4i59 - Revision history

OCA at 15:15, 20 September 2023

2023-09-20T15:15:17Z

←Older revision		Revision as of 15:15, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4i59]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbacterium_oxydans Microbacterium oxydans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I59 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4i59]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbacterium_oxydans Microbacterium oxydans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I59 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYH:CYCLOHEXANONE'>CYH</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.93Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYH:CYCLOHEXANONE'>CYH</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i59 OCA], [https://pdbe.org/4i59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i59 RCSB], [https://www.ebi.ac.uk/pdbsum/4i59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i59 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i59 OCA], [https://pdbe.org/4i59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i59 RCSB], [https://www.ebi.ac.uk/pdbsum/4i59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i59 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:42, 9 November 2022

2022-11-09T08:42:27Z

←Older revision		Revision as of 08:42, 9 November 2022
Line 1:		Line 1:

	==Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone==		==Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone==
-	<StructureSection load='4i59' size='340' side='right' caption='[[4i59]], [[Resolution\|resolution]] 2.93Å' scene=''>	+	<StructureSection load='4i59' size='340' side='right'caption='[[4i59]], [[Resolution\|resolution]] 2.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4i59]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Brevibacterium_oxydans Brevibacterium~~ oxydans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I59 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4I59 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4i59]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbacterium_oxydans Microbacterium oxydans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I59 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYH:CYCLOHEXANONE'>CYH</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYH:CYCLOHEXANONE'>CYH</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4i58\|4i58]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i59 OCA], [https://pdbe.org/4i59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i59 RCSB], [https://www.ebi.ac.uk/pdbsum/4i59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i59 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82380 Brevibacterium oxydans])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4i59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i59 OCA], [~~http~~://pdbe.org/4i59 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4i59 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4i59 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4i59 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/R4GRV2_9MICO R4GRV2_9MICO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Brevibacterium oxydans~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Berghuis, A M~~]]	+	[[Category: Microbacterium oxydans]]
-	[[Category: ~~Mirza, I A~~]]	+	[[Category: Berghuis AM]]
-	[[Category: ~~Biocatalysis]]~~	+	[[Category: Mirza IA]]
-	~~[[Category: Cyclohexylamine oxidase]]~~	+
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Monoamine oxidase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:19, 4 August 2016

2016-08-04T07:19:58Z

←Older revision		Revision as of 07:19, 4 August 2016
Line 1:		Line 1:
		+
	==Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone==		==Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone==
	<StructureSection load='4i59' size='340' side='right' caption='[[4i59]], [[Resolution\|resolution]] 2.93Å' scene=''>		<StructureSection load='4i59' size='340' side='right' caption='[[4i59]], [[Resolution\|resolution]] 2.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4i59]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Microbacterium_oxydans Microbacterium~~ oxydans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I59 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4i59]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevibacterium_oxydans Brevibacterium oxydans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I59 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYH:CYCLOHEXANONE'>CYH</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYH:CYCLOHEXANONE'>CYH</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4i58\|4i58]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4i58\|4i58]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82380 ~~Microbacterium~~ oxydans])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82380 Brevibacterium oxydans])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i59 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4i59 RCSB], [http://www.ebi.ac.uk/pdbsum/4i59 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i59 OCA], [http://pdbe.org/4i59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4i59 RCSB], [http://www.ebi.ac.uk/pdbsum/4i59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4i59 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4i59" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Microbacterium~~ oxydans]]	+	[[Category: Brevibacterium oxydans]]
	[[Category: Berghuis, A M]]		[[Category: Berghuis, A M]]
	[[Category: Mirza, I A]]		[[Category: Mirza, I A]]

OCA at 12:08, 21 December 2014

2014-12-21T12:08:03Z

←Older revision		Revision as of 12:08, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4i59\| PDB=4i59 \| SCENE= }}~~	+	==Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone==
-	===Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone===	+	<StructureSection load='4i59' size='340' side='right' caption='[[4i59]], [[Resolution\|resolution]] 2.93Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23555888}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4i59]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Microbacterium_oxydans Microbacterium oxydans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I59 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYH:CYCLOHEXANONE'>CYH</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4i58\|4i58]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82380 Microbacterium oxydans])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i59 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4i59 RCSB], [http://www.ebi.ac.uk/pdbsum/4i59 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cyclohexylamine oxidase (CHAO) is a flavoprotein first described in Brevibacterium oxydans strain IH-35A that carries out the initial step of the degradation of the industrial chemical cyclohexylamine to cyclohexanone. We have cloned and expressed in Escherichia coli the CHAO-encoding gene (chaA) from B. oxydans, purified CHAO and determined the structures of both the holoenzyme form of the enzyme and a product complex with cyclohexanone. CHAO is a 50 kDa monomer with a PHBH fold topology. It belongs to the flavin monooxygenase family of enzymes and exhibits high substrate specificity for alicyclic amines and sec-alkylamines. The overall structure is similar to that of other members of the flavin monooxygenase family, but lacks either of the C- or N-terminal extensions observed in these enzymes. Active site features of the flavin monooxygenase family are conserved in CHAO, including the characteristic aromatic cage. Differences in the orientations of residues of the CHAO aromatic cage result in a substrate-binding site that is more open than those of its structural relatives. Since CHAO has a buried hydrophobic active site with no obvious route for substrates and products, a random acceleration molecular dynamics simulation has been used to identify a potential egress route. The path identified includes an intermediate cavity and requires transient conformation changes in a shielding loop and a residue at the border of the substrate-binding cavity. These results provide a foundation for further studies with CHAO aimed at identifying features determining substrate specificity and for developing the biocatalytic potential of this enzyme.

-	~~==About this Structure==~~	+	Structural Analysis of a Novel Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A.,Mirza IA, Burk DL, Xiong B, Iwaki H, Hasegawa Y, Grosse S, Lau PC, Berghuis AM PLoS One. 2013;8(3):e60072. doi: 10.1371/journal.pone.0060072. Epub 2013 Mar 26. PMID:23555888<ref>PMID:23555888</ref>
-	~~[[4i59]] is~~ a ~~4 chain structure with sequence~~ from ~~[http~~:~~//en~~.~~wikipedia~~.~~org/wiki~~/~~Microbacterium_oxydans Microbacterium oxydans]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id~~=~~4I59 OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Microbacterium oxydans]]		[[Category: Microbacterium oxydans]]
-	[[Category: Berghuis, A M.]]	+	[[Category: Berghuis, A M]]
-	[[Category: Mirza, I A.]]	+	[[Category: Mirza, I A]]
	[[Category: Biocatalysis]]		[[Category: Biocatalysis]]
	[[Category: Cyclohexylamine oxidase]]		[[Category: Cyclohexylamine oxidase]]

OCA at 07:36, 2 May 2013

2013-05-02T07:36:43Z

←Older revision		Revision as of 07:36, 2 May 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4i59\| PDB=4i59 \| SCENE= }}
		+	===Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone===
		+	{{ABSTRACT_PUBMED_23555888}}

-	~~The entry~~ 4i59 is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4i59]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Microbacterium_oxydans Microbacterium oxydans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I59 OCA].
-	~~Authors~~: ~~Mirza~~, I.A., ~~Berghuis,~~ A.M.	+	[[Category: Microbacterium oxydans]]
-		+	[[Category: Berghuis, A M.]]
-	~~Description~~: Cyclohexylamine ~~Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone~~	+	[[Category: Mirza, I A.]]
		+	[[Category: Biocatalysis]]
		+	[[Category: Cyclohexylamine oxidase]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Monoamine oxidase]]
		+	[[Category: Oxidoreductase]]

OCA at 10:49, 24 April 2013

2013-04-24T10:49:13Z

←Older revision		Revision as of 10:49, 24 April 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4i59 is ON HOLD until ~~sometime in the future~~	+	The entry 4i59 is ON HOLD until Paper Publication

	Authors: Mirza, I.A., Berghuis, A.M.		Authors: Mirza, I.A., Berghuis, A.M.

	Description: Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone		Description: Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone

OCA at 08:52, 2 January 2013

2013-01-02T08:52:36Z

←Older revision		Revision as of 08:52, 2 January 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4i59 is ON HOLD	+	The entry 4i59 is ON HOLD until sometime in the future

	Authors: Mirza, I.A., Berghuis, A.M.		Authors: Mirza, I.A., Berghuis, A.M.

	Description: Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone		Description: Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone

OCA: Protected "4i59" [edit=sysop:move=sysop]

2012-12-05T10:34:50Z

Protected "4i59" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:34, 5 December 2012

OCA: New page: '''Unreleased structure''' The entry 4i59 is ON HOLD Authors: Mirza, I.A., Berghuis, A.M. Description: Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohe...

2012-12-05T10:34:49Z

New page: '''Unreleased structure''' The entry 4i59 is ON HOLD Authors: Mirza, I.A., Berghuis, A.M. Description: Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohe...

New page

'''Unreleased structure'''

The entry 4i59 is ON HOLD

Authors: Mirza, I.A., Berghuis, A.M.

Description: Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A complexed with cyclohexanone