4gzz - Revision history

OCA at 16:01, 14 March 2024

2024-03-14T16:01:01Z

←Older revision		Revision as of 16:01, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gzz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GZZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gzz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GZZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.2927Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzz OCA], [https://pdbe.org/4gzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gzz RCSB], [https://www.ebi.ac.uk/pdbsum/4gzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzz OCA], [https://pdbe.org/4gzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gzz RCSB], [https://www.ebi.ac.uk/pdbsum/4gzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/RPOA_THET8 RPOA_THET8] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.		[https://www.uniprot.org/uniprot/RPOA_THET8 RPOA_THET8] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Transcriptional pausing by multisubunit RNA polymerases (RNAPs) is a key mechanism for regulating gene expression in both prokaryotes and eukaryotes and is a prerequisite for transcription termination. Pausing and termination states are thought to arise through a common, elemental pause state that is inhibitory for nucleotide addition. We report three crystal structures of Thermus RNAP elemental paused elongation complexes (ePECs). The structures reveal the same relaxed, open-clamp RNAP conformation in the ePEC that may arise by failure to re-establish DNA contacts during translocation. A kinked bridge-helix sterically blocks the RNAP active site, explaining how this conformation inhibits RNAP catalytic activity. Our results provide a framework for understanding how RNA hairpin formation stabilizes the paused state and how the ePEC intermediate facilitates termination.
-
-	Structural basis of transcriptional pausing in bacteria.,Weixlbaumer A, Leon K, Landick R, Darst SA Cell. 2013 Jan 31;152(3):431-41. doi: 10.1016/j.cell.2012.12.020. PMID:23374340<ref>PMID:23374340</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4gzz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]		*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:50, 3 November 2022

2022-11-03T07:50:29Z

←Older revision		Revision as of 07:50, 3 November 2022
Line 3:		Line 3:
	<StructureSection load='4gzz' size='340' side='right'caption='[[4gzz]], [[Resolution\|resolution]] 4.29Å' scene=''>		<StructureSection load='4gzz' size='340' side='right'caption='[[4gzz]], [[Resolution\|resolution]] 4.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gzz]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thet8 Thet8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GZZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gzz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GZZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rpoA, TTHA1664 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8]), rpoB, TTHA1813 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8]), rpoC, TTHA1812 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8]), rpoZ, TTHA1561 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzz OCA], [https://pdbe.org/4gzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gzz RCSB], [https://www.ebi.ac.uk/pdbsum/4gzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzz ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzz OCA], [~~http~~://pdbe.org/4gzz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gzz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gzz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzz ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/RPOZ_THET8 RPOZ_THET8]] Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits (By similarity). [[http://www.uniprot.org/uniprot/RPOA_THET8 RPOA_THET8]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/RPOC_THET8 RPOC_THET8]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/RPOB_THET8 RPOB_THET8]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.	+	[https://www.uniprot.org/uniprot/RPOA_THET8 RPOA_THET8] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[RNA polymerase\|RNA polymerase]]	+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: DNA-directed RNA polymerase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thet8~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Darst~~, S A~~]]	+	[[Category: Thermus thermophilus HB8]]
-	[[Category: Landick, R]]	+	[[Category: Darst SA]]
-	[[Category: Leon, K]]	+	[[Category: Landick R]]
-	[[Category: Weixlbaumer, A]]	+	[[Category: Leon K]]
-	~~[[Category: Dna directed rna transcriptionss']]~~	+	[[Category: Weixlbaumer A]]
-	~~[[Category: Paused transcription elongation complex]]~~	+
-	~~[[Category: Rna polymerase]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription-dna-rna hybrid complex]]~~	+
-	~~[[Category: Transcriptional pausing~~]]	+

OCA at 11:56, 17 July 2019

2019-07-17T11:56:29Z

←Older revision		Revision as of 11:56, 17 July 2019
Line 1:		Line 1:

	==Crystal structures of bacterial RNA Polymerase paused elongation complexes==		==Crystal structures of bacterial RNA Polymerase paused elongation complexes==
-	<StructureSection load='4gzz' size='340' side='right' caption='[[4gzz]], [[Resolution\|resolution]] 4.29Å' scene=''>	+	<StructureSection load='4gzz' size='340' side='right'caption='[[4gzz]], [[Resolution\|resolution]] 4.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gzz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GZZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gzz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GZZ FirstGlance]. <br>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4gzz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gzz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA polymerase\|RNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: DNA-directed RNA polymerase]]		[[Category: DNA-directed RNA polymerase]]
		+	[[Category: Large Structures]]
	[[Category: Thet8]]		[[Category: Thet8]]
	[[Category: Darst, S A]]		[[Category: Darst, S A]]
Line 30:		Line 34:
	[[Category: Leon, K]]		[[Category: Leon, K]]
	[[Category: Weixlbaumer, A]]		[[Category: Weixlbaumer, A]]
-	[[Category: Dna directed rna ~~transcriptions~~']]	+	[[Category: Dna directed rna transcriptionss']]
	[[Category: Paused transcription elongation complex]]		[[Category: Paused transcription elongation complex]]
	[[Category: Rna polymerase]]		[[Category: Rna polymerase]]

OCA at 08:34, 15 November 2017

2017-11-15T08:34:36Z

←Older revision		Revision as of 08:34, 15 November 2017
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4gzz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gzz" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[RNA polymerase\|RNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:36, 5 August 2016

2016-08-05T20:36:55Z

←Older revision		Revision as of 20:36, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structures of bacterial RNA Polymerase paused elongation complexes==		==Crystal structures of bacterial RNA Polymerase paused elongation complexes==
	<StructureSection load='4gzz' size='340' side='right' caption='[[4gzz]], [[Resolution\|resolution]] 4.29Å' scene=''>		<StructureSection load='4gzz' size='340' side='right' caption='[[4gzz]], [[Resolution\|resolution]] 4.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gzz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus_hb8 Thermus thermophilus hb8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GZZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gzz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GZZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rpoA, TTHA1664 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 ~~Thermus thermophilus HB8~~]), rpoB, TTHA1813 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 ~~Thermus thermophilus HB8~~]), rpoC, TTHA1812 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 ~~Thermus thermophilus HB8~~]), rpoZ, TTHA1561 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 ~~Thermus thermophilus HB8~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rpoA, TTHA1664 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8]), rpoB, TTHA1813 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8]), rpoC, TTHA1812 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8]), rpoZ, TTHA1561 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gzz RCSB], [http://www.ebi.ac.uk/pdbsum/4gzz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzz OCA], [http://pdbe.org/4gzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gzz RCSB], [http://www.ebi.ac.uk/pdbsum/4gzz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4gzz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: DNA-directed RNA polymerase]]		[[Category: DNA-directed RNA polymerase]]
-	[[Category: ~~Thermus thermophilus hb8~~]]	+	[[Category: Thet8]]
	[[Category: Darst, S A]]		[[Category: Darst, S A]]
	[[Category: Landick, R]]		[[Category: Landick, R]]

OCA at 21:42, 25 December 2014

2014-12-25T21:42:46Z

←Older revision		Revision as of 21:42, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gzz RCSB], [http://www.ebi.ac.uk/pdbsum/4gzz PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gzz RCSB], [http://www.ebi.ac.uk/pdbsum/4gzz PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RPOZ_THET8 RPOZ_THET8]] Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits (By similarity). [[http://www.uniprot.org/uniprot/RPOA_THET8 RPOA_THET8]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/RPOC_THET8 RPOC_THET8]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/RPOB_THET8 RPOB_THET8]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:28, 10 December 2014

2014-12-10T09:28:34Z

←Older revision		Revision as of 09:28, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4gzz.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Crystal structures of bacterial RNA Polymerase paused elongation complexes==
		+	<StructureSection load='4gzz' size='340' side='right' caption='[[4gzz]], [[Resolution\|resolution]] 4.29Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4gzz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GZZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rpoA, TTHA1664 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8]), rpoB, TTHA1813 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8]), rpoC, TTHA1812 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8]), rpoZ, TTHA1561 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gzz RCSB], [http://www.ebi.ac.uk/pdbsum/4gzz PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transcriptional pausing by multisubunit RNA polymerases (RNAPs) is a key mechanism for regulating gene expression in both prokaryotes and eukaryotes and is a prerequisite for transcription termination. Pausing and termination states are thought to arise through a common, elemental pause state that is inhibitory for nucleotide addition. We report three crystal structures of Thermus RNAP elemental paused elongation complexes (ePECs). The structures reveal the same relaxed, open-clamp RNAP conformation in the ePEC that may arise by failure to re-establish DNA contacts during translocation. A kinked bridge-helix sterically blocks the RNAP active site, explaining how this conformation inhibits RNAP catalytic activity. Our results provide a framework for understanding how RNA hairpin formation stabilizes the paused state and how the ePEC intermediate facilitates termination.

-	~~{{STRUCTURE_4gzz\| PDB=4gzz \| SCENE= }}~~	+	Structural basis of transcriptional pausing in bacteria.,Weixlbaumer A, Leon K, Landick R, Darst SA Cell. 2013 Jan 31;152(3):431-41. doi: 10.1016/j.cell.2012.12.020. PMID:23374340<ref>PMID:23374340</ref>

-	~~===Crystal structures~~ of ~~bacterial RNA Polymerase paused elongation complexes===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_23374340}}~~	+	==See Also==
-		+	*[[RNA polymerase\|RNA polymerase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4gzz~~]] ~~is a 8 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA].~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: DNA-directed RNA polymerase]]		[[Category: DNA-directed RNA polymerase]]
	[[Category: Thermus thermophilus hb8]]		[[Category: Thermus thermophilus hb8]]
-	[[Category: Darst, S A.]]	+	[[Category: Darst, S A]]
-	[[Category: Landick, R.]]	+	[[Category: Landick, R]]
-	[[Category: Leon, K.]]	+	[[Category: Leon, K]]
-	[[Category: Weixlbaumer, A.]]	+	[[Category: Weixlbaumer, A]]
	[[Category: Dna directed rna transcriptions']]		[[Category: Dna directed rna transcriptions']]
	[[Category: Paused transcription elongation complex]]		[[Category: Paused transcription elongation complex]]

OCA at 10:34, 13 February 2013

2013-02-13T10:34:14Z

←Older revision		Revision as of 10:34, 13 February 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4gzz.jpg\|left\|200px]]

-	~~The entry~~ 4gzz ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4gzz\| PDB=4gzz \| SCENE= }}

-	~~Authors: Weixlbaumer, A., Leon, K., Landick, R., Darst, S.A.~~	+	===Crystal structures of bacterial RNA Polymerase paused elongation complexes===

-	~~Description~~: ~~Crystal structures of bacterial~~ RNA ~~Polymerase paused~~ elongation ~~complexes~~	+	{{ABSTRACT_PUBMED_23374340}}
		+
		+	==About this Structure==
		+	[[4gzz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZZ OCA].
		+	[[Category: DNA-directed RNA polymerase]]
		+	[[Category: Thermus thermophilus hb8]]
		+	[[Category: Darst, S A.]]
		+	[[Category: Landick, R.]]
		+	[[Category: Leon, K.]]
		+	[[Category: Weixlbaumer, A.]]
		+	[[Category: Dna directed rna transcriptions']]
		+	[[Category: Paused transcription elongation complex]]
		+	[[Category: Rna polymerase]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription-dna-rna hybrid complex]]
		+	[[Category: Transcriptional pausing]]

OCA at 08:36, 31 October 2012

2012-10-31T08:36:23Z

←Older revision		Revision as of 08:36, 31 October 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4gzz is ON HOLD	+	The entry 4gzz is ON HOLD until Paper Publication

	Authors: Weixlbaumer, A., Leon, K., Landick, R., Darst, S.A.		Authors: Weixlbaumer, A., Leon, K., Landick, R., Darst, S.A.

	Description: Crystal structures of bacterial RNA Polymerase paused elongation complexes		Description: Crystal structures of bacterial RNA Polymerase paused elongation complexes

OCA: Protected "4gzz" [edit=sysop:move=sysop]

2012-09-12T04:58:10Z

Protected "4gzz" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:58, 12 September 2012