4h8n - Revision history

OCA at 14:06, 8 November 2023

2023-11-08T14:06:33Z

←Older revision		Revision as of 14:06, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4h8n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_parapsilosis Candida parapsilosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H8N FirstGlance]. <br>		<table><tr><td colspan='2'>[[4h8n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_parapsilosis Candida parapsilosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H8N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h8n OCA], [https://pdbe.org/4h8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h8n RCSB], [https://www.ebi.ac.uk/pdbsum/4h8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h8n ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h8n OCA], [https://pdbe.org/4h8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h8n RCSB], [https://www.ebi.ac.uk/pdbsum/4h8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h8n ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:05, 3 November 2022

2022-11-03T08:05:07Z

←Older revision		Revision as of 08:05, 3 November 2022
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	==Crystal structure of conjugated polyketone reductase C2 from candida parapsilosis complexed with NADPH==		==Crystal structure of conjugated polyketone reductase C2 from candida parapsilosis complexed with NADPH==
-	<StructureSection load='4h8n' size='340' side='right' caption='[[4h8n]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='4h8n' size='340' side='right'caption='[[4h8n]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h8n]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_22019 Atcc 22019~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4H8N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h8n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_parapsilosis Candida parapsilosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H8N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vxg\|3vxg]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h8n OCA], [https://pdbe.org/4h8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h8n RCSB], [https://www.ebi.ac.uk/pdbsum/4h8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h8n ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cpr-c2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5480 ATCC 22019])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-dehydropantolactone_reductase_((Si)-specific) 2-dehydropantolactone reductase ((Si)-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.214 1.1.1.214] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4h8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h8n OCA], [~~http~~://pdbe.org/4h8n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4h8n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4h8n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4h8n ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CPRC2_CANPA CPRC2_CANPA] NADPH-dependent conjugated polyketone reductase with broad substrate specificity and strict stereospecificity. Reduces ketopantoyl lactone and isatin. Does not act on menadione, p-nitrobenzaldehyde and pyridine-3-aldehyde.<ref>PMID:11826979</ref> <ref>PMID:14593510</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 22019~~]]	+	[[Category: Candida parapsilosis]]
-	[[Category: Kataoka, M]]	+	[[Category: Large Structures]]
-	[[Category: Maruoka, S]]	+	[[Category: Kataoka M]]
-	[[Category: Miyakawa, T]]	+	[[Category: Maruoka S]]
-	[[Category: Nagata, K]]	+	[[Category: Miyakawa T]]
-	[[Category: Ohtsuka, J]]	+	[[Category: Nagata K]]
-	[[Category: Qin, H M]]	+	[[Category: Ohtsuka J]]
-	[[Category: Shimizu, S]]	+	[[Category: Qin H-M]]
-	[[Category: Tanokura, M]]	+	[[Category: Shimizu S]]
-	[[Category: Yamamura, A]]	+	[[Category: Tanokura M]]
-	~~[[Category: D-pantoyl lactone]]~~	+	[[Category: Yamamura A]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 15:30, 11 August 2016

2016-08-11T15:30:31Z

←Older revision		Revision as of 15:30, 11 August 2016
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-	{{STRUCTURE_4h8n\| PDB=4h8n \| SCENE= }}
-	===Crystal structure of conjugated polyketone reductase C2 from candida parapsilosis complexed with NADPH===
-	{{ABSTRACT_PUBMED_23828603}}

-	==~~About this Structure~~==	+	==Crystal structure of conjugated polyketone reductase C2 from candida parapsilosis complexed with NADPH==
-	[[4h8n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_22019 Atcc 22019]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8N OCA].	+	<StructureSection load='4h8n' size='340' side='right' caption='[[4h8n]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4h8n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_22019 Atcc 22019]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H8N FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vxg\|3vxg]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cpr-c2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5480 ATCC 22019])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-dehydropantolactone_reductase_((Si)-specific) 2-dehydropantolactone reductase ((Si)-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.214 1.1.1.214] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h8n OCA], [http://pdbe.org/4h8n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h8n RCSB], [http://www.ebi.ac.uk/pdbsum/4h8n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h8n ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Conjugated polyketone reductase C2 (CPR-C2) from Candida parapsilosis IFO 0708, identified as a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent ketopantoyl lactone reductase, belongs to the aldo-keto reductase superfamily. This enzyme reduces ketopantoyl lactone to D-pantoyl lactone in a strictly stereospecific manner. To elucidate the structural basis of the substrate specificity, we determined the crystal structures of the apo CPR-C2 and CPR-C2/NADPH complex at 1.70 and 1.80 A resolutions, respectively. CPR-C2 adopted a triose-phosphate isomerase barrel fold at the core of the structure. Binding with the cofactor NADPH induced conformational changes in which Thr27 and Lys28 moved 15 and 5.0 A, respectively, in the close vicinity of the adenosine 2'-phosphate group of NADPH to form hydrogen bonds. Based on the comparison of the CPR-C2/NADPH structure with 3-alpha-hydroxysteroid dehydrogenase and mutation analyses, we constructed substrate binding models with ketopantoyl lactone, which provided insight into the substrate specificity by the cofactor-induced structure. The results will be useful for the rational design of CPR-C2 mutants targeted for use in the industrial manufacture of ketopantoyl lactone.

-	~~==Reference==~~	+	Structure of conjugated polyketone reductase from Candida parapsilosis IFO 0708 reveals conformational changes for substrate recognition upon NADPH binding.,Qin HM, Yamamura A, Miyakawa T, Kataoka M, Nagai T, Kitamura N, Urano N, Maruoka S, Ohtsuka J, Nagata K, Shimizu S, Tanokura M Appl Microbiol Biotechnol. 2013 Jul 5. PMID:23828603<ref>PMID:23828603</ref>
-	<ref ~~group="xtra"~~>PMID:~~023828603~~</ref><~~references group~~="~~xtra~~"/><references/>	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4h8n" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Atcc 22019]]		[[Category: Atcc 22019]]
-	[[Category: Kataoka, M.]]	+	[[Category: Kataoka, M]]
-	[[Category: Maruoka, S.]]	+	[[Category: Maruoka, S]]
-	[[Category: Miyakawa, T.]]	+	[[Category: Miyakawa, T]]
-	[[Category: Nagata, K.]]	+	[[Category: Nagata, K]]
-	[[Category: Ohtsuka, J.]]	+	[[Category: Ohtsuka, J]]
-	[[Category: Qin, H M.]]	+	[[Category: Qin, H M]]
-	[[Category: Shimizu, S.]]	+	[[Category: Shimizu, S]]
-	[[Category: Tanokura, M.]]	+	[[Category: Tanokura, M]]
-	[[Category: Yamamura, A.]]	+	[[Category: Yamamura, A]]
	[[Category: D-pantoyl lactone]]		[[Category: D-pantoyl lactone]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

OCA at 06:45, 15 January 2014

2014-01-15T06:45:38Z

←Older revision		Revision as of 06:45, 15 January 2014
Line 4:		Line 4:

	==About this Structure==		==About this Structure==
-	[[4h8n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Candida_parapsilosis Candida parapsilosis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8N OCA].	+	[[4h8n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_22019 Atcc 22019]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8N OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:023828603</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:023828603</ref><references group="xtra"/><references/>
-	[[Category: ~~Candida parapsilosis~~]]	+	[[Category: Atcc 22019]]
	[[Category: Kataoka, M.]]		[[Category: Kataoka, M.]]
	[[Category: Maruoka, S.]]		[[Category: Maruoka, S.]]

OCA at 20:51, 7 August 2013

2013-08-07T20:51:45Z

←Older revision		Revision as of 20:51, 7 August 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4h8n\| PDB=4h8n \| SCENE= }}
		+	===Crystal structure of conjugated polyketone reductase C2 from candida parapsilosis complexed with NADPH===
		+	{{ABSTRACT_PUBMED_23828603}}

-	~~The entry~~ 4h8n is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4h8n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_parapsilosis Candida parapsilosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H8N OCA].

-	~~Authors~~: ~~Yamamura~~, A., Miyakawa, T., ~~Maruoka, S~~., Ohtsuka, J., ~~Nagata, K., Kataoka,~~ M., Shimizu, S., Tanokura, M.	+	==Reference==
-		+	<ref group="xtra">PMID:023828603</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal structure of conjugated polyketone reductase C2 from candida parapsilosis complexed with NADPH~~	+	[[Category: Candida parapsilosis]]
		+	[[Category: Kataoka, M.]]
		+	[[Category: Maruoka, S.]]
		+	[[Category: Miyakawa, T.]]
		+	[[Category: Nagata, K.]]
		+	[[Category: Ohtsuka, J.]]
		+	[[Category: Qin, H M.]]
		+	[[Category: Shimizu, S.]]
		+	[[Category: Tanokura, M.]]
		+	[[Category: Yamamura, A.]]
		+	[[Category: D-pantoyl lactone]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Tim barrel]]

OCA at 10:58, 19 December 2012

2012-12-19T10:58:17Z

←Older revision		Revision as of 10:58, 19 December 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4h8n is ON HOLD	+	The entry 4h8n is ON HOLD until Paper Publication

	Authors: Yamamura, A., Miyakawa, T., Maruoka, S., Ohtsuka, J., Nagata, K., Kataoka, M., Shimizu, S., Tanokura, M.		Authors: Yamamura, A., Miyakawa, T., Maruoka, S., Ohtsuka, J., Nagata, K., Kataoka, M., Shimizu, S., Tanokura, M.

	Description: Crystal structure of conjugated polyketone reductase C2 from candida parapsilosis complexed with NADPH		Description: Crystal structure of conjugated polyketone reductase C2 from candida parapsilosis complexed with NADPH

OCA: Protected "4h8n" [edit=sysop:move=sysop]

2012-10-03T05:49:41Z

Protected "4h8n" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:49, 3 October 2012

OCA: New page: '''Unreleased structure''' The entry 4h8n is ON HOLD Authors: Yamamura, A., Miyakawa, T., Maruoka, S., Ohtsuka, J., Nagata, K., Kataoka, M., Shimizu, S., Tanokura, M. Description: Crys...

2012-10-03T05:49:40Z

New page: '''Unreleased structure''' The entry 4h8n is ON HOLD Authors: Yamamura, A., Miyakawa, T., Maruoka, S., Ohtsuka, J., Nagata, K., Kataoka, M., Shimizu, S., Tanokura, M. Description: Crys...

New page

'''Unreleased structure'''

The entry 4h8n is ON HOLD

Authors: Yamamura, A., Miyakawa, T., Maruoka, S., Ohtsuka, J., Nagata, K., Kataoka, M., Shimizu, S., Tanokura, M.

Description: Crystal structure of conjugated polyketone reductase C2 from candida parapsilosis complexed with NADPH