4hbo - Revision history

OCA at 15:03, 20 September 2023

2023-09-20T15:03:02Z

←Older revision		Revision as of 15:03, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hbo]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Rubella_virus_strain_M33 Rubella virus strain M33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HBO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hbo]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Rubella_virus_strain_M33 Rubella virus strain M33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HBO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.241Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hbo OCA], [https://pdbe.org/4hbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hbo RCSB], [https://www.ebi.ac.uk/pdbsum/4hbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hbo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hbo OCA], [https://pdbe.org/4hbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hbo RCSB], [https://www.ebi.ac.uk/pdbsum/4hbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hbo ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:08, 3 November 2022

2022-11-03T08:08:54Z

←Older revision		Revision as of 08:08, 3 November 2022
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	==Crystal Structure of Rubella virus capsid protein (residues 127-277)==		==Crystal Structure of Rubella virus capsid protein (residues 127-277)==
-	<StructureSection load='4hbo' size='340' side='right' caption='[[4hbo]], [[Resolution\|resolution]] 3.24Å' scene=''>	+	<StructureSection load='4hbo' size='340' side='right'caption='[[4hbo]], [[Resolution\|resolution]] 3.24Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hbo]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Rubv Rubv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HBO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HBO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hbo]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Rubella_virus_strain_M33 Rubella virus strain M33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HBO FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4har\|4har]], [[4hbe\|4hbe]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hbo OCA], [https://pdbe.org/4hbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hbo RCSB], [https://www.ebi.ac.uk/pdbsum/4hbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hbo ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Capsid ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11043 RUBV])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hbo OCA], [~~http~~://pdbe.org/4hbo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hbo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hbo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hbo ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/POLS_RUBVM POLS_RUBVM] Capsid protein interacts with genomic RNA and assembles into icosaedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions. Phosphorylation negatively regulates RNA-binding activity, possibly delaying virion assembly during the viral replication phase. Capsid protein dimerizes and becomes disulfide-linked in the virion, but this interaction seems not to be important for its biological function. Modulates genomic RNA replication. Modulates subgenomic RNA synthesis by interacting with human C1QBP/SF2P32. Induces both perinuclear clustering of mitochondria and the formation of electron-dense intermitochondrial plaques, both hallmarks of rubella virus infected cells. Induces apoptosis when expressed in transfected cells. E2 envelope glycoprotein is responsible for viral attachment to target host cell, by binding to the cell receptor. Its transport to the plasma membrane depends on interaction with E1 protein. E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and clathrin-mediated endocytosis, acidification of the endosome would induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion (By similarity). E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Rubv~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Fokine, A~~]]	+	[[Category: Rubella virus strain M33]]
-	[[Category: ~~Prasad, V Mangala~~]]	+	[[Category: Fokine A]]
-	[[Category: ~~Rossmann, M G~~]]	+	[[Category: Mangala Prasad V]]
-	[[Category: ~~Capsid protein]]~~	+	[[Category: Rossmann MG]]
-	~~[[Category: Partial beta barrel]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 11:04, 4 August 2016

2016-08-04T11:04:19Z

←Older revision		Revision as of 11:04, 4 August 2016
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		+
	==Crystal Structure of Rubella virus capsid protein (residues 127-277)==		==Crystal Structure of Rubella virus capsid protein (residues 127-277)==
	<StructureSection load='4hbo' size='340' side='right' caption='[[4hbo]], [[Resolution\|resolution]] 3.24Å' scene=''>		<StructureSection load='4hbo' size='340' side='right' caption='[[4hbo]], [[Resolution\|resolution]] 3.24Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4har\|4har]], [[4hbe\|4hbe]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4har\|4har]], [[4hbe\|4hbe]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Capsid ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11043 RUBV])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Capsid ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11043 RUBV])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hbo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hbo RCSB], [http://www.ebi.ac.uk/pdbsum/4hbo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hbo OCA], [http://pdbe.org/4hbo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hbo RCSB], [http://www.ebi.ac.uk/pdbsum/4hbo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hbo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hbo" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:53, 21 December 2014

2014-12-21T17:53:58Z

←Older revision		Revision as of 17:53, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4hbo\| PDB=4hbo \| SCENE= }}~~	+	==Crystal Structure of Rubella virus capsid protein (residues 127-277)==
-	===Crystal Structure of Rubella virus capsid protein (residues 127-277)===	+	<StructureSection load='4hbo' size='340' side='right' caption='[[4hbo]], [[Resolution\|resolution]] 3.24Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24282305}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hbo]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Rubv Rubv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HBO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HBO FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4har\|4har]], [[4hbe\|4hbe]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Capsid ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11043 RUBV])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hbo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hbo RCSB], [http://www.ebi.ac.uk/pdbsum/4hbo PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rubella virus (RV) is a leading cause of birth defects due to infectious agents. When contracted during pregnancy, RV infection leads to severe damage in fetuses. Despite its medical importance, compared with the related alphaviruses, very little is known about the structure of RV. The RV capsid protein is an essential structural component of virions as well as a key factor in virus-host interactions. Here we describe three crystal structures of the structural domain of the RV capsid protein. The polypeptide fold of the RV capsid protomer has not been observed previously. Combining the atomic structure of the RV capsid protein with the cryoelectron tomograms of RV particles established a low-resolution structure of the virion. Mutational studies based on this structure confirmed the role of amino acid residues in the capsid that function in the assembly of infectious virions.

-	~~==About this Structure==~~	+	Rubella virus capsid protein structure and its role in virus assembly and infection.,Mangala Prasad V, Willows SD, Fokine A, Battisti AJ, Sun S, Plevka P, Hobman TC, Rossmann MG Proc Natl Acad Sci U S A. 2013 Nov 26. PMID:24282305<ref>PMID:24282305</ref>
-	~~[[4hbo]] is a 5 chain~~ structure. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4HBO OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024282305</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
-	[[Category: Fokine, A.]]	+	== References ==
-	[[Category: Prasad, V Mangala.]]	+	<references/>
-	[[Category: Rossmann, M G.]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Rubv]]
		+	[[Category: Fokine, A]]
		+	[[Category: Prasad, V Mangala]]
		+	[[Category: Rossmann, M G]]
	[[Category: Capsid protein]]		[[Category: Capsid protein]]
	[[Category: Partial beta barrel]]		[[Category: Partial beta barrel]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 13:38, 11 December 2013

2013-12-11T13:38:24Z

←Older revision		Revision as of 13:38, 11 December 2013
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4hbo\| PDB=4hbo \| SCENE= }}
		+	===Crystal Structure of Rubella virus capsid protein (residues 127-277)===
		+	{{ABSTRACT_PUBMED_24282305}}

-	~~The entry~~ 4hbo is ~~ON HOLD until May 27 2015~~	+	==About this Structure==
		+	[[4hbo]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HBO OCA].

-	~~Authors~~: ~~Mangala Prasad, V.,~~ Fokine, A., Rossmann, M.G.	+	==Reference==
-		+	<ref group="xtra">PMID:024282305</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal Structure of Rubella virus capsid~~ protein ~~(residues 127-277)~~	+	[[Category: Fokine, A.]]
		+	[[Category: Prasad, V Mangala.]]
		+	[[Category: Rossmann, M G.]]
		+	[[Category: Capsid protein]]
		+	[[Category: Partial beta barrel]]
		+	[[Category: Viral protein]]

OCA at 07:03, 14 August 2013

2013-08-14T07:03:34Z

←Older revision		Revision as of 07:03, 14 August 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hbo is ON HOLD until ~~Paper Publication~~	+	The entry 4hbo is ON HOLD until May 27 2015

	Authors: Mangala Prasad, V., Fokine, A., Rossmann, M.G.		Authors: Mangala Prasad, V., Fokine, A., Rossmann, M.G.

	Description: Crystal Structure of Rubella virus capsid protein (residues 127-277)		Description: Crystal Structure of Rubella virus capsid protein (residues 127-277)

OCA at 06:14, 14 November 2012

2012-11-14T06:14:35Z

←Older revision		Revision as of 06:14, 14 November 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hbo is ON HOLD	+	The entry 4hbo is ON HOLD until Paper Publication

	Authors: Mangala Prasad, V., Fokine, A., Rossmann, M.G.		Authors: Mangala Prasad, V., Fokine, A., Rossmann, M.G.

	Description: Crystal Structure of Rubella virus capsid protein (residues 127-277)		Description: Crystal Structure of Rubella virus capsid protein (residues 127-277)

OCA: Protected "4hbo" [edit=sysop:move=sysop]

2012-10-10T06:58:37Z

Protected "4hbo" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:58, 10 October 2012

OCA: New page: '''Unreleased structure''' The entry 4hbo is ON HOLD Authors: Mangala Prasad, V., Fokine, A., Rossmann, M.G. Description: Crystal Structure of Rubella virus capsid protein (residues 12...

2012-10-10T06:58:36Z

New page: '''Unreleased structure''' The entry 4hbo is ON HOLD Authors: Mangala Prasad, V., Fokine, A., Rossmann, M.G. Description: Crystal Structure of Rubella virus capsid protein (residues 12...

New page

'''Unreleased structure'''

The entry 4hbo is ON HOLD

Authors: Mangala Prasad, V., Fokine, A., Rossmann, M.G.

Description: Crystal Structure of Rubella virus capsid protein (residues 127-277)