4gtw - Revision history

OCA at 07:17, 26 October 2022

2022-10-26T07:17:40Z

←Older revision		Revision as of 07:17, 26 October 2022
Line 1:		Line 1:

	==Crystal structure of mouse Enpp1 in complex with AMP==		==Crystal structure of mouse Enpp1 in complex with AMP==
-	<StructureSection load='4gtw' size='340' side='right' caption='[[4gtw]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='4gtw' size='340' side='right'caption='[[4gtw]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gtw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GTW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GTW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gtw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GTW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gtw OCA], [https://pdbe.org/4gtw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gtw RCSB], [https://www.ebi.ac.uk/pdbsum/4gtw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gtw ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gtx\|4gtx]], [[4gty\|4gty]], [[4gtz\|4gtz]]</td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Enpp2, Npps2, Pdnp2, Enpp1, mCG_9001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkylglycerophosphoethanolamine_phosphodiesterase Alkylglycerophosphoethanolamine phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.39 3.1.4.39] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gtw OCA], [~~http~~://pdbe.org/4gtw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gtw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gtw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gtw ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/ENPP2_MOUSE ENPP2_MOUSE]] Note=May contribute to obesity.	+	[https://www.uniprot.org/uniprot/ENPP1_MOUSE ENPP1_MOUSE] Defects in Enpp1 are the cause of the tiptoe walking (ttw) phenotype. Ttw mice exhibit ossification of the spinal ligaments.<ref>PMID:9662402</ref> [https://www.uniprot.org/uniprot/ENPP2_MOUSE ENPP2_MOUSE] Note=May contribute to obesity.
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ENPP2_MOUSE ENPP2_MOUSE]] Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.<ref>PMID:15700135</ref> <ref>PMID:17208043</ref> <ref>PMID:21240269</ref>	+	[https://www.uniprot.org/uniprot/ENPP1_MOUSE ENPP1_MOUSE] Appears to modulate insulin sensitivity (By similarity). By generating PPi, plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. PPi inhibits mineralization by binding to nascent hydroxyapatite (HA) crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling.<ref>PMID:1647027</ref> <ref>PMID:9662402</ref> [https://www.uniprot.org/uniprot/ENPP2_MOUSE ENPP2_MOUSE] Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.<ref>PMID:15700135</ref> <ref>PMID:17208043</ref> <ref>PMID:21240269</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Ectonucleotide pyrophosphatase/phosphodiesterase\|Ectonucleotide pyrophosphatase/phosphodiesterase]]		*[[Ectonucleotide pyrophosphatase/phosphodiesterase\|Ectonucleotide pyrophosphatase/phosphodiesterase]]
		+	*[[Ectonucleotide pyrophosphatase/phosphodiesterase 3D structures\|Ectonucleotide pyrophosphatase/phosphodiesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Alkylglycerophosphoethanolamine phosphodiesterase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Ishitani, R]]	+	[[Category: Ishitani R]]
-	[[Category: Kato, K]]	+	[[Category: Kato K]]
-	[[Category: Nishimasu, H]]	+	[[Category: Nishimasu H]]
-	[[Category: Nureki, O]]	+	[[Category: Nureki O]]
-	~~[[Category: Bone mineralization]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phosphodiesterase~~]]	+

OCA at 06:09, 20 June 2018

2018-06-20T06:09:48Z

←Older revision		Revision as of 06:09, 20 June 2018
Line 24:		Line 24:
	</div>		</div>
	<div class="pdbe-citations 4gtw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gtw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ectonucleotide pyrophosphatase/phosphodiesterase\|Ectonucleotide pyrophosphatase/phosphodiesterase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:32, 15 November 2017

2017-11-15T08:32:39Z

←Older revision		Revision as of 08:32, 15 November 2017
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gtw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GTW FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gtw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GTW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=~~NAG~~:~~N-ACETYL~~-D-~~GLUCOSAMINE~~'>~~NAG~~</scene>, <scene name='pdbligand=ZN:~~ZINC+ION~~'>ZN</scene>, <scene name='pdbligand=~~MAN~~:~~ALPHA-D-MANNOSE~~'>~~MAN~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gtx\|4gtx]], [[4gty\|4gty]], [[4gtz\|4gtz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gtx\|4gtx]], [[4gty\|4gty]], [[4gtz\|4gtz]]</td></tr>

OCA at 20:03, 15 December 2016

2016-12-15T20:03:40Z

←Older revision		Revision as of 20:03, 15 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of mouse Enpp1 in complex with AMP==		==Crystal structure of mouse Enpp1 in complex with AMP==
	<StructureSection load='4gtw' size='340' side='right' caption='[[4gtw]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4gtw' size='340' side='right' caption='[[4gtw]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gtw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GTW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gtw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GTW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gtx\|4gtx]], [[4gty\|4gty]], [[4gtz\|4gtz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gtx\|4gtx]], [[4gty\|4gty]], [[4gtz\|4gtz]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Enpp2, Npps2, Pdnp2, Enpp1, mCG_9001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Enpp2, Npps2, Pdnp2, Enpp1, mCG_9001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkylglycerophosphoethanolamine_phosphodiesterase Alkylglycerophosphoethanolamine phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.39 3.1.4.39] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkylglycerophosphoethanolamine_phosphodiesterase Alkylglycerophosphoethanolamine phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.39 3.1.4.39] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gtw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gtw RCSB], [http://www.ebi.ac.uk/pdbsum/4gtw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gtw OCA], [http://pdbe.org/4gtw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gtw RCSB], [http://www.ebi.ac.uk/pdbsum/4gtw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gtw ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 22:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4gtw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Alkylglycerophosphoethanolamine phosphodiesterase]]		[[Category: Alkylglycerophosphoethanolamine phosphodiesterase]]
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Ishitani, R]]		[[Category: Ishitani, R]]
	[[Category: Kato, K]]		[[Category: Kato, K]]

OCA at 09:43, 10 December 2014

2014-12-10T09:43:17Z

←Older revision		Revision as of 09:43, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4gtw.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of mouse Enpp1 in complex with AMP==
		+	<StructureSection load='4gtw' size='340' side='right' caption='[[4gtw]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4gtw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GTW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gtx\|4gtx]], [[4gty\|4gty]], [[4gtz\|4gtz]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Enpp2, Npps2, Pdnp2, Enpp1, mCG_9001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkylglycerophosphoethanolamine_phosphodiesterase Alkylglycerophosphoethanolamine phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.39 3.1.4.39] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gtw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gtw RCSB], [http://www.ebi.ac.uk/pdbsum/4gtw PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/ENPP2_MOUSE ENPP2_MOUSE]] Note=May contribute to obesity.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ENPP2_MOUSE ENPP2_MOUSE]] Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.<ref>PMID:15700135</ref> <ref>PMID:17208043</ref> <ref>PMID:21240269</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enpp1 is a membrane-bound glycoprotein that regulates bone mineralization by hydrolyzing extracellular nucleotide triphosphates to produce pyrophosphate. Enpp1 dysfunction causes human diseases characterized by ectopic calcification. Enpp1 also inhibits insulin signaling, and an Enpp1 polymorphism is associated with insulin resistance. However, the precise mechanism by which Enpp1 functions in these cellular processes remains elusive. Here, we report the crystal structures of the extracellular region of mouse Enpp1 in complex with four different nucleotide monophosphates, at resolutions of 2.7-3.2 A. The nucleotides are accommodated in a pocket formed by an insertion loop in the catalytic domain, explaining the preference of Enpp1 for an ATP substrate. Structural mapping of disease-associated mutations indicated the functional importance of the interdomain interactions. A structural comparison of Enpp1 with Enpp2, a lysophospholipase D, revealed marked differences in the domain arrangements and active-site architectures. Notably, the Enpp1 mutant lacking the insertion loop lost the nucleotide-hydrolyzing activity but instead gained the lysophospholipid-hydrolyzing activity of Enpp2. Our findings provide structural insights into how the Enpp family proteins evolved to exert their diverse cellular functions.

-	~~{{STRUCTURE_4gtw\| PDB=4gtw \| SCENE= }}~~	+	Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signaling.,Kato K, Nishimasu H, Okudaira S, Mihara E, Ishitani R, Takagi J, Aoki J, Nureki O Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16876-81. doi:, 10.1073/pnas.1208017109. Epub 2012 Oct 1. PMID:23027977<ref>PMID:23027977</ref>

-	~~===Crystal structure~~ of ~~mouse Enpp1 in complex with AMP===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	==~~About this Structure~~==	+	<references/>
-	~~[[4gtw]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GTW OCA].~~	+	__TOC__
		+	</StructureSection>
	[[Category: Alkylglycerophosphoethanolamine phosphodiesterase]]		[[Category: Alkylglycerophosphoethanolamine phosphodiesterase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Ishitani, R.]]	+	[[Category: Ishitani, R]]
-	[[Category: Kato, K.]]	+	[[Category: Kato, K]]
-	[[Category: Nishimasu, H.]]	+	[[Category: Nishimasu, H]]
-	[[Category: Nureki, O.]]	+	[[Category: Nureki, O]]
	[[Category: Bone mineralization]]		[[Category: Bone mineralization]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Phosphodiesterase]]		[[Category: Phosphodiesterase]]

OCA at 13:48, 8 November 2012

2012-11-08T13:48:17Z

←Older revision		Revision as of 13:48, 8 November 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4gtw.jpg\|left\|200px]]

-	~~The entry~~ 4gtw ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4gtw\| PDB=4gtw \| SCENE= }}

-	~~Authors: Kato, K., Nishimasu, H., Ishitani, R., Nureki, O.~~	+	===Crystal structure of mouse Enpp1 in complex with AMP===

-	~~Description: Crystal~~ structure ~~of mouse Enpp1 in complex~~ with ~~AMP~~	+
		+	==About this Structure==
		+	[[4gtw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GTW OCA].
		+	[[Category: Alkylglycerophosphoethanolamine phosphodiesterase]]
		+	[[Category: Mus musculus]]
		+	[[Category: Ishitani, R.]]
		+	[[Category: Kato, K.]]
		+	[[Category: Nishimasu, H.]]
		+	[[Category: Nureki, O.]]
		+	[[Category: Bone mineralization]]
		+	[[Category: Hydrolase]]
		+	[[Category: Phosphodiesterase]]

OCA at 06:53, 10 October 2012

2012-10-10T06:53:54Z

←Older revision		Revision as of 06:53, 10 October 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4gtw is ON HOLD	+	The entry 4gtw is ON HOLD until Paper Publication

	Authors: Kato, K., Nishimasu, H., Ishitani, R., Nureki, O.		Authors: Kato, K., Nishimasu, H., Ishitani, R., Nureki, O.

	Description: Crystal structure of mouse Enpp1 in complex with AMP		Description: Crystal structure of mouse Enpp1 in complex with AMP

OCA at 04:53, 12 September 2012

2012-09-12T04:53:28Z

←Older revision		Revision as of 04:53, 12 September 2012
Line 3:		Line 3:
	The entry 4gtw is ON HOLD		The entry 4gtw is ON HOLD

-	Authors: Kato K., Nishimasu, H., Ishitani, R., Nureki, O.	+	Authors: Kato, K., Nishimasu, H., Ishitani, R., Nureki, O.

	Description: Crystal structure of mouse Enpp1 in complex with AMP		Description: Crystal structure of mouse Enpp1 in complex with AMP

OCA: Protected "4gtw" [edit=sysop:move=sysop]

2012-09-05T04:59:11Z

Protected "4gtw" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:59, 5 September 2012

OCA: New page: '''Unreleased structure''' The entry 4gtw is ON HOLD Authors: Kato K., Nishimasu, H., Ishitani, R., Nureki, O. Description: Crystal structure of mouse Enpp1 in complex with AMP

2012-09-05T04:59:10Z

New page: '''Unreleased structure''' The entry 4gtw is ON HOLD Authors: Kato K., Nishimasu, H., Ishitani, R., Nureki, O. Description: Crystal structure of mouse Enpp1 in complex with AMP

New page

'''Unreleased structure'''

The entry 4gtw is ON HOLD

Authors: Kato K., Nishimasu, H., Ishitani, R., Nureki, O.

Description: Crystal structure of mouse Enpp1 in complex with AMP