4g91 - Revision history

OCA at 15:41, 14 March 2024

2024-03-14T15:41:21Z

←Older revision		Revision as of 15:41, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4g91]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans] and [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G91 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G91 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4g91]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans] and [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G91 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G91 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g91 OCA], [https://pdbe.org/4g91 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g91 RCSB], [https://www.ebi.ac.uk/pdbsum/4g91 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g91 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g91 OCA], [https://pdbe.org/4g91 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g91 RCSB], [https://www.ebi.ac.uk/pdbsum/4g91 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g91 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/P87249_EMEND P87249_EMEND]		[https://www.uniprot.org/uniprot/P87249_EMEND P87249_EMEND]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The CCAAT box is a frequent element of eukaryotic promoters, and its specific recognition by the conserved heterotrimeric CCAAT-binding complex (CBC) constitutes a key step in promoter organization and regulation of transcription. Here, we report the crystal structures of the CBC from Aspergillus nidulans in the absence and in complex with double-stranded DNA at 1.8 A resolution. The histone-like subunits HapC and HapE induce nucleosome-like DNA bending by interacting with the sugar-phosphate backbone. Minor groove sensing and widening by subunit HapB tightly anchor the CBC to the CCAAT box, as shown by structural and biochemical data. Furthermore, crucial interactions of the DNA duplex with subunit HapB provide an explanation for the sequence specificity of the CBC. The herein-described mode of transcription factor binding answers the question of how histone proteins gained sequence specificity for the CCAAT box.
-
-	DNA minor groove sensing and widening by the CCAAT-binding complex.,Huber EM, Scharf DH, Hortschansky P, Groll M, Brakhage AA Structure. 2012 Oct 10;20(10):1757-68. doi: 10.1016/j.str.2012.07.012. Epub 2012 , Aug 16. PMID:22902862<ref>PMID:22902862</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4g91" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:43, 26 October 2022

2022-10-26T06:43:14Z

←Older revision		Revision as of 06:43, 26 October 2022
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	==CCAAT-binding complex from Aspergillus nidulans==		==CCAAT-binding complex from Aspergillus nidulans==
-	<StructureSection load='4g91' size='340' side='right' caption='[[4g91]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='4g91' size='340' side='right'caption='[[4g91]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g91]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~A._nidulans A.~~ nidulans] and [~~http~~://en.wikipedia.org/wiki/~~Aspergillus_nidulans~~ Aspergillus nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G91 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4G91 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g91]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans] and [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G91 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G91 FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1n1j\|1n1j]], [[4g92\|4g92]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g91 OCA], [https://pdbe.org/4g91 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g91 RCSB], [https://www.ebi.ac.uk/pdbsum/4g91 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g91 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hapB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=162425 A. nidulans]), AN4034.2, ANIA_04034 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans]), AN6492.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4g91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g91 OCA], [~~http~~://pdbe.org/4g91 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4g91 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4g91 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4g91 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/P87249_EMEND P87249_EMEND]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: A. nidulans]]
	[[Category: Aspergillus nidulans]]		[[Category: Aspergillus nidulans]]
-	[[Category: ~~Brakhage, A A~~]]	+	[[Category: Aspergillus nidulans FGSC A4]]
-	[[Category: ~~Groll, M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Hortschansky, P~~]]	+	[[Category: Brakhage AA]]
-	[[Category: ~~Huber, E~~ M]]	+	[[Category: Groll M]]
-	[[Category: ~~Scharf, D H~~]]	+	[[Category: Hortschansky P]]
-	[[Category: ~~Ccaat-binding complex~~]]	+	[[Category: Huber EM]]
-	[[Category: ~~Histone fold motif]]~~	+	[[Category: Scharf DH]]
-	~~[[Category: Minor groove binding]]~~	+
-	~~[[Category: Nucleosome]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Specific binding to the ccaat-box]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription factor~~]]	+

OCA at 11:01, 11 August 2016

2016-08-11T11:01:03Z

←Older revision		Revision as of 11:01, 11 August 2016
Line 1:		Line 1:
		+
	==CCAAT-binding complex from Aspergillus nidulans==		==CCAAT-binding complex from Aspergillus nidulans==
	<StructureSection load='4g91' size='340' side='right' caption='[[4g91]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4g91' size='340' side='right' caption='[[4g91]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1n1j\|1n1j]], [[4g92\|4g92]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1n1j\|1n1j]], [[4g92\|4g92]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hapB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=162425 A. nidulans]), AN4034.2, ANIA_04034 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans]), AN6492.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hapB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=162425 A. nidulans]), AN4034.2, ANIA_04034 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans]), AN6492.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g91 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g91 RCSB], [http://www.ebi.ac.uk/pdbsum/4g91 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g91 OCA], [http://pdbe.org/4g91 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g91 RCSB], [http://www.ebi.ac.uk/pdbsum/4g91 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g91 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4g91" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:46, 4 January 2015

2015-01-04T12:46:24Z

←Older revision		Revision as of 12:46, 4 January 2015
Line 1:		Line 1:
-	[[~~Image:~~4g91.~~jpg~~\|~~left~~\|~~200px~~]]	+	==CCAAT-binding complex from Aspergillus nidulans==
		+	<StructureSection load='4g91' size='340' side='right' caption='[[4g91]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4g91]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/A._nidulans A. nidulans] and [http://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G91 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G91 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1n1j\|1n1j]], [[4g92\|4g92]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hapB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=162425 A. nidulans]), AN4034.2, ANIA_04034 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans]), AN6492.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g91 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g91 RCSB], [http://www.ebi.ac.uk/pdbsum/4g91 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The CCAAT box is a frequent element of eukaryotic promoters, and its specific recognition by the conserved heterotrimeric CCAAT-binding complex (CBC) constitutes a key step in promoter organization and regulation of transcription. Here, we report the crystal structures of the CBC from Aspergillus nidulans in the absence and in complex with double-stranded DNA at 1.8 A resolution. The histone-like subunits HapC and HapE induce nucleosome-like DNA bending by interacting with the sugar-phosphate backbone. Minor groove sensing and widening by subunit HapB tightly anchor the CBC to the CCAAT box, as shown by structural and biochemical data. Furthermore, crucial interactions of the DNA duplex with subunit HapB provide an explanation for the sequence specificity of the CBC. The herein-described mode of transcription factor binding answers the question of how histone proteins gained sequence specificity for the CCAAT box.

-	~~{{STRUCTURE_4g91\| PDB=4g91 \| SCENE= }}~~	+	DNA minor groove sensing and widening by the CCAAT-binding complex.,Huber EM, Scharf DH, Hortschansky P, Groll M, Brakhage AA Structure. 2012 Oct 10;20(10):1757-68. doi: 10.1016/j.str.2012.07.012. Epub 2012 , Aug 16. PMID:22902862<ref>PMID:22902862</ref>

-	~~===CCAAT-binding complex from Aspergillus nidulans===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22902862}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4g91]] is a 3 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Aspergillus_nidulans_fgsc_a4 Aspergillus nidulans fgsc a4] and [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G91 OCA].	+	</StructureSection>
-	[[Category: ~~Aspergillus~~ nidulans ~~fgsc a4~~]]	+	[[Category: A. nidulans]]
-	[[Category: ~~Emericella~~ nidulans]]	+	[[Category: Aspergillus nidulans]]
-	[[Category: Brakhage, A A.]]	+	[[Category: Brakhage, A A]]
-	[[Category: Groll, M.]]	+	[[Category: Groll, M]]
-	[[Category: Hortschansky, P.]]	+	[[Category: Hortschansky, P]]
-	[[Category: Huber, E M.]]	+	[[Category: Huber, E M]]
-	[[Category: Scharf, D H.]]	+	[[Category: Scharf, D H]]
	[[Category: Ccaat-binding complex]]		[[Category: Ccaat-binding complex]]
	[[Category: Histone fold motif]]		[[Category: Histone fold motif]]

OCA at 09:06, 31 October 2012

2012-10-31T09:06:11Z

←Older revision		Revision as of 09:06, 31 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4g91.jpg\|left\|200px]]

-	~~The entry~~ 4g91 ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4g91\| PDB=4g91 \| SCENE= }}

-	~~Authors: Huber, E.M., Scharf, D.H., Hortschansky, P., Groll, M., Brakhage, A.A.~~	+	===CCAAT-binding complex from Aspergillus nidulans===

-	~~Description~~: ~~CCAAT-binding complex~~ from Aspergillus nidulans	+	{{ABSTRACT_PUBMED_22902862}}
		+
		+	==About this Structure==
		+	[[4g91]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_nidulans_fgsc_a4 Aspergillus nidulans fgsc a4] and [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G91 OCA].
		+	[[Category: Aspergillus nidulans fgsc a4]]
		+	[[Category: Emericella nidulans]]
		+	[[Category: Brakhage, A A.]]
		+	[[Category: Groll, M.]]
		+	[[Category: Hortschansky, P.]]
		+	[[Category: Huber, E M.]]
		+	[[Category: Scharf, D H.]]
		+	[[Category: Ccaat-binding complex]]
		+	[[Category: Histone fold motif]]
		+	[[Category: Minor groove binding]]
		+	[[Category: Nucleosome]]
		+	[[Category: Nucleus]]
		+	[[Category: Specific binding to the ccaat-box]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription factor]]

OCA at 05:31, 22 August 2012

2012-08-22T05:31:50Z

←Older revision		Revision as of 05:31, 22 August 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4g91 is ON HOLD	+	The entry 4g91 is ON HOLD until Paper Publication

	Authors: Huber, E.M., Scharf, D.H., Hortschansky, P., Groll, M., Brakhage, A.A.		Authors: Huber, E.M., Scharf, D.H., Hortschansky, P., Groll, M., Brakhage, A.A.

	Description: CCAAT-binding complex from Aspergillus nidulans		Description: CCAAT-binding complex from Aspergillus nidulans

OCA: Protected "4g91" [edit=sysop:move=sysop]

2012-08-01T05:31:21Z

Protected "4g91" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:31, 1 August 2012

OCA: New page: '''Unreleased structure''' The entry 4g91 is ON HOLD Authors: Huber, E.M., Scharf, D.H., Hortschansky, P., Groll, M., Brakhage, A.A. Description: CCAAT-binding complex from Aspergillus...

2012-08-01T05:31:20Z

New page: '''Unreleased structure''' The entry 4g91 is ON HOLD Authors: Huber, E.M., Scharf, D.H., Hortschansky, P., Groll, M., Brakhage, A.A. Description: CCAAT-binding complex from Aspergillus...

New page

'''Unreleased structure'''

The entry 4g91 is ON HOLD

Authors: Huber, E.M., Scharf, D.H., Hortschansky, P., Groll, M., Brakhage, A.A.

Description: CCAAT-binding complex from Aspergillus nidulans