4gt7 - Revision history

OCA at 07:16, 26 October 2022

2022-10-26T07:16:24Z

←Older revision		Revision as of 07:16, 26 October 2022
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	==An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation==		==An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation==
-	<StructureSection load='4gt7' size='340' side='right' caption='[[4gt7]], [[Resolution\|resolution]] 2.61Å' scene=''>	+	<StructureSection load='4gt7' size='340' side='right'caption='[[4gt7]], [[Resolution\|resolution]] 2.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gt7]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GT7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GT7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gt7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GT7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGHE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gt7 OCA], [https://pdbe.org/4gt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gt7 RCSB], [https://www.ebi.ac.uk/pdbsum/4gt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gt7 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gt7 OCA], [~~http~~://pdbe.org/4gt7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gt7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gt7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gt7 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IGHE_HUMAN IGHE_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Jardetzky, T S~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kim, B K~~]]	+	[[Category: Jardetzky TS]]
-	[[Category: ~~Wurzburg, B A~~]]	+	[[Category: Kim BK]]
-	[[Category: ~~Antibody]]~~	+	[[Category: Wurzburg BA]]
-	~~[[Category: Fc fragment]]~~	+
-	~~[[Category: Fc receptor]]~~	+
-	~~[[Category: Ige]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin~~]]	+

OCA at 19:59, 15 December 2016

2016-12-15T19:59:52Z

←Older revision		Revision as of 19:59, 15 December 2016
Line 1:		Line 1:
		+
	==An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation==		==An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation==
	<StructureSection load='4gt7' size='340' side='right' caption='[[4gt7]], [[Resolution\|resolution]] 2.61Å' scene=''>		<StructureSection load='4gt7' size='340' side='right' caption='[[4gt7]], [[Resolution\|resolution]] 2.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gt7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GT7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gt7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GT7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGHE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGHE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gt7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gt7 RCSB], [http://www.ebi.ac.uk/pdbsum/4gt7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gt7 OCA], [http://pdbe.org/4gt7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gt7 RCSB], [http://www.ebi.ac.uk/pdbsum/4gt7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gt7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4gt7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Jardetzky, T S]]		[[Category: Jardetzky, T S]]
	[[Category: Kim, B K]]		[[Category: Kim, B K]]

OCA at 09:35, 10 December 2014

2014-12-10T09:35:16Z

←Older revision		Revision as of 09:35, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4gt7.~~png~~\|~~left~~\|~~200px~~]]	+	==An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation==
		+	<StructureSection load='4gt7' size='340' side='right' caption='[[4gt7]], [[Resolution\|resolution]] 2.61Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4gt7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GT7 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGHE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gt7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gt7 RCSB], [http://www.ebi.ac.uk/pdbsum/4gt7 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	IgE antibodies interact with the high affinity IgE Fc receptor, FcepsilonRI, and activate inflammatory pathways associated with the allergic response. The IgE-Fc region, comprising the C-terminal domains of the IgE heavy chain, binds FcepsilonRI and can adopt different conformations ranging from a closed form incompatible with receptor binding to an open, receptor-bound state. A number of intermediate states are also observed in different IgE-Fc crystal forms. To further explore this apparent IgE-Fc conformational flexibility and to potentially trap a closed, inactive state, we generated a series of disulfide bond mutants. Here we describe the structure and biochemical properties of an IgE-Fc mutant that is trapped in the closed, non-receptor binding state via an engineered disulfide at residue 335 (Cys-335). Reduction of the disulfide at Cys-335 restores the ability of IgE-Fc to bind to its high affinity receptor, FcepsilonRIalpha. The structure of the Cys-335 mutant shows that its conformation is within the range of previously observed, closed form IgE-Fc structures and that it retains the hydrophobic pocket found in the hinge region of the closed conformation. Locking the IgE-Fc into the closed state with the Cys-335 mutation does not affect binding of two other IgE-Fc ligands, omalizumab and DARPin E2_79, demonstrating selective blocking of the high affinity receptor binding.

-	~~{{STRUCTURE_4gt7\| PDB=4gt7 \| SCENE= }}~~	+	An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, nonreceptor binding conformation.,Wurzburg BA, Kim B, Tarchevskaya SS, Eggel A, Vogel M, Jardetzky TS J Biol Chem. 2012 Oct 19;287(43):36251-7. doi: 10.1074/jbc.M112.407502. Epub 2012, Sep 4. PMID:22948141<ref>PMID:22948141</ref>

-	~~===An engineered disulfide bond reversibly traps~~ the ~~IgE-Fc3-4 in a closed, non-receptor binding conformation===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22948141}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4gt7]] is a 4 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GT7 OCA].~~	+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Jardetzky, T S.]]	+	[[Category: Jardetzky, T S]]
-	[[Category: Kim, B K.]]	+	[[Category: Kim, B K]]
-	[[Category: Wurzburg, B A.]]	+	[[Category: Wurzburg, B A]]
	[[Category: Antibody]]		[[Category: Antibody]]
	[[Category: Fc fragment]]		[[Category: Fc fragment]]

OCA at 07:52, 2 January 2013

2013-01-02T07:52:20Z

←Older revision		Revision as of 07:52, 2 January 2013
Line 1:		Line 1:
-	[[Image:4gt7.~~jpg~~\|left\|200px]]	+	[[Image:4gt7.png\|left\|200px]]

	{{STRUCTURE_4gt7\| PDB=4gt7 \| SCENE= }}		{{STRUCTURE_4gt7\| PDB=4gt7 \| SCENE= }}
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	===An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation===		===An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation===

		+	{{ABSTRACT_PUBMED_22948141}}

	==About this Structure==		==About this Structure==

OCA at 05:24, 12 September 2012

2012-09-12T05:24:13Z

←Older revision		Revision as of 05:24, 12 September 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4gt7.jpg\|left\|200px]]

-	~~The entry~~ 4gt7 ~~is ON HOLD~~	+	{{STRUCTURE_4gt7\| PDB=4gt7 \| SCENE= }}

-	~~Authors: Wurzburg~~, ~~B.A., Kim, B.K., Jardetzky, T.S.~~	+	===An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation===

-	~~Description~~: ~~An engineered disulfide bond reversibly traps the IgE~~-~~Fc3-4 in a closed~~, ~~non-~~receptor ~~binding conformation~~	+
		+	==About this Structure==
		+	[[4gt7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GT7 OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Jardetzky, T S.]]
		+	[[Category: Kim, B K.]]
		+	[[Category: Wurzburg, B A.]]
		+	[[Category: Antibody]]
		+	[[Category: Fc fragment]]
		+	[[Category: Fc receptor]]
		+	[[Category: Ige]]
		+	[[Category: Immune system]]
		+	[[Category: Immunoglobulin]]

OCA: Protected "4gt7" [edit=sysop:move=sysop]

2012-09-05T04:58:29Z

Protected "4gt7" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:58, 5 September 2012

OCA: New page: '''Unreleased structure''' The entry 4gt7 is ON HOLD Authors: Wurzburg, B.A., Kim, B.K., Jardetzky, T.S. Description: An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a c...

2012-09-05T04:58:28Z

New page: '''Unreleased structure''' The entry 4gt7 is ON HOLD Authors: Wurzburg, B.A., Kim, B.K., Jardetzky, T.S. Description: An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a c...

New page

'''Unreleased structure'''

The entry 4gt7 is ON HOLD

Authors: Wurzburg, B.A., Kim, B.K., Jardetzky, T.S.

Description: An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation