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 15:24, 19 June 2013

2013-06-19T15:24:41Z

←Older revision		Revision as of 15:24, 19 June 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4g7a\| PDB=4g7a \| SCENE= }}
		+	===The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1===
		+	{{ABSTRACT_PUBMED_23695259}}

-	~~The entry~~ 4g7a is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4g7a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfurihydrogenibium_sp._yo3aop1 Sulfurihydrogenibium sp. yo3aop1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7A OCA].

-	~~Authors~~: Di Fiore, A.~~, De~~ Simone, G.	+	==Reference==
-		+	<ref group="xtra">PMID:023695259</ref><references group="xtra"/><references/>
-	~~Description~~: ~~The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1~~	+	[[Category: Carbonate dehydratase]]
		+	[[Category: Sulfurihydrogenibium sp. yo3aop1]]
		+	[[Category: Fiore, A Di.]]
		+	[[Category: Simone, G De.]]
		+	[[Category: Lyase]]

OCA at 07:03, 24 October 2012

2012-10-24T07:03:49Z

←Older revision		Revision as of 07:03, 24 October 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4g7a is ON HOLD	+	The entry 4g7a is ON HOLD until Paper Publication

	Authors: Di Fiore, A., De Simone, G.		Authors: Di Fiore, A., De Simone, G.

	Description: The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1		Description: The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1

OCA at 04:58, 8 August 2012

2012-08-08T04:58:17Z

←Older revision		Revision as of 04:58, 8 August 2012
Line 5:		Line 5:
	Authors: Di Fiore, A., De Simone, G.		Authors: Di Fiore, A., De Simone, G.

-	Description: The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium ~~yellostonense~~ YO3AOP1	+	Description: The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1

OCA: Protected "4g7a" [edit=sysop:move=sysop]

2012-08-01T05:29:28Z

Protected "4g7a" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:29, 1 August 2012

OCA: New page: '''Unreleased structure''' The entry 4g7a is ON HOLD Authors: Di Fiore, A., De Simone, G. Description: The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacte...

2012-08-01T05:29:27Z

New page: '''Unreleased structure''' The entry 4g7a is ON HOLD Authors: Di Fiore, A., De Simone, G. Description: The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacte...

New page

'''Unreleased structure'''

The entry 4g7a is ON HOLD

Authors: Di Fiore, A., De Simone, G.

Description: The crystal structure of an alpha Carbonic Anhydrase from the extremophilic bacterium Sulfurihydrogenibium yellostonense YO3AOP1