4gzb - Revision history

OCA at 14:03, 8 November 2023

2023-11-08T14:03:17Z

←Older revision		Revision as of 14:03, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gzb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GZB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gzb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GZB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.79Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzb OCA], [https://pdbe.org/4gzb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gzb RCSB], [https://www.ebi.ac.uk/pdbsum/4gzb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzb OCA], [https://pdbe.org/4gzb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gzb RCSB], [https://www.ebi.ac.uk/pdbsum/4gzb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzb ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:49, 3 November 2022

2022-11-03T07:49:18Z

←Older revision		Revision as of 07:49, 3 November 2022
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	==Crystal structure of native AmpC beta-lactamase from Pseudomonas aeruginosa PAO1==		==Crystal structure of native AmpC beta-lactamase from Pseudomonas aeruginosa PAO1==
-	<StructureSection load='4gzb' size='340' side='right' caption='[[4gzb]], [[Resolution\|resolution]] 1.79Å' scene=''>	+	<StructureSection load='4gzb' size='340' side='right'caption='[[4gzb]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gzb]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GZB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gzb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GZB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hbt\|4hbt]], [[4hbu\|4hbu]], [[4hef\|4hef]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzb OCA], [https://pdbe.org/4gzb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gzb RCSB], [https://www.ebi.ac.uk/pdbsum/4gzb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzb ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ampC, blaAmpC, PA4110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzb OCA], [~~http~~://pdbe.org/4gzb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gzb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gzb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzb ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AMPC_PSEAE AMPC_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
-	*[[Beta-lactamase\|Beta-lactamase]]	+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Beta-lactamase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Benvenuti, M]]	+	[[Category: Benvenuti M]]
-	[[Category: ~~Docquier, J D~~]]	+	[[Category: De Luca F]]
-	[[Category: ~~Luca, F De~~]]	+	[[Category: Docquier JD]]
-	[[Category: Mangani, S]]	+	[[Category: Mangani S]]
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Beta-lactamase fold]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 11:35, 4 August 2016

2016-08-04T11:35:20Z

←Older revision		Revision as of 11:35, 4 August 2016
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-	{{STRUCTURE_4gzb\| PDB=4gzb \| SCENE= }}
-	===Crystal structure of native AmpC beta-lactamase from Pseudomonas aeruginosa PAO1===
-	{{ABSTRACT_PUBMED_23439634}}

-	==~~About this Structure~~==	+	==Crystal structure of native AmpC beta-lactamase from Pseudomonas aeruginosa PAO1==
-	[[4gzb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZB OCA].	+	<StructureSection load='4gzb' size='340' side='right' caption='[[4gzb]], [[Resolution\|resolution]] 1.79Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4gzb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GZB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hbt\|4hbt]], [[4hbu\|4hbu]], [[4hef\|4hef]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ampC, blaAmpC, PA4110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzb OCA], [http://pdbe.org/4gzb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gzb RCSB], [http://www.ebi.ac.uk/pdbsum/4gzb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzb ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Although beta-lactams have been the most effective class of antibacterial agents used in clinical practice for the past half century, their effectiveness on Gram-negative bacteria has been eroded due to the emergence and spread of beta-lactamase enzymes that are not affected by currently marketed beta-lactam/beta-lactamase inhibitor combinations. Avibactam is a novel, covalent, non-beta-lactam beta-lactamase inhibitor presently in clinical development in combination with either ceftaroline or ceftazidime. In vitro studies show that avibactam may restore the broad-spectrum activity of cephalosporins against class A, class C and some class D beta-lactamases. Here we describe the structure of two clinically important beta-lactamase enzymes bound to avibactam, the class A CTX-M-15 extended-spectrum beta-lactamase and class C Pseudomonas aeruginosa AmpC beta-lactamase, which together provide insight into the binding modes for the respective enzyme classes. The structure reveals a similar binding mode in both enzymes and thus provides a rationale for the broad-spectrum inhibitory activity of avibactam. Identification of the key residues surrounding the binding pocket allows for a better understanding of the potency of this scaffold. Finally, avibactam has recently been shown to be a reversible inhibitor and the structure provides insights into the mechanism of avibactam recyclization. Analysis of the ultra-high resolution CTX-M-15 structure suggests how the deacylation mechanism favors recyclization over hydrolysis.
		+
		+	Structural Insight into Potent Broad-spectrum Inhibition with Reversible Recyclization Mechanism: Avibactam in Complex with CTX-M-15 and Pseudomonas aeruginosa AmpC beta-lactamases.,Lahiri SD, Mangani S, Durand-Reville T, Benvenuti M, De Luca F, Sanyal G, Docquier JD Antimicrob Agents Chemother. 2013 Feb 25. PMID:23439634<ref>PMID:23439634</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4gzb" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Beta-lactamase\|Beta-lactamase]]		*[[Beta-lactamase\|Beta-lactamase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023439634</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Pseae]]		[[Category: Pseae]]
-	[[Category: Benvenuti, M.]]	+	[[Category: Benvenuti, M]]
-	[[Category: Docquier, J D.]]	+	[[Category: Docquier, J D]]
-	[[Category: Luca, F De.]]	+	[[Category: Luca, F De]]
-	[[Category: Mangani, S.]]	+	[[Category: Mangani, S]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Beta-lactamase fold]]		[[Category: Beta-lactamase fold]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 09:53, 19 March 2014

