4hvk - Revision history

OCA at 11:45, 1 March 2024

2024-03-01T11:45:02Z

←Older revision		Revision as of 11:45, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HVK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HVK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.43Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvk OCA], [https://pdbe.org/4hvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hvk RCSB], [https://www.ebi.ac.uk/pdbsum/4hvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hvk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvk OCA], [https://pdbe.org/4hvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hvk RCSB], [https://www.ebi.ac.uk/pdbsum/4hvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hvk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ISCS2_ARCFU ISCS2_ARCFU] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).		[https://www.uniprot.org/uniprot/ISCS2_ARCFU ISCS2_ARCFU] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	l-Cysteine desulfurase IscS and scaffold IscU proteins are universally involved in Fe/S cluster synthesis. The Archaeoglobus fulgidus (Af) genome encodes proteins having a high degree of primary structure similarity to IscS and IscU from other organisms. However, AfIscS is unusual because it lacks the active site lysine residue that normally forms an internal Schiff base with pyridoxal-phosphate (PLP) and serves as a base during catalysis. Our as-isolated recombinant AfIscS contains pyridoxamine phosphate (PMP) instead of the expected PLP and lacks desulfurase activity. We have solved its structure to 1.43 A resolution and found that PMP binds non-covalently at the PLP site of the enzyme and displays significant disorder. However, the previously reported structure of recombinant Af(IscU-D35A-IscS)(2) contains an in vivo generated [Fe(2)S(2)] species within AfIscU and the question arises as to how its sulfides were generated. Here, we report that adding PLP to AfIscS produces an enzyme that displays in vitrol-cysteine desulfurase activity mediating the synthesis of a stable holo Af(IscU-D35A-IscS) complex.
-
-	Crystal structure and functional studies of an unusual l-cysteine desulfurase from Archaeoglobus fulgidus.,Yamanaka Y, Zeppieri L, Nicolet Y, Marinoni EN, de Oliveira JS, Odaka M, Dean DR, Fontecilla-Camps JC Dalton Trans. 2012 Nov 19. PMID:23160436<ref>PMID:23160436</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4hvk" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:18, 9 November 2022

2022-11-09T08:18:19Z

←Older revision		Revision as of 08:18, 9 November 2022
Line 1:		Line 1:

	==Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.==		==Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.==
-	<StructureSection load='4hvk' size='340' side='right' caption='[[4hvk]], [[Resolution\|resolution]] 1.43Å' scene=''>	+	<StructureSection load='4hvk' size='340' side='right'caption='[[4hvk]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hvk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arcfu Arcfu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HVK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HVK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eb5\|4eb5]], [[4eb7\|4eb7]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvk OCA], [https://pdbe.org/4hvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hvk RCSB], [https://www.ebi.ac.uk/pdbsum/4hvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hvk ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF0564, AF_0564, iscS2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvk OCA], [~~http~~://pdbe.org/4hvk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hvk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hvk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hvk ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ISCS2_ARCFU ISCS2_ARCFU]] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).	+	[https://www.uniprot.org/uniprot/ISCS2_ARCFU ISCS2_ARCFU] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arcfu~~]]	+	[[Category: Archaeoglobus fulgidus DSM 4304]]
-	[[Category: ~~Cysteine desulfurase~~]]	+	[[Category: Large Structures]]
-	[[Category: Dean~~, D R~~]]	+	[[Category: Dean DR]]
-	[[Category: Fontecilla-Camps~~, J C~~]]	+	[[Category: Fontecilla-Camps JC]]
-	[[Category: Marinoni~~, E N~~]]	+	[[Category: Marinoni EN]]
-	[[Category: Masafumi, O]]	+	[[Category: Masafumi O]]
-	[[Category: Nicolet, Y]]	+	[[Category: Nicolet Y]]
-	~~[[Category: Oliveira, J S.de~~]]	+	[[Category: Yamanaka Y]]
-	[[Category: Yamanaka, Y]]	+	[[Category: Zeppieri L]]
-	[[Category: Zeppieri, L]]	+	[[Category: De Oliveira JS]]
-	[[Category: ~~Transferase]]~~	+
-	~~[[Category: Transferase and isc~~]]	+

OCA at 02:00, 5 August 2016

2016-08-05T02:00:05Z

←Older revision		Revision as of 02:00, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.==		==Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.==
	<StructureSection load='4hvk' size='340' side='right' caption='[[4hvk]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='4hvk' size='340' side='right' caption='[[4hvk]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HVK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfu Arcfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HVK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eb5\|4eb5]], [[4eb7\|4eb7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eb5\|4eb5]], [[4eb7\|4eb7]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF0564, AF_0564, iscS2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ~~Archaeoglobus fulgidus DSM 4304~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF0564, AF_0564, iscS2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hvk RCSB], [http://www.ebi.ac.uk/pdbsum/4hvk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvk OCA], [http://pdbe.org/4hvk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hvk RCSB], [http://www.ebi.ac.uk/pdbsum/4hvk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hvk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hvk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Archaeoglobus fulgidus dsm 4304~~]]	+	[[Category: Arcfu]]
	[[Category: Cysteine desulfurase]]		[[Category: Cysteine desulfurase]]
	[[Category: Dean, D R]]		[[Category: Dean, D R]]

OCA at 14:53, 25 December 2014

2014-12-25T14:53:51Z

←Older revision		Revision as of 14:53, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hvk RCSB], [http://www.ebi.ac.uk/pdbsum/4hvk PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hvk RCSB], [http://www.ebi.ac.uk/pdbsum/4hvk PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ISCS2_ARCFU ISCS2_ARCFU]] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:47, 21 December 2014

2014-12-21T12:47:27Z

←Older revision		Revision as of 12:47, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4hvk\| PDB=4hvk \| SCENE= }}~~	+	==Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.==
-	===Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.===	+	<StructureSection load='4hvk' size='340' side='right' caption='[[4hvk]], [[Resolution\|resolution]] 1.43Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23160436}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HVK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4eb5\|4eb5]], [[4eb7\|4eb7]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF0564, AF_0564, iscS2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 Archaeoglobus fulgidus DSM 4304])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_desulfurase Cysteine desulfurase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.7 2.8.1.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hvk RCSB], [http://www.ebi.ac.uk/pdbsum/4hvk PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	l-Cysteine desulfurase IscS and scaffold IscU proteins are universally involved in Fe/S cluster synthesis. The Archaeoglobus fulgidus (Af) genome encodes proteins having a high degree of primary structure similarity to IscS and IscU from other organisms. However, AfIscS is unusual because it lacks the active site lysine residue that normally forms an internal Schiff base with pyridoxal-phosphate (PLP) and serves as a base during catalysis. Our as-isolated recombinant AfIscS contains pyridoxamine phosphate (PMP) instead of the expected PLP and lacks desulfurase activity. We have solved its structure to 1.43 A resolution and found that PMP binds non-covalently at the PLP site of the enzyme and displays significant disorder. However, the previously reported structure of recombinant Af(IscU-D35A-IscS)(2) contains an in vivo generated [Fe(2)S(2)] species within AfIscU and the question arises as to how its sulfides were generated. Here, we report that adding PLP to AfIscS produces an enzyme that displays in vitrol-cysteine desulfurase activity mediating the synthesis of a stable holo Af(IscU-D35A-IscS) complex.

-	~~==Function==~~	+	Crystal structure and functional studies of an unusual l-cysteine desulfurase from Archaeoglobus fulgidus.,Yamanaka Y, Zeppieri L, Nicolet Y, Marinoni EN, de Oliveira JS, Odaka M, Dean DR, Fontecilla-Camps JC Dalton Trans. 2012 Nov 19. PMID:23160436<ref>PMID:23160436</ref>
-	~~[[http://www~~.~~uniprot~~.~~org~~/~~uniprot/ISCS2_ARCFU ISCS2_ARCFU]] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).~~	+

-	==~~About this Structure~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4hvk]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVK OCA].~~	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Archaeoglobus fulgidus dsm 4304]]		[[Category: Archaeoglobus fulgidus dsm 4304]]
	[[Category: Cysteine desulfurase]]		[[Category: Cysteine desulfurase]]
-	[[Category: Dean, D R.]]	+	[[Category: Dean, D R]]
-	[[Category: Fontecilla-Camps, J C.]]	+	[[Category: Fontecilla-Camps, J C]]
-	[[Category: Marinoni, E N.]]	+	[[Category: Marinoni, E N]]
-	[[Category: Masafumi, O.]]	+	[[Category: Masafumi, O]]
-	[[Category: Nicolet, Y.]]	+	[[Category: Nicolet, Y]]
-	[[Category: Oliveira, J S.de.]]	+	[[Category: Oliveira, J S.de]]
-	[[Category: Yamanaka, Y.]]	+	[[Category: Yamanaka, Y]]
-	[[Category: Zeppieri, L.]]	+	[[Category: Zeppieri, L]]
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Transferase and isc]]		[[Category: Transferase and isc]]

OCA at 10:13, 24 April 2013

2013-04-24T10:13:15Z

←Older revision		Revision as of 10:13, 24 April 2013
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-	[[Image:4hvk.jpg\|left\|200px]]
-
	{{STRUCTURE_4hvk\| PDB=4hvk \| SCENE= }}		{{STRUCTURE_4hvk\| PDB=4hvk \| SCENE= }}
-
	===Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.===		===Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.===
		+	{{ABSTRACT_PUBMED_23160436}}

-	~~{{ABSTRACT_PUBMED_23160436}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/ISCS2_ARCFU ISCS2_ARCFU]] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).

	==About this Structure==		==About this Structure==

OCA at 10:51, 5 December 2012

2012-12-05T10:51:30Z

←Older revision		Revision as of 10:51, 5 December 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:4hvk.jpg\|left\|200px]]

-	~~The entry~~ 4hvk ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4hvk\| PDB=4hvk \| SCENE= }}

-	~~Authors: Yamanaka, Y., Zeppieri,~~ L~~., Nicolet, Y., Marinoni, E.N., de Oliveira, J.S., Masafumi, O., Dean, D.R., Fontecilla~~-~~Camps, J.C~~.	+	===Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.===

-	~~Description: Crystal~~ structure ~~and functional studies of an unusual L-cysteine desulfurase~~ from Archaeoglobus fulgidus.	+	{{ABSTRACT_PUBMED_23160436}}
		+
		+	==About this Structure==
		+	[[4hvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVK OCA].
		+	[[Category: Archaeoglobus fulgidus dsm 4304]]
		+	[[Category: Cysteine desulfurase]]
		+	[[Category: Dean, D R.]]
		+	[[Category: Fontecilla-Camps, J C.]]
		+	[[Category: Marinoni, E N.]]
		+	[[Category: Masafumi, O.]]
		+	[[Category: Nicolet, Y.]]
		+	[[Category: Oliveira, J S.de.]]
		+	[[Category: Yamanaka, Y.]]
		+	[[Category: Zeppieri, L.]]
		+	[[Category: Transferase]]
		+	[[Category: Transferase and isc]]

OCA: Protected "4hvk" [edit=sysop:move=sysop]

2012-11-14T06:20:11Z

Protected "4hvk" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:20, 14 November 2012

OCA: New page: '''Unreleased structure''' The entry 4hvk is ON HOLD until Paper Publication Authors: Yamanaka, Y., Zeppieri, L., Nicolet, Y., Marinoni, E.N., de Oliveira, J.S., Masafumi, O., Dean, D.R...

2012-11-14T06:20:10Z

New page: '''Unreleased structure''' The entry 4hvk is ON HOLD until Paper Publication Authors: Yamanaka, Y., Zeppieri, L., Nicolet, Y., Marinoni, E.N., de Oliveira, J.S., Masafumi, O., Dean, D.R...

New page

'''Unreleased structure'''

The entry 4hvk is ON HOLD until Paper Publication

Authors: Yamanaka, Y., Zeppieri, L., Nicolet, Y., Marinoni, E.N., de Oliveira, J.S., Masafumi, O., Dean, D.R., Fontecilla-Camps, J.C.

Description: Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus.