4ioi - Revision history

OCA at 21:00, 16 November 2022

2022-11-16T21:00:31Z

←Older revision		Revision as of 21:00, 16 November 2022
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	==Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC==		==Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC==
-	<StructureSection load='4ioi' size='340' side='right' caption='[[4ioi]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='4ioi' size='340' side='right'caption='[[4ioi]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ioi]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"diplococcus_magnus"_prevot_1933 "diplococcus magnus" prevot 1933~~], [~~http~~://en.wikipedia.org/wiki/~~Human Human~~] and [~~http~~://en.wikipedia.org/wiki/~~Staam Staam~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IOI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4IOI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ioi]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IOI FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hjg\|4hjg]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ioi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ioi OCA], [https://pdbe.org/4ioi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ioi RCSB], [https://www.ebi.ac.uk/pdbsum/4ioi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ioi ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spa, SAV0111 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ioi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ioi OCA], [~~http~~://pdbe.org/4ioi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ioi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ioi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ioi ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPA_STAAM SPA_STAAM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4ioi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4ioi" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Diplococcus magnus prevot 1933~~]]	+	[[Category: Finegoldia magna]]
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Staam~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Avery, K N~~]]	+	[[Category: Staphylococcus aureus subsp. aureus Mu50]]
-	[[Category: ~~Bzymek, K P~~]]	+	[[Category: Avery KN]]
-	[[Category: ~~Williams, J C~~]]	+	[[Category: Bzymek KP]]
-	[[Category: ~~Zer, C~~]]	+	[[Category: Williams JC]]
-	[[Category: ~~Cancer immunotherapy]]~~	+	[[Category: Zer C]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Monoclonal antibody~~]]	+

OCA at 02:06, 5 August 2016

2016-08-05T02:06:46Z

←Older revision		Revision as of 02:06, 5 August 2016
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		+
	==Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC==		==Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC==
	<StructureSection load='4ioi' size='340' side='right' caption='[[4ioi]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4ioi' size='340' side='right' caption='[[4ioi]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ioi]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Finegoldia_magna Finegoldia magna~~], [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~] and [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IOI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ioi]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_magnus"_prevot_1933 "diplococcus magnus" prevot 1933], [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IOI FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hjg\|4hjg]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hjg\|4hjg]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spa, SAV0111 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 ~~Staphylococcus aureus subsp. aureus Mu50~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spa, SAV0111 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ioi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ioi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ioi RCSB], [http://www.ebi.ac.uk/pdbsum/4ioi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ioi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ioi OCA], [http://pdbe.org/4ioi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ioi RCSB], [http://www.ebi.ac.uk/pdbsum/4ioi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ioi ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ioi" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Finegoldia magna~~]]	+	[[Category: Diplococcus magnus prevot 1933]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
-	[[Category: ~~Staphylococcus aureus subsp. aureus mu50~~]]	+	[[Category: Staam]]
	[[Category: Avery, K N]]		[[Category: Avery, K N]]
	[[Category: Bzymek, K P]]		[[Category: Bzymek, K P]]

OCA at 12:53, 21 December 2014

2014-12-21T12:53:02Z

←Older revision		Revision as of 12:53, 21 December 2014
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-	~~{{STRUCTURE_4ioi\| PDB=4ioi \| SCENE= }}~~	+	==Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC==
-	===Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC===	+	<StructureSection load='4ioi' size='340' side='right' caption='[[4ioi]], [[Resolution\|resolution]] 1.95Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24101516}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ioi]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IOI FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hjg\|4hjg]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spa, SAV0111 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 Staphylococcus aureus subsp. aureus Mu50])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ioi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ioi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ioi RCSB], [http://www.ebi.ac.uk/pdbsum/4ioi PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Capitalizing on their extraordinary specificity, monoclonal antibodies (mAbs) have become one of the most reengineered classes of biological molecules. A major goal in many of these engineering efforts is to add new functionality to the parental mAb, including the addition of cytotoxins and imaging agents for medical applications. Herein, we present a unique peptide-binding site within the central cavity of the fragment antigen binding framework region of the chimeric, anti-epidermal growth factor receptor mAb cetuximab. We demonstrate through diffraction methods, biophysical studies, and sequence analysis that this peptide, a meditope, has moderate affinity for the Fab, is specific to cetuximab (i.e., does not bind to human IgGs), and has no significant effect on antigen binding. We further demonstrate by diffraction studies and biophysical methods that the meditope binding site can be grafted onto the anti-human epidermal growth factor receptor 2 mAb trastuzumab, and that the antigen binding affinity of the grafted trastuzumab is indistinguishable from the parental mAb. Finally, we demonstrate a bivalent meditope variant binds specifically and stably to antigen-bearing cells only in the presence of the meditope-enabled mAbs. Collectively, this finding and the subsequent characterization and engineering efforts indicate that this unique interface could serve as a noncovalent "linker" for any meditope-enabled mAb with applications in multiple mAb-based technologies including diagnostics, imaging, and therapeutic delivery.

-	~~==About this Structure==~~	+	Identification and grafting of a unique peptide-binding site in the Fab framework of monoclonal antibodies.,Donaldson JM, Zer C, Avery KN, Bzymek KP, Horne DA, Williams JC Proc Natl Acad Sci U S A. 2013 Oct 7. PMID:24101516<ref>PMID:24101516</ref>
-	~~[[4ioi]] is~~ a ~~5 chain structure with sequence from [http://en~~.~~wikipedia.org/wiki/Finegoldia_magna Finegoldia magna]~~, ~~[http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens] and [http~~:~~//en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4IOI OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024101516</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Finegoldia magna]]		[[Category: Finegoldia magna]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Staphylococcus aureus subsp. aureus mu50]]		[[Category: Staphylococcus aureus subsp. aureus mu50]]
-	[[Category: Avery, K N.]]	+	[[Category: Avery, K N]]
-	[[Category: Bzymek, K P.]]	+	[[Category: Bzymek, K P]]
-	[[Category: Williams, J C.]]	+	[[Category: Williams, J C]]
-	[[Category: Zer, C.]]	+	[[Category: Zer, C]]
	[[Category: Cancer immunotherapy]]		[[Category: Cancer immunotherapy]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Monoclonal antibody]]		[[Category: Monoclonal antibody]]

OCA at 05:20, 23 October 2013

2013-10-23T05:20:50Z

←Older revision		Revision as of 05:20, 23 October 2013
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	{{STRUCTURE_4ioi\| PDB=4ioi \| SCENE= }}		{{STRUCTURE_4ioi\| PDB=4ioi \| SCENE= }}
	===Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC===		===Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC===
		+	{{ABSTRACT_PUBMED_24101516}}

	==About this Structure==		==About this Structure==
	[[4ioi]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IOI OCA].		[[4ioi]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IOI OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024101516</ref><references group="xtra"/><references/>
	[[Category: Finegoldia magna]]		[[Category: Finegoldia magna]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 06:38, 10 October 2013

2013-10-10T06:38:24Z

←Older revision		Revision as of 06:38, 10 October 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4ioi\| PDB=4ioi \| SCENE= }}
		+	===Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC===

-	~~The entry~~ 4ioi is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4ioi]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IOI OCA].
-	~~Authors~~: ~~Bzymek, K~~.P., ~~Zer, C~~., Avery, K.N., Williams, J.C.	+	[[Category: Finegoldia magna]]
-		+	[[Category: Homo sapiens]]
-	~~Description~~: ~~Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC~~	+	[[Category: Staphylococcus aureus subsp. aureus mu50]]
		+	[[Category: Avery, K N.]]
		+	[[Category: Bzymek, K P.]]
		+	[[Category: Williams, J C.]]
		+	[[Category: Zer, C.]]
		+	[[Category: Cancer immunotherapy]]
		+	[[Category: Immune system]]
		+	[[Category: Monoclonal antibody]]

OCA at 11:12, 23 January 2013

2013-01-23T11:12:21Z

←Older revision		Revision as of 11:12, 23 January 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ioi is ON HOLD	+	The entry 4ioi is ON HOLD until Paper Publication

	Authors: Bzymek, K.P., Zer, C., Avery, K.N., Williams, J.C.		Authors: Bzymek, K.P., Zer, C., Avery, K.N., Williams, J.C.

	Description: Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC		Description: Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC

OCA: Protected "4ioi" [edit=sysop:move=sysop]

2013-01-16T08:27:48Z

Protected "4ioi" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:27, 16 January 2013

OCA: New page: '''Unreleased structure''' The entry 4ioi is ON HOLD Authors: Bzymek, K.P., Zer, C., Avery, K.N., Williams, J.C. Description: Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC

2013-01-16T08:27:47Z

New page: '''Unreleased structure''' The entry 4ioi is ON HOLD Authors: Bzymek, K.P., Zer, C., Avery, K.N., Williams, J.C. Description: Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC

New page

'''Unreleased structure'''

The entry 4ioi is ON HOLD

Authors: Bzymek, K.P., Zer, C., Avery, K.N., Williams, J.C.

Description: Meditope-enabled trastuzumab in complex with CQFDLSTRRLKC