2014-03-19T09:53:35Z

←Older revision		Revision as of 09:53, 19 March 2014
Line 4:		Line 4:

	==About this Structure==		==About this Structure==
-	[[4gzb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZB OCA].	+	[[4gzb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZB OCA].
		+
		+	==See Also==
		+	*[[Beta-lactamase\|Beta-lactamase]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023439634</ref><references group="xtra"/><references/>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
-	[[Category: ~~Pseudomonas aeruginosa pao1~~]]	+	[[Category: Pseae]]
	[[Category: Benvenuti, M.]]		[[Category: Benvenuti, M.]]
	[[Category: Docquier, J D.]]		[[Category: Docquier, J D.]]

OCA at 22:44, 10 April 2013

2013-04-10T22:44:34Z

←Older revision		Revision as of 22:44, 10 April 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4gzb\| PDB=4gzb \| SCENE= }}
		+	===Crystal structure of native AmpC beta-lactamase from Pseudomonas aeruginosa PAO1===
		+	{{ABSTRACT_PUBMED_23439634}}

-	~~The entry~~ 4gzb is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4gzb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZB OCA].
-	~~Authors~~: Benvenuti, M.~~, De Luca, F.,~~ Docquier, J.D., Mangani, S.	+	[[Category: Beta-lactamase]]
-		+	[[Category: Pseudomonas aeruginosa pao1]]
-	~~Description~~: ~~Crystal structure of native AmpC beta~~-lactamase ~~from Pseudomonas aeruginosa PAO1~~	+	[[Category: Benvenuti, M.]]
		+	[[Category: Docquier, J D.]]
		+	[[Category: Luca, F De.]]
		+	[[Category: Mangani, S.]]
		+	[[Category: Antibiotic resistance]]
		+	[[Category: Beta-lactamase fold]]
		+	[[Category: Hydrolase]]

OCA at 08:36, 31 October 2012

2012-10-31T08:36:17Z

←Older revision		Revision as of 08:36, 31 October 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4gzb is ON HOLD	+	The entry 4gzb is ON HOLD until Paper Publication

	Authors: Benvenuti, M., De Luca, F., Docquier, J.D., Mangani, S.		Authors: Benvenuti, M., De Luca, F., Docquier, J.D., Mangani, S.

	Description: Crystal structure of native AmpC beta-lactamase from Pseudomonas aeruginosa PAO1		Description: Crystal structure of native AmpC beta-lactamase from Pseudomonas aeruginosa PAO1

OCA: Protected "4gzb" [edit=sysop:move=sysop]

2012-09-12T04:57:29Z

Protected "4gzb" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:57, 12 September 2012

OCA: New page: '''Unreleased structure''' The entry 4gzb is ON HOLD Authors: Benvenuti, M., De Luca, F., Docquier, J.D., Mangani, S. Description: Crystal structure of native AmpC beta-lactamase from ...

2012-09-12T04:57:28Z

New page: '''Unreleased structure''' The entry 4gzb is ON HOLD Authors: Benvenuti, M., De Luca, F., Docquier, J.D., Mangani, S. Description: Crystal structure of native AmpC beta-lactamase from ...

New page

'''Unreleased structure'''

The entry 4gzb is ON HOLD

Authors: Benvenuti, M., De Luca, F., Docquier, J.D., Mangani, S.

Description: Crystal structure of native AmpC beta-lactamase from Pseudomonas aeruginosa PAO1