4gou - Revision history

OCA at 07:08, 26 October 2022

2022-10-26T07:08:38Z

←Older revision		Revision as of 07:08, 26 October 2022
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	==Crystal structure of an RGS-RhoGEF from Entamoeba histolytica==		==Crystal structure of an RGS-RhoGEF from Entamoeba histolytica==
-	<StructureSection load='4gou' size='340' side='right' caption='[[4gou]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4gou' size='340' side='right'caption='[[4gou]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gou]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Enthi Enthi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GOU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gou]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GOU FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fid\|4fid]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gou OCA], [https://pdbe.org/4gou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gou RCSB], [https://www.ebi.ac.uk/pdbsum/4gou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gou ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EHI_010670, EhRGS-RhoGEF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gou OCA], [~~http~~://pdbe.org/4gou PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gou RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gou PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gou ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C4LYV4_ENTHI C4LYV4_ENTHI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enthi~~]]	+	[[Category: Entamoeba histolytica]]
-	[[Category: Bosch~~, D E~~]]	+	[[Category: Large Structures]]
-	[[Category: Kimple~~, A J~~]]	+	[[Category: Bosch DE]]
-	[[Category: Machius, M]]	+	[[Category: Kimple AJ]]
-	[[Category: Muller~~, R E~~]]	+	[[Category: Machius M]]
-	[[Category: Siderovski~~, D P~~]]	+	[[Category: Muller RE]]
-	[[Category: Willard~~, F S]]~~	+	[[Category: Siderovski DP]]
-	~~[[Category: Dh domain]]~~	+	[[Category: Willard FS]]
-	~~[[Category: Ehgalpha1]]~~	+
-	~~[[Category: Gtpase accelerating protein]]~~	+
-	~~[[Category: Heterotrimeric g protein effector]]~~	+
-	~~[[Category: Ph domain]]~~	+
-	~~[[Category: Phospholipid binding]]~~	+
-	~~[[Category: Rgs domain]]~~	+
-	~~[[Category: Rho guanine nucleotide exchange factor]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 20:02, 15 December 2016

2016-12-15T20:02:00Z

←Older revision		Revision as of 20:02, 15 December 2016
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		+
	==Crystal structure of an RGS-RhoGEF from Entamoeba histolytica==		==Crystal structure of an RGS-RhoGEF from Entamoeba histolytica==
	<StructureSection load='4gou' size='340' side='right' caption='[[4gou]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4gou' size='340' side='right' caption='[[4gou]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gou]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Entamoeba_histolytica Entamoeba histolytica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GOU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gou]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enthi Enthi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GOU FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fid\|4fid]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fid\|4fid]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EHI_010670, EhRGS-RhoGEF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ~~Entamoeba histolytica~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EHI_010670, EhRGS-RhoGEF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gou OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gou RCSB], [http://www.ebi.ac.uk/pdbsum/4gou PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gou OCA], [http://pdbe.org/4gou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gou RCSB], [http://www.ebi.ac.uk/pdbsum/4gou PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gou ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4gou" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Entamoeba histolytica~~]]	+	[[Category: Enthi]]
	[[Category: Bosch, D E]]		[[Category: Bosch, D E]]
	[[Category: Kimple, A J]]		[[Category: Kimple, A J]]

OCA at 09:39, 10 December 2014

2014-12-10T09:39:46Z

←Older revision		Revision as of 09:39, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4gou.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of an RGS-RhoGEF from Entamoeba histolytica==
		+	<StructureSection load='4gou' size='340' side='right' caption='[[4gou]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4gou]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GOU FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fid\|4fid]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EHI_010670, EhRGS-RhoGEF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 Entamoeba histolytica])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gou OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gou RCSB], [http://www.ebi.ac.uk/pdbsum/4gou PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	G protein signaling pathways, as key components of physiologic responsiveness and timing, are frequent targets for pharmacologic intervention. Here, we identify an effector for heterotrimeric G protein alpha subunit (EhGalpha1) signaling from Entamoeba histolytica, the causative agent of amoebic colitis. EhGalpha1 interacts with this effector and guanosine triphosphatase-accelerating protein, EhRGS-RhoGEF, in a nucleotide state-selective fashion. Coexpression of EhRGS-RhoGEF with constitutively active EhGalpha1 and EhRacC leads to Rac-dependent spreading in Drosophila S2 cells. EhRGS-RhoGEF overexpression in E. histolytica trophozoites leads to reduced migration toward serum and lower cysteine protease activity, as well as reduced attachment to, and killing of, host cells. A 2.3 A crystal structure of the full-length EhRGS-RhoGEF reveals a putative inhibitory helix engaging the Dbl homology domain Rho-binding surface and the pleckstrin homology domain. Mutational analysis of the EhGalpha1/EhRGS-RhoGEF interface confirms a canonical "regulator of G protein signaling" domain rather than a RhoGEF-RGS ("rgRGS") domain, suggesting a convergent evolution toward heterotrimeric and small G protein cross-talk.

-	~~{{STRUCTURE_4gou\| PDB=4gou \| SCENE= }}~~	+	Structural Determinants of RGS-RhoGEF Signaling Critical to Entamoeba histolytica Pathogenesis.,Bosch DE, Kimple AJ, Manning AJ, Muller RE, Willard FS, Machius M, Rogers SL, Siderovski DP Structure. 2012 Dec 19. pii: S0969-2126(12)00427-3. doi:, 10.1016/j.str.2012.11.012. PMID:23260656<ref>PMID:23260656</ref>

-	~~===Crystal structure~~ of ~~an RGS-RhoGEF from Entamoeba histolytica===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23260656}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4gou]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOU OCA].~~	+	</StructureSection>
	[[Category: Entamoeba histolytica]]		[[Category: Entamoeba histolytica]]
-	[[Category: Bosch, D E.]]	+	[[Category: Bosch, D E]]
-	[[Category: Kimple, A J.]]	+	[[Category: Kimple, A J]]
-	[[Category: Machius, M.]]	+	[[Category: Machius, M]]
-	[[Category: Muller, R E.]]	+	[[Category: Muller, R E]]
-	[[Category: Siderovski, D P.]]	+	[[Category: Siderovski, D P]]
-	[[Category: Willard, F S.]]	+	[[Category: Willard, F S]]
	[[Category: Dh domain]]		[[Category: Dh domain]]
	[[Category: Ehgalpha1]]		[[Category: Ehgalpha1]]

OCA at 12:50, 30 January 2013

2013-01-30T12:50:59Z

←Older revision		Revision as of 12:50, 30 January 2013
Line 1:		Line 1:
-	[[Image:4gou.~~jpg~~\|left\|200px]]	+	[[Image:4gou.png\|left\|200px]]

	{{STRUCTURE_4gou\| PDB=4gou \| SCENE= }}		{{STRUCTURE_4gou\| PDB=4gou \| SCENE= }}

OCA at 08:11, 9 January 2013

2013-01-09T08:11:07Z

←Older revision		Revision as of 08:11, 9 January 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4gou.jpg\|left\|200px]]

-	~~The entry~~ 4gou ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4gou\| PDB=4gou \| SCENE= }}

-	~~Authors: Bosch, D.E., Kimple, A.J., Muller, R.E., Willard, F.S., Machius, M., Siderovski, D.P.~~	+	===Crystal structure of an RGS-RhoGEF from Entamoeba histolytica===

-	~~Description: Crystal~~ structure ~~of an RGS-RhoGEF~~ from Entamoeba histolytica	+	{{ABSTRACT_PUBMED_23260656}}
		+
		+	==About this Structure==
		+	[[4gou]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOU OCA].
		+	[[Category: Entamoeba histolytica]]
		+	[[Category: Bosch, D E.]]
		+	[[Category: Kimple, A J.]]
		+	[[Category: Machius, M.]]
		+	[[Category: Muller, R E.]]
		+	[[Category: Siderovski, D P.]]
		+	[[Category: Willard, F S.]]
		+	[[Category: Dh domain]]
		+	[[Category: Ehgalpha1]]
		+	[[Category: Gtpase accelerating protein]]
		+	[[Category: Heterotrimeric g protein effector]]
		+	[[Category: Ph domain]]
		+	[[Category: Phospholipid binding]]
		+	[[Category: Rgs domain]]
		+	[[Category: Rho guanine nucleotide exchange factor]]
		+	[[Category: Signaling protein]]

OCA at 20:07, 26 September 2012

2012-09-26T20:07:39Z

←Older revision		Revision as of 20:07, 26 September 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4gou is ON HOLD	+	The entry 4gou is ON HOLD until Paper Publication

	Authors: Bosch, D.E., Kimple, A.J., Muller, R.E., Willard, F.S., Machius, M., Siderovski, D.P.		Authors: Bosch, D.E., Kimple, A.J., Muller, R.E., Willard, F.S., Machius, M., Siderovski, D.P.

	Description: Crystal structure of an RGS-RhoGEF from Entamoeba histolytica		Description: Crystal structure of an RGS-RhoGEF from Entamoeba histolytica

OCA: Protected "4gou" [edit=sysop:move=sysop]

2012-08-29T04:49:15Z

Protected "4gou" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:49, 29 August 2012

OCA: New page: '''Unreleased structure''' The entry 4gou is ON HOLD Authors: Bosch, D.E., Kimple, A.J., Muller, R.E., Willard, F.S., Machius, M., Siderovski, D.P. Description: Crystal structure of an...

2012-08-29T04:49:15Z

New page: '''Unreleased structure''' The entry 4gou is ON HOLD Authors: Bosch, D.E., Kimple, A.J., Muller, R.E., Willard, F.S., Machius, M., Siderovski, D.P. Description: Crystal structure of an...

New page

'''Unreleased structure'''

The entry 4gou is ON HOLD

Authors: Bosch, D.E., Kimple, A.J., Muller, R.E., Willard, F.S., Machius, M., Siderovski, D.P.

Description: Crystal structure of an RGS-RhoGEF from Entamoeba histolytica