4hfm - Revision history

OCA at 15:04, 20 September 2023

2023-09-20T15:04:55Z

←Older revision		Revision as of 15:04, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hfm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HFM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hfm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HFM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfm OCA], [https://pdbe.org/4hfm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hfm RCSB], [https://www.ebi.ac.uk/pdbsum/4hfm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hfm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfm OCA], [https://pdbe.org/4hfm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hfm RCSB], [https://www.ebi.ac.uk/pdbsum/4hfm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hfm ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:14, 3 November 2022

2022-11-03T08:14:00Z

←Older revision		Revision as of 08:14, 3 November 2022
Line 1:		Line 1:

	==X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum==		==X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum==
-	<StructureSection load='4hfm' size='340' side='right' caption='[[4hfm]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='4hfm' size='340' side='right'caption='[[4hfm]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hfm]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~American_tobacco American tobacco~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HFM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hfm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HFM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hfj\|4hfj]], [[4hfn\|4hfn]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfm OCA], [https://pdbe.org/4hfm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hfm RCSB], [https://www.ebi.ac.uk/pdbsum/4hfm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hfm ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NtADH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-alkenal_reductase_(NAD(P)(+)) 2-alkenal reductase (NAD(P)(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.74 1.3.1.74] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfm OCA], [~~http~~://pdbe.org/4hfm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hfm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hfm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hfm ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DBR_TOBAC DBR_TOBAC]] Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones (PubMed:17945329, Ref.3). May also act as a allyl-alcohol dehydrogenase by catalyzing the dehydrogenation of secondary allylic alcohols rather than saturated secondary alcohols. Allyl-alcohol dehydrogenase is specific for the S-stereoisomer of the alcohols (PubMed:11117876).<ref>PMID:11117876</ref> <ref>PMID:17945329</ref> [REFERENCE:3]	+	[https://www.uniprot.org/uniprot/DBR_TOBAC DBR_TOBAC] Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones (PubMed:17945329, Ref.3). May also act as a allyl-alcohol dehydrogenase by catalyzing the dehydrogenation of secondary allylic alcohols rather than saturated secondary alcohols. Allyl-alcohol dehydrogenase is specific for the S-stereoisomer of the alcohols (PubMed:11117876).<ref>PMID:11117876</ref> <ref>PMID:17945329</ref> [REFERENCE:3]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 20:

	==See Also==		==See Also==
-	*[[Alcohol dehydrogenase\|Alcohol dehydrogenase]]	+	*[[Alcohol dehydrogenase 3D structures\|Alcohol dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~American tobacco~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Scrutton, N S~~]]	+	[[Category: Nicotiana tabacum]]
-	[[Category: ~~Toogood, H S~~]]	+	[[Category: Scrutton NS]]
-	[[Category: ~~Alkene reduction]]~~	+	[[Category: Toogood HS]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Twisted b-barrel~~]]	+

OCA at 06:07, 18 April 2018

2018-04-18T06:07:48Z

←Older revision		Revision as of 06:07, 18 April 2018
Line 12:		Line 12:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/DBR_TOBAC DBR_TOBAC]] Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones (PubMed:17945329, Ref.3). May also act as a allyl-alcohol dehydrogenase by catalyzing the dehydrogenation of secondary allylic alcohols rather than saturated secondary alcohols. Allyl-alcohol dehydrogenase is specific for the S-stereoisomer of the alcohols (PubMed:11117876).<ref>PMID:11117876</ref> <ref>PMID:17945329</ref> [REFERENCE:3]		[[http://www.uniprot.org/uniprot/DBR_TOBAC DBR_TOBAC]] Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones (PubMed:17945329, Ref.3). May also act as a allyl-alcohol dehydrogenase by catalyzing the dehydrogenation of secondary allylic alcohols rather than saturated secondary alcohols. Allyl-alcohol dehydrogenase is specific for the S-stereoisomer of the alcohols (PubMed:11117876).<ref>PMID:11117876</ref> <ref>PMID:17945329</ref> [REFERENCE:3]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The application of biocatalysis for the asymmetric reduction of activated C=C is a powerful tool for the manufacture of high-value chemical commodities. The biocatalytic potential of "-ene" reductases from the Old Yellow Enzyme (OYE) family of oxidoreductases is well-known; however, the specificity of these enzymes toward mainly small molecule substrates has highlighted the need to discover "-ene" reductases from different enzymatic classes to broaden industrial applicability. Here, we describe the characterization of a flavin-free double bond reductase from Nicotiana tabacum (NtDBR), which belongs to the leukotriene B4 dehydrogenase (LTD) subfamily of the zinc-independent, medium chain dehydrogenase/reductase superfamily of enzymes. Using steady-state kinetics and biotransformation reactions, we have demonstrated the regio- and stereospecificity of NtDBR against a variety of alpha,beta-unsaturated activated alkenes. In addition to catalyzing the reduction of typical LTD substrates and several classical OYE-like substrates, NtDBR also exhibited complementary activity by reducing non-OYE substrates (i.e., reducing the exocyclic C=C double bond of (R)-pulegone) and in some cases showing an opposite stereopreference in comparison with the OYE family member pentaerythritol tetranitrate (PETN) reductase. This serves to augment classical OYE "-ene" reductase activity and, coupled with its aerobic stability, emphasizes the potential industrial value of NtDBR. Furthermore, we also report the X-ray crystal structures of the holo-, binary NADP(H)-bound, and ternary [NADP(+) and 4-hydroxy-3-methoxycinnamaldehyde (9a)-bound] NtDBR complexes. These will underpin structure-driven site-saturated mutagenesis studies aimed at enhancing the reactivity, stereochemistry, and specificity of this enzyme.
		+
		+	Biocatalytic Asymmetric Alkene Reduction: Crystal Structure and Characterization of a Double Bond Reductase from Nicotiana tabacum.,Mansell DJ, Toogood HS, Waller J, Hughes JM, Levy CW, Gardiner JM, Scrutton NS ACS Catal. 2013 Mar 1;3(3):370-379. doi: 10.1021/cs300709m. Epub 2013 Jan 21. PMID:27547488<ref>PMID:27547488</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4hfm" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 21:24, 11 August 2016

2016-08-11T21:24:37Z

←Older revision		Revision as of 21:24, 11 August 2016
Line 1:		Line 1:
		+
	==X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum==		==X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum==
	<StructureSection load='4hfm' size='340' side='right' caption='[[4hfm]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4hfm' size='340' side='right' caption='[[4hfm]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hfm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Nicotiana_tabacum Nicotiana tabacum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HFM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hfm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HFM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hfj\|4hfj]], [[4hfn\|4hfn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hfj\|4hfj]], [[4hfn\|4hfn]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NtADH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 ~~Nicotiana tabacum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NtADH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-~~alkenal_reductase~~ 2-alkenal reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.74 1.3.1.74] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-alkenal_reductase_(NAD(P)(+)) 2-alkenal reductase (NAD(P)(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.74 1.3.1.74] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hfm RCSB], [http://www.ebi.ac.uk/pdbsum/4hfm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfm OCA], [http://pdbe.org/4hfm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hfm RCSB], [http://www.ebi.ac.uk/pdbsum/4hfm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hfm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q9SLN8_TOBAC Q9SLN8_TOBAC~~]] Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones (PubMed:17945329, Ref.3). May also act as a allyl-alcohol dehydrogenase by catalyzing the dehydrogenation of secondary allylic alcohols rather than saturated secondary alcohols. Allyl-alcohol dehydrogenase is specific for the S-stereoisomer of the alcohols (PubMed:11117876).<ref>PMID:11117876</ref> <ref>PMID:17945329</ref> [REFERENCE:3]	+	[[http://www.uniprot.org/uniprot/DBR_TOBAC DBR_TOBAC]] Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones (PubMed:17945329, Ref.3). May also act as a allyl-alcohol dehydrogenase by catalyzing the dehydrogenation of secondary allylic alcohols rather than saturated secondary alcohols. Allyl-alcohol dehydrogenase is specific for the S-stereoisomer of the alcohols (PubMed:11117876).<ref>PMID:11117876</ref> <ref>PMID:17945329</ref> [REFERENCE:3]

	==See Also==		==See Also==
Line 18:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~2-alkenal reductase]]~~	+	[[Category: American tobacco]]
-	~~[[Category: Nicotiana tabacum~~]]	+
	[[Category: Scrutton, N S]]		[[Category: Scrutton, N S]]
	[[Category: Toogood, H S]]		[[Category: Toogood, H S]]

OCA at 11:54, 20 January 2015

2015-01-20T11:54:27Z

←Older revision		Revision as of 11:54, 20 January 2015
Line 1:		Line 1:
-	[[~~Image:~~4hfm.~~jpg~~\|~~left~~\|~~200px~~]]	+	==X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum==
		+	<StructureSection load='4hfm' size='340' side='right' caption='[[4hfm]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hfm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HFM FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hfj\|4hfj]], [[4hfn\|4hfn]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NtADH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 Nicotiana tabacum])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-alkenal_reductase 2-alkenal reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.74 1.3.1.74] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hfm RCSB], [http://www.ebi.ac.uk/pdbsum/4hfm PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q9SLN8_TOBAC Q9SLN8_TOBAC]] Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones (PubMed:17945329, Ref.3). May also act as a allyl-alcohol dehydrogenase by catalyzing the dehydrogenation of secondary allylic alcohols rather than saturated secondary alcohols. Allyl-alcohol dehydrogenase is specific for the S-stereoisomer of the alcohols (PubMed:11117876).<ref>PMID:11117876</ref> <ref>PMID:17945329</ref> [REFERENCE:3]

-	~~{{STRUCTURE_4hfm\| PDB~~=~~4hfm \| SCENE= }}~~	+	==See Also==
-		+	*[[Alcohol dehydrogenase\|Alcohol dehydrogenase]]
-	~~===X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum~~===	+	== References ==
-		+	<references/>
-		+	__TOC__
-	==~~About this Structure~~==	+	</StructureSection>
-	~~[[4hfm]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFM OCA].~~	+
	[[Category: 2-alkenal reductase]]		[[Category: 2-alkenal reductase]]
	[[Category: Nicotiana tabacum]]		[[Category: Nicotiana tabacum]]
-	[[Category: Scrutton, N S.]]	+	[[Category: Scrutton, N S]]
-	[[Category: Toogood, H S.]]	+	[[Category: Toogood, H S]]
	[[Category: Alkene reduction]]		[[Category: Alkene reduction]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: Twisted b-barrel]]		[[Category: Twisted b-barrel]]

OCA at 12:40, 30 January 2013

2013-01-30T12:40:17Z

←Older revision		Revision as of 12:40, 30 January 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4hfm.jpg\|left\|200px]]

-	~~The entry~~ 4hfm ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4hfm\| PDB=4hfm \| SCENE= }}

-	~~Authors: Toogood,~~ H~~.S., Scrutton, N.S.~~	+	===X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum===

-	~~Description: X-ray Crystal~~ Structure of a ~~NADP(H)-bound Double Bond Reductase~~ from Nicotiana tabacum	+
		+	==About this Structure==
		+	[[4hfm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFM OCA].
		+	[[Category: 2-alkenal reductase]]
		+	[[Category: Nicotiana tabacum]]
		+	[[Category: Scrutton, N S.]]
		+	[[Category: Toogood, H S.]]
		+	[[Category: Alkene reduction]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Twisted b-barrel]]

OCA at 08:38, 31 October 2012

2012-10-31T08:38:38Z

←Older revision		Revision as of 08:38, 31 October 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hfm is ON HOLD	+	The entry 4hfm is ON HOLD until Paper Publication

	Authors: Toogood, H.S., Scrutton, N.S.		Authors: Toogood, H.S., Scrutton, N.S.

	Description: X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum		Description: X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum

OCA: Protected "4hfm" [edit=sysop:move=sysop]

2012-10-17T06:36:05Z

Protected "4hfm" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:36, 17 October 2012

OCA: New page: '''Unreleased structure''' The entry 4hfm is ON HOLD Authors: Toogood, H.S., Scrutton, N.S. Description: X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana...

2012-10-17T06:36:04Z

New page: '''Unreleased structure''' The entry 4hfm is ON HOLD Authors: Toogood, H.S., Scrutton, N.S. Description: X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana...

New page

'''Unreleased structure'''

The entry 4hfm is ON HOLD

Authors: Toogood, H.S., Scrutton, N.S.

Description: X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum