4hzc - Revision history

OCA at 08:29, 9 November 2022

2022-11-09T08:29:19Z

←Older revision		Revision as of 08:29, 9 November 2022
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	==Crystal structure of Serine acetyltransferase from Brucella abortus strain S19==		==Crystal structure of Serine acetyltransferase from Brucella abortus strain S19==
-	<StructureSection load='4hzc' size='340' side='right' caption='[[4hzc]], [[Resolution\|resolution]] 1.97Å' scene=''>	+	<StructureSection load='4hzc' size='340' side='right'caption='[[4hzc]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hzc]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Brua1 Brua1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HZC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hzc]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus_S19 Brucella abortus S19]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HZC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hzd\|4hzd]], [[3mc4\|3mc4]], [[3p1b\|3p1b]], [[1ssq\|1ssq]], [[1t3d\|1t3d]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzc OCA], [https://pdbe.org/4hzc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hzc RCSB], [https://www.ebi.ac.uk/pdbsum/4hzc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hzc ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAbS19_I11940, CysE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=430066 BRUA1])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzc OCA], [~~http~~://pdbe.org/4hzc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hzc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hzc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hzc ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0F6AR69_BRUA1 A0A0F6AR69_BRUA1]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4hzc" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4hzc" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Serine acetyltransferase\|Serine acetyltransferase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Brua1~~]]	+	[[Category: Brucella abortus S19]]
-	[[Category: ~~Kumar, S~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Samudrala, G~~]]	+	[[Category: Kumar S]]
-	[[Category: ~~Acetyltransferase]]~~	+	[[Category: Samudrala G]]
-	~~[[Category: Cysteine biosynthesis]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 12:38, 11 August 2016

2016-08-11T12:38:09Z

←Older revision		Revision as of 12:38, 11 August 2016
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		+
	==Crystal structure of Serine acetyltransferase from Brucella abortus strain S19==		==Crystal structure of Serine acetyltransferase from Brucella abortus strain S19==
	<StructureSection load='4hzc' size='340' side='right' caption='[[4hzc]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='4hzc' size='340' side='right' caption='[[4hzc]], [[Resolution\|resolution]] 1.97Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hzd\|4hzd]], [[3mc4\|3mc4]], [[3p1b\|3p1b]], [[1ssq\|1ssq]], [[1t3d\|1t3d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hzd\|4hzd]], [[3mc4\|3mc4]], [[3p1b\|3p1b]], [[1ssq\|1ssq]], [[1t3d\|1t3d]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAbS19_I11940, CysE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=430066 BRUA1])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAbS19_I11940, CysE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=430066 BRUA1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hzc RCSB], [http://www.ebi.ac.uk/pdbsum/4hzc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzc OCA], [http://pdbe.org/4hzc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hzc RCSB], [http://www.ebi.ac.uk/pdbsum/4hzc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hzc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hzc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:24, 5 January 2015

2015-01-05T14:24:13Z

←Older revision		Revision as of 14:24, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hzc]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Brua1 Brua1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HZC FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hzc]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Brua1 Brua1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HZC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hzd\|4hzd]], [[3mc4\|3mc4]], [[3p1b\|3p1b]], [[1ssq\|1ssq]], [[1t3d\|1t3d]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hzd\|4hzd]], [[3mc4\|3mc4]], [[3p1b\|3p1b]], [[1ssq\|1ssq]], [[1t3d\|1t3d]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAbS19_I11940, CysE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=430066 BRUA1])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAbS19_I11940, CysE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=430066 BRUA1])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hzc RCSB], [http://www.ebi.ac.uk/pdbsum/4hzc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hzc RCSB], [http://www.ebi.ac.uk/pdbsum/4hzc PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Brua1]]		[[Category: Brua1]]
-	[[Category: Kumar, S.]]	+	[[Category: Kumar, S]]
-	[[Category: Samudrala, G.]]	+	[[Category: Samudrala, G]]
	[[Category: Acetyltransferase]]		[[Category: Acetyltransferase]]
	[[Category: Cysteine biosynthesis]]		[[Category: Cysteine biosynthesis]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 02:20, 7 August 2014

2014-08-07T02:20:17Z

←Older revision		Revision as of 02:20, 7 August 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4hzc\| PDB=4hzc \| SCENE= }}~~	+	==Crystal structure of Serine acetyltransferase from Brucella abortus strain S19==
-	===Crystal structure of Serine acetyltransferase from Brucella abortus strain S19===	+	<StructureSection load='4hzc' size='340' side='right' caption='[[4hzc]], [[Resolution\|resolution]] 1.97Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hzc]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Brua1 Brua1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HZC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hzd\|4hzd]], [[3mc4\|3mc4]], [[3p1b\|3p1b]], [[1ssq\|1ssq]], [[1t3d\|1t3d]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAbS19_I11940, CysE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=430066 BRUA1])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hzc RCSB], [http://www.ebi.ac.uk/pdbsum/4hzc PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Brucella abortus is the major cause of premature foetal abortion in cattle, can be transmitted from cattle to humans, and is considered a powerful biological weapon. De novo cysteine biosynthesis is one of the essential pathways reported in bacteria, protozoa, and plants. Serine acetyltransferase (SAT) initiates this reaction by catalyzing the formation of O-acetylserine (OAS) using l-serine and acetyl coenzyme A as substrates. Here we report kinetic and crystallographic studies of this enzyme from B. abortus. The kinetic studies indicate that cysteine competitively inhibits the binding of serine to B. abortus SAT (BaSAT) and noncompetitively inhibits the binding of acetyl coenzyme A. The crystal structures of BaSAT in its apo state and in complex with coenzyme A (CoA) were determined to 1.96A and 1.87A resolution, respectively. BaSAT was observed as a trimer in a size exclusion column; however, it was seen as a hexamer in dynamic light scattering (DLS) studies and in the crystal structure, indicating it may exist in both states. The complex structure shows coenzyme A bound to the C-terminal region, making mostly hydrophobic contacts from the center of the active site extending up to the surface of the protein. There is no conformational difference in the enzyme between the apo and the complexed states, indicating lock and key binding and the absence of an induced fit mechanism.

-	~~==About this Structure==~~	+	Crystal structure of serine acetyl transferase from Brucella abortus and its complex with coenzyme A.,Kumar S, Kumar N, Alam N, Gourinath S Biochim Biophys Acta. 2014 Jul 22;1844(10):1741-1748. doi:, 10.1016/j.bbapap.2014.07.009. PMID:25058332<ref>PMID:25058332</ref>
-	~~[[4hzc]] is a 12 chain~~ structure~~. Full crystallographic information is available~~ from ~~[http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin~~/~~ocashort?id~~=~~4HZC OCA~~].	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Brua1]]
	[[Category: Kumar, S.]]		[[Category: Kumar, S.]]
	[[Category: Samudrala, G.]]		[[Category: Samudrala, G.]]

OCA at 17:45, 19 February 2014

2014-02-19T17:45:09Z

←Older revision		Revision as of 17:45, 19 February 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4hzc\| PDB=4hzc \| SCENE= }}
		+	===Crystal structure of Serine acetyltransferase from Brucella abortus strain S19===

-	~~The entry~~ 4hzc is ~~ON HOLD until Feb 14 2015~~	+	==About this Structure==
-		+	[[4hzc]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZC OCA].
-	~~Authors~~: Kumar, S., Samudrala, G.	+	[[Category: Kumar, S.]]
-		+	[[Category: Samudrala, G.]]
-	~~Description~~: ~~Crystal structure of Serine acetyltransferase from Brucella abortus strain S19~~	+	[[Category: Acetyltransferase]]
		+	[[Category: Cysteine biosynthesis]]
		+	[[Category: Transferase]]

OCA at 06:42, 6 November 2013

2013-11-06T06:42:46Z

←Older revision		Revision as of 06:42, 6 November 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hzc is ON HOLD until ~~Paper Publication~~	+	The entry 4hzc is ON HOLD until Feb 14 2015

	Authors: Kumar, S., Samudrala, G.		Authors: Kumar, S., Samudrala, G.

	Description: Crystal structure of Serine acetyltransferase from Brucella abortus strain S19		Description: Crystal structure of Serine acetyltransferase from Brucella abortus strain S19

OCA at 07:10, 8 May 2013

2013-05-08T07:10:48Z

←Older revision		Revision as of 07:10, 8 May 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hzc is ON HOLD	+	The entry 4hzc is ON HOLD until Paper Publication

	Authors: Kumar, S., Samudrala, G.		Authors: Kumar, S., Samudrala, G.

	Description: Crystal structure of Serine acetyltransferase from Brucella abortus strain S19		Description: Crystal structure of Serine acetyltransferase from Brucella abortus strain S19

OCA at 07:01, 2 May 2013

2013-05-02T07:01:37Z

←Older revision		Revision as of 07:01, 2 May 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hzc is ON HOLD ~~until Paper Publication~~	+	The entry 4hzc is ON HOLD

	Authors: Kumar, S., Samudrala, G.		Authors: Kumar, S., Samudrala, G.

	Description: Crystal structure of Serine acetyltransferase from Brucella abortus strain S19		Description: Crystal structure of Serine acetyltransferase from Brucella abortus strain S19

OCA at 10:02, 13 February 2013

2013-02-13T10:02:17Z

←Older revision		Revision as of 10:02, 13 February 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hzc is ON HOLD	+	The entry 4hzc is ON HOLD until Paper Publication

	Authors: Kumar, S., Samudrala, G.		Authors: Kumar, S., Samudrala, G.

	Description: Crystal structure of Serine acetyltransferase from Brucella abortus strain S19		Description: Crystal structure of Serine acetyltransferase from Brucella abortus strain S19

OCA at 10:30, 5 December 2012

2012-12-05T10:30:46Z

←Older revision		Revision as of 10:30, 5 December 2012
Line 3:		Line 3:
	The entry 4hzc is ON HOLD		The entry 4hzc is ON HOLD

-	Authors: Kumar,S., Samudrala, G.	+	Authors: Kumar, S., Samudrala, G.

-	Description: Crystal structure of Serine acetyltransferase from Brucella abortus strain S19.	+	Description: Crystal structure of Serine acetyltransferase from Brucella abortus strain S19