4gde - Revision history

OCA at 15:44, 14 March 2024

2024-03-14T15:44:03Z

←Older revision		Revision as of 15:44, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gde]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GDE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gde]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GDE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gde OCA], [https://pdbe.org/4gde PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gde RCSB], [https://www.ebi.ac.uk/pdbsum/4gde PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gde ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gde OCA], [https://pdbe.org/4gde PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gde RCSB], [https://www.ebi.ac.uk/pdbsum/4gde PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gde ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q4W1X2_ASPFM Q4W1X2_ASPFM]		[https://www.uniprot.org/uniprot/Q4W1X2_ASPFM Q4W1X2_ASPFM]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	UDP-galactopyranose mutase (UGM) plays an essential role in galactofuranose biosynthesis in microorganisms by catalyzing the conversion of UDP-galactopyranose to UDP-galactofuranose. The enzyme has gained attention recently as a promising target for the design of new antifungal, antitrypanosomal, and antileishmanial agents. Here we report the first crystal structure of UGM complexed with its redox partner NAD(P)H. Kinetic protein crystallography was used to obtain structures of oxidized Aspergillus fumigatus UGM (AfUGM) complexed with NADPH and NADH, as well as reduced AfUGM after dissociation of NADP+. NAD(P)H binds with the nicotinamide near the FAD isoalloxazine and the ADP moiety extending toward the mobile 200s active site flap. The nicotinamide riboside binding site overlaps that of the substrate galactopyranose moiety, thus NADPH and substrate binding are mutually exclusive. On the other hand, the pockets for the adenine of NADPH and uracil of the substrate are distinct and separated by only 6 A, which raises the possibility of designing novel inhibitors that bind both sites. All twelve residues that contact NADP(H) are conserved among eukaryotic UGMs. Residues that form the AMP pocket are absent in bacterial UGMs, which suggests that eukaryotic and bacterial UGMs have different NADP(H) binding sites. The structures address the longstanding question of how UGM binds NAD(P)H and provide new opportunities for drug discovery.
-
-	Identification of the NAD(P)H Binding Site of Eukaryotic UDP-Galactopyranose Mutase.,Dhatwalia R, Singh H, Solano LM, Oppenheimer M, Robinson RM, Ellerbrock JF, Sobrado P, Tanner JJ J Am Chem Soc. 2012 Oct 4. PMID:23036087<ref>PMID:23036087</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4gde" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[UDP-galactopyranose mutase 3D structures\|UDP-galactopyranose mutase 3D structures]]		*[[UDP-galactopyranose mutase 3D structures\|UDP-galactopyranose mutase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:49, 26 October 2022

2022-10-26T06:49:10Z

←Older revision		Revision as of 06:49, 26 October 2022
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	<StructureSection load='4gde' size='340' side='right'caption='[[4gde]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4gde' size='340' side='right'caption='[[4gde]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gde]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aspfm Aspfm~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GDE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gde]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GDE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3utf\|3utf]], [[3utg\|3utg]], [[3uth\|3uth]], [[4gdc\|4gdc]], [[4gdd\|4gdd]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gde OCA], [https://pdbe.org/4gde PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gde RCSB], [https://www.ebi.ac.uk/pdbsum/4gde PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gde ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 ASPFM])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gde OCA], [~~http~~://pdbe.org/4gde PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gde RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gde PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gde ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q4W1X2_ASPFM Q4W1X2_ASPFM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
-	*[[UDP-galactopyranose mutase\|UDP-galactopyranose mutase]]	+	*[[UDP-galactopyranose mutase 3D structures\|UDP-galactopyranose mutase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspfm~~]]	+	[[Category: Aspergillus fumigatus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: UDP-galactopyranose mutase]]~~	+	[[Category: Dhatwalia RD]]
-	[[Category: Dhatwalia~~, R D~~]]	+	[[Category: Singh H]]
-	[[Category: Singh, H]]	+	[[Category: Tanner JJ]]
-	[[Category: Tanner~~, J J]]~~	+
-	~~[[Category: Flavin adenine dinucleotide binding]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Mutase]]~~	+
-	~~[[Category: Nucleotide binding~~]]	+

OCA at 07:50, 27 March 2019

2019-03-27T07:50:37Z

←Older revision		Revision as of 07:50, 27 March 2019
Line 1:		Line 1:

	==Crystal structure of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose==		==Crystal structure of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose==
-	<StructureSection load='4gde' size='340' side='right' caption='[[4gde]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='4gde' size='340' side='right'caption='[[4gde]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gde]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GDE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gde]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfm Aspfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GDE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3utf\|3utf]], [[3utg\|3utg]], [[3uth\|3uth]], [[4gdc\|4gdc]], [[4gdd\|4gdd]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3utf\|3utf]], [[3utg\|3utg]], [[3uth\|3uth]], [[4gdc\|4gdc]], [[4gdd\|4gdd]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 ASPFM])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gde OCA], [http://pdbe.org/4gde PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gde RCSB], [http://www.ebi.ac.uk/pdbsum/4gde PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gde ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gde OCA], [http://pdbe.org/4gde PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gde RCSB], [http://www.ebi.ac.uk/pdbsum/4gde PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gde ProSAT]</span></td></tr>
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspfm]]
		+	[[Category: Large Structures]]
	[[Category: UDP-galactopyranose mutase]]		[[Category: UDP-galactopyranose mutase]]
	[[Category: Dhatwalia, R D]]		[[Category: Dhatwalia, R D]]

OCA at 15:02, 22 December 2016

2016-12-22T15:02:38Z

←Older revision		Revision as of 15:02, 22 December 2016
Line 1:		Line 1:

-	==Crystal ~~strucure~~ of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose==	+	==Crystal structure of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose==
	<StructureSection load='4gde' size='340' side='right' caption='[[4gde]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4gde' size='340' side='right' caption='[[4gde]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gde]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Aspfm Aspfm]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GDE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gde]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GDE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3utf\|3utf]], [[3utg\|3utg]], [[3uth\|3uth]], [[4gdc\|4gdc]], [[4gdd\|4gdd]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3utf\|3utf]], [[3utg\|3utg]], [[3uth\|3uth]], [[4gdc\|4gdc]], [[4gdd\|4gdd]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 ASPFM])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gde OCA], [http://pdbe.org/4gde PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gde RCSB], [http://www.ebi.ac.uk/pdbsum/4gde PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gde ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gde OCA], [http://pdbe.org/4gde PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gde RCSB], [http://www.ebi.ac.uk/pdbsum/4gde PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gde ProSAT]</span></td></tr>
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aspfm]]
	[[Category: UDP-galactopyranose mutase]]		[[Category: UDP-galactopyranose mutase]]
	[[Category: Dhatwalia, R D]]		[[Category: Dhatwalia, R D]]

OCA at 20:08, 15 December 2016

2016-12-15T20:08:46Z

←Older revision		Revision as of 20:08, 15 December 2016
Line 1:		Line 1:
		+
	==Crystal strucure of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose==		==Crystal strucure of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose==
	<StructureSection load='4gde' size='340' side='right' caption='[[4gde]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4gde' size='340' side='right' caption='[[4gde]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gde]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aspergillus_fumigatus Aspergillus fumigatus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GDE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gde]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfm Aspfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GDE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3utf\|3utf]], [[3utg\|3utg]], [[3uth\|3uth]], [[4gdc\|4gdc]], [[4gdd\|4gdd]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3utf\|3utf]], [[3utg\|3utg]], [[3uth\|3uth]], [[4gdc\|4gdc]], [[4gdd\|4gdd]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 ~~Aspergillus fumigatus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 ASPFM])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gde OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gde RCSB], [http://www.ebi.ac.uk/pdbsum/4gde PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gde OCA], [http://pdbe.org/4gde PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gde RCSB], [http://www.ebi.ac.uk/pdbsum/4gde PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gde ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4gde" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspergillus fumigatus~~]]	+	[[Category: Aspfm]]
	[[Category: UDP-galactopyranose mutase]]		[[Category: UDP-galactopyranose mutase]]
	[[Category: Dhatwalia, R D]]		[[Category: Dhatwalia, R D]]

OCA at 09:18, 15 February 2015

2015-02-15T09:18:49Z

←Older revision		Revision as of 09:18, 15 February 2015
Line 1:		Line 1:
-	[[~~Image:~~4gde.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal strucure of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose==
		+	<StructureSection load='4gde' size='340' side='right' caption='[[4gde]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4gde]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GDE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3utf\|3utf]], [[3utg\|3utg]], [[3uth\|3uth]], [[4gdc\|4gdc]], [[4gdd\|4gdd]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 Aspergillus fumigatus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-galactopyranose_mutase UDP-galactopyranose mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.9 5.4.99.9] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gde OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gde RCSB], [http://www.ebi.ac.uk/pdbsum/4gde PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	UDP-galactopyranose mutase (UGM) plays an essential role in galactofuranose biosynthesis in microorganisms by catalyzing the conversion of UDP-galactopyranose to UDP-galactofuranose. The enzyme has gained attention recently as a promising target for the design of new antifungal, antitrypanosomal, and antileishmanial agents. Here we report the first crystal structure of UGM complexed with its redox partner NAD(P)H. Kinetic protein crystallography was used to obtain structures of oxidized Aspergillus fumigatus UGM (AfUGM) complexed with NADPH and NADH, as well as reduced AfUGM after dissociation of NADP+. NAD(P)H binds with the nicotinamide near the FAD isoalloxazine and the ADP moiety extending toward the mobile 200s active site flap. The nicotinamide riboside binding site overlaps that of the substrate galactopyranose moiety, thus NADPH and substrate binding are mutually exclusive. On the other hand, the pockets for the adenine of NADPH and uracil of the substrate are distinct and separated by only 6 A, which raises the possibility of designing novel inhibitors that bind both sites. All twelve residues that contact NADP(H) are conserved among eukaryotic UGMs. Residues that form the AMP pocket are absent in bacterial UGMs, which suggests that eukaryotic and bacterial UGMs have different NADP(H) binding sites. The structures address the longstanding question of how UGM binds NAD(P)H and provide new opportunities for drug discovery.

-	~~{{STRUCTURE_4gde\| PDB=4gde \| SCENE= }}~~	+	Identification of the NAD(P)H Binding Site of Eukaryotic UDP-Galactopyranose Mutase.,Dhatwalia R, Singh H, Solano LM, Oppenheimer M, Robinson RM, Ellerbrock JF, Sobrado P, Tanner JJ J Am Chem Soc. 2012 Oct 4. PMID:23036087<ref>PMID:23036087</ref>

-	~~===Crystal strucure~~ of ~~NADPH-reduced Aspergillus fumigatus UDP-galactopyranose===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	==~~About this Structure~~==	+	*[[UDP-galactopyranose mutase\|UDP-galactopyranose mutase]]
-	[[~~4gde~~]] ~~is a 4 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA].~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Aspergillus fumigatus]]		[[Category: Aspergillus fumigatus]]
	[[Category: UDP-galactopyranose mutase]]		[[Category: UDP-galactopyranose mutase]]
-	[[Category: Dhatwalia, R D.]]	+	[[Category: Dhatwalia, R D]]
-	[[Category: Singh, H.]]	+	[[Category: Singh, H]]
-	[[Category: Tanner, J J.]]	+	[[Category: Tanner, J J]]
	[[Category: Flavin adenine dinucleotide binding]]		[[Category: Flavin adenine dinucleotide binding]]
	[[Category: Isomerase]]		[[Category: Isomerase]]
	[[Category: Mutase]]		[[Category: Mutase]]
	[[Category: Nucleotide binding]]		[[Category: Nucleotide binding]]

OCA at 07:16, 14 November 2012

2012-11-14T07:16:24Z

←Older revision		Revision as of 07:16, 14 November 2012
Line 1:		Line 1:
-	[[Image:4gde.~~jpg~~\|left\|200px]]	+	[[Image:4gde.png\|left\|200px]]

	{{STRUCTURE_4gde\| PDB=4gde \| SCENE= }}		{{STRUCTURE_4gde\| PDB=4gde \| SCENE= }}
Line 5:		Line 5:
	===Crystal strucure of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose===		===Crystal strucure of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose===

-	{{ABSTRACT_PUBMED_23036087}}

	==About this Structure==		==About this Structure==

OCA at 07:06, 17 October 2012

2012-10-17T07:06:51Z

←Older revision		Revision as of 07:06, 17 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4gde.jpg\|left\|200px]]

-	~~The entry~~ 4gde ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4gde\| PDB=4gde \| SCENE= }}

-	~~Authors: Tanner, J.J., Dhatwalia, R.D., Singh, H.~~	+	===Crystal strucure of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose===

-	~~Description~~: ~~Crystal strucure of NADPH~~-~~reduced~~ Aspergillus fumigatus UDP-galactopyranose	+	{{ABSTRACT_PUBMED_23036087}}
		+
		+	==About this Structure==
		+	[[4gde]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GDE OCA].
		+	[[Category: Aspergillus fumigatus]]
		+	[[Category: UDP-galactopyranose mutase]]
		+	[[Category: Dhatwalia, R D.]]
		+	[[Category: Singh, H.]]
		+	[[Category: Tanner, J J.]]
		+	[[Category: Flavin adenine dinucleotide binding]]
		+	[[Category: Isomerase]]
		+	[[Category: Mutase]]
		+	[[Category: Nucleotide binding]]

OCA at 04:45, 29 August 2012

2012-08-29T04:45:16Z

←Older revision		Revision as of 04:45, 29 August 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4gde is ON HOLD	+	The entry 4gde is ON HOLD until Paper Publication

	Authors: Tanner, J.J., Dhatwalia, R.D., Singh, H.		Authors: Tanner, J.J., Dhatwalia, R.D., Singh, H.

	Description: Crystal strucure of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose		Description: Crystal strucure of NADPH-reduced Aspergillus fumigatus UDP-galactopyranose

OCA: Protected "4gde" [edit=sysop:move=sysop]

2012-08-08T05:01:15Z

Protected "4gde" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:01, 8 August 2012