4h1y - Revision history

OCA at 08:35, 5 August 2016

2016-08-05T08:35:58Z

←Older revision		Revision as of 08:35, 5 August 2016
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		+
	==Human ecto-5'-nucleotidase (CD73): crystal form II (open) in complex with PSB11552==		==Human ecto-5'-nucleotidase (CD73): crystal form II (open) in complex with PSB11552==
	<StructureSection load='4h1y' size='340' side='right' caption='[[4h1y]], [[Resolution\|resolution]] 1.58Å' scene=''>		<StructureSection load='4h1y' size='340' side='right' caption='[[4h1y]], [[Resolution\|resolution]] 1.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h1y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h1y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1Y FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0YQ:2,2-[(2-{[2-({[(2S,3S,4R,5R)-5-(2,4-DIOXO-3,4-DIHYDROPYRIMIDIN-1(2H)-YL)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]CARBONYL}AMINO)ETHYL]AMINO}-2-OXOETHYL)IMINO]DIACETIC+ACID+(NON-PREFERRED+NAME)'>0YQ</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0YQ:2,2-[(2-{[2-({[(2S,3S,4R,5R)-5-(2,4-DIOXO-3,4-DIHYDROPYRIMIDIN-1(2H)-YL)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]CARBONYL}AMINO)ETHYL]AMINO}-2-OXOETHYL)IMINO]DIACETIC+ACID+(NON-PREFERRED+NAME)'>0YQ</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4h2b\|4h2b]], [[4h2f\|4h2f]], [[4h2i\|4h2i]], [[4h2g\|4h2g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4h2b\|4h2b]], [[4h2f\|4h2f]], [[4h2i\|4h2i]], [[4h2g\|4h2g]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NT5E, NT5, NTE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NT5E, NT5, NTE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h1y RCSB], [http://www.ebi.ac.uk/pdbsum/4h1y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1y OCA], [http://pdbe.org/4h1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h1y RCSB], [http://www.ebi.ac.uk/pdbsum/4h1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h1y ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4h1y" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Knapp, K]]		[[Category: Knapp, K]]
	[[Category: Pippel, J]]		[[Category: Pippel, J]]

OCA at 17:20, 25 December 2014

2014-12-25T17:20:01Z

←Older revision		Revision as of 17:20, 25 December 2014
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-	[[~~Image:~~4h1y.~~png~~\|~~left~~\|~~200px~~]]	+	==Human ecto-5'-nucleotidase (CD73): crystal form II (open) in complex with PSB11552==
		+	<StructureSection load='4h1y' size='340' side='right' caption='[[4h1y]], [[Resolution\|resolution]] 1.58Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4h1y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1Y FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0YQ:2,2-[(2-{[2-({[(2S,3S,4R,5R)-5-(2,4-DIOXO-3,4-DIHYDROPYRIMIDIN-1(2H)-YL)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]CARBONYL}AMINO)ETHYL]AMINO}-2-OXOETHYL)IMINO]DIACETIC+ACID+(NON-PREFERRED+NAME)'>0YQ</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4h2b\|4h2b]], [[4h2f\|4h2f]], [[4h2i\|4h2i]], [[4h2g\|4h2g]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NT5E, NT5, NTE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h1y RCSB], [http://www.ebi.ac.uk/pdbsum/4h1y PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/5NTD_HUMAN 5NTD_HUMAN]] Hereditary arterial and articular multiple calcification syndrome. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/5NTD_HUMAN 5NTD_HUMAN]] Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.<ref>PMID:21933152</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In vertebrates ecto-5'-nucleotidase (e5NT) catalyzes the hydrolysis of extracellular AMP to adenosine and represents the major control point for extracellular adenosine levels. Due to its pivotal role for activation of P1 adenosine receptors, e5NT has emerged as an appealing drug target for treatment of inflammation, chronic pain, hypoxia, and cancer. Crystal structures of the dimeric human e5NT reveal an extensive 114 degrees conformational switch between the open and closed forms of the enzyme. The dimerization interface is formed by the C-terminal domains and exhibits interchain motions of up to 13 degrees . Complex structures with adenosine and AMPCP indicate that structural control of the domain movement determines the selectivity for monophosphate nucleotides. Binding modes of nucleotide-derived and flavonoid-based compounds complexed to the C-terminal domain in the open form reveal an additional binding pocket of approximately 210 A(3) that might be explored to design more potent inhibitors.

-	~~{{STRUCTURE_4h1y\| PDB=4h1y \| SCENE= }}~~	+	Crystal Structure of the Human Ecto-5'-Nucleotidase (CD73): Insights into the Regulation of Purinergic Signaling.,Knapp K, Zebisch M, Pippel J, El-Tayeb A, Muller CE, Strater N Structure. 2012 Nov 6. pii: S0969-2126(12)00375-9. doi:, 10.1016/j.str.2012.10.001. PMID:23142347<ref>PMID:23142347</ref>

-	~~===Human ecto-5'-nucleotidase (CD73): crystal form II (open) in complex with PSB11552===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23142347}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4h1y]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1Y OCA].~~	+	</StructureSection>
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Knapp, K.]]	+	[[Category: Knapp, K]]
-	[[Category: Pippel, J.]]	+	[[Category: Pippel, J]]
-	[[Category: Straeter, N.]]	+	[[Category: Straeter, N]]
-	[[Category: Zebisch, M.]]	+	[[Category: Zebisch, M]]
	[[Category: Amp binding]]		[[Category: Amp binding]]
	[[Category: Dimeric]]		[[Category: Dimeric]]

OCA at 07:03, 2 January 2013

2013-01-02T07:03:12Z

←Older revision		Revision as of 07:03, 2 January 2013
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-	[[Image:4h1y.~~jpg~~\|left\|200px]]	+	[[Image:4h1y.png\|left\|200px]]

	{{STRUCTURE_4h1y\| PDB=4h1y \| SCENE= }}		{{STRUCTURE_4h1y\| PDB=4h1y \| SCENE= }}

OCA at 14:24, 28 November 2012

2012-11-28T14:24:09Z

←Older revision		Revision as of 14:24, 28 November 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:4h1y.jpg\|left\|200px]]

-	~~The entry~~ 4h1y ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4h1y\| PDB=4h1y \| SCENE= }}

-	~~Authors~~: ~~Pippel, J., Zebisch, M., Knapp, K., Straeter, N.~~	+	===Human ecto-5'-nucleotidase (CD73): crystal form II (open) in complex with PSB11552===

-	~~Description~~: ~~Phosphatase~~, ~~crystal form II+PSB~~	+	{{ABSTRACT_PUBMED_23142347}}
		+
		+	==About this Structure==
		+	[[4h1y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1Y OCA].
		+	[[Category: 5'-nucleotidase]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Knapp, K.]]
		+	[[Category: Pippel, J.]]
		+	[[Category: Straeter, N.]]
		+	[[Category: Zebisch, M.]]
		+	[[Category: Amp binding]]
		+	[[Category: Dimeric]]
		+	[[Category: Extracellular]]
		+	[[Category: Hydrolase]]
		+	[[Category: Nucleotidase]]
		+	[[Category: Zinc binding]]

OCA at 20:10, 26 September 2012

2012-09-26T20:10:50Z

←Older revision		Revision as of 20:10, 26 September 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4h1y is ON HOLD	+	The entry 4h1y is ON HOLD until Paper Publication

	Authors: Pippel, J., Zebisch, M., Knapp, K., Straeter, N.		Authors: Pippel, J., Zebisch, M., Knapp, K., Straeter, N.

	Description: Phosphatase, crystal form II+PSB		Description: Phosphatase, crystal form II+PSB

OCA: Protected "4h1y" [edit=sysop:move=sysop]

2012-09-19T06:57:58Z

Protected "4h1y" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:57, 19 September 2012

OCA: New page: '''Unreleased structure''' The entry 4h1y is ON HOLD Authors: Pippel, J., Zebisch, M., Knapp, K., Straeter, N. Description: Phosphatase, crystal form II+PSB

2012-09-19T06:57:57Z

New page: '''Unreleased structure''' The entry 4h1y is ON HOLD Authors: Pippel, J., Zebisch, M., Knapp, K., Straeter, N. Description: Phosphatase, crystal form II+PSB

New page

'''Unreleased structure'''

The entry 4h1y is ON HOLD

Authors: Pippel, J., Zebisch, M., Knapp, K., Straeter, N.

Description: Phosphatase, crystal form II+PSB