4h6i - Revision history

OCA at 15:00, 20 September 2023

2023-09-20T15:00:58Z

←Older revision		Revision as of 15:00, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4h6i]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H6I FirstGlance]. <br>		<table><tr><td colspan='2'>[[4h6i]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H6I FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h6i OCA], [https://pdbe.org/4h6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h6i RCSB], [https://www.ebi.ac.uk/pdbsum/4h6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h6i ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.091Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h6i OCA], [https://pdbe.org/4h6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h6i RCSB], [https://www.ebi.ac.uk/pdbsum/4h6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h6i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:02, 3 November 2022

2022-11-03T08:02:16Z

←Older revision		Revision as of 08:02, 3 November 2022
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	==Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B==		==Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B==
-	<StructureSection load='4h6i' size='340' side='right' caption='[[4h6i]], [[Resolution\|resolution]] 3.09Å' scene=''>	+	<StructureSection load='4h6i' size='340' side='right'caption='[[4h6i]], [[Resolution\|resolution]] 3.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h6i]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Staam Staam~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4H6I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h6i]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H6I FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t49\|3t49]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h6i OCA], [https://pdbe.org/4h6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h6i RCSB], [https://www.ebi.ac.uk/pdbsum/4h6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h6i ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV1159, SAV_1159 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4h6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h6i OCA], [~~http~~://pdbe.org/4h6i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4h6i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4h6i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4h6i ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0H3JZ23_STAAM A0A0H3JZ23_STAAM] Involved in countering the first line of host defense mechanisms. Efficiently inhibits opsonization, phagocytosis and killing of S.aureus by human neutrophils. Acts by binding and stabilizing human C3 convertases (C4b2a and C3bBb), leading to their inactivation. The convertases are no longer able to cleave complement C3, therefore preventing further C3b deposition on the bacterial surface and phagocytosis of the bacterium. Also prevents C5a-induced neutrophil responses.[ARBA:ARBA00025334]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4h6i" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4h6i" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Fibrinogen binding protein\|Fibrinogen binding protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Staam~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Garcia, B L~~]]	+	[[Category: Staphylococcus aureus subsp. aureus Mu50]]
-	[[Category: ~~Geisbrecht, B V~~]]	+	[[Category: Garcia BL]]
-	[[Category: ~~Complement system]]~~	+	[[Category: Geisbrecht BV]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Innate immunity]]~~	+
-	~~[[Category: Staphylococcus aureus structural biology~~]]	+

OCA at 08:36, 15 November 2017

2017-11-15T08:36:34Z

←Older revision		Revision as of 08:36, 15 November 2017
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	</div>		</div>
	<div class="pdbe-citations 4h6i" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4h6i" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Fibrinogen binding protein\|Fibrinogen binding protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:56, 15 December 2016

2016-12-15T19:56:42Z

←Older revision		Revision as of 19:56, 15 December 2016
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		+
	==Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B==		==Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B==
	<StructureSection load='4h6i' size='340' side='right' caption='[[4h6i]], [[Resolution\|resolution]] 3.09Å' scene=''>		<StructureSection load='4h6i' size='340' side='right' caption='[[4h6i]], [[Resolution\|resolution]] 3.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h6i]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H6I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h6i]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H6I FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t49\|3t49]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t49\|3t49]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV1159, SAV_1159 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 ~~Staphylococcus aureus subsp. aureus Mu50~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV1159, SAV_1159 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h6i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h6i RCSB], [http://www.ebi.ac.uk/pdbsum/4h6i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h6i OCA], [http://pdbe.org/4h6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h6i RCSB], [http://www.ebi.ac.uk/pdbsum/4h6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h6i ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4h6i" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 22:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Staphylococcus aureus subsp. aureus mu50~~]]	+	[[Category: Staam]]
	[[Category: Garcia, B L]]		[[Category: Garcia, B L]]
	[[Category: Geisbrecht, B V]]		[[Category: Geisbrecht, B V]]

OCA at 09:25, 10 December 2014

2014-12-10T09:25:19Z

←Older revision		Revision as of 09:25, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4h6i.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B==
		+	<StructureSection load='4h6i' size='340' side='right' caption='[[4h6i]], [[Resolution\|resolution]] 3.09Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4h6i]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H6I FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t49\|3t49]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV1159, SAV_1159 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 Staphylococcus aureus subsp. aureus Mu50])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h6i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h6i RCSB], [http://www.ebi.ac.uk/pdbsum/4h6i PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Complement is a network of interacting circulatory and cell-surface proteins that recognizes, marks, and facilitates clearance of microbial invaders. To evade complement attack, the pathogenic organism Staphylococcus aureus expresses a number of secreted proteins that interfere with activation and regulation of the complement cascade. Staphylococcal Complement Inhibitors (SCINs) are one important class of these immunomodulators and consist of three active members (SCIN-A/-B/-C). SCINs inhibit a critical enzymatic complex, the alternative pathway C3 convertase, by targeting a functional hotspot on the central opsonin of complement, C3b. Although N-terminal truncation mutants of SCINs retain complement inhibitory properties they are significantly weaker binders of C3b. To provide a structural basis for this observation, we undertook a series of crystallographic and NMR dynamics studies on full-length SCINs. This work reveals that N-terminal SCIN domains are characterized by a conformationally dynamic helical motif. C3b-binding and functional experiments further demonstrate that this sequence divergent N-terminal region of SCINs is both functionally important and context-dependent. Finally, surface plasmon resonance data provide evidence for the formation of inhibitor/enzyme/substrate complexes ((SCIN/C3bBb)/C3). Similar to the (SCIN/C3bBb)2 psuedo-dimeric complexes, ((SCIN/C3bBb)/C3) interferes with the interaction of complement receptors and C3b. This activity provides an additional mechanism by which SCIN couples convertase inhibition to direct blocking of phagocytosis. Together these data suggest that tethering multi-host-protein complexes by small modular bacterial inhibitors may be a global strategy of immune evasion employed by S. aureus. The work presented here provides detailed structure-activity relationships and improves our understanding of how S. aureus circumvents human innate immunity.

-	~~{{STRUCTURE_4h6i\| PDB=4h6i \| SCENE= }}~~	+	A structurally dynamic N-terminal helix is a key functional determinant in Staphylococcal Complement Inhibitor (SCIN) proteins.,Garcia BL, Summers BJ, Ramyar KX, Tzekou A, Lin Z, Ricklin D, Lambris JD, Laity JH, Geisbrecht BV J Biol Chem. 2012 Dec 11. PMID:23233676<ref>PMID:23233676</ref>

-	~~===Crystal Structure~~ of ~~Staphylococcal Complement Inhibitor SCIN-B===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_23233676}}~~	+	==See Also==
-		+	*[[Fibrinogen binding protein\|Fibrinogen binding protein]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4h6i~~]] ~~is a 3 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6I OCA].	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Staphylococcus aureus subsp. aureus mu50]]		[[Category: Staphylococcus aureus subsp. aureus mu50]]
-	[[Category: Garcia, B L.]]	+	[[Category: Garcia, B L]]
-	[[Category: Geisbrecht, B V.]]	+	[[Category: Geisbrecht, B V]]
	[[Category: Complement system]]		[[Category: Complement system]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Innate immunity]]		[[Category: Innate immunity]]
	[[Category: Staphylococcus aureus structural biology]]		[[Category: Staphylococcus aureus structural biology]]

OCA at 07:35, 2 January 2013

2013-01-02T07:35:52Z

←Older revision		Revision as of 07:35, 2 January 2013
Line 1:		Line 1:
-	[[Image:4h6i.~~jpg~~\|left\|200px]]	+	[[Image:4h6i.png\|left\|200px]]

	{{STRUCTURE_4h6i\| PDB=4h6i \| SCENE= }}		{{STRUCTURE_4h6i\| PDB=4h6i \| SCENE= }}
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	===Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B===		===Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B===

		+	{{ABSTRACT_PUBMED_23233676}}

	==About this Structure==		==About this Structure==

OCA at 11:38, 19 December 2012

2012-12-19T11:38:03Z

←Older revision		Revision as of 11:38, 19 December 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4h6i.jpg\|left\|200px]]

-	~~The entry~~ 4h6i ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4h6i\| PDB=4h6i \| SCENE= }}

-	~~Authors: Garcia,~~ B~~.L., Geisbrecht, B.V.~~	+	===Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B===

-	~~Description: Crystal~~ Structure ~~of Staphylococcal Complement Inhibitor SCIN~~-B	+
		+	==About this Structure==
		+	[[4h6i]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6I OCA].
		+	[[Category: Staphylococcus aureus subsp. aureus mu50]]
		+	[[Category: Garcia, B L.]]
		+	[[Category: Geisbrecht, B V.]]
		+	[[Category: Complement system]]
		+	[[Category: Immune system]]
		+	[[Category: Innate immunity]]
		+	[[Category: Staphylococcus aureus structural biology]]

OCA at 05:48, 3 October 2012

2012-10-03T05:48:20Z

←Older revision		Revision as of 05:48, 3 October 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4h6i is ON HOLD	+	The entry 4h6i is ON HOLD until Paper Publication

	Authors: Garcia, B.L., Geisbrecht, B.V.		Authors: Garcia, B.L., Geisbrecht, B.V.

	Description: Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B		Description: Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B

OCA: Protected "4h6i" [edit=sysop:move=sysop]

2012-09-26T20:14:13Z

Protected "4h6i" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:14, 26 September 2012

OCA: New page: '''Unreleased structure''' The entry 4h6i is ON HOLD Authors: Garcia, B.L., Geisbrecht, B.V. Description: Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B

2012-09-26T20:14:12Z

New page: '''Unreleased structure''' The entry 4h6i is ON HOLD Authors: Garcia, B.L., Geisbrecht, B.V. Description: Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B

New page

'''Unreleased structure'''

The entry 4h6i is ON HOLD

Authors: Garcia, B.L., Geisbrecht, B.V.

Description: Crystal Structure of Staphylococcal Complement Inhibitor SCIN-B