4gv6 - Revision history

OCA at 11:34, 1 March 2024

2024-03-01T11:34:25Z

←Older revision		Revision as of 11:34, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gv6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lassa_virus_Josiah Lassa virus Josiah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GV6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gv6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lassa_virus_Josiah Lassa virus Josiah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GV6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.98Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gv6 OCA], [https://pdbe.org/4gv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gv6 RCSB], [https://www.ebi.ac.uk/pdbsum/4gv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gv6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gv6 OCA], [https://pdbe.org/4gv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gv6 RCSB], [https://www.ebi.ac.uk/pdbsum/4gv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gv6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/NCAP_LASSJ NCAP_LASSJ] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFN-beta) production. Strongly inhibits the nuclear translocation of IRF3, a protein involved in IFN activation pathway. Also inhibits the activation of IFN-beta and IRF3-dependent promoters (By similarity).		[https://www.uniprot.org/uniprot/NCAP_LASSJ NCAP_LASSJ] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFN-beta) production. Strongly inhibits the nuclear translocation of IRF3, a protein involved in IFN activation pathway. Also inhibits the activation of IFN-beta and IRF3-dependent promoters (By similarity).
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A hallmark of severe Lassa fever is the generalized immune suppression, the mechanism of which is poorly understood. Lassa virus (LASV) nucleoprotein (NP) is the only known 3-5 exoribonuclease that can suppress type I interferon (IFN) production possibly by degrading immune-stimulatory RNAs. How this unique enzymatic activity of LASV NP recognizes and processes RNA substrates is unknown. We provide an atomic view of a catalytically active exoribonuclease domain of LASV NP (LASV NP-C) in the process of degrading a 5 triphosphate double-stranded (ds) RNA substrate - a typical pathogen-associated molecular pattern (PAMP) molecule - to induce type I IFN production. Additionally, we provide for the first time a high-resolution crystal structure of an active exoribonuclease domain of Tacaribe arenavirus (TCRV) NP. Coupled with the in vitro enzymatic and cell-based interferon suppression assays, these structural analyses strongly support a unified model of an exoribonuclease-dependent IFN suppression mechanism shared by all known arenaviruses. New knowledge learned from these studies should aid the development of therapeutics against pathogenic arenaviruses that can infect hundreds of thousand individuals and kill thousands annually.
-
-	Structures of arenaviral nucleoproteins with triphosphate dsRNA reveal a unique mechanism of immune suppression.,Jiang X, Huang Q, Wang W, Dong H, Ly H, Liang Y, Dong C J Biol Chem. 2013 Apr 24. PMID:23615902<ref>PMID:23615902</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4gv6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]		*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:19, 26 October 2022

2022-10-26T07:19:49Z

←Older revision		Revision as of 07:19, 26 October 2022
Line 1:		Line 1:

	==Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production==		==Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production==
-	<StructureSection load='4gv6' size='340' side='right' caption='[[4gv6]], [[Resolution\|resolution]] 1.98Å' scene=''>	+	<StructureSection load='4gv6' size='340' side='right'caption='[[4gv6]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gv6]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lassj Lassj~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GV6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GV6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gv6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lassa_virus_Josiah Lassa virus Josiah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GV6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:~~MANGANESE+(II)+ION~~'>MN</scene>, <scene name='pdbligand=ZN:~~ZINC~~+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~GTP~~:~~GUANOSINE-5-TRIPHOSPHATE~~'>~~GTP~~</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gv6 OCA], [https://pdbe.org/4gv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gv6 RCSB], [https://www.ebi.ac.uk/pdbsum/4gv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gv6 ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mwp\|3mwp]], [[4g9z\|4g9z]], [[4gv3\|4gv3]]</td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11622 LASSJ])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gv6 OCA], [~~http~~://pdbe.org/4gv6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gv6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gv6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gv6 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NCAP_LASSJ NCAP_LASSJ]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFN-beta) production. Strongly inhibits the nuclear translocation of IRF3, a protein involved in IFN activation pathway. Also inhibits the activation of IFN-beta and IRF3-dependent promoters (By similarity).	+	[https://www.uniprot.org/uniprot/NCAP_LASSJ NCAP_LASSJ] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFN-beta) production. Strongly inhibits the nuclear translocation of IRF3, a protein involved in IFN activation pathway. Also inhibits the activation of IFN-beta and IRF3-dependent promoters (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 20:

	==See Also==		==See Also==
-	*[[Nucleoprotein\|Nucleoprotein]]	+	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lassj~~]]	+	[[Category: Large Structures]]
-	[[Category: Dong, C]]	+	[[Category: Lassa virus Josiah]]
-	[[Category: Dong, H]]	+	[[Category: Dong C]]
-	[[Category: Huang, Q]]	+	[[Category: Dong H]]
-	[[Category: Jiang, X]]	+	[[Category: Huang Q]]
-	[[Category: Liang, Y]]	+	[[Category: Jiang X]]
-	[[Category: Ly, H]]	+	[[Category: Liang Y]]
-	[[Category: Wang, W]]	+	[[Category: Ly H]]
-	~~[[Category: 3'-5' exonuclease]]~~	+	[[Category: Wang W]]
-	~~[[Category: Deddh family enzyme]]~~	+
-	~~[[Category: Rna binding protein-rna complex~~]]	+

OCA at 10:41, 4 August 2016

2016-08-04T10:41:00Z

←Older revision		Revision as of 10:41, 4 August 2016
Line 1:		Line 1:
		+
	==Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production==		==Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production==
	<StructureSection load='4gv6' size='340' side='right' caption='[[4gv6]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='4gv6' size='340' side='right' caption='[[4gv6]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gv6]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lassa_virus_josiah Lassa virus josiah~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GV6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gv6]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lassj Lassj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GV6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mwp\|3mwp]], [[4g9z\|4g9z]], [[4gv3\|4gv3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mwp\|3mwp]], [[4g9z\|4g9z]], [[4gv3\|4gv3]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11622 ~~Lassa virus Josiah~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11622 LASSJ])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gv6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gv6 RCSB], [http://www.ebi.ac.uk/pdbsum/4gv6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gv6 OCA], [http://pdbe.org/4gv6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gv6 RCSB], [http://www.ebi.ac.uk/pdbsum/4gv6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gv6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4gv6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lassa virus josiah~~]]	+	[[Category: Lassj]]
	[[Category: Dong, C]]		[[Category: Dong, C]]
	[[Category: Dong, H]]		[[Category: Dong, H]]

OCA at 08:27, 25 December 2014

2014-12-25T08:27:08Z

←Older revision		Revision as of 08:27, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gv6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gv6 RCSB], [http://www.ebi.ac.uk/pdbsum/4gv6 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gv6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gv6 RCSB], [http://www.ebi.ac.uk/pdbsum/4gv6 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NCAP_LASSJ NCAP_LASSJ]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFN-beta) production. Strongly inhibits the nuclear translocation of IRF3, a protein involved in IFN activation pathway. Also inhibits the activation of IFN-beta and IRF3-dependent promoters (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:41, 21 December 2014

2014-12-21T11:41:40Z

←Older revision		Revision as of 11:41, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4gv6\| PDB=4gv6 \| SCENE= }}~~	+	==Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production==
-	===Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production===	+	<StructureSection load='4gv6' size='340' side='right' caption='[[4gv6]], [[Resolution\|resolution]] 1.98Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23615902}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4gv6]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lassa_virus_josiah Lassa virus josiah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GV6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mwp\|3mwp]], [[4g9z\|4g9z]], [[4gv3\|4gv3]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11622 Lassa virus Josiah])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gv6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gv6 RCSB], [http://www.ebi.ac.uk/pdbsum/4gv6 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A hallmark of severe Lassa fever is the generalized immune suppression, the mechanism of which is poorly understood. Lassa virus (LASV) nucleoprotein (NP) is the only known 3-5 exoribonuclease that can suppress type I interferon (IFN) production possibly by degrading immune-stimulatory RNAs. How this unique enzymatic activity of LASV NP recognizes and processes RNA substrates is unknown. We provide an atomic view of a catalytically active exoribonuclease domain of LASV NP (LASV NP-C) in the process of degrading a 5 triphosphate double-stranded (ds) RNA substrate - a typical pathogen-associated molecular pattern (PAMP) molecule - to induce type I IFN production. Additionally, we provide for the first time a high-resolution crystal structure of an active exoribonuclease domain of Tacaribe arenavirus (TCRV) NP. Coupled with the in vitro enzymatic and cell-based interferon suppression assays, these structural analyses strongly support a unified model of an exoribonuclease-dependent IFN suppression mechanism shared by all known arenaviruses. New knowledge learned from these studies should aid the development of therapeutics against pathogenic arenaviruses that can infect hundreds of thousand individuals and kill thousands annually.

-	~~==Function==~~	+	Structures of arenaviral nucleoproteins with triphosphate dsRNA reveal a unique mechanism of immune suppression.,Jiang X, Huang Q, Wang W, Dong H, Ly H, Liang Y, Dong C J Biol Chem. 2013 Apr 24. PMID:23615902<ref>PMID:23615902</ref>
-	[[http://www.uniprot.org/uniprot/NCAP_LASSJ NCAP_LASSJ]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of ~~protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering~~ with ~~beta interferon (IFN-beta) production~~. ~~Strongly inhibits the nuclear translocation of IRF3~~, ~~a protein involved in IFN activation pathway~~. ~~Also inhibits the activation of IFN-beta and IRF3-dependent promoters (By similarity)~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4gv6]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lassa_virus_josiah Lassa virus josiah]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4GV6 OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:023615902</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Nucleoprotein\|Nucleoprotein]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Lassa virus josiah]]		[[Category: Lassa virus josiah]]
-	[[Category: Dong, C.]]	+	[[Category: Dong, C]]
-	[[Category: Dong, H.]]	+	[[Category: Dong, H]]
-	[[Category: Huang, Q.]]	+	[[Category: Huang, Q]]
-	[[Category: Jiang, X.]]	+	[[Category: Jiang, X]]
-	[[Category: Liang, Y.]]	+	[[Category: Liang, Y]]
-	[[Category: Ly, H.]]	+	[[Category: Ly, H]]
-	[[Category: Wang, W.]]	+	[[Category: Wang, W]]
	[[Category: 3'-5' exonuclease]]		[[Category: 3'-5' exonuclease]]
	[[Category: Deddh family enzyme]]		[[Category: Deddh family enzyme]]
	[[Category: Rna binding protein-rna complex]]		[[Category: Rna binding protein-rna complex]]

OCA at 14:24, 10 July 2013

2013-07-10T14:24:29Z

←Older revision		Revision as of 14:24, 10 July 2013
Line 8:		Line 8:
	==About this Structure==		==About this Structure==
	[[4gv6]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lassa_virus_josiah Lassa virus josiah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GV6 OCA].		[[4gv6]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lassa_virus_josiah Lassa virus josiah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GV6 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023615902</ref><references group="xtra"/><references/>
	[[Category: Lassa virus josiah]]		[[Category: Lassa virus josiah]]
	[[Category: Dong, C.]]		[[Category: Dong, C.]]

OCA at 06:14, 16 May 2013

2013-05-16T06:14:42Z

←Older revision		Revision as of 06:14, 16 May 2013
Line 1:		Line 1:
	{{STRUCTURE_4gv6\| PDB=4gv6 \| SCENE= }}		{{STRUCTURE_4gv6\| PDB=4gv6 \| SCENE= }}
	===Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production===		===Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production===
		+	{{ABSTRACT_PUBMED_23615902}}

	==Function==		==Function==

OCA at 07:50, 2 May 2013

2013-05-02T07:50:05Z

←Older revision		Revision as of 07:50, 2 May 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4gv6\| PDB=4gv6 \| SCENE= }}
		+	===Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production===

-	The ~~entry 4gv6~~ is ~~ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/NCAP_LASSJ NCAP_LASSJ]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFN-beta) production. Strongly inhibits the nuclear translocation of IRF3, a protein involved in IFN activation pathway. Also inhibits the activation of IFN-beta and IRF3-dependent promoters (By similarity).

-	~~Authors~~: ~~Jiang, X~~.~~, Huang, Q~~.~~, Wang, W~~., Dong, H., ~~Ly, H~~., Liang, Y., ~~Dong~~, C.	+	==About this Structure==
-		+	[[4gv6]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lassa_virus_josiah Lassa virus josiah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GV6 OCA].
-	~~Description~~: ~~Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique~~ 3 -5 ~~exoribonuclease mechanism to suppress type I interferon production~~	+	[[Category: Lassa virus josiah]]
		+	[[Category: Dong, C.]]
		+	[[Category: Dong, H.]]
		+	[[Category: Huang, Q.]]
		+	[[Category: Jiang, X.]]
		+	[[Category: Liang, Y.]]
		+	[[Category: Ly, H.]]
		+	[[Category: Wang, W.]]
		+	[[Category: 3'-5' exonuclease]]
		+	[[Category: Deddh family enzyme]]
		+	[[Category: Rna binding protein-rna complex]]

OCA at 13:18, 20 February 2013

2013-02-20T13:18:44Z

←Older revision		Revision as of 13:18, 20 February 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4gv6 is ON HOLD ~~until sometime in the future~~	+	The entry 4gv6 is ON HOLD

-	Authors: ~~Xue~~ Jiang, ~~Qingfeng~~ Huang, ~~Wenjian~~ Wang, ~~Haohao~~ Dong, ~~Hinh~~ Ly, ~~Yuying~~ Liang, ~~Changjiang~~ Dong	+	Authors: Jiang, X., Huang, Q., Wang, W., Dong, H., Ly, H., Liang, Y., Dong, C.

	Description: Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production		Description: Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production

OCA at 06:54, 19 September 2012

2012-09-19T06:54:41Z

←Older revision		Revision as of 06:54, 19 September 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4gv6 is ON HOLD	+	The entry 4gv6 is ON HOLD until sometime in the future

	Authors: Xue Jiang, Qingfeng Huang, Wenjian Wang, Haohao Dong, Hinh Ly, Yuying Liang, Changjiang Dong		Authors: Xue Jiang, Qingfeng Huang, Wenjian Wang, Haohao Dong, Hinh Ly, Yuying Liang, Changjiang Dong

	Description: Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production		Description: Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